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Métodos Terapéuticos y Terapias MTCI
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Domest Anim Endocrinol ; 24(4): 341-51, 2003 May.
Artículo en Inglés | MEDLINE | ID: mdl-12742551


The objective of the present study was to evaluate the potential immunological benefit of adding menhaden fish oil to the diet of weaned pigs. Twenty-four crossbred male pigs were weaned at approximately 18 days of age and placed on a complex nursery diet containing 30% lactose and 7% plasma protein with 6% corn oil as the fat source (Cont, n=12) or with 5% menhaden fish oil and 1% corn oil as the fat source (MFO, n=12) for a period of 15 days. Body weights did not differ (P>0.78) between dietary groups either at the beginning or end of the 15 days feeding period. On day 15, all pigs were non-surgically fitted with an indwelling jugular catheter. On d 16, pigs received an i.v. injection of either saline (n=6/dietary group) or lipopolysaccharide (LPS; 150 microg/kg body weight; n=6/dietary group) and blood samples were collected at 30 min intervals for a period of 5h. Serum was harvested and stored at -80 degrees C for analysis of cortisol (CS), corticosteroid-binding globulin (CBG), tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). There was no significant effect of diet on basal concentrations (Time 0) of any of the blood parameters analyzed. A Time x Treatment x Diet interaction (P<0.03) was observed for serum CS such that those pigs which consumed the MFO diet followed by LPS treatment had a reduced CS response as compared to the LPS-treated pigs on the Cont diet. A Time x Treatment interaction (P<0.01) was observed for serum CBG such that LPS treatment reduced circulating CBG as compared to the saline-treated pigs. Time x Treatment x Diet interactions were also observed for serum concentrations of TNF-alpha (P=0.084) and IFN-gamma (P=0.022) such that both the TNF-alpha and IFN-gamma response to the LPS challenge was lower in those pigs receiving the MFO diet as compared to the LPS-treated pigs on the Cont diet. Overall, serum CS was negatively correlated with the CBG response (r=-0.40, P<0.001), however, the strongest negative correlation was observed in the LPS-treated pigs which consumed the MFO diet (r=-0.63, P<0.001). While further studies are needed to evaluate the immunological response of including MFO in the nursery pig diet, the present study demonstrates that supplementation with MFO does indeed alter the immunological response to an LPS challenge.

Dieta , Grasas Insaturadas en la Dieta/administración & dosificación , Aceites de Pescado/administración & dosificación , Lipopolisacáridos/administración & dosificación , Porcinos/inmunología , Animales , Proteínas Sanguíneas/administración & dosificación , Peso Corporal , Aceite de Maíz , Escherichia coli , Hidrocortisona/sangre , Interferón gamma/sangre , Cinética , Lactosa/administración & dosificación , Plasma , Porcinos/crecimiento & desarrollo , Transcortina/análisis , Factor de Necrosis Tumoral alfa/análisis , Destete
J Dairy Sci ; 84(3): 641-8, 2001 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-11286418


Transport of retinol (vitamin A alcohol) from retinoid stores in the liver to target tissues is accomplished exclusively by a specific plasma protein, retinol-binding protein. Within individuals, retinol-binding protein concentrations in plasma are regulated and remain constant except in extremes of vitamin A nutriture or in disease. In the present study, retinol-binding protein concentrations in plasma from preruminant calves supplemented with 0, 1700 (i.e., current NRC requirement), 34,000, or 68,000 IU of vitamin A daily from birth to 27 d of age (n = 6/treatment) were quantified. Retinol-binding protein concentrations at birth averaged 21 microg/ml (n = 24) or approximately 50% of concentrations in dairy heifers and cows. Plasma retinol and retinol-binding protein concentrations were correlated positively, corroborating the role of vitamin A nutriture in the regulation of retinol-binding protein secretion from the liver. In this regard, dietary vitamin A influenced positively retinol and retinol-binding protein concentrations and, as a consequence, the degree of saturation of retinol-binding protein with retinol. At 27 d of age, calves fed > or = 34,000 IU of vitamin A had substantially higher retinol and retinol-binding protein concentrations than did calves fed < or = 1700 IU of vitamin A, indicating that dietary vitamin A effects positively vitamin A status. The data also suggest that the current NRC requirement may not be sufficient to assure vitamin A adequacy in preruminant calves. Percent saturation of retionol-binding protein with retinol in all calves was < 35%, much lower than anticipated and suggests that the retinol requirement of vitamin A-responsive tissues exceeded vitamin A availability.

Animales Recién Nacidos/metabolismo , Hígado/metabolismo , Proteínas de Unión al Retinol/metabolismo , Vitamina A/administración & dosificación , Vitamina A/sangre , Factores de Edad , Animales , Bovinos , Femenino , Necesidades Nutricionales , Proteínas Plasmáticas de Unión al Retinol , Vitamina A/metabolismo
Circulation ; 88(5 Pt 2): II413-9, 1993 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-8222187


BACKGROUND: After catheter injury, the neoendothelium that grows is abnormal in morphology and in acetylcholine-induced generation of endothelium-derived relaxing factor (EDRF). Heparin has been shown to have stimulatory effects on vascular endothelial growth in vitro. Its effect in vivo on neoendothelial cell morphology and metabolism after injury has not been described. We investigated the effect of heparin treatment on the neoendothelium formed after injury. METHODS AND RESULTS: Four groups of New Zealand White rabbits were studied. Group 1 rabbits underwent catheter denudation and were killed 4 weeks after injury without receiving treatment (NO Tx, n = 8). Groups 2 and 3 underwent similar aortic injury, received 2 weeks of treatment with either heparin (n = 7) or low molecular weight heparin (LMWH, n = 5), and were killed at 4 weeks. Group 4 underwent sham operation (SHAM, n = 8). EDRF generation was determined by the relaxation of precontracted aortic rings in an organ bath in response to acetylcholine. The heparin-treated group exhibited a significant improvement in acetylcholine-induced relaxation (27%) versus both LMWH-treated (14%, P = .035) and untreated groups (11%, P = .004), although relaxation was only 50% of that observed in the uninjured control vessels (52%, P = .001). The neoendothelium formed in the heparin-treated group exhibited a more normal histological appearance and was aligned with the direction of blood flow as compared with that observed in the untreated or LMWH-treated groups. CONCLUSIONS: These results demonstrate that in vivo heparin administration enhanced the recovery of EDRF generation and augmented normalization of the morphologic appearance of the neoendothelium.

Aorta Torácica/lesiones , Cateterismo/efectos adversos , Endotelio Vascular/lesiones , Heparina de Bajo-Peso-Molecular/uso terapéutico , Heparina/uso terapéutico , Óxido Nítrico/metabolismo , Acetilcolina/farmacología , Animales , Aorta Torácica/fisiología , Endotelio Vascular/fisiología , Hiperplasia , Masculino , Microscopía Electrónica de Rastreo , Conejos , Túnica Íntima/patología