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1.
Braz J Microbiol ; 51(4): 1465-1473, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-32638273

RESUMEN

Vulvovaginal candidiasis is a serious health problem affecting numerous women around the world. Its treatment is based on antifungals which may not provide an effective cure because of the resistance presented by its etiological pathogens Candida spp. Candida albicans is the most prevalent species related to vulvovaginal candidiasis. Here, we evaluated the in vivo antifungal potential of thiosemicarbazide and thiosemicarbazide encapsulated within chitosan nanoparticles in a murine model of vulvovaginal candidiasis. The results demonstrated the antifungal capacity of free or nanoencapsulated thiosemicarbazide within chitosan to reduce the fungal load in the vaginal tissue of infected mice. In addition, histological analyses indicated the absence or a mild to moderate infection in thiosemicarbazide-treated groups. Statistical tests confirmed the existence of significant differences between the treated and the control groups. Therefore, our results suggest a potential application of thiosemicarbazide and encapsulated thiosemicarbazide as an alternative vulvovaginal candidiasis therapy.


Asunto(s)
Antifúngicos , Candidiasis Vulvovaginal/tratamiento farmacológico , Semicarbacidas , Animales , Antifúngicos/administración & dosificación , Antifúngicos/farmacología , Candida albicans/efectos de los fármacos , Quitosano , Evaluación Preclínica de Medicamentos , Femenino , Ratones , Ratones Endogámicos BALB C , Nanopartículas , Semicarbacidas/administración & dosificación , Semicarbacidas/farmacología , Vagina/microbiología
2.
Braz J Microbiol ; 51(1): 125-134, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-31833006

RESUMEN

Paracoccidioidomycosis (PCM) is a disease caused by fungi of the genus Paracoccidioides. The disease is responsible for high rates of premature deaths and socioeconomic repercussions. The limitations of antifungal agents against PCM have motivated the search for new compounds. In our ongoing exploration of Cerrado plants as potential sources of new antifungal agents, we selected Copaifera langsdorffii oil (Copaíba resin oil) in order to explore its bioactive potential and test a formulation to increase oil stability and solubilization employing Pluronic F-127 to obtain the nanoemulsion of the oil. We aim at testing both Copaíba resin oil and its nanoemulsion against four species of the Paracoccidioides genus. We performed cytotoxicity test in Balb/C3T3 cells, hemolytic activity and interaction of Copaíba resin oil and Copaíba resin oil nanoemulsion (CopaPlu) with the antifungal agents such as amphotericin B, co-trimoxazole, and itraconazole. Moreover, the Copaíba resin oil was analyzed by mass spectrometry to identify its chemical profile. Eventually, a new methodology to prepare the nanoemulsion is presented. The Copaíba resin oil and CopaPlu nanoemulsion inhibited Paracoccidioides sp. growth efficiently, and no cytotoxicity or hemolytic effect was observed at minimum inhibitory concentration (MIC). When combined with amphotericin B, Copaíba resin oil and its nanoemulsion showed an additive effect with reduction of MIC values. The Copaíba resin oil and CopaPlu nanoemulsion is a promising antifungal agent against Paracoccidioides.


Asunto(s)
Antifúngicos/farmacología , Emulsiones/farmacología , Fabaceae/química , Nanopartículas/química , Paracoccidioides/efectos de los fármacos , Aceites de Plantas/farmacología , Animales , Línea Celular , Emulsiones/química , Fibroblastos/efectos de los fármacos , Espectrometría de Masas , Ratones , Pruebas de Sensibilidad Microbiana , Aceites Volátiles/farmacología , Aceites de Plantas/química
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