RESUMEN
Asini Corii Collas (ACC; donkey glue) is a crude drug used to promote hematopoiesis and arrest bleeding. Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. Two strategies for extracting DNA from ACC were compared, and the ion-exchange resin procedure was revealed to be more suitable than the silica-based one. Using DNA extracted from ACC by the ion-exchange resin procedure, PCR methods for species-specific detection of donkey, horse, cattle, and pig substances were established. When these species-specific PCR methods were applied to ACC, amplicons were obtained only by the donkey-specific PCR. Cattle-specific PCR detected as little as 0.1% admixture of cattle glue in the ACC. These results suggest that the species-specific PCR methods established in this study would be useful for simple and easy detection of adulteration of ACC.
Asunto(s)
Adhesivos , Citocromos b/genética , ADN/análisis , Equidae/genética , Especificidad de la Especie , Extractos de Tejidos/genética , Adhesivos/normas , Animales , Bovinos , Cromatografía por Intercambio Iónico , Código de Barras del ADN Taxonómico , Contaminación de Medicamentos , Equidae/metabolismo , Caballos , Reacción en Cadena de la Polimerasa/métodos , Control de Calidad , Porcinos , Extractos de Tejidos/metabolismo , Extractos de Tejidos/normasRESUMEN
Toxico-hygienic studies have been carried out of a new polymeric glue to be used in the shoe-making industry. As a result of studies the use of the glue is recommended in the shoe-making industry, conditions of its safe use and the way to maintain current sanitary supervision have been suggested.