Synergistic interplay between melatonin and hydrogen sulfide enhances cadmium-induced oxidative stress resistance in stock (Matthiola incana L.).

Ornamental crops particularly cut flowers are considered sensitive to heavy metals (HMs) induced oxidative stress condition. Melatonin (MLT) is a versatile phytohormone with the ability to mitigate abiotic stresses induced oxidative stress in plants. Similarly, signaling molecules such as hydrogen sulfide (H2S) have emerged as potential options for resolving HMs related problems in plants. The mechanisms underlying the combined application of MLT and H2S are not yet explored. Therefore, we evaluated the ability of individual and combined applications of MLT (100 µM) and H2S in the form of sodium hydrosulfide (NaHS), a donor of H2S, (1.5 mM) to alleviate cadmium (Cd) stress (50 mg L-1) in stock (Matthiola incana L.) plants by measuring various morpho-physiological and biochemical characteristics. The results depicted that Cd-stress inhibited growth, photosynthesis and induced Cd-associated oxidative stress as depicted by excessive ROS accumulation. Combined application of MLT and H2S efficiently recovered all these attributes. Furthermore, Cd stress-induced oxidative stress markers including electrolyte leakage, malondialdehyde, and hydrogen peroxide are partially reversed in Cd-stressed plants by MLT and H2S application. This might be attributed to MLT or H2S induced antioxidant plant defense activities, which effectively reduce the severity of oxidative stress indicators. Overall, MLT and H2S supplementation, favorably regulated Cd tolerance in stock; yet, the combined use had a greater effect on Cd tolerance than the independent application.

Silicon nanoparticles alleviate cadmium toxicity in rice (Oryza sativa L.) by modulating the nutritional profile and triggering stress-responsive genetic mechanisms.

This study investigated the physiological and molecular responses of rice genotype '9311' to Cd stress and the mitigating effects of silicon oxide nanoparticles (SiO NPs). Cd exposure severely hindered plant growth, chlorophyll content, photosynthesis, and Cd accumulation. However, SiO NPs supplementation, particularly the SiONP100 treatment, significantly alleviated Cd-induced toxicity, mitigating the adverse effects on plant growth while maintaining chlorophyll content and photosynthetic attributes. The SiONP100 treatment also reduced Cd accumulation, indicating a preference for Si uptake in genotype 9311. Complex interactions among Cd, Si, Mg, Ca, and K were uncovered, with fluctuations in MDA and H2O2 contents. Distinct morphological changes in stomatal aperture and mesophyll cell structures were observed, including changes in starch granules, grana thylakoids, and osmophilic plastoglobuli. Moreover, following SiONP100 supplementation, genotype 9311 increased peroxidase, superoxide dismutase, and catalase activities by 56%, 44%, and 53% in shoots and 62%, 49%, and 65% in roots, respectively, indicating a robust defense mechanism against Cd stress. Notably, OsNramp5, OsHMA3, OsSOD-Cu/Zn, OsCATA, OsCATB, and OsAPX1 showed significant expression after SiO NPs treatment, suggesting potential Cd translocation within rice tissues. Overall, SiO NPs supplementation holds promise for enhancing Cd tolerance in rice plants while maintaining essential physiological functions.

Transcriptional and biochemical profiling of defense enzymes in Citrus sinensis during salicylic acid and cinnamon mediated suppression of green and blue mold.

Green and blue mold of citrus are threatening diseases that continuously inflict economic post-harvest loss. The suppressive effect of salicylic (SA) and Cinnamomum verum (CV) on green and blue mold of sweet oranges was investigated in this study. Among five tested plant extracts methanolic extract of Cinnamon caused the highest colony growth inhibition of P. digitatum and P. italicum in an in vitro antifungal assay. The methanolic extract of Cinnamon in combination with SA showed the lowest disease incidence and severity of green and blue mold on citrus fruit without affecting the fruit quality. Transcriptional profiling of defense enzymes revealed that the polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), and peroxidase (POD) genes were upregulated in fruit treated with CV, SA, and their combination compared to the control. The treatment SA+CV caused the highest upsurge in PPO, POD, and PAL gene expression than the control. Furthermore, the biochemical quantification of PPO, POD and PAL also revealed a similar pattern of activity. The present findings unravel the fact that the escalation in the activity of tested defense enzymes is possibly associated with the reduced incidence of blue and green molds. In conclusion, the study unveils the promising suppressive potential of SA+CV against green and blue mold by regulating the expression of PPO, POD, and PAL genes. Therefore, these treatments can find a role as safer alternatives to chemicals in the management of post-harvest green and blue mold.

