Bridelia ferruginea inhibits key carbohydrate digesting enzyme and intestinal glucose absorption and modulates glucose metabolism in diabetic rats.

The antidiabetic potentials of the dichloromethene, ethyl acetate, butanol and aqueous fractions of Bridelia ferruginea leaves were investigated using in vitro, ex vivo and in vivo models. In vitro and ex vivo antidiabetic activities revealed the butanol (BFBF) to be the most active of the fractions, and thus selected for in vivo study. Diabetes was induced using the fructose-streptozotocin model. Treatments with BFBF significantly reduced blood glucose level and improved glucose tolerance, serum insulin level and sensitivity as well as suppressed hyperlipidaemia and serum nephropathy markers. Histopathological analysis revealed the ability of BFBF to protect and regenerate pancreatic ß-cells. BFBF significantly elevated glutathione level, catalase and superoxide dismutase activities, while depleting MDA level in serums and kidney of diabetic rats. Phenols, steroids, terpenoids, aliphatic and aromatic compounds were identified in the fractions following GC-MS analysis. Overall, results from this study propose that BFBF possess potent antidiabetic activity.

In vitro and computational studies of the antioxidant and anti-diabetic properties of Bridelia ferruginea.

The leaves, stem and root bark of Bridelia ferruginea were sequentially extracted with solvents of increasing polarity to yield the hexane, ethyl acetate, ethanol and aqueous extracts. In vitro analysis revealed the ability of the extracts to scavenge 1,1-diphenyl-2-picryl-hydrazyl (DPPH), nitric oxide (NO) and hydroxyl radical. They also inhibited the activities of α-glucosidase, α-amylase and lipase enzymes. Gas chromatography-mass spectroscopic (GC-MS) analysis of the extracts revealed the presence of sterols, aromatics, aliphatic acids and esters. The identified compounds were molecularly docked with α-glucosidase, α-amylase and lipase enzymes. All compounds showed good binding affinities with the enzymes studied. The strongest binding affinities were observed for ß-amyrin, 4-phenylbenzophenone and lupenone for α-glucosidase, α-amylase and lipase enzymes, respectively. The data suggest antioxidant and antidiabetic potential of the different parts of B. ferruginea, with the leaves having the highest potential. These properties can be explored for development of novel anti-diabetic drugs.Communicated by Ramaswamy H. Sarma.