Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Más filtros

Medicinas Complementárias
Métodos Terapéuticos y Terapias MTCI
Bases de datos
Tipo del documento
Intervalo de año de publicación
1.
Ann Vasc Surg ; 29(2): 328-40, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-25449986

RESUMEN

BACKGROUND: The blotchy mouse caused by mutations of ATP7A develops low blood copper and aortic aneurysm and rupture. Although the aortic pathologies are believed primarily due to congenital copper deficiencies in connective tissue, perinatal copper supplementation does not produce significant therapeutic effects, hinting additional mechanisms in the symptom development, such as an independent effect of the ATP7A mutations during adulthood. METHODS: We investigated if bone marrow from blotchy mice contributes to these symptoms. For these experiments, bone marrow from blotchy mice (blotchy marrow group) and healthy littermate controls (control marrow group) was used to reconstitute recipient mice (irradiated male low-density lipoprotein receptor -/- mice), which were then infused with angiotensin II (1,000 ng/kg/min) for 4 weeks. RESULTS: By using Mann-Whitney U test, our results showed that there was no significant difference in the copper concentrations in plasma and hematopoietic cells between these 2 groups. And plasma level of triglycerides was significantly reduced in blotchy marrow group compared with that in control marrow group (P < 0.05), whereas there were no significant differences in cholesterol and phospholipids between these 2 groups. Furthermore, a bead-based multiplex immunoassay showed that macrophage inflammatory protein (MIP)-1ß, monocyte chemotactic protein (MCP)-1, MCP-3, MCP-5, tissue inhibitor of metalloproteinases (TIMP)-1, and vascular endothelial growth factor (VEGF)-A production was significantly reduced in the plasma of blotchy marrow group compared with that in control marrow group (P < 0.05). More important, although angiotensin II infusion increased maximal external aortic diameters in thoracic and abdominal segments, there was no significant difference in the aortic diameters between these 2 groups. Furthermore, aortic ruptures, including transmural breaks of the elastic laminae in the abdominal segment and lethal rupture in the thoracic segment, were observed in blotchy marrow group but not in control marrow group; however, there was no significant difference in the incidence of aortic ruptures between these 2 groups (P = 0.10; Fisher's exact test). CONCLUSIONS: Overall, our study indicated that the effect of bone marrow from blotchy mice during adulthood is dispensable in the regulation of blood copper, plasma cholesterol and phospholipids levels, and aortic pathologies, but contributes to a reduction of MIP-1ß, MCP-1, MCP-3, MCP-5, TIMP-1, and VEGF-A production and triglycerides concentration in plasma. Our study also hints that bone marrow transplantation cannot serve as an independent treatment option.


Asunto(s)
Aneurisma de la Aorta/fisiopatología , Médula Ósea/metabolismo , Cobre/metabolismo , Adenosina Trifosfatasas/genética , Angiotensina II/administración & dosificación , Animales , Aneurisma de la Aorta/sangre , Aneurisma de la Aorta/metabolismo , Rotura de la Aorta/sangre , Rotura de la Aorta/metabolismo , Rotura de la Aorta/fisiopatología , Biomarcadores/sangre , Médula Ósea/fisiopatología , Trasplante de Médula Ósea , Fármacos Cardiovasculares/administración & dosificación , Proteínas de Transporte de Catión/genética , Cobre/sangre , ATPasas Transportadoras de Cobre , Citocinas/sangre , Modelos Animales de Enfermedad , Enzimas/sangre , Femenino , Lípidos/sangre , Masculino , Ratones , Ratones Endogámicos , Receptores de LDL/genética
2.
Heart ; 100(19): 1498-505, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-25060754

RESUMEN

Aneurysms are common in the abdominal and thoracic regions of the aorta. While generally asymptomatic, progression of aneurysms is associated with the devastating consequences of aortic rupture. Current therapeutic options to prevent rupture are restricted to surgical repair, as there remains a lack of validated pharmaceutical approaches. Absence of proven medical therapies may be a consequence of the paucity of knowledge on mechanisms of aneurysmal initiation, progression and rupture. Many potential therapeutic targets have been identified in several widely used animal models of these diseases. A small number of these targets are currently under clinical evaluation, while many more are in preclinical stages of evaluation. The purpose of this review is to: (1) overview current understanding of mechanisms of aneurysmal initiation and progression and (2) summarise medical therapies that have been investigated clinically, as well as highlight future therapeutic targets.


Asunto(s)
Aneurisma de la Aorta , Rotura de la Aorta/prevención & control , Fármacos Cardiovasculares/farmacología , Aneurisma de la Aorta/complicaciones , Aneurisma de la Aorta/metabolismo , Aneurisma de la Aorta/fisiopatología , Rotura de la Aorta/etiología , Enfermedades Asintomáticas/terapia , Progresión de la Enfermedad , Evaluación Preclínica de Medicamentos/métodos , Evaluación Preclínica de Medicamentos/tendencias , Quimioterapia/tendencias , Humanos , Metabolismo , Investigación Biomédica Traslacional/métodos
3.
Arterioscler Thromb Vasc Biol ; 33(9): 2154-61, 2013 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-23825363

RESUMEN

OBJECTIVE: On the basis of previous evidence that polymerase delta interacting protein 2 (Poldip2) increases reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (Nox4) activity in vascular smooth muscle cells, we hypothesized that in vivo knockdown of Poldip2 would inhibit reactive oxygen species production and alter vascular function. APPROACH AND RESULTS: Because homozygous Poldip2 deletion is lethal, Poldip2(+/-) mice were used. Poldip2 mRNA and protein levels were reduced by ≈50% in Poldip2(+/-) aorta, with no change in p22phox, Nox1, Nox2, and Nox4 mRNAs. NADPH oxidase activity was also inhibited in Poldip2(+/-) tissue. Isolated aortas from Poldip2(+/-) mice demonstrated impaired phenylephrine and potassium chloride-induced contractions, increased stiffness, and reduced compliance associated with disruption of elastic lamellae and excessive extracellular matrix deposition. Collagen I secretion was elevated in cultured vascular smooth muscle cells from Poldip2(+/-) mice and restored by H2O2 supplementation, suggesting that this novel function of Poldip2 is mediated by reactive oxygen species. Furthermore, Poldip2(+/-) mice were protected against aortic dilatation in a model of experimental aneurysm, an effect consistent with increased collagen secretion. CONCLUSIONS: Poldip2 knockdown reduces H2O2 production in vivo, leading to increases in extracellular matrix, greater vascular stiffness, and impaired agonist-mediated contraction. Thus, unaltered expression of Poldip2 is necessary for vascular integrity and function.


Asunto(s)
Aorta/metabolismo , Aneurisma de la Aorta/prevención & control , Proteínas Mitocondriales/metabolismo , Proteínas Nucleares/metabolismo , Animales , Aorta/efectos de los fármacos , Aorta/patología , Aorta/fisiopatología , Aneurisma de la Aorta/genética , Aneurisma de la Aorta/metabolismo , Aneurisma de la Aorta/patología , Aneurisma de la Aorta/fisiopatología , Presión Sanguínea , Células Cultivadas , Colágeno Tipo I/metabolismo , Grupo Citocromo b/metabolismo , Dilatación Patológica , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Tejido Elástico/metabolismo , Matriz Extracelular/metabolismo , Regulación de la Expresión Génica , Genotipo , Masculino , Glicoproteínas de Membrana/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Proteínas Mitocondriales/deficiencia , Proteínas Mitocondriales/genética , Miocitos del Músculo Liso/metabolismo , NADH NADPH Oxidorreductasas/metabolismo , NADPH Oxidasa 1 , NADPH Oxidasa 2 , NADPH Oxidasa 4 , NADPH Oxidasas/metabolismo , Proteínas Nucleares/deficiencia , Proteínas Nucleares/genética , Oxidantes/farmacología , Fenotipo , ARN Mensajero/metabolismo , Rigidez Vascular , Vasoconstrictores/farmacología , Vasodilatación
4.
Radiology ; 254(3): 973-81, 2010 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-20177108

RESUMEN

PURPOSE: To prospectively determine if superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance (MR) imaging could help visualize leukocyte phagocytic activities in human abdominal aortic aneurysms (AAAs). MATERIALS AND METHODS: This study was approved by the institutional ethics committee; all patients gave informed consent. Preoperative MR imaging data, including unenhanced and SPIO-enhanced T1-, T2*-, and T2-weighted transverse images of the entire AAA, obtained 1 hour after contrast enhancement from 15 patients (mean age, 72.7 years +/- 8.2; range, 60-83 years), 10 men (mean age, 73.5 years +/- 7.9; range, 60-83 years) and five women (mean age, 71.2 years +/- 9.4; range 60-82), were retrospectively evaluated. Morphologic appearance and semiquantitative and contrast-to-noise ratio (CNR) analyses of the thrombi were performed. Thrombi were analyzed semiquantitatively at microscopy after staining with hematoxylin-eosin, CD68, and CD66b. Levels of promatrix metalloproteinase (pro-MMP)-2 and pro-MMP-9, MMP-2 and MMP-9, and their mRNA located in the thrombus were assessed by using zymography and quantitative reverse transcriptase polymerase chain reaction analysis. Nonparametric statistics of the Spearman rank correlation were calculated to evaluate correlations between the aneurysm thrombus signal level decrease after SPIO and the levels of CD68(+), CD66b(+) cells, pro-MMP-2 and pro-MMP-9, MMP-2 and MMP-9, and MMP-9 mRNA. RESULTS: The pre-SPIO CNRs in the luminal sublayer of the thrombus and the deeper thrombus were -10.20 +/- 12.69 and -5.68 +/-10.38, respectively. After SPIO, the CNRs decreased to -21.34 +/-13.07 (P < .001) and -12.44 +/- 14.56, respectively (P < .012). There was a significant linear correlation between the thrombus signal level decrease and the levels of CD68(+) and CD66b(+) cells, pro-MMP-9, and MMP-9 mRNA (P < .05). CONCLUSION: MR imaging allows in vivo demonstration of SPIO uptake at the luminal interface of the thrombus. This uptake is correlated to the abundance of leukocytes. SUPPLEMENTAL MATERIAL: http://radiology.rsna.org/lookup/suppl/doi:10.1148/radiol.09090657/-/DC1.


Asunto(s)
Aneurisma de la Aorta/metabolismo , Imagen por Resonancia Magnética/métodos , Fagocitosis/fisiología , Trombosis/metabolismo , Anciano , Anciano de 80 o más Años , Análisis de Varianza , Aneurisma de la Aorta/fisiopatología , Medios de Contraste/farmacocinética , Dextranos , Precursores Enzimáticos/metabolismo , Femenino , Óxido Ferrosoférrico/farmacocinética , Gelatinasas/metabolismo , Humanos , Aumento de la Imagen/métodos , Inmunohistoquímica , Nanopartículas de Magnetita , Masculino , Metaloproteinasa 2 de la Matriz/metabolismo , Metaloproteinasa 9 de la Matriz/metabolismo , Persona de Mediana Edad , Estudios Prospectivos , Estudios Retrospectivos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Serina Endopeptidasas/metabolismo , Estadísticas no Paramétricas , Trombosis/fisiopatología
6.
Kardiologiia ; 29(3): 41-5, 1989 Mar.
Artículo en Ruso | MEDLINE | ID: mdl-2733334

RESUMEN

A total of 325 patients with occlusions and aneurysms of the aorta and its main branches were investigated preoperatively. The patients were divided into 2 groups; in one of those (32 patients), esophageal electrostimulation of the heart was used for the diagnosis of latent coronary insufficiency. In addition to the degree of myocardial ischemia, the efficiency of antianginal drugs and the results of preoperative medication were assessed here. Comparative analysis of the results demonstrated a higher sensitivity and diagnostic value of esophageal electrostimulation, as compared to routine lead tests, which, in combination with the test's simplicity and noninvasiveness as well as possible identification of diagnostically-meaningful criteria, makes it a method of choice.


Asunto(s)
Aneurisma de la Aorta/fisiopatología , Arteriopatías Oclusivas/fisiopatología , Estimulación Cardíaca Artificial , Vasos Coronarios/fisiopatología , Pierna/irrigación sanguínea , Adulto , Anciano , Aneurisma de la Aorta/cirugía , Arteriopatías Oclusivas/cirugía , Esófago , Femenino , Insuficiencia Cardíaca/prevención & control , Humanos , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias/prevención & control , Cuidados Preoperatorios
7.
Anaesthesia ; 42(5): 491-7, 1987 May.
Artículo en Inglés | MEDLINE | ID: mdl-3592175

RESUMEN

The purpose of this study was to compare the peri-operative conditions produced by intrathecal morphine with those that result from conventional analgesia in aortic aneurysm surgery. Low-dose intrathecal morphine provides a level of analgesia during operation indistinguishable from that of moderate doses of parenteral opiate, but is no more effective in attenuating autonomic responses to the procedure. Analgesia is prolonged into the postoperative period, does not cause clinically evident respiratory depression and can be enhanced by small doses of intravenous opiate. The principal advantage of intrathecal morphine is the avoidance of irregular and inadequate pain relief in the early, and most painful, part of the postoperative period. Low-dose intrathecal morphine appears safe, effective and feasible in aortic aneurysm surgery and provides an alternative to traditional management with parenteral opiates.


Asunto(s)
Aneurisma de la Aorta/cirugía , Morfina/administración & dosificación , Adulto , Anciano , Aneurisma de la Aorta/fisiopatología , Hemodinámica , Humanos , Inyecciones Espinales , Periodo Intraoperatorio , Masculino , Persona de Mediana Edad , Morfina/uso terapéutico , Opio/uso terapéutico , Dolor Postoperatorio/tratamiento farmacológico
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA