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J Exp Bot ; 63(10): 3727-40, 2012 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-22442419


Seed yield and oil content are two important agricultural characteristics in oil crop breeding, and a lot of functional gene research is being concentrated on increasing these factors. In this study, by differential gene expression analyses between rapeseed lines (zy036 and 51070) which exhibit different levels of seed oil production, BnGRF2 (Brassica napus growth-regulating factor 2-like gene) was identified in the high oil-producing line zy036. To elucidate the possible roles of BnGRF2 in seed oil production, the cDNA sequences of the rapeseed GRF2 gene were isolated. The Blastn result showed that rapeseed contained BnGRF2a/2b which were located in the A genome (A1 and A3) and C genome (C1 and C6), respectively, and the dominantly expressed gene BnGRF2a was chosen for transgenic research. Analysis of 35S-BnGRF2a transgenic Arabidopsis showed that overexpressed BnGRF2a resulted in an increase in seed oil production of >50%. Moreover, BnGRF2a also induced a >20% enlargement in extended leaves and >40% improvement in photosynthetic efficiency because of an increase in the chlorophyll content. Furthermore, transcriptome analyses indicated that some genes associated with cell proliferation, photosynthesis, and oil synthesis were up-regulated, which revealed that cell number and plant photosynthesis contributed to the increased seed weight and oil content. Because of less efficient self-fertilization induced by the longer pistil in the 35S-BnGRF2a transgenic line, Napin-BnGRF2a transgenic lines were further used to identify the function of BnGRF2, and the results showed that seed oil production also could increase >40% compared with the wild-type control. The results suggest that improvement to economically important characteristics in oil crops may be achieved by manipulation of the GRF2 expression level.

Brassica napus/metabolismo , Fotosíntesis , Aceites de Plantas/metabolismo , Proteínas de Plantas/metabolismo , Semillas/citología , Regulación hacia Arriba , Secuencia de Aminoácidos , Brassica napus/química , Brassica napus/citología , Brassica napus/genética , Recuento de Células , Proliferación Celular , Regulación de la Expresión Génica de las Plantas , Datos de Secuencia Molecular , Proteínas de Plantas/química , Proteínas de Plantas/genética , Semillas/química , Semillas/genética , Semillas/metabolismo , Alineación de Secuencia
Plant J ; 69(3): 432-44, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-21954986


Seed oil content is an important agronomic trait in rapeseed. However, our understanding of the regulatory processes controlling oil accumulation is still limited. Using two rapeseed lines (zy036 and 51070) with contrasting oil content, we found that maternal genotype greatly affects seed oil content. Genetic and physiological evidence indicated that difference in the local and tissue-specific photosynthetic activity in the silique wall (a maternal tissue) was responsible for the different seed oil contents. This effect was mimicked by in planta manipulation of silique wall photosynthesis. Furthermore, the starch content and expression of the important lipid synthesis regulatory gene WRINKLED1 in developing seeds were linked with silique wall photosynthetic activity. 454 pyrosequencing was performed to explore the possible molecular mechanism for the difference in silique wall photosynthesis between zy036 and 51070. Interestingly, the results suggested that photosynthesis-related genes were over-represented in both total silique wall expressed genes and genes that were differentially expressed between genotypes. A potential regulatory mechanism for elevated photosynthesis in the zy036 silique wall is proposed on the basis of knowledge from Arabidopsis. Differentially expressed ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)-related genes were used for further investigations. Oil content correlated closely with BnRBCS1A expression levels and Rubisco activities in the silique wall, but not in the leaf. Taken together, our results highlight an important role of silique wall photosynthesis in the regulation of seed oil content in terms of maternal effects.

Brassica napus/genética , Flores/fisiología , Fotosíntesis/fisiología , Aceites de Plantas/química , Semillas/química , Brassica napus/fisiología , Etiquetas de Secuencia Expresada , Flores/metabolismo , Regulación de la Expresión Génica de las Plantas , Biblioteca de Genes , Genes de Plantas , Genotipo , ARN de Planta/genética , Ribulosa-Bifosfato Carboxilasa/metabolismo , Análisis de Secuencia de ADN , Almidón/biosíntesis , Transcriptoma