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Microb Cell Fact ; 20(1): 31, 2021 Feb 02.
Artículo en Inglés | MEDLINE | ID: mdl-33530970

RESUMEN

Chronic atrophic gastritis (CAG) is well-known related with multiple pathogenic factors and normally therapies comprised by western or Chinese medicines. The present study was designed to identify the bacterial community characterized by 16S rRNA amplicon sequencing and determine the modulate affection of bacterial composition response western and Chinese medicine Qinghuayin (QHY) as well as antibiotic on model rats. The result shown the overall structure alteration of bacterial appeared under medicine intervened, antibiotic caused a marked depletion in bacterial diversity and richness. The enrichments of Firmicutes (85.1-90.7%) in antibiotic-free converts into Bacteroidetes (30.7-34.6%) in antibiotic-added model rat were demonstrated. Firmicutes as the most dominant phylum in antibiotic-free treatments and significantly decreased till 21.9-68.5% in antibiotic-added treatments. Especially QHY-treated rats showed highest RA of Firmicutes (90.7%) and the amelioration of CAG using QHY attributed by beneficial bacterial enrichment, especially Ruminococcus, Lactobacillus and Bifidobacterium. In addition, alpha and beta diversity analysis also demonstrated the clear dispersion and aggregation that revealed the alteration and steady of bacterial community structures. In summary, QHY has potential application value in the treatment of CAG, which attributed to close relation with the modulatory of internal bacterial communities.


Asunto(s)
Bacterias/metabolismo , Gastritis Atrófica/microbiología , Gastritis Atrófica/terapia , Microbioma Gastrointestinal , Medicina Tradicional China , Animales , Bacterias/clasificación , Biodiversidad , Enfermedad Crónica , Análisis Discriminante , Modelos Animales de Enfermedad , Masculino , Filogenia , Ratas Wistar
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