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Biol Mass Spectrom ; 21(11): 590-6, 1992 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-1457472


Using laser microprobe mass analysis (LAMMA), the composition of 'micro-calcifications (dystrophic type)' or 'metastatic calcifications', previously described in rats given high doses of cyclosporin, have been identified. Female Wistar rats given 12.5, 25 or 50 mg kg-1 per day of cyclosporin in olive oil by gastric gavage developed intrarenal calcifications detected by silver nitrate staining (von Kossa) in the outer medulla after two weeks. The calcifications stained red with periodic acid-Schiff reagent and appeared to be intraluminal microliths with concentric laminations. They could be identified by LAMMA as calcium phosphate (hydroxyapatite), indistinguishable from undecalcified bone. These studies indicate that intraluminal obstruction by calcium phosphate microliths, similar to that seen with magnesium depletion or high phosphate diets, may contribute to renal damage in rats given high-dose cyclosporin. LAMMA appears to be an appropriate technique for identifying both the elemental and organic moieities of intrarenal calcium deposits.

Ciclosporina/toxicidad , Nefrocalcinosis/inducido químicamente , Administración Oral , Animales , Ciclosporina/administración & dosificación , Femenino , Rayos Láser , Espectrometría de Masas/métodos , Ratas , Ratas Wistar
Biol Trace Elem Res ; 25(3): 149-85, 1990 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-1698415


Sampling, storing, sample pretreatment, and experimental conditions for selenium (Se) determination in human serum, plasma, and whole blood by X-ray emission spectrometric (XRS) methods are described. Concentration levels in these biological fluids, found by this technique, are discussed and compared to values found by other techniques for the same healthy population group in the same area. XRS analysis of blood from patients with various pathological conditions is reviewed, with special attention to the relation of Se with the concentration level of other essential or nonessential trace elements.

Selenio/sangre , Adulto , Niño , Femenino , Humanos , Masculino , Embarazo , Valores de Referencia , Espectrometría por Rayos X
Matrix ; 10(1): 33-7, 1990 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-2352510


Repeated intraperitoneal injections of lead acetate in rats caused a calcification of the skin of the abdomen near the site of the injections. In the lead-induced calcifications, electron dense collagen bundles could be observed. On the surface of the collagen fibrils, needle-like crystals were visible. With energy-dispersive X-ray analysis, phosphorus, calcium and lead were detected in the electron dense collagen bundles. X-ray maps of the P-K alpha, Ca-K alpha, and Pb-L alpha plus Pb-L beta lines showed an equivalent distribution along the collagen fibrils for phosphorus and calcium. The occurrence of the most electron dense areas in the STEM-image was comparable to the lead distribution. A good correlation existed between the structural and the elemental images of the same area. Although the medicinal use of preparations containing lead is no longer recommended, some are still prescribed. From our results we can conclude that they should not be applied to injured or inflamed skin.

Calcinosis/metabolismo , Calcio/análisis , Colágeno/análisis , Plomo/toxicidad , Fósforo/análisis , Enfermedades de la Piel/metabolismo , Animales , Calcinosis/inducido químicamente , Calcinosis/patología , Colágeno/ultraestructura , Microanálisis por Sonda Electrónica , Inyecciones Intraperitoneales , Plomo/farmacocinética , Masculino , Microscopía Electrónica , Ratas , Ratas Endogámicas , Enfermedades de la Piel/inducido químicamente , Enfermedades de la Piel/patología
Pharmacol Res Commun ; 17(5): 479-88, 1985 May.
Artículo en Inglés | MEDLINE | ID: mdl-4034629


Erythrocyte selenium-dependent glutathione peroxidase activity was measured in psoriatic Danes, before and after their four-week balneological therapy at the Ein-Bokek International Psoriasis Treatment Center, on the Dead-Sea shore in Israel. The drinking water in Ein-Bokek was found to be rich in selenium, a trace element with anticarcinogenic properties and of great importance in human nutrition and health. The most reliable biological parameter for increase in selenium bioavailability is the erythrocytes' glutathione-peroxidase activity. As psoriasis is a proliferative skin disease, the activity of this enzyme was assayed in 35 psoriatic Danes and in 25 long-term local hotel workers, as well as in 34 volunteers drinking low-selenium water. The glutathione peroxidase activity in the psoriatic patients increased significantly during their four-week stay in Ein-Bokek. Erythrocyte glutathione peroxidase activity in the hotel workers was 50% higher than that in the healthy volunteers consuming low-selenium water. A possible role of selenium in psoriasis is suggested.

Eritrocitos/enzimología , Glutatión Peroxidasa/sangre , Psoriasis/enzimología , Selenio/farmacología , Abastecimiento de Agua , Adulto , Balneología , Femenino , Humanos , Israel , Masculino , Persona de Mediana Edad , Psoriasis/terapia , Selenio/análisis , Abastecimiento de Agua/análisis
Sci Total Environ ; 26(2): 163-72, 1983 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-6844915


The tetravalent and hexavalent selenium content of water samples can be determined by conventional energy-dispersive X-ray fluorescence after different preconcentration steps. Selenium values of nearly three-hundred different environmental and drinking water samples in Belgium are reported. The results are quite low, ranging from the detection limits up to 1 microgram 1-1. The concentration levels are compared to literature data and the speciation is discussed. The contribution of drinking water to the daily intake of selenium in Belgium ranges from less than 0.2 to 5%.

Selenio/análisis , Contaminantes Químicos del Agua/análisis , Contaminantes del Agua/análisis , Abastecimiento de Agua , Bélgica , Ingestión de Líquidos , Humanos , Selenio/envenenamiento
Pharmacology ; 25(6): 297-307, 1982.
Artículo en Inglés | MEDLINE | ID: mdl-7156179


Serum bromine levels in psoriatic Danes increased 2- to 3-fold during a 4-week bathing course in the Dead Sea. This increase correlated well with the improvement in their clinical and psychic condition. Serum bromine levels in psoriatic Danes were somewhat lower than those in healthy subjects residing in Denmark, but the difference was not significant. Israelis working in the open air in the Dead Sea area (air bromine 20-fold higher than in Jerusalem) had higher bromine levels than psoriatic or healthy Israelis residing in Jerusalem or healthy Israelis working in air-conditioned rooms in the Dead Sea area (p less than 0.05), but those levels were still within the normal range. As our animal experimentation indicates that the skin is a major target organ for 82Br, applied either by bathing or as an aerosol, we conclude that the higher bromine levels noticed in the psoriatic Danes after their 4-week stay at the Dead Sea may be equally due to their contact with the bromine-containing aerosol and the high bromine level of the Dead Sea waters.

Bromo/sangre , Psoriasis/sangre , Adulto , Aerosoles , Aire/análisis , Animales , Balneología , Femenino , Cobayas , Humanos , Israel , Masculino , Persona de Mediana Edad , Radioisótopos , Distribución Tisular