RESUMEN
Herein, the isolation of secondary metabolites from the aerial parts of Justicia aequilabris guided by HPLC-MSn and molecular networking analyses is reported. Twenty-two known compounds were dereplicated. Three new lignans (aequilabrines A-C (1-3)) and three known compounds (lariciresinol-4'-O-ß-glucose (4), roseoside (5), and allantoin (6)) were obtained. The anti-inflammatory activity of compounds 1-3 was evaluated in vitro by inhibiting the nitric oxide production (NO) and pro-inflammatory activity on the cytokine IL-1ß. Compounds 2 and 3 showed significant inhibitory activity against NO production, with IC50 values of 9.1 and 7.3 µM, respectively. The maximum inhibition of IL-1ß production was 23.5% (1), 27.3% (2), and 32.5% (3).
Asunto(s)
Antiinflamatorios , Género Justicia , Lignanos , Alantoína/química , Alantoína/aislamiento & purificación , Alantoína/farmacología , Antiinflamatorios/química , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/farmacología , Furanos/química , Furanos/aislamiento & purificación , Furanos/farmacología , Lignanos/química , Lignanos/aislamiento & purificación , Lignanos/farmacología , Estructura Molecular , Óxido Nítrico/antagonistas & inhibidores , Extractos Vegetales/químicaRESUMEN
Two new compounds, (+)-rumphiin (3) and polyalthurea (7), together with seven known ones, 3,4,5-trimethoxy benzoic acid (1), (-)-seselinone (2), cannabisin D (4), allantoin (5), oxostephanine (6) and a mixture of beta-sitosterol (8) and stigmasterol (9) were isolated from the stems of Polyalthia rumphii. The chemical structures of 3 and 7 were elucidated by the combination of spectroscopic data, and the absolute configuration of 3 at C-2 was determined by the matrix method to be R. All compounds were evaluated for their cytotoxicity on four human cancer cell lines, which demonstrated that 3 was a moderate bioactive lignan, and 6 showed significant anticancer activity against SPC-A-1 and BEL-7402 cell lines with IC50 values of 1.47 and 1.73 microg/mL, respectively.
Asunto(s)
Antineoplásicos Fitogénicos/aislamiento & purificación , Ácido Gálico/análogos & derivados , Polyalthia/química , Alantoína/aislamiento & purificación , Ensayos de Selección de Medicamentos Antitumorales , Ácido Gálico/química , Ácido Gálico/aislamiento & purificación , Humanos , Células K562 , Lignanos/aislamiento & purificación , Tallos de la Planta/química , Sitoesteroles/aislamiento & purificación , Estigmasterol/aislamiento & purificaciónRESUMEN
(E)-methyl-4-hydroxy-3,5-dimethoxycinnamate, (E)-methyl-4-hydroxy-3-methoxycinnamate, (E)-methyl-4-hydroxycinnamate, (Z)-methyl-4-hydroxycinnamate, calycanthine, ursolic acid, 2-hydroxy-3-methylanthraquinone, 4-methoxybenzoic acid, 3,4,5-trimethoxybenzoic acid, methyl 4-hydroxybenzoate and allantoin were isolated from the leaves of Palicourea coriacea (Cham.) K. Schum. The structures were deduced by spectral data analysis and comparison with literature values.
Asunto(s)
Extractos Vegetales/química , Rubiaceae/química , Alantoína/aislamiento & purificación , Antraquinonas/aislamiento & purificación , Benzoatos/química , Benzoatos/aislamiento & purificación , Cinamatos/química , Cinamatos/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Naftiridinas/aislamiento & purificación , Hojas de la Planta/química , Triterpenos/aislamiento & purificación , Ácido UrsólicoRESUMEN
OBJECTIVE: To study the water-soluble chemical constituents in root of Achyranthes bidentata. METHOD: The chemical constituents were isolated by silica gel column chromatography and the structures were elucidated by the NMR spectra and physico-chemical properties. RESULT: Seven compounds were obtained and identified as n-butyl-beta-D-fructopyranoside (I), oleanoic acid (II), 3-O-[beta-D-glucopyranosyl], oleanoic acid 28-O-beta-D-glucopyranoside (III), allantoin (IV), 20-hydroxy ecdysone (V), glutamic acid (VI), 3-O-[beta-D-glucopyranosyl], oleanoic acid 28-O-beta-D-glucopyranosyl ester (VII). CONCLUSION: Compounds III-VII were obtained from this plant for the first time.
Asunto(s)
Achyranthes/química , Alantoína/aislamiento & purificación , Ecdisterona/aislamiento & purificación , Ácido Glutámico/aislamiento & purificación , Plantas Medicinales/química , Alantoína/química , Ecdisterona/química , Ácido Glutámico/química , Raíces de Plantas/químicaRESUMEN
OBJECTIVE: To investigate the chemical constituents from the root of Mirabilis jalapa. METHOD: Compounds were isolated from 75% ethanolic extract of the titled herb by silica gel column chromatography, and their structures were elucidated by physical and chemical evidences and spectroscopic analysis. RESULT: Four compounds were obtained and identified as (2, 5-dioxoimidazolidin-4-yl)-urea (1), glycerin monoeicosate (2), boeravinone (3) and beta-sitosterol (4). CONCLUSION: Compound (2) is a new compound, and compound (1) was obtained from this plant for the first time.
Asunto(s)
Alantoína/aislamiento & purificación , Eicosanoides/aislamiento & purificación , Glicerol/análogos & derivados , Glicerol/aislamiento & purificación , Mirabilis/química , Plantas Medicinales/química , Alantoína/química , Eicosanoides/química , Glicerol/química , Raíces de Plantas/química , Sitoesteroles/química , Sitoesteroles/aislamiento & purificaciónRESUMEN
OBJECTIVE: To study the chemical constituents from Lamium maculatum L. var Kansuense. METHOD: The chemical constituents were isolated and repeatedly purified by silica gel column chromatography and the structures were elucidated by the NMR spectra and physico-chemical properties. RESULT: Ten compounds were obtained and they were identified as D-mannitol, beta-sitosterol, stigmasterol, rutin, 3'-methylquercetin-3-O-rutinoside, n-butyl-beta-D-fructopyranoside, daucosterol, acteoside, 20-hydroxyecdysone, allantoin. CONCLUSION: All the compounds were obtained from L. maculatum L. var Kansuense for the first time.
Asunto(s)
Lamiaceae/química , Manitol/aislamiento & purificación , Plantas Medicinales/química , Sitoesteroles/aislamiento & purificación , Alantoína/química , Alantoína/aislamiento & purificación , Ecdisterona/química , Ecdisterona/aislamiento & purificación , Glucósidos/química , Glucósidos/aislamiento & purificación , Manitol/química , Fenoles/química , Fenoles/aislamiento & purificación , Rutina/química , Rutina/aislamiento & purificación , Sitoesteroles/química , Estigmasterol/química , Estigmasterol/aislamiento & purificaciónRESUMEN
Five compounds were isolated from the n-hexane extract and n-butanol of extract Eupolyphaga sinensis. These compounds were identified as octacosanol, beta-sitosterol, batyl alcohol, 2,4-pyrimidinedione and allantoin.