Exogenous nitric oxide promotes salinity tolerance in plants: A meta-analysis.

Nitric oxide (NO) has received much attention since it can boost plant defense mechanisms, and plenty of studies have shown that exogenous NO improves salinity tolerance in plants. However, because of the wide range of experimental settings, it is difficult to assess the administration of optimal dosages, frequency, timing, and method of application and the overall favorable effects of NO on growth and yield improvements. Therefore, we conducted a meta-analysis to reveal the exact physiological and biochemical mechanisms and to understand the influence of plant-related or method-related factors on NO-mediated salt tolerance. Exogenous application of NO significantly influenced biomass accumulation, growth, and yield irrespective of salinity stress. According to this analysis, seed priming and foliar pre-treatment were the most effective methods of NO application to plants. Moreover, one-time and regular intervals of NO treatment were more beneficial for plant growth. The optimum concentration of NO ranges from 0.1 to 0.2 mM, and it alleviates salinity stress up to 150 mM NaCl. Furthermore, the beneficial effect of NO treatment was more pronounced as salinity stress was prolonged (>21 days). This meta-analysis showed that NO supplementation was significantly applicable at germination and seedling stages. Interestingly, exogenous NO treatment boosted plant growth most efficiently in dicots. This meta-analysis showed that exogenous NO alleviates salt-induced oxidative damage and improves plant growth and yield potential by regulating osmotic balance, mineral homeostasis, photosynthetic machinery, the metabolism of reactive oxygen species, and the antioxidant defense mechanism. Our analysis pointed out several research gaps, such as lipid metabolism regulation, reproductive stage performance, C4 plant responses, field-level yield impact, and economic profitability of farmers in response to exogenous NO, which need to be evaluated in the subsequent investigation.

Isolation and characterization of peroxidase P7-like gene and Rab-GDI like gene from potential medicinal plants: A step toward understanding cell defense signaling.

Defensin genes form part of a plant's defense system and are activated when exposed to biotic or abiotic stress. They play a vital role in controlling many signaling pathways involved in various plant defense mechanisms. This research aimed to isolate and characterize novel defensin genes from selected medicinally important plants to explore their signaling mechanisms and defense associated roles for breeding. The DNA of Albizia lebbeck and Moringa oleifera was subjected to PCR amplification using gene-specific primers of defensin genes. Two novel defensin genes were isolated in each species, with sequence lengths of 300 bp in A. lebbeck and 150 bp in M. oleifera. In-silico analysis undertaken to retrieve and align their orthologous sequences revealed 100% similarity of the A. lebbeck gene with the Musa acuminate peroxidase P7-like gene and 85% similarity of the M. oleifera gene with the Manihot esculenta GDP dissociation inhibitor gene. The reliability, stability and physiochemical properties of homology models of these sequences was confirmed through online computational studies. This preliminary study confirmed the presence of novel genes with peroxidase P7 and Rab GDP dissociation inhibitor gene-like activity in A. lebbeck and M. oleifera, respectively, and their potential defense role in plants. Thus, the defensin genes of both species could be used in the synthesis of transgenic self-defensive plants with increased disease resistance and as potential candidates for improved crop production and thraputic formulation in the future.

Antioxidants as modulators of arsenic-induced oxidative stress tolerance in plants: An overview.

Arsenic (As) is a highly toxic contaminant in the environment. Although both inorganic and organic types of arsenic exist in the environment, the most common inorganic forms of As that adversely affect plants are arsenite (As III) and arsenate (As V). Despite no evidence for As being essential for plant growth, exposure of roots to this element can cause its uptake primarily via transporters responsible for the transport of essential mineral nutrients. Arsenic exposure even at low concentrations disturbs the plant normal functioning via excessive generation of reactive oxygen species, a condition known as oxidative stress leading to an imbalance in the redox system of the plant. This is associated with considerable damage to the cell components thereby impairing normal cellular functions and activation of several cell survival and cell death pathways. To counteract this oxidative disorder, plants possess natural defense mechanisms such as chemical species and enzymatic antioxidants. This review considers how different types of antioxidants participate in the oxidative defense mechanism to alleviate As stress in plants. Since the underlying phenomena of oxidative stress tolerance are not yet fully elucidated, the potential for "Omics" technologies to uncover molecular mechanisms are discussed. Various strategies to improve As-induced oxidative tolerance in plants such as exogenous supplementation of effective growth regulators, protectant chemicals, transgenic approaches, and genome editing are also discussed thoroughly in this review.

