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1.
J Ethnopharmacol ; 272: 113945, 2021 May 23.
Artículo en Inglés | MEDLINE | ID: mdl-33617966

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: Most Aristolochiaceae plants are prohibited due to aristolochic acid nephropathy (AAN), except Xixin (Asarum spp.). Xixin contains trace amounts of aristolochic acid (AA) and is widely used in Traditional Chinese Medicine. Methylglyoxal and d-lactate are regarded as biomarkers for nephrotoxicity. AIM OF THE STUDY: The use of Xixin (Asarum spp.) is essential and controversial. This study aimed to evaluate tubulointerstitial injury and interstitial renal fibrosis by determining urinary methylglyoxal and d-lactate after withdrawal of low-dose AA in a chronic mouse model. MATERIALS AND METHODS: C3H/He mice in the AA group (n = 24/group) were given ad libitum access to distilled water containing 3 µg/mL AA (0.5 mg/kg/day) for 56 days and drinking water from days 57 to 84. The severity of tubulointerstitial injury and fibrosis were evaluated using the tubulointerstitial histological score (TIHS) and Masson's trichrome staining. Urinary and serum methylglyoxal were determined by high-performance liquid chromatography (HPLC); urinary d-lactate were determined by column-switching HPLC. RESULTS: After AA withdrawal, serum methylglyoxal in the AA group increased from day 56 (429.4 ± 48.3 µg/L) to 84 (600.2 ± 99.9 µg/L), and peaked on day 70 (878.3 ± 171.8 µg/L; p < 0.05); TIHS and fibrosis exhibited similar patterns. Urinary methylglyoxal was high on day 56 (3.522 ± 1.061 µg), declined by day 70 (1.583 ± 0.437 µg) and increased by day 84 (2.390 ± 0.130 µg). Moreover, urinary d-lactate was elevated on day 56 (82.10 ± 18.80 µg) and higher from day 70 (201.10 ± 90.82 µg) to 84 (193.28 ± 61.32 µg). CONCLUSIONS: Methylglyoxal is induced after AA-induced tubulointerstitial injury, so methylglyoxal excretion and metabolism may be a detoxification and repair strategy. A low cumulative AA dose is the key factor that limits tubulointerstitial injury and helps to repair. Thus, AA-containing herbs, especially Xixin, should be used at low doses for short durations (less than one month).


Asunto(s)
Ácidos Aristolóquicos/toxicidad , Ácidos Aristolóquicos/uso terapéutico , Medicamentos Herbarios Chinos/toxicidad , Medicamentos Herbarios Chinos/uso terapéutico , Enfermedades Renales/inducido químicamente , Ácido Láctico/análisis , Piruvaldehído/análisis , Animales , Colágeno/metabolismo , Modelos Animales de Enfermedad , Femenino , Fibrosis/inducido químicamente , Fibrosis/patología , Enfermedades Renales/sangre , Enfermedades Renales/patología , Enfermedades Renales/orina , Túbulos Renales/patología , Ácido Láctico/orina , Lactoilglutatión Liasa/metabolismo , Ratones Endogámicos C3H , Piruvaldehído/sangre , Piruvaldehído/orina
2.
Mol Genet Metab ; 122(1-2): 51-59, 2017 09.
Artículo en Inglés | MEDLINE | ID: mdl-28712602

RESUMEN

BACKGROUND: Propionic acidemia is a rare metabolic disorder caused by a deficiency of propionyl- CoA carboxylase, the enzyme converting propionyl-CoA to methylmalonyl-CoA that subsequently enters the citric acid cycle as succinyl-CoA. Patients with propionic acidemia cannot metabolize propionic acid, which combines with oxaloacetate to form methylcitric acid. This, with the defective supply of succinyl-CoA, may lead to a deficiency in citric acid cycle intermediates. PURPOSE: The objective of this study was to determine whether supplements with glutamine (400mg/kg per day), citrate (7.5mEq/kg per day), or ornithine α-ketoglutarate (400mg/kg per day) (anaplerotic agents that could fill up the citric acid cycle) would affect plasma levels of glutamine and ammonia, the urinary excretion of Krebs cycle intermediates, and the clinical outcome in 3 patients with propionic acidemia. METHODS: Each supplement was administered daily for four weeks with a two week washout period between supplements. The supplement that produced the most favorable changes was supplemented for 30 weeks following the initial study period and then for a 2 year extension. RESULTS: The urinary excretion of the Krebs cycle intermediates, α-ketoglutarate, succinate, and fumarate increased significantly compared to baseline during citrate supplementation, but not with the other two supplements. For this reason, citrate supplements were continued in the second part of the study. The urinary excretion of methylcitric acid and 3-hydroxypropionic acid did not change with any intervention. No significant changes in ammonia or glutamine levels were observed with any supplement. However, supplementation with any anaplerotic agents normalized the physiological buffering of ammonia by glutamate, with plasma glutamate and alanine levels significantly increasing, rather than decreasing with increasing ammonia levels. No significant side effects were observed with any therapy and safety labs (blood counts, chemistry and thyroid profile) remained unchanged. Motor and cognitive development was severely delayed before the trial and did not change significantly with therapy. Hospitalizations per year did not change during the trial period, but decreased significantly (p<0.05) in the 2years following the study (when citrate was continued) compared to the 2years before and during the study. CONCLUSIONS: These results indicate that citrate entered the Krebs cycle providing successful anaplerotic therapy by increasing levels of the downstream intermediates of the Krebs cycle: α-ketoglutarate, succinate and fumarate. Citrate supplements were safe and might have contributed to reduce hospitalizations in patients with propionic acidemia.


Asunto(s)
Ciclo del Ácido Cítrico/efectos de los fármacos , Ácido Cítrico/administración & dosificación , Suplementos Dietéticos , Glutamina/administración & dosificación , Ornitina/análogos & derivados , Acidemia Propiónica/dietoterapia , Aminoácidos/sangre , Amoníaco/sangre , Ligasas de Carbono-Carbono/metabolismo , Niño , Preescolar , Citratos/orina , Ácido Cítrico/efectos adversos , Suplementos Dietéticos/efectos adversos , Femenino , Glutamina/efectos adversos , Glutamina/sangre , Humanos , Ácido Láctico/análogos & derivados , Ácido Láctico/orina , Masculino , Ornitina/administración & dosificación , Acidemia Propiónica/metabolismo , Acidemia Propiónica/fisiopatología , Resultado del Tratamiento
3.
J Nutr Biochem ; 34: 136-45, 2016 08.
Artículo en Inglés | MEDLINE | ID: mdl-27309592

RESUMEN

A (1)H NMR global metabolomics approach was used to investigate the urinary metabolome changes in female rats gavaged with partially purified cranberry procyanidins (PPCP) or partially purified apple procyanidins (PPAP). After collecting 24-h baseline urine, 24 female Sprague-Dawley rats were randomly separated into two groups and gavaged with PPCP or PPAP twice using a dose of 250 mg extracts per kilogram body weight. The 24-h urine samples were collected after the gavage. Urine samples were analyzed using (1)H NMR. Multivariate analyses showed that the urinary metabolome in rats was modified after administering PPCP or PPAP compared to baseline urine metabolic profiles. 2D (1)H-(13)C HSQC NMR was conducted to assist identification of discriminant metabolites. An increase of hippurate, lactate and succinate and a decrease of citrate and α-ketoglutarate were observed in rat urine after administering PPCP. Urinary levels of d-glucose, d-maltose, 3-(3'-hydroxyphenyl)-3-hydroxypropanoic acid, p-hydroxyphenylacetic acid, formate and phenol increased but citrate, α-ketoglutarate and creatinine decreased in rats after administering PPAP. Furthermore, the NMR analysis showed that the metabolome in the urine of rats administered with PPCP differed from those gavaged with PPAP. Compared to PPAP, PPCP caused an increase of urinary excretion of hippurate but a decrease of 3-(3'-hydroxyphenyl)-3-hydroxypropanoic acid, p-hydroxyphenylacetic acid and phenol. These metabolome changes caused by cranberry procyanidins may help to explain its reported health benefits and identify biomarkers of cranberry procyanidin intake.


Asunto(s)
Antiinfecciosos Urinarios/administración & dosificación , Suplementos Dietéticos , Frutas/química , Metabolómica/métodos , Extractos Vegetales/administración & dosificación , Proantocianidinas/administración & dosificación , Vaccinium macrocarpon/química , Animales , Antiinfecciosos Urinarios/metabolismo , Biomarcadores/orina , Análisis Discriminante , Femenino , Liofilización , Hipuratos/orina , Ácido Láctico/orina , Espectroscopía de Resonancia Magnética , Malus/química , Extractos Vegetales/química , Extractos Vegetales/metabolismo , Análisis de Componente Principal , Proantocianidinas/análisis , Proantocianidinas/metabolismo , Distribución Aleatoria , Ratas Sprague-Dawley , Eliminación Renal , Ácido Succínico/orina
4.
Mol Genet Metab ; 116(4): 260-8, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26547562

RESUMEN

BACKGROUND: The treatment of long-chain mitochondrial ß-oxidation disorders (LC-FOD) with a low fat-high carbohydrate diet, a diet rich in medium-even-chain triglycerides (MCT), or a combination of both has been associated with high morbidity and mortality for decades. The pathological tableau appears to be caused by energy deficiency resulting from reduced availability of citric acid cycle (CAC) intermediates required for optimal oxidation of acetyl-CoA. This hypothesis was investigated by diet therapy with carnitine and anaplerotic triheptanoin (TH). METHODS: Fifty-two documented LC-FOD patients were studied in this investigation (age range: birth to 51 years). Safety monitoring included serial quantitative measurements of routine blood chemistries, blood levels of carnitine and acylcarnitines, and urinary organic acids. RESULTS: The average frequency of serious clinical complications were reduced from ~60% with conventional diet therapy to 10% with TH and carnitine treatment and mortality decreased from ~65% with conventional diet therapy to 3.8%. Carnitine supplementation was uncomplicated. CONCLUSION: The energy deficiency in LC-FOD patients was corrected safely and more effectively with the triheptanoin diet and carnitine supplement than with conventional diet therapy. Safe intervention in neonates and infants will permit earlier intervention following pre-natal diagnosis or diagnosis by expanded newborn screening.


Asunto(s)
Carnitina/uso terapéutico , Ácidos Grasos/metabolismo , Errores Innatos del Metabolismo Lipídico/dietoterapia , Enfermedades Mitocondriales/dietoterapia , Triglicéridos/uso terapéutico , Administración Oral , Adolescente , Adulto , Carnitina/análogos & derivados , Carnitina/sangre , Niño , Preescolar , Ácido Cítrico/orina , Femenino , Humanos , Lactante , Recién Nacido , Ácido Láctico/orina , Errores Innatos del Metabolismo Lipídico/sangre , Errores Innatos del Metabolismo Lipídico/diagnóstico , Errores Innatos del Metabolismo Lipídico/mortalidad , Malatos/orina , Masculino , Persona de Mediana Edad , Enfermedades Mitocondriales/sangre , Enfermedades Mitocondriales/diagnóstico , Enfermedades Mitocondriales/mortalidad , Oxidación-Reducción , Ácido Succínico/orina , Análisis de Supervivencia , Resultado del Tratamiento
5.
Yao Xue Xue Bao ; 48(11): 1733-7, 2013 Nov.
Artículo en Chino | MEDLINE | ID: mdl-24475714

RESUMEN

To investigate the intervention effects of Morinda officinalis How. on 'Kidney-yang deficiency syndrome' induced by hydrocortisone in rats, the metabolic profiles of rat urine were characterized using proton nuclear magnetic resonance and principal component analysis (PCA) was applied to study the trajectory of urinary metabolic phenotype of rats with 'Kidney-yang deficiency syndrome' under administration of M. officinalis at different time points. Meanwhile, the intervention effects of M. officinalis on urinary metabolic potential biomarkers associated with 'Kidney-yang deficiency syndrome' were also discussed. The experimental results showed that in accordance to the increased time of administration, an obvious tendency was observed that clustering of the treatment group moved gradually closed to that of the control group. Eight potential biomarkers including citrate, succinate, alpha-ketoglutarate, lactate, betaine, sarcosine, alanine and taurine were definitely up- or down-regulated. In conclusion, the effectiveness of M. oficinalis on 'Kidney-yang deficiency syndrome' is proved using the established metabonomic method and the regulated metabolic pathways involve energy metabolism, transmethylation and transportation of amine. Meanwhile, the administration of M. officinalis can alleviate the kidney impairment induced by 'Kidney-yang deficiency syndrome'.


Asunto(s)
Biomarcadores/orina , Medicamentos Herbarios Chinos/farmacología , Enfermedades Renales/orina , Morinda/química , Deficiencia Yang/orina , Alanina/orina , Animales , Betaína/orina , Ácido Cítrico/orina , Medicamentos Herbarios Chinos/aislamiento & purificación , Hidrocortisona , Ácidos Cetoglutáricos/orina , Enfermedades Renales/inducido químicamente , Ácido Láctico/orina , Espectroscopía de Resonancia Magnética , Masculino , Metabolómica/métodos , Plantas Medicinales/química , Análisis de Componente Principal , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Sarcosina/orina , Ácido Succínico/orina , Taurina/orina , Deficiencia Yang/inducido químicamente
6.
J Inherit Metab Dis ; 33 Suppl 3: S315-9, 2010 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20652410

RESUMEN

We present a 32-year-old patient who, from age 7 months, developed photophobia, left-eye ptosis and progressive muscular weakness. At age 7 years, she showed normal psychomotor development, bilateral ptosis and exercise-induced weakness with severe acidosis. Basal blood and urine lactate were normal, increasing dramatically after effort. PDHc deficiency was demonstrated in muscle and fibroblasts without detectable PDHA1 mutations. Ketogenic diet was ineffective, however thiamine gave good response although bilateral ptosis and weakness with acidosis on exercise persisted. Recently, DLD gene analysis revealed a homozygous missense mutation, c.1440 A>G (p.I480M), in the interface domain. Both parents are heterozygous and DLD activity in the patient's fibroblasts is undetectable. The five patients that have been reported with DLD-interface mutations suffered fatal deteriorations. Our patient's disease is milder, only myopathic, more similar to that due to mutation p.G229C in the NAD(+)-binding domain. Two of the five patients presented mutations (p.D479V and p.R482G) very close to the present case (p.I480M). Despite differing degrees of clinical severity, all three had minimal clues to DLD deficiency, with occasional minor increases in α-ketoglutarate and branched-chain amino acids. In the two other patients, hypertrophic cardiomyopathy was a significant feature that has been attributed to moonlighting proteolytic activity of monomeric DLD, which can degrade other mitochondrial proteins, such as frataxin. Our patient does not have cardiomyopathy, suggesting that p.I480M may not affect the DLD ability to dimerize to the same extent as p.D479V and p.R482G. Our patient, with a novel mutation in the DLD interface and mild clinical symptoms, further broadens the spectrum of this enzyme defect.


Asunto(s)
Acidosis Láctica/genética , Enfermedad de la Orina de Jarabe de Arce/genética , Debilidad Muscular/genética , Mutación Missense , Ácido Tióctico/análogos & derivados , Acidosis Láctica/diagnóstico , Acidosis Láctica/tratamiento farmacológico , Acidosis Láctica/enzimología , Acidosis Láctica/fisiopatología , Adulto , Secuencia de Aminoácidos , Secuencia de Bases , Biomarcadores/sangre , Biomarcadores/orina , Blefaroptosis/diagnóstico , Blefaroptosis/enzimología , Blefaroptosis/genética , Células Cultivadas , Análisis Mutacional de ADN , Suplementos Dietéticos , Femenino , Predisposición Genética a la Enfermedad , Herencia , Heterocigoto , Homocigoto , Humanos , Ácido Láctico/sangre , Ácido Láctico/orina , Enfermedad de la Orina de Jarabe de Arce/diagnóstico , Enfermedad de la Orina de Jarabe de Arce/tratamiento farmacológico , Enfermedad de la Orina de Jarabe de Arce/enzimología , Enfermedad de la Orina de Jarabe de Arce/fisiopatología , Datos de Secuencia Molecular , Fuerza Muscular/genética , Debilidad Muscular/diagnóstico , Debilidad Muscular/tratamiento farmacológico , Debilidad Muscular/enzimología , Debilidad Muscular/fisiopatología , Linaje , Fenotipo , Fotofobia/diagnóstico , Fotofobia/enzimología , Fotofobia/genética , Estructura Terciaria de Proteína , Enfermedad por Deficiencia del Complejo Piruvato Deshidrogenasa/diagnóstico , Enfermedad por Deficiencia del Complejo Piruvato Deshidrogenasa/enzimología , Enfermedad por Deficiencia del Complejo Piruvato Deshidrogenasa/genética , España , Tiamina/uso terapéutico , Ácido Tióctico/química , Ácido Tióctico/deficiencia , Ácido Tióctico/genética , Resultado del Tratamiento
7.
Toxicol Appl Pharmacol ; 232(2): 292-301, 2008 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-18706438

RESUMEN

Despite an increasing application of copper nanoparticles, there is a serious lack of information concerning their impact on human health and the environment. In this study, the biochemical compositions of urine, serum, and extracts of liver and kidney tissues of rats treated with nano-copper at the different doses (50, 100, and 200 mg/kg/d for 5 d) were investigated using (1)H NMR techniques with the pattern recognition methods. Serum biochemical analysis and histopathological examinations of the liver and kidney of all the rats were simultaneously performed. All the results indicated that the effects produced by nano-copper at a dose of 100 or 50 mg/kg/d were less than those induced at a higher dose of 200 mg/kg/d. Nano-copper induced overt hepatotoxicity and nephrotoxicity at 200 mg/kg/d for 5 d, which mainly involved scattered dot hepatocytic necrosis and widespread renal proximal tubule necrosis. Increased citrate, succinate, trimethylamine-N-oxide, glucose, and amino acids, accompanied by decreased creatinine levels were observed in the urine; furthermore, elevated levels of lactate, 3-hydroxybutyrate, acetate, creatine, triglycerides, and phosphatide and reduced glucose levels were observed in the serum. The predominant changes identified in the liver tissue aqueous extracts included increased lactate and creatine levels together with reduced glutamine and taurine levels, and the metabolic profile of the kidney tissue aqueous extracts showed an increase in lactate and a drop in glucose. In the chloroform/methanol extracts of the liver and kidney tissues, elevated triglyceride species were identified. These changes suggested that mitochondrial failure, enhanced ketogenesis, fatty acid beta-oxidation, and glycolysis contributed to the hepatotoxicity and nephrotoxicity induced by nano-copper at 200 mg/kg/d for 5 d. An increase in triglycerides in the serum, liver and kidney tissues could serve as a potential sensitive biomarker reflecting the lipidosis induced by nano-copper. The data generated from the current study completely supports the fact that an integrated metabolomic approach is promising for the development of a rapid in vivo screening method for nanotoxicity.


Asunto(s)
Enfermedad Hepática Inducida por Sustancias y Drogas , Cobre/toxicidad , Enfermedades Renales/inducido químicamente , Enfermedades Renales/metabolismo , Hepatopatías/metabolismo , Nanopartículas/toxicidad , Nanotecnología/métodos , Animales , Biomarcadores/sangre , Biomarcadores/metabolismo , Biomarcadores/orina , Glucemia/metabolismo , Cobre/análisis , Evaluación Preclínica de Medicamentos/métodos , Enfermedades Renales/patología , Ácido Láctico/sangre , Ácido Láctico/metabolismo , Ácido Láctico/orina , Hepatopatías/patología , Masculino , Nanopartículas/análisis , Tamaño de la Partícula , Ratas , Ratas Wistar
8.
J Inherit Metab Dis ; 29(2-3): 288-98, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16763890

RESUMEN

In a series of 137 patients with methylmalonic acidaemia (MMA) and propionic acidaemia (PA) diagnosed since the early 1970s, we report in more detail 81 patients (51 MMA and 30 PA) diagnosed between 1988 and 2005. In this series, 14% of patients died at initial access revealing the disease before or despite treatment, 18% died later, and the remainder (68%) are still alive. All patients were treated with the same protocol of enteral feeds with a low-protein diet adjusted to individual tolerance, carnitine, antibiotics, and only occasional use of an amino acid (AA) mixture. There was intensive follow-up and monitoring using measurements of urinary urea. Thirty-nine patients with severe forms, followed for more than 3 years, are analysed in particular detail. Of the 17 PA patients, 6 had moderate disability (all neonatal-onset forms), whereas 11 were normal or slightly delayed in their mental development. Four presented with cardiomyopathy, of whom 2 died. Of the 22 MMA patients, 13 presented in the neonatal period, of whom 3 died later, 2 are in renal failure and only 5 are still alive and have a normal or slightly delayed mental development. In the 9 patients with late-onset forms, there were no deaths and all patients but one have normal mental development. Among the 39 patients, only 40% were given an AA supplement at 3 years, and 50% between 6 and 11 years. The actual intake of natural protein was 0.92, 0.78 and 0.77 g/kg per day at 3, 6 and 11 years, respectively, in patients without AA supplementation, whereas it was 0.75, 0.74 and 0.54 g/kg per day in the group who received small quantities of AA (0.4-0.6 g/kg per day). In both groups, feeding disorders were frequent: 55% at 3 years, 35% at 6 years and 12% at 11 years. Many patients were given a food supplement by tube overnight or were even exclusively tube fed: 60% at 3 years, 48% at 6 years and still 27% at 11 years. Growth velocity was near the normal values. Plasma valine and isoleucine were low to very low, as were leucine and phenylalanine but to a lesser extent. Albumin, vitamins, trace elements and markers of bone metabolism were within the normal values. IGF1, 24-hour urine calcium and body mass density were low. Body composition showed a normal to low lean mass and a normal to high fat mass.


Asunto(s)
Errores Innatos del Metabolismo de los Aminoácidos/terapia , Aminoácidos/uso terapéutico , Dieta con Restricción de Proteínas , Suplementos Dietéticos , Nutrición Enteral , Ácido Metilmalónico/orina , Propionatos/orina , Errores Innatos del Metabolismo de los Aminoácidos/dietoterapia , Errores Innatos del Metabolismo de los Aminoácidos/tratamiento farmacológico , Errores Innatos del Metabolismo de los Aminoácidos/orina , Aminoácidos/sangre , Estatura , Peso Corporal , Química Farmacéutica , Niño , Preescolar , Proteínas en la Dieta/metabolismo , Ingestión de Alimentos , Femenino , Estudios de Seguimiento , Hospitalización , Humanos , Ácido Láctico/análogos & derivados , Ácido Láctico/orina , Masculino , Evaluación Nutricional , Resultado del Tratamiento
9.
Arch Neurol ; 62(2): 317-20, 2005 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-15710863

RESUMEN

BACKGROUND: Primary coenzyme Q(10) (CoQ(10)) deficiency is rare. The encephalomyopathic form, described in few families, is characterized by exercise intolerance, recurrent myoglobinuria, developmental delay, ataxia, and seizures. OBJECTIVE: To report a rare manifestation of CoQ(10) deficiency with isolated mitochondrial myopathy without central nervous system involvement. METHODS: The patient was evaluated for progressive muscle weakness. Comprehensive clinical evaluation and muscle biopsy were performed for histopathologic analysis and mitochondrial DNA and respiratory chain enzyme studies. The patient began taking 150 mg/d of a CoQ(10) supplement. RESULTS: The elevated creatine kinase and lactate levels with abnormal urine organic acid and acylcarnitine profiles in this patient suggested a mitochondrial disorder. Skeletal muscle histochemical evaluation revealed ragged red fibers, and respiratory chain enzyme analyses showed partial reductions in complex I, I + III, and II + III activities with greater than 200% of normal citrate synthase activity. The CoQ(10) concentration in skeletal muscle was 46% of the normal reference mean. The in vitro addition of 50 micromol/L of coenzyme Q(1) to the succinate cytochrome-c reductase assay of the patient's skeletal muscle whole homogenate increased the succinate cytochrome-c reductase activity 8-fold compared with 2.8-fold in the normal control homogenates. Follow-up of the patient in 6 months demonstrated significant clinical improvement with normalization of creatine kinase and lactate levels. CONCLUSIONS: The absence of central nervous system involvement and recurrent myoglobinuria expands the clinical phenotype of this treatable mitochondrial disorder. The complete recovery of myopathy with exogenous CoQ(10) supplementation observed in this patient highlights the importance of early identification and treatment of this genetic disorder.


Asunto(s)
Miopatías Mitocondriales/enzimología , Debilidad Muscular/enzimología , Ubiquinona/análogos & derivados , Ubiquinona/deficiencia , Biopsia/métodos , Niño , Coenzimas , Creatina Quinasa/orina , ADN Mitocondrial/metabolismo , Transporte de Electrón/fisiología , Humanos , Ácido Láctico/orina , Masculino , Microscopía Electrónica de Transmisión/métodos , Mitocondrias Musculares/enzimología , Mitocondrias Musculares/patología , Mitocondrias Musculares/ultraestructura , Miopatías Mitocondriales/dietoterapia , Miopatías Mitocondriales/genética , Miopatías Mitocondriales/fisiopatología , Debilidad Muscular/tratamiento farmacológico , Debilidad Muscular/genética , Debilidad Muscular/fisiopatología , Músculo Esquelético/enzimología , Músculo Esquelético/patología , Músculo Esquelético/fisiopatología , Músculo Esquelético/ultraestructura , Coloración y Etiquetado/métodos , Ubiquinona/administración & dosificación
10.
Analyst ; 129(6): 535-41, 2004 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-15152332

RESUMEN

The effects of the administration of a single dose of the model nephrotoxin mercuric chloride (2.0 mg kg(-1), subcutaneous) to male Wistar-derived rats on the urinary metabolite profiles of a range of endogenous metabolites has been investigated using (1)H NMR and HPLC-MS. Urine samples were collected daily for 9 days from both dosed and control animals. Analysis of these samples revealed marked changes in the pattern of endogenous metabolites as a result of HgCl(2) toxicity. Peak disturbances in the urinary metabolite profiles were observed (using both NMR and HPLC-MS) at 3 days post dose. Thereafter the urinary metabolite profile gradually returned to a more normal composition. Markers of toxicity identified by (1)H NMR spectroscopy were raised concentrations of lactate, alanine, acetate, succinate, trimethylamine (TMA), and glucose. Reductions in the urinary excretion of citrate and alpha-ketoglutarate were also seen. Markers identified by HPLC-MS, in positive ion mode, were kynurenic acid, xanthurenic acid, pantothenic acid and 7-methylguanine which decreased after dosing. In addition an ion at m/z 188, probably 3-amino-2-naphthoic acid, was observed to increase after dosing. As well as these identified compounds other ions at m/z 297 and 267 decreased after dosing. In negative ion mode a range of sulfated compounds were observed, including phenol sulfate and benzene diol sulfate, which decreased after dosing. As well as the sulfated components an unidentified glucuronide at m/z 326 was also observed to decrease after dosing. The results of this study demonstrate the complementary nature of the NMR and MS-based techniques for metabonomic analysis.


Asunto(s)
Riñón/efectos de los fármacos , Ácido Láctico/orina , Cloruro de Mercurio/toxicidad , Acetatos/orina , Alanina/orina , Animales , Biomarcadores/orina , Cromatografía Líquida de Alta Presión , Ácido Cítrico/orina , Glucosa/análisis , Ácidos Cetoglutáricos/orina , Riñón/metabolismo , Espectroscopía de Resonancia Magnética , Masculino , Cloruro de Mercurio/metabolismo , Metilaminas/orina , Ratas , Ratas Wistar , Ácido Succínico/orina
11.
J Nutr ; 132(7): 1945-50, 2002 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-12097674

RESUMEN

Recent clinical studies have provided evidence that marginal biotin deficiency is more common than previously thought. The validity of that conclusion rests on two indicators of biotin status that depend on renal function. Our goal was to develop and assess the usefulness of two additional indicators in detecting marginal biotin status in a rat model, i.e., 1) activity of the biotin-dependent enzyme propionyl-CoA carboxylase in lymphocytes; and 2) urinary excretion of 3-hydroxypropionic acid, an organic acid that reflects decreased activity of propionyl-CoA carboxylase. Marginal-to-moderate biotin deficiency was induced experimentally by an egg-white diet (deficient rats); the biotin-supplemented rats were fed the egg-white diet plus supplemental biotin. Propionyl-CoA carboxylase activity was determined by an optimized H(14)CO(3)(-) incorporation assay. Urinary organic acids were determined by gas chromatography/mass spectrometry. Lymphocyte propionyl-CoA carboxylase activity decreased dramatically and in parallel with hepatic propionyl-CoA carboxylase activity. By d 7, lymphocyte propionyl-CoA carboxylase activity in each rat in the deficient group had decreased to less than the lowest value of any rat on d 0. By two-way ANOVA, the effects of diet (P < 0.0001), time (P < 0.005) and their interaction (P < 0.0001) were all significant. The urinary excretion of 3-hydroxypropionic acid did not differ between the two groups. Lymphocyte propionyl-CoA carboxylase activity is an early and sensitive indicator of marginal biotin deficiency, whereas the urinary excretion of 3-hydroxypropionic acid is not.


Asunto(s)
Biotina/deficiencia , Carboxiliasas/metabolismo , Ácido Láctico/análogos & derivados , Ácido Láctico/orina , Linfocitos/enzimología , Animales , Biomarcadores , Enfermedades Carenciales/diagnóstico , Glicina/análogos & derivados , Glicina/orina , Masculino , Metilmalonil-CoA Descarboxilasa , Ratas , Ratas Sprague-Dawley , Sensibilidad y Especificidad
12.
J Clin Invest ; 109(12): 1617-23, 2002 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-12070309

RESUMEN

We describe a 3-year-old boy with biotin dependency not caused by biotinidase, holocarboxylase synthetase, or nutritional biotin deficiency. We sought to define the mechanism of his biotin dependency. The child became acutely encephalopathic at age 18 months. Urinary organic acids indicated deficiency of several biotin-dependent carboxylases. Symptoms improved rapidly following biotin supplementation. Serum biotinidase activity and Biotinidase gene sequence were normal. Activities of biotin-dependent carboxylases in PBMCs and cultured skin fibroblasts were normal, excluding biotin holocarboxylase synthetase deficiency. Despite extracellular biotin sufficiency, biotin withdrawal caused recurrent abnormal organic aciduria, indicating intracellular biotin deficiency. Biotin uptake rates into fresh PBMCs from the child and into his PBMCs transformed with Epstein Barr virus were about 10% of normal fresh and transformed control cells, respectively. For fresh and transformed PBMCs from his parents, biotin uptake rates were consistent with heterozygosity for an autosomal recessive genetic defect. Increased biotin breakdown was ruled out, as were artifacts of biotin supplementation and generalized defects in membrane permeability for biotin. These results provide evidence for a novel genetic defect in biotin transport. This child is the first known with this defect, which should now be included in the identified causes of biotin dependency.


Asunto(s)
Biotina/deficiencia , Ácido Láctico/análogos & derivados , Simportadores/deficiencia , Amidohidrolasas/metabolismo , Transporte Biológico , Biotinidasa , Ligasas de Carbono-Carbono/metabolismo , Carboxiliasas/metabolismo , Proteínas Portadoras/genética , Línea Celular Transformada , Preescolar , Femenino , Humanos , Ácido Láctico/orina , Leucocitos Mononucleares/citología , Masculino , Glicoproteínas de Membrana/genética , Metilmalonil-CoA Descarboxilasa , Piruvato Carboxilasa/metabolismo , Síndrome de Abstinencia a Sustancias/enzimología , Síndrome de Abstinencia a Sustancias/genética , Síndrome de Abstinencia a Sustancias/orina , Valeratos/orina
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