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1.
J Neuroinflammation ; 13(1): 89, 2016 Apr 23.
Artículo en Inglés | MEDLINE | ID: mdl-27107718

RESUMEN

BACKGROUND: Müller glial cells are important regulators of physiological function of retina. In a model disease of retinal inflammation and spontaneous recurrent uveitis in horses (ERU), we could show that retinal Müller glial cells significantly change potassium and water channel protein expression during autoimmune pathogenesis. The most significantly changed channel protein in neuroinflammatory ERU was aquaporin 11 (AQP11). Aquaporins (AQP, 13 members) are important regulators of water and small solute transport through membranes. AQP11 is an unorthodox member of this family and was assigned to a third group of AQPs because of its difference in amino acid sequence (conserved sequence is only 11 %) and especially its largely unknown function. METHODS: In order to gain insight into the distribution, localization, and function of AQP11 in the retina, we first developed a novel monoclonal antibody for AQP11 enabling quantification, localization, and functional studies. RESULTS: In the horse retina, AQP11 was exclusively expressed at Müller glial cell membranes. In uveitic condition, AQP11 disappeared from gliotic Müller cells concomitant with glutamine synthase. Since function of AQP11 is still under debate, we assessed the impact of AQP11 channel on cell volume regulation of primary Müller glial cells under different osmotic conditions. We conclude a concomitant role for AQP11 with AQP4 in water efflux from these glial cells, which is disturbed in ERU. This could probably contribute to swelling and subsequent severe complication of retinal edema through impaired intracellular fluid regulation. CONCLUSIONS: Therefore, AQP11 is important for physiological Müller glia function and the expression pattern and function of this water channel seems to have distinct functions in central nervous system. The significant reduction in neuroinflammation points to a crucial role in pathogenesis of autoimmune uveitis.


Asunto(s)
Acuaporinas/metabolismo , Enfermedades Autoinmunes/veterinaria , Células Ependimogliales/metabolismo , Gliosis/veterinaria , Uveítis/veterinaria , Animales , Acuaporinas/inmunología , Enfermedades Autoinmunes/metabolismo , Enfermedades Autoinmunes/patología , Western Blotting , Gliosis/inmunología , Gliosis/metabolismo , Enfermedades de los Caballos , Caballos , Inmunohistoquímica , Presión Osmótica , Uveítis/metabolismo , Uveítis/patología
2.
J Exp Biol ; 211(Pt 7): 1114-9, 2008 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-18344486

RESUMEN

Survival of freezing not only requires organisms to tolerate ice formation within their body, but also depends on the rapid redistribution of water and cryoprotective compounds between intra- and extracellular compartments. Aquaporins are transmembrane proteins that serve as the major pathway through which water and small uncharged solutes (e.g. glycerol) enter and leave the cell. Consequently, we examined freeze-tolerant larvae of the goldenrod gall fly, Eurosta solidaginis, to determine whether aquaporins are present and if their presence promotes freeze tolerance of specific tissues. Immunoblotting with mammalian anti-AQP2, -AQP3 and -AQP4 revealed corresponding aquaporin homologues in E. solidaginis, whose patterns of expression varied depending on acclimation temperature and desiccation treatment. To examine the role of aquaporins in freeze tolerance, we froze fat body, midgut and salivary gland tissues in the presence and absence of mercuric chloride, an aquaporin inhibitor. Survival of fat body and midgut cells was significantly reduced when mercuric chloride was present. In contrast, survival of the salivary gland did not decrease when it was frozen with mercuric chloride. Overall, this study supports our hypothesis that naturally occurring aquaporins in E. solidaginis are regulated during desiccation and promote cell survival during freezing.


Asunto(s)
Adaptación Fisiológica , Acuaporinas/metabolismo , Deshidratación/metabolismo , Desecación , Congelación , Solidago/parasitología , Tephritidae/fisiología , Adaptación Fisiológica/efectos de los fármacos , Animales , Acuaporinas/antagonistas & inhibidores , Acuaporinas/inmunología , Compuestos Azo , Extractos Celulares , Cuerpo Adiposo/citología , Cuerpo Adiposo/efectos de los fármacos , Tracto Gastrointestinal/citología , Tracto Gastrointestinal/efectos de los fármacos , Larva/efectos de los fármacos , Larva/fisiología , Cloruro de Mercurio/farmacología , Microscopía Fluorescente , Glándulas Salivales/citología , Glándulas Salivales/efectos de los fármacos , Solidago/efectos de los fármacos , Tephritidae/efectos de los fármacos , Supervivencia Tisular/efectos de los fármacos
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