Biochar, Compost, and Biochar-Compost Blend Applications Modulate Growth, Photosynthesis, Osmolytes, and Antioxidant System of Medicinal Plant Alpinia zerumbet.

Alpinia zerumbet (Zingiberaceae) is a unique ornamental and medicinal plant primarily used in food ingredients and traditional medicine. While organic amendments such as biochar (BC) and compost (Co) have been demonstrated to improve plant productivity, no studies have examined their effects on the growth, physiology, and secondary metabolites of A. zerumbet. This study evaluated the impact of the amendment of BC, Co, or a biochar and compost mixture (BC+Co) on modifying and improving the growth, photosynthesis, antioxidant status, and secondary metabolism of A. zerumbet grown on sandy loam soil. The morpho-physiological and biochemical investigation revealed variation in the response of A. zerumbet to organic amendments. The amendment of BC and BC+Co significantly increased net photosynthetic rates of plants by more than 28%, chlorophyll a and b contents by 92 and 78%, respectively, and carboxylation efficiency by 50% compared with those grown in the sandy loam soil without amendment. Furthermore, the amendment significantly decreased plant oxidative stress, measured as leaf free proline and glycine betaine. Enzymatic antioxidant activity, total phenols, and flavonoids also varied in their response to the organic amendments. In conclusion, this study shows that BC and/or Co amendments are an efficient and sustainable method for improving the metabolite contents and reducing oxidative stress in A. zerumbet.

Selenium Supplementation and Crop Plant Tolerance to Metal/Metalloid Toxicity.

Selenium (Se) supplementation can restrict metal uptake by roots and translocation to shoots, which is one of the vital stress tolerance mechanisms. Selenium can also enhance cellular functions like membrane stability, mineral nutrition homeostasis, antioxidant response, photosynthesis, and thus improve plant growth and development under metal/metalloid stress. Metal/metalloid toxicity decreases crop productivity and uptake of metal/metalloid through food chain causes health hazards. Selenium has been recognized as an element essential for the functioning of the human physiology and is a beneficial element for plants. Low concentrations of Se can mitigate metal/metalloid toxicity in plants and improve tolerance in various ways. Selenium stimulates the biosynthesis of hormones for remodeling the root architecture that decreases metal uptake. Growth enhancing function of Se has been reported in a number of studies, which is the outcome of improvement of various physiological features. Photosynthesis has been improved by Se supplementation under metal/metalloid stress due to the prevention of pigment destruction, sustained enzymatic activity, improved stomatal function, and photosystem activity. By modulating the antioxidant defense system Se mitigates oxidative stress. Selenium improves the yield and quality of plants. However, excessive concentration of Se exerts toxic effects on plants. This review presents the role of Se for improving plant tolerance to metal/metalloid stress.

An overview of plant-based natural biostimulants for sustainable horticulture with a particular focus on moringa leaf extracts.

The horticulture sector is facing various challenges in the near future. Aside from maintaining or even improving yields, sustainable horticulture production is crucial to achieve food security. Reducing the reliance on agro-chemicals and/or increasing the efficiency of use under a changing climate is crucial. Natural biostimulants can play an important role in this regard, increasing production at a relatively low cost sustainably. Natural biostimulant feedstocks include leaf, root or seed extracts, either individually or in combination with others. Their positive effect on horticultural production is mostly due to plant growth-enhancing bioactive compounds such as phytohormones, amino acids, and nutrients. Here we review recent progress made in research and applications on plant-derived extracts with an emphasis on the use of these renewable biochemicals as biostimulants in sustainable horticulture. Moringa leaf extracts in particular have been shown to improve seed germination, plant growth and yield, nutrient use efficiency, crop and product quality traits (pre- and post-harvest), as well as tolerance to abiotic stresses. Although horticulture production relies on synthetic fertilisers to maintain and improve production, the use of plant-derived biostimulants such as moringa leaf extracts may be an option to reduce quantities needed and thus contribute in achieving global food security sustainably.