Synergistic therapeutic effects of Duzhong Jiangya Tablets and amlodipine besylate combination in spontaneously hypertensive rats using 1 H-NMR- and MS-based metabolomics.

Duzhong Jiangya Tablet (DJT) composed of Eucommia ulmoides Oliv. and several other traditional Chinese medicines is a Chinese herbal compound, which is clinically used to treat hypertension. The aim of this study was to evaluate the antihypertensive effect of DJT and amlodipine besylate (AB) on the synergistic treatment of spontaneously hypertensive rats (SHRs), and to explore its antihypertensive mechanism. The synergistic therapeutic effect of DJT in combination with AB on SHR was studied using two metabolomics methods based on mass spectrum (MS) and nuclear magnetic resonance. Metabolomics analysis of plasma, urine, liver, and kidney and the combination of orthogonal partial least squares discriminant analysis was performed to expose potential biomarkers. Then, the overall metabolic characteristics and related abnormal metabolic pathways in hypertensive rats were constructed. Blood pressure measurements showed that DJT combined with AB has better effects in treating hypertension than it being alone. A total of 30 biomarkers were identified, indicating that hypertension disrupted the balance of multiple metabolic pathways in the body, and that combined administration restored metabolite levels better than their administration alone. The changes of biomarkers revealed the synergistic therapeutic mechanism of DJT combined with AB, which provided a reference for the combination of Chinese and Western medicines.

The Effect of Soothing and Smooth Liver and Nourishing Kidney and Acupuncture Treat Liver-YANG Syndrome Hypertension / 浙江中医药大学学报

Objective] To analyse the effect of combining acupuncture of soothing liver, smooth liver and nourishing kidney with Amlodipine Besylate Tablets on light, medium hyperactivity of liver-YANG syndrome hypertension. [Methods] From January 2013 to January 2014, we collected 60 patients with hyperactivity of liver-YANG syndrome meeting the criterion. 30 patients in control group, taking Amlodipine Besylate Tablets, 14 male, 16 female, average age was 62.0 years old(54~72 years old); 30 cases in treatment group, on the basis of control group using soothing liver, smooth liver and nourishing kidney acupuncture, 13 males, 17 females, average age was 61.5 years old(53~73 years old). Patients were collected 24 hours ambulatory blood pressure, anxiety, TCM syndrome integral before and after treatment. [Results] After treatment, control group and the treatment group of ambulatory blood pressure, anxiety and TCM syndrome integral was significantly improved, the differences were statistically significant( P<0.05). In treatment group, daytime systolic blood pressure, daytime diastolic blood pressure, nighttime systolic blood pressure, nighttime diastolic blood pressure, 24h diastolic blood pressure and 24h systolic blood pressure were decreased significantly lower than the control group, and anxiety value, TCM syndrome integral was also improved better than control group(P<0.05). [Conclusion] Soothing liver, smooth liver and nourishing kidney acupuncture combined with oral drug can effectively control blood pressure for hyperactivity of liver-YANG syndrome hypertension patients; meantime it also can significantly relieve the TCM syndrome and anxiety symptom.