Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
1.
Curr Med Sci ; 39(2): 211-216, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-31016512

RESUMEN

Discontinuation of tyrosine kinase inhibitor (TKI) therapy after achieving a persistent deep molecular response (DMR) is an urgently needed treatment goal for chronic myeloid leukemia (CML) patients and has been included in the National Comprehensive Cancer Network (NCCN) guidelines (version 2.2017) for CML. Indeed, various studies have confirmed the feasibility of discontinuing TKI therapy. In this study, we analyzed data from 45 CML patients who had discontinued TKI therapy. Univariate analysis was performed to predict factors that were potentially related to treatment-free remission (TFR) and identify the differences between early relapse and late relapse. Out of the 45 patients, 20 exhibited molecular relapse after a median follow-up of 18 months (range, 1-54 months), and the estimated TFR at 24 months was 40%. The univariate analysis revealed that a high Sokal score and interruptions or dose reductions during TKI treatment were the only baseline factors associated with poor outcomes. Our results indicate that TKI discontinuation could be successfully put into practice in China.


Asunto(s)
Leucemia Mielógena Crónica BCR-ABL Positiva/tratamiento farmacológico , Inhibidores de Proteínas Quinasas/uso terapéutico , Proteínas Tirosina Quinasas/antagonistas & inhibidores , Adolescente , Adulto , Anciano , Pueblo Asiatico , China , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven
2.
Nat Prod Res ; 26(14): 1363-7, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-21809946

RESUMEN

This study investigated the antimicrobial and antioxidant activity of the extracts of the flowers, essential oil (EO) and semi-volatile fractions (SVF) of Chimonanthus praecox. The chemical composition of the EO was analysed by gas chromatography-mass spectroscopy (GC-MS) which revealed that the EO contained elemene, muurolene, caryophyllene, cadinol and spathulenol. The effective antibacterial activity of the EO suggested that its different responses on the microorganisms studied depended on the synergistic effects of the compounds contained in the EO. The effective antioxidant activity of the EO showed that the EO had a more marked antioxidant effect on scavenging O2(-)· and OH· than DPPH·, and SVF had a higher potential for scavenging DPPH· than the EO. Our data suggested that the flowers of Chimonanthus praecox had pharmaceutical benefits, and are also a potential source of natural antioxidants and biocides.


Asunto(s)
Antiinfecciosos/química , Antioxidantes/química , Calycanthaceae/química , Flores/química , Extractos Vegetales/química , Antiinfecciosos/farmacología , Compuestos de Bifenilo/química , Pruebas de Sensibilidad Microbiana , Picratos/química , Extractos Vegetales/farmacología
3.
Zhonghua Yi Xue Za Zhi ; 89(7): 480-4, 2009 Feb 24.
Artículo en Chino | MEDLINE | ID: mdl-19567099

RESUMEN

OBJECTIVE: To construct an anti-sense cDNA library of human breast cancer cells to screen essential genes with anti-tumor effects on apoptosis of human breast cancer cells induced by trichostatin A. METHODS: Poly (A)(+)RNA was extracted from human breast cancer cells of the line MCF-7 treated by trichostatin A for 0, 12, 24, 36, 48, 60, or 72 h. cDNA were synthesized and inserted reversely into PCEP 4 vector to construct an anti-sense cDNA library. HeLa cells were transfected with the library DNA or blank PCEP 4 vector as control group. All the transfected cells were screened by 200 nmol/L trichostatin A and 200 microg/ml hygromycin B. Screening was stopped when the control cells died. Then the surviving cell clones were amplified and Hirt DNA was extracted. Several expressed sequence tags were thus obtained. The data were analyzed by bioinformatics and interested EST fragment was chosen for preliminary functional screening. RESULTS: An anti-sense cDNA library was constructed containing 2 x 10(6) independent clones with an insert efficiency of more than 90%; DNA sequencing and bioinformatic analysis suggested that the No.27 survival clone was zinc transporter LIV1 showing a strong resistance against trichostatin A-induced apoptosis during functional screening. CONCLUSION: An anti-sense cDNA library with high quantity and quality has been successfully constructed; LIV1 gene may be one of the essential genes with anti-tumor effects on apoptosis induced by trichostatin A.


Asunto(s)
Apoptosis/genética , Biblioteca de Genes , Ácidos Hidroxámicos/farmacología , Inhibidores de la Síntesis de la Proteína/farmacología , Secuencia de Bases , Clonación Molecular , ADN Complementario/genética , Femenino , Perfilación de la Expresión Génica , Células HeLa , Humanos , Análisis de Secuencia de ADN , Transfección
4.
Acta Pharmacol Sin ; 26(10): 1265-73, 2005 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-16174445

RESUMEN

AIM: To investigate the anticancer effects and the molecular mechanisms of deguelin on human U937 leukemia cells, and to explore the underlying mechanism regulating nucleoporin 98 (Nup98) and nucleoporin 88 (Nup88) in vitro. METHODS: The effects of deguelin on the growth of U937 cells were studied by MTT assay. The effect of deguelin on the cell cycle of U937 cells was studied by using a propidium iodide method. The localization of the nuclear pore complex proteins Nup98 and Nup88 was investigated by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 and Nup88 in U937 cells was investigated by using flow cytometry and Western blot. RESULTS: The proliferation of U937 cells was inhibited in the deguelin-treated group, with a 24-h IC(50) value of 21.61 nmol/L and a 36-h IC(50) value of 17.07 nmol/L. U937 cells treated with deguelin had reduced percentages of cells in the G(0)/G(1) phase, whereas cells accumulated in the S and G(2)/M phases. Nup88 and Nup98 were found on both the nuclear and cytoplasmic sides of the U937 cells by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 was upregulated and that of the Nup88 protein was downregulated in U937 cells treated with deguelin. CONCLUSION: Deguelin is able to inhibit the proliferation of U937 cells by regulating the cell cycle such that cells are arrested at the S and G(2)/M phases, so that the proportion of cells in the G(0)/G(1) phase decreases. The antitumor effects of deguelin are related to upregulating the expression of Nup98 and downregulating the expression of Nup88 protein in U937 cells.


Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Proliferación Celular/efectos de los fármacos , Proteínas de Complejo Poro Nuclear/metabolismo , Rotenona/análogos & derivados , Antineoplásicos Fitogénicos/administración & dosificación , Ciclo Celular , Relación Dosis-Respuesta a Droga , Fabaceae/química , Humanos , Plantas Medicinales/química , Rotenona/administración & dosificación , Rotenona/aislamiento & purificación , Rotenona/farmacología , Células U937
5.
Chin J Integr Med ; 11(4): 283-6, 2005 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-16417779

RESUMEN

OBJECTIVE: To explore the effects and possible mechanisms of Guiqi Oral Liquid (GQOL) on the recovery of hematopoiesis in acute irradiation injured mice. METHODS: The acute irradiation injured mice were randomly divided into 2 groups: the treated group and the control group, and also a normal control group was set up with 6 mice in it receiving no treatment. After the mice in the former two groups were irradiated by 6.0 Gy (60)Co gamma-ray, every one of them was given 0.4 ml GQOL or saline in equal volume through a gastric tube twice a day for 14 days. On the 4th, 8th and 14th day after irradiation, the bone marrow mononuclear cells (BMMNC) and megakaryocytes in bone marrow tissues of the mice were counted, the proportion of hematopoietic tissues (by area) was measured, and the expression of adhesion molecules, CD44 and CD54, in bone marrow were estimated by immunochemistry. The colony forming unit of spleen (CFU-S) in the mice were counted on the 8th day after irradiation. RESULTS: On the 4th, 8th, 14th day after irradiation, the count of BMMNC and megakaryocyte, and the proportion of hematopoietic tissues in the treated group were higher than those in the control group (P < 0.01 or P < 0.05). CD44 and CD54 expression in the treated group were higher than those in the control group on the 4th and 8th day (P < 0.01), but near normal on the 14th day (P < 0.01). On the 8th day, CFU-S count in the treated group was higher than that in the control group (P < 0.01). CONCLUSION: GQOL can regulate the expression of adhesion molecules, CD44 and CD54, in the bone marrow of the acute irradiation injured mice, which may be one of the mechanisms of GQOL in accelerating the early phase hematopoiesis recovery of mice.


Asunto(s)
Angelica sinensis , Astrágalo (Planta) , Medicamentos Herbarios Chinos/farmacología , Hematopoyesis/efectos de los fármacos , Fitoterapia , Traumatismos Experimentales por Radiación/dietoterapia , Animales , Células de la Médula Ósea/citología , Recuento de Células , Receptores de Hialuranos/análisis , Inmunohistoquímica , Molécula 1 de Adhesión Intercelular/análisis , Leucocitos Mononucleares/citología , Masculino , Megacariocitos/citología , Ratones , Traumatismos Experimentales por Radiación/fisiopatología , Distribución Aleatoria
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA