RESUMEN
BACKGROUND: Gastroesophageal reflux and Barrett's esophagus are significant risk factors for the development of esophageal adenocarcinoma. Group IIa secretory phospholipase A2 (sPLA2) catalyzes the production of various proinflammatory metabolites and plays a critical role in promoting reflux-induced inflammatory changes within the distal esophagus. We hypothesized that inhibition of sPLA2 in human Barrett's cells would attenuate adhesion molecule expression via decreased activation of nuclear factor kappa B (NF-κB) and decrease cell proliferation, possibly mitigating the invasive potential of Barrett's esophagus. MATERIALS AND METHODS: Normal human esophageal epithelial cells (HET1A) and Barrett's cells (CPB) were assayed for baseline sPLA2 expression. CPB cells were treated with a specific inhibitor of sPLA2 followed by tumor necrosis factor-α. Protein expression was evaluated using immunoblotting. Cell proliferation was assessed using an MTS cell proliferation assay kit. Statistical analysis was performed using the Student's t-test or analysis of variance, where appropriate. RESULTS: CPB cells demonstrated higher baseline sPLA2 expression than HET1A cells (P = 0.0005). Treatment with 30 µM sPLA2 inhibitor significantly attenuated intercellular adhesion molecule-1 (P = 0.004) and vascular cell adhesion molecule-1 (P < 0.0001) expression as well as decreased NF-κB activation (P = 0.002). sPLA2 inhibition decreased cell proliferation in a dose-dependent manner (P < 0.001 for 15, 20, and 30 µM doses). CONCLUSIONS: sPLA2 inhibition in human Barrett's cells decreases cellular adhesive properties and NF-κB activation as well as decreases cell proliferation, signifying downregulation of the inflammatory response and possible attenuation of cellular malignant potential. These findings identify sPLA2 inhibition as a potential chemopreventive target for premalignant lesions of the esophagus.
Asunto(s)
Esófago de Barrett/patología , Esófago/patología , Fosfolipasas A2 Grupo II/antagonistas & inhibidores , Ácidos Pentanoicos/farmacología , Adenocarcinoma/patología , Adenocarcinoma/prevención & control , Esófago de Barrett/tratamiento farmacológico , Adhesión Celular/efectos de los fármacos , Línea Celular , Proliferación Celular/efectos de los fármacos , Evaluación Preclínica de Medicamentos , Neoplasias Esofágicas/patología , Neoplasias Esofágicas/prevención & control , Esófago/citología , Fosfolipasas A2 Grupo II/metabolismo , Humanos , Ácidos Pentanoicos/uso terapéuticoRESUMEN
Alcoholic hepatitis is an acute, inflammatory liver disease associated with high morbidity and mortality both in the short term and long term. Alcoholic hepatitis often arises in patients with a background of chronic liver disease and it is characterised by the rapid onset of jaundice and the development of myriad complications. Medical therapy for severe alcoholic hepatitis relies on corticosteroids, which have modest effectiveness. Abstinence from alcohol is critically important in patients with alcoholic hepatitis, but recidivism is high. Because of the absence of effective medical treatments for alcoholic hepatitis and alcohol dependency, there is a pressing need to develop new and effective therapeutics. Supported by promising preliminary and preclinical studies, many ongoing clinical trials of new therapies for alcoholic hepatitis are currently underway and are discussed further in this Series paper.
Asunto(s)
Corticoesteroides/uso terapéutico , Alcoholismo/terapia , Antiinflamatorios/uso terapéutico , Hepatitis Alcohólica/terapia , Abstinencia de Alcohol , Alcoholismo/complicaciones , Antibacterianos/uso terapéutico , Antioxidantes/uso terapéutico , Suplementos Dietéticos , Hepatitis Alcohólica/diagnóstico , Hepatitis Alcohólica/etiología , Humanos , Ácidos Pentanoicos/uso terapéutico , Probióticos/uso terapéutico , Receptores de Interleucina-1/antagonistas & inhibidores , Transducción de Señal , Factor de Necrosis Tumoral alfa/antagonistas & inhibidoresRESUMEN
Psoriasis is a common inflammatory skin disorder that is characterized by keratinocyte hyperproliferation and abnormal differentiation, resulting in the thickening of the epidermis and stratum corneum. In this study, we investigated in vitro and in vivo pharmacological effects of tussilagonone (TGN), a sesquiterpenoid isolated from Tussilago farfara, on transcription factors relevant for the pathogenesis of psoriasis. TGN inhibited activation of NF-κB and STAT3, leading to the attenuated expression of psoriasis-related inflammatory genes and suppression of keratinocyte hyperproliferation. Mechanistically, we show that the inhibition of NF-κB and STAT3 by TGN is mediated through activation of the cytoprotective transcription factor NRF2. Evaluation of in vivo antipsoriatic effects of topical TGN in the imiquimod-induced psoriasis-like dermatitis mouse model demonstrated amelioration of imiquimod-induced phenotypical changes, lesion severity score, epidermal thickening, and reduction in dermal cellularity. The spleen index also diminished in TGN-treated mice, suggesting anti-inflammatory properties of TGN. Moreover, TGN significantly attenuated the imiquimod-induced mRNA levels of psoriasis-associated inflammatory cytokines and antimicrobial peptides and reduced epidermal hyperproliferation. Taken together, TGN, as a potent NRF2 activator, is a promising therapeutic candidate for the development of antipsoriatic agents derived from medicinal plants.
Asunto(s)
Antiinflamatorios/farmacología , Factor 2 Relacionado con NF-E2/agonistas , Ácidos Pentanoicos/farmacología , Psoriasis/tratamiento farmacológico , Sesquiterpenos/farmacología , Administración Cutánea , Adulto , Animales , Antiinflamatorios/uso terapéutico , Línea Celular , Proliferación Celular/efectos de los fármacos , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Femenino , Humanos , Imiquimod/toxicidad , Queratinocitos/efectos de los fármacos , Queratinocitos/patología , Ratones , Factor 2 Relacionado con NF-E2/metabolismo , Ácidos Pentanoicos/uso terapéutico , Psoriasis/inducido químicamente , Psoriasis/inmunología , Psoriasis/patología , Sesquiterpenos/uso terapéutico , Tussilago/químicaRESUMEN
BACKGROUND & AIMS: Hepatocyte apoptosis, the hallmark of non-alcoholic steatohepatitis (NASH) contributes to liver injury and fibrosis. Although, both the intrinsic and extrinsic apoptotic pathways are involved in the pathogenesis of NASH, the final common step of apoptosis is executed by a family of cysteine-proteases termed caspases. Thus, our aim was to ascertain if administration of Emricasan, a pan-caspase inhibitor, ameliorates liver injury and fibrosis in a murine model of NASH. METHODS: C57/BL6J-mice were fed regular chow or high fat diet (HFD) for 20 weeks. All mice were treated with vehicle or Emricasan. RESULTS: Mice fed a HFD diet demonstrate a five-fold increase in hepatocyte apoptosis by the TUNEL assay and a 1.5-fold and 1.3-fold increase in caspase-3 and-8 activities respectively; this increase in apoptosis was substantially attenuated in mice fed a HFD treated with Emricasan (HFD-Em). Likewise, liver injury and inflammation were reduced in mice fed HFD-Em as compare to HFD by measuring serum aspartate aminotransferase and alanine aminotransferase levels, NAS histological score and IL 1-ß, TNF-α, monocyte chemoattractant protein (MCP-1) and C-X-C chemokine ligand-2 (CXCL2) quantitative reverse-transcription polymerase chain reaction (qPCR). These differences could not be attributed to differences in hepatic steatosis as liver triglycerides content were similar in both HFD groups. Hepatic fibrosis was reduced by Emricasan in HFD animals by decreasing αSMA (a marker for hepatic stellate cell activation), fibrosis score, Sirius red staining, hydroxyproline liver content and profibrogenic cytokines by qPCR. CONCLUSION: In conclusion, these data demonstrate that in a murine model of NASH, liver injury and fibrosis are suppressed by inhibiting hepatocytes apoptosis and suggests that Emricasan may be an attractive antifibrotic therapy in NASH.
Asunto(s)
Apoptosis/efectos de los fármacos , Inhibidores de Caspasas/uso terapéutico , Hepatocitos/efectos de los fármacos , Enfermedad del Hígado Graso no Alcohólico/prevención & control , Ácidos Pentanoicos/uso terapéutico , Animales , Inhibidores de Caspasas/farmacología , Dieta Alta en Grasa/efectos adversos , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Fibrosis , Hepatitis/prevención & control , Hígado/efectos de los fármacos , Hígado/patología , Masculino , Ratones Endogámicos C57BL , Enfermedad del Hígado Graso no Alcohólico/etiología , Enfermedad del Hígado Graso no Alcohólico/patología , Ácidos Pentanoicos/farmacologíaRESUMEN
Transient receptor potential vanilloid 1 (TRPV1) is a Ca(2+)-permeable non-selective cation channel that transmits pain signals. TRPV1 is activated by multiple stimuli such as capsaicin, acid, and heat. During inflammation, TRPV1 is reported to be sensitized by protein kinase C (PKC) in dorsal root ganglia (DRG) neurons, which leads to reduction in the threshold of the temperature for TRPV1 activation to body temperature. This sensitization is considered to contribute to chronic inflammatory pain. In a previous study, we discovered orally active 5,5-diarylpentadienamide TRPV1 antagonists. To examine the effects of our TRPV1 antagonists on PKC-sensitized TRPV1, we developed an in vitro assay system to monitor the TRPV1 sensitization by PKC. In this assay system, our TRPV1 antagonists, such as (2E,4Z)-N-[(3R)-3-hydroxy-2-oxo-1,2,3,4-tetrahydro-5-quinolyl]-5-(4-isopropoxyphenyl)-5-(4-trifluoromethylphenyl)-2,4-pentadienamide (K-685), inhibited the activation of TRPV1 sensitized by PKC. The potentiation of heat-induced inward currents by PKC was seen in rat DRG neurons, and K-685 attenuated these currents. Furthermore, K-685 reversed the thermal hyperalgesia and mechanical allodynia in a rat complete Freund's adjuvant-induced inflammatory pain model. These results therefore suggest that K-685 has a strong potential as a new analgesic drug for the treatment of inflammatory pain.
Asunto(s)
Analgésicos , Dolor Crónico/tratamiento farmacológico , Dolor Crónico/genética , Adyuvante de Freund/efectos adversos , Inflamación/complicaciones , Ácidos Pentanoicos/farmacología , Ácidos Pentanoicos/uso terapéutico , Proteína Quinasa C/fisiología , Quinolonas/farmacología , Quinolonas/uso terapéutico , Canales Catiónicos TRPV/antagonistas & inhibidores , Canales Catiónicos TRPV/metabolismo , Animales , Dolor Crónico/etiología , Modelos Animales de Enfermedad , Masculino , Ratas , Ratas Sprague-DawleyRESUMEN
In this study, the ability of the chelating agent monensic acid (administered as the tetraethylammonium salt) to reduce the cadmium (Cd) concentration in the kidneys, liver, heart, lungs, spleen and testes of Cd-intoxicated mice was investigated. Chelation therapy with the tetraethylammonium salt of monensic acid led to a significant decrease of the Cd concentration in all of the organs of the Cd-treated mice. This effect varied from 50% in the kidneys to 90% in the hearts of the sacrificed animals (compared to the Cd-treated controls). No redistribution of the toxic metal ions to the brain of the animals as a result of the detoxification with the chelating agent was observed. The detoxification of the animals with the antibiotic salt did not perturb the endogenous levels of copper (Cu) or zinc (Zn). The tetraethylammonium salt of monensic acid significantly ameliorated the Cd-induced total iron (Fe) depletion in the liver and spleen of Cd-treated mice. It also restored to control levels the values of transferrin-bound Fe and the total iron binding capacity (TIBC) of the plasma. These results imply that the tetraethylammonium salt of monensic acid could be an efficient antidote in cases of Cd-intoxication.
Asunto(s)
Cadmio/toxicidad , Furanos/uso terapéutico , Ácidos Pentanoicos/uso terapéutico , Tetraetilamonio/uso terapéutico , Animales , Intoxicación por Cadmio/tratamiento farmacológico , Masculino , Ratones , Ratones Endogámicos ICRRESUMEN
BACKGROUND: Nicobrevin is a proprietary product marketed as an aid to smoking cessation. It contains quinine, menthyl valerate, camphor and eucalyptus oil. OBJECTIVES: The objective of this review was to assess the effects of Nicobrevin on long term smoking cessation SEARCH STRATEGY: We searched the Cochrane Tobacco Addiction Group trials register. SELECTION CRITERIA: Randomized trials comparing Nicobrevin to placebo or an alternative therapeutic control, which reported smoking cessation with at least six months follow up. DATA COLLECTION AND ANALYSIS: Data were sought on the outcome, method of randomization, and completeness of follow up. MAIN RESULTS: We identified no trials meeting the full inclusion criteria including long-term follow up. AUTHORS' CONCLUSIONS: There is no evidence available from long-term trials that Nicobrevin can aid smoking cessation.
Asunto(s)
Ácidos Pentanoicos/uso terapéutico , Quinina/uso terapéutico , Cese del Hábito de Fumar , Prevención del Hábito de Fumar , Terpenos/uso terapéutico , HumanosRESUMEN
OBJECTIVE: To evaluate the effect of Ridogrel enemas (Janssen Research Foundation, Beerse, Belgium) on disease activity and mucosal inflammatory mediators in patients with active left-sided ulcerative colitis. DESIGN AND METHODS: Eleven patients with active left-sided ulcerative colitis were evaluated in an open non-placebo-controlled pilot study. All patients were treated with Ridogrel enemas (300 mg/40 ml once daily) over four weeks. A disease activity score based on clinical, endoscopic and histological criteria was obtained before and after treatment with Ridogrel. The concentrations of thromboxane B2 (TxB2), prostaglandin E2 (PGE2), interleukin-6 (IL-6) and tumour necrosis factor alpha (TNF-alpha) were measured in mucosal biopsies before and after treatment. RESULTS: One patient discontinued treatment because of progression of disease, the other ten patients tolerated the Ridogrel enemas well. Mucosal TxB2 concentration decreased significantly in all patients. The mucosal concentrations of the other inflammatory mediators (PGE2, IL-6 and TNF-alpha) were unaltered. The disease score decreased in five patients. However, clinical improvement was not always associated with a decrease in endoscopic and/or histological scores. CONCLUSIONS: This pilot study shows that Ridogrel enemas selectively reduce mucosal TxB2 concentration.
Asunto(s)
Colitis Ulcerosa/tratamiento farmacológico , Enema , Ácidos Pentanoicos/uso terapéutico , Piridinas/uso terapéutico , Tromboxano-A Sintasa/antagonistas & inhibidores , Adulto , Anciano , Colitis Ulcerosa/patología , Dinoprostona/análisis , Femenino , Mucosa Gástrica/química , Mucosa Gástrica/patología , Humanos , Interleucina-6/análisis , Masculino , Persona de Mediana Edad , Ácidos Pentanoicos/administración & dosificación , Proyectos Piloto , Piridinas/administración & dosificación , Factor de Necrosis Tumoral alfa/análisisRESUMEN
Previous work suggests that cod liver oil helps to protect the microcirculation from the consequence of thromboembolic events. The possibility that altered synthesis of thromboxane A2 accounts for the protective effects seen with cod liver oil was investigated in the present study. This was done using the combined thromboxane A2 synthetase inhibitor and thromboxane A2-prostaglandin H2 receptor blocker R68070 (Ridogrel). A standardized microvascular injury was inflicted on the right iliac artery of the rat to generate emboli. The downstream cremaster muscle was used to visualize the passage of the ensuing emboli and to assess the effects of this arterial injury on capillary perfusion and arteriole diameters. The number of visible emboli was not changed by either cod liver oil diet or Ridogrel administration. However, capillary perfusion was preserved by using cod liver oil (n = 7) and was significantly increased by using Ridogrel (n = 7) in comparison with untreated controls (n = 7) in which capillary perfusion was decreased because of the emboli. The administration of Ridogrel to cod liver oil-treated animals (n = 7) provided no additive benefit. The percentage change in A-2 vessel diameters in cod liver oil-treated (n = 7) animals was no different from the control group (n = 7). Ridogrel (n = 7), on the other hand, produced a significant increase in A-3 vessel (n = 21) diameters, but its effects were comparatively less in the cod liver oil-treated animals (n = 7). The formation of platelet aggregates (emboli) appears relatively independent of thromboxane A2 in the rat. Ridogrel is very effective in protecting the microcirculation, and these effects appear to be mediated by A-3 vasodilatation, which, therefore, is at least partially thromboxane A2-dependent. The positive effects of cod liver oil may be mediated by a mechanism that reduces thromboxane A2 synthesis, but further studies are necessary.
Asunto(s)
Aceite de Hígado de Bacalao/uso terapéutico , Embolia/prevención & control , Inhibidores Enzimáticos/uso terapéutico , Músculo Esquelético/irrigación sanguínea , Ácidos Pentanoicos/uso terapéutico , Piridinas/uso terapéutico , Tromboxano A2/fisiología , Tromboxano-A Sintasa/antagonistas & inhibidores , Animales , Arteriolas/efectos de los fármacos , Embolia/etiología , Embolia/fisiopatología , Arteria Ilíaca/lesiones , Masculino , Microcirculación/efectos de los fármacos , Microcirculación/fisiología , Ratas , Ratas Sprague-Dawley , Tromboxano A2/antagonistas & inhibidoresAsunto(s)
Ácidos Pentanoicos/uso terapéutico , Quinina/uso terapéutico , Prevención del Hábito de Fumar , Terpenos/uso terapéutico , Valeratos/uso terapéutico , Adulto , Ensayos Clínicos como Asunto , Método Doble Ciego , Femenino , Humanos , Masculino , Ácidos Pentanoicos/administración & dosificación , Estudios Prospectivos , Quinina/administración & dosificación , Terpenos/administración & dosificaciónRESUMEN
These studies examine the effects of a hypercholesterolemic diet (with butter and cholesterol added), or a hypertriglyceridemic diet (30% sucrose (w/v) in drinking water), on murine plasma lipids and lipoproteins prepared either by sequential ultracentrifugation or gel exclusion chromatography. The hypercholesterolemic diet increased plasma cholesterol (186%), particularly that associated with very low (VLDL) and low (LDL) density lipoproteins. In addition, the cholesterol/triglyceride ratio in the VLDL fraction rose significantly from 0.10 to 4.0. The hypertriglyceridemic diet raised markedly plasma triglyceride levels (46%) by expanding the circulating VLDL pool (+39%). These dietary modifications were used to provide a model for the examination of 3 classical hypolipidemic drugs (fenofibrate, gemfibrozil and nicotinic acid). In animals receiving the standard diet, fenofibrate, gemfibrozil and nicotinic acid decreased the triglyceride (TG) level (-28%, -31%, and -38%) by lowering VLDL-TG (-37%, -42%, and -49%), fenofibrate and gemfibrozil increased HDL-cholesterol by 18% and 31%, respectively. In animals receiving the hypercholesterolemic diet fenofibrate lowered the total plasma cholesterol level by 40%, at the same time increasing HDL-cholesterol by 23%. In animals fed sucrose, on the other hand, fenofibrate (100 mg/kg per day) and nicotinic acid (900 and 600 mg/kg per day) reduced plasma triglyceride levels (-20%, -40% and -33%), and nicotinic acid (900 mg/kg per day) decreased VLDL-TG by 58%. These results are in good agreement with clinical data from studies in man and suggest that this animal model may provide a useful and rapid screening system for testing new lipid lowering drugs.
Asunto(s)
Hiperlipidemias/sangre , Hipolipemiantes/farmacología , Lipoproteínas/sangre , Animales , Colesterol en la Dieta/administración & dosificación , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Femenino , Fenofibrato/farmacología , Fenofibrato/uso terapéutico , Gemfibrozilo , Hiperlipidemias/etiología , Hipolipemiantes/uso terapéutico , Ratones , Ratones Endogámicos , Niacina/farmacología , Niacina/uso terapéutico , Ácidos Pentanoicos/farmacología , Ácidos Pentanoicos/uso terapéutico , Triglicéridos/sangreAsunto(s)
Hiperlipoproteinemias/tratamiento farmacológico , Hipolipemiantes/uso terapéutico , Resina de Colestiramina/uso terapéutico , Clofibrato/uso terapéutico , Colestipol/uso terapéutico , Dextrotiroxina/uso terapéutico , Femenino , Aceites de Pescado/uso terapéutico , Gemfibrozilo , Humanos , Inhibidores de Hidroximetilglutaril-CoA Reductasas , Masculino , Neomicina/uso terapéutico , Niacina/uso terapéutico , Ácidos Pentanoicos/uso terapéutico , Probucol/uso terapéuticoAsunto(s)
Hiperlipidemias/terapia , Lipoproteínas/sangre , Colesterol/sangre , Clofibrato/uso terapéutico , Enfermedad Coronaria/sangre , Enfermedad Coronaria/complicaciones , Femenino , Gemfibrozilo , Humanos , Hipercolesterolemia/dietoterapia , Hipercolesterolemia/tratamiento farmacológico , Hipercolesterolemia/etiología , Hiperlipoproteinemia Tipo IV/dietoterapia , Hiperlipoproteinemia Tipo IV/etiología , Hiperlipoproteinemias/tratamiento farmacológico , Lactancia , Lovastatina , Naftalenos/uso terapéutico , Niacina/uso terapéutico , Ácidos Pentanoicos/uso terapéutico , Embarazo , Complicaciones del Embarazo/sangre , Complicaciones del Embarazo/dietoterapia , Probucol/uso terapéutico , Enfermedad de Tangier/sangre , Triglicéridos/sangreRESUMEN
The results of the nutritional intervention on one of the twenty MRFIT centers is presented. The reduction in cholesterol was 5.78% after four years. Those results were compared with the changes found in serum cholesterol with a similar diet and the administration of either clofibrate or gemfibrozil. The reduction with clofibrate remained at 5%. However with gemfibrozil the reduction in cholesterol was 16%. The HDL level rose in half of these patients treated with either clofibrate or gemfibrozil. The results without concurrent dietary restriction usually show a greater reduction in VLDL and a greater rise in HDL than we found when nutritional and pharmacologic intervention were combined.