Nano-Selenium Antagonized Cadmium-Induced Liver Fibrosis in Chicken.

Cadmium is a global ecological toxic pollutant; in animals, hepatotoxic fibrosis is caused by bioaccumulation of Cd through food chains. We determined the path of nano-Se antagonism in Cd-induced hepatocyte pyroptosis by targeting the APJ-AMPK-PGC1α pathway, using an in vivo model of hepatotoxicity. All 1-day-old chicks were treated with Cd (140 mg/kg BW/day) and/or nano-Se (0.3 or 0.6 mg/kg BW/day) for 90 days. The result showed that Cd (1.55 ± 0.148) activated NLRP3 inflammasome 49.903% as compared to the Con group (1.034 ± 0.008) to release the inflammasome as a result of hepatocyte pyroptosis (2.824 ± 0.057). Compared with the Con group (1.010 ± 0.021), Kupffer cells were 219.109% more to activate astrocytes through the APJ-AMPK-PGC1α pathway, resulting in 185.149% more hepatic fibrosis. However, the fibrosis degree of the H-Se + Cd group (1.252 ± 0.056) was 56.5278% (p < 0.001) lower than that of the Cd group (2.880 ± 0.124). Therefore, this study established that pyroptotic hepatocytes and Kupffer cells could be targeted for nano-Se antagonizing Cd toxicity, which reveals a potential new approach targeting astrocytes for the treatment of liver fibrosis triggered by Cd pollution.

Phytoremediation potential of hybrids of the exotic plant Xanthium strumarium and its native congener Xanthium sibiricum for cadmium-contaminated soils.

Exotic plants could play an essential role in the restoration of heavy metal-contaminated soil. This study evaluated the tolerance of and extraction of cadmium (Cd) by ZCR (CR♀ × LT♂), hybrids of Xanthium strumarium (LT, exotic species) and X. sibiricum (CR, indigenous congener), and their parental species under different Cd treatments (0, 10, 40, and 80 mg·kg-1). The results showed that the hybrids had significantly improved tolerance to Cd. Under Cd stress, the biomass of ZCR increased by more than 50% on average compared with that of CR. Moreover, the hybrids showed a more remarkable ability to transport Cd from the root to the shoot. The Cd content of the shoots of ZCR increased by 128.33, 147.22, and 252.63% when treated with 10, 40, and 80 mg·kg-1 Cd, respectively. ZCR stored more than 70% of Cd in litter leaves, thereby reducing the toxic effects of Cd on photosynthesis and growth. The results showed that ZCR showed excellent Cd tolerance and enrichment in the presence of Cd. The hybrids of Xanthium strumarium and its native congener X. sibiricum may remediate soil Cd pollution.Novelty statementWith the changing world economy and increasing human activities, exotic plants have become a global issue of common concern to the international community. This study describes new findings on using hybrids of the exotic plant of Xanthium strumarium and its native congener Xanthium sibiricum for the restoration of cadmium-contaminated soils. Under Cd stress, the hybrids' biomass, tolerance, and ability to accumulate Cd were significantly higher than that of X. sibiricum, indicating that hybrids gained useful heavy metal extraction traits from X. strumarium.

Enhancement of the Cd phytoremediation efficiency of Festuca arundinacea by sonic seed treatment.

It has been reported that both naturally occurring and artificially created sounds can alter the physiological parameters of various plants. A series of experiments were designed in the present study to estimate the physiological responses and the variation in the Cd decontamination capacity of Festuca arundinacea under sonic wave treatments. Plant seeds were treated by sound waves of frequency 200, 300, 400, 500, and 1000 Hz, and the germinated seedlings were transplanted to Cd-polluted soil. The results showed that all the sonic treatments increased the whole plant dry weight of F. arundinacea compared with that of the control, and the highest value was observed in the 200 Hz treatment. The Cd content in below-ground and aerial tissues of the species increased with increasing frequency till 400 Hz, after which they became constant. A higher proportion of senescent and dead leaf tissues was observed in the high-frequency treatment (1000 Hz), and more Cd was transferred to these failing tissues. Therefore, in the 1000 Hz treatment, a significantly greater amount of Cd could be eliminated by harvesting the senescent and dead leaf tissues of the species compared with that of the other treatments. The concentrations of dissolved organic matter (DOM) and the proportions of hydrophilic fractions which have a strong Cd affinity, in the rhizosphere soil of F. arundinacea increased with the increase in sound frequency. Cd extraction ability of DOM also increased with increasing frequency. This study indicated that a suitable sonic treatment can improve the phytoextraction efficiency of F. arundinacea, and also explained the mechanism from the perspective of the variations in soil DOM.

Influences of arbuscular mycorrhizae, phosphorus fertiliser and biochar on alfalfa growth, nutrient status and cadmium uptake.

The objective of the study was to explore the influences of arbuscular mycorrhizae (AM), phosphorus (P) fertiliser, biochar application (BC) and their interactions on Medicago sativa growth, nutrient, Cd content and AM fungi-plant symbioses. Applications of both P fertiliser and BC significantly increased total biomass and P and potassium (K) uptake, regardless of AM. When no P fertiliser or BC was used, the shoot biomass and nitrogen (N), P, and K contents in the +AM treatments were 1.39, 1.54, 4.53 and 2.06 times higher than those in the -AM treatments, respectively. AM fungi only elevated the total P uptake by 44.03% when P fertiliser was applied at a rate of 30 mg P kg-1 in the absence of BC addition. With BC application or high-P fertiliser input (100 mg P kg-1), the soil available P was significantly higher than that in the other treatments, and AM fungi significantly reduced the shoot biomass. The minimum Cd concentration occurred in the shoots of alfalfas treated with BC and high-P fertiliser inputs; this concentration was lower than the maximum permitted concentration in China. Although the BC and high-P inputs could eliminate the positive mycorrhizal response, the results suggested that BC application in combination with high-P fertiliser input could not only increase forage yields but also lower Cd concentrations to meet the forage safety standards by the dilution effect.

Growth and elemental uptake of Trifolium repens in response to biochar addition, arbuscular mycorrhizal fungi and phosphorus fertilizer applications in low-Cd-polluted soils.

The aim of this study was to examine the effects of arbuscular mycorrhizal (AM) fungi, biochar (BC) addition and phosphorus (P) fertilizer applications on the mycorrhizal response, biomass and elemental uptake of Trifolium repens in cadmium (Cd)-polluted soils. The results showed that mycorrhizal colonization were significantly decreased by 100 mg P kg-1 fertilizer input. Moreover, AM fungi, BC addition and P fertilizer significantly increased shoot biomass accumulation at all treatments. In the absence of BC, the nitrogen (N), potassium (K), calcium (Ca) and magnesium (Mg) contents in the shoots were not affected by AM fungi after P fertilizer application, but the P content in the shoots significantly increased in response to AM fungi. In the absence of BC, both AM fungi and P fertilizer significantly reduced the Cd concentrations in the plant tissues as well as the soil diethylenetriaminepentaacetic acid (DTPA)-Cd concentration. These results indicated that the translocation factors (TFs) were influenced only by BC addition and that the roots could accumulate greater amounts of Cd than the shoots. On the basis of the hygienic standard for feed in China, the shoot Cd concentration in white clover was below the maximum permitted Cd concentration (1 µg g-1) across all treatments. Therefore, it is suggested that no negative mycorrhizal-white clover symbiotic relationships were observed and T. repens could be a suitable forage species for planting in soils with low concentrations of Cd contamination when BC and P fertilizer are applied.