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1.
Med Sci Monit ; 26: e924658, 2020 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-32738135

RESUMEN

BACKGROUND Anxiety is one of the common comorbidities of Tourette syndrome (TS). The serotonin (5-HT) system is involved in both TS and anxiety. Jian-pi-zhi-dong decoction (JPZDD) is widely used. However, the mechanism remains unknown. In this study, a rat model of TS and comorbid anxiety was used to evaluate the effect of JPZDD on 5-HT and its receptor. MATERIAL AND METHODS 48 rats were divided into 4 groups randomly (n=12). The model was established by empty water bottle stimulation plus iminodipropionitrile injection for 3 weeks. Then the control and model groups were gavaged with saline, while the treatment groups were gavaged with fluoxetine hydrochloride (Flx) or JPZDD. Body weights were measured, and behavioral tests were evaluated with stereotypy and elevated plus maze. The morphologic characters were observed by hematoxylin and eosin staining. The content of 5-HT was detected by enzyme-linked immunosorbent assay and high-performance liquid chromatography. The expression of 5-HT2C receptor was detected by western blot and quantitative polymerase chain reaction. RESULTS The stereotypy score was lower and the time spent in the open arm was longer in the JPZDD group compared with the model group. After the treatment of Flx or JPZDD, the structure of neurons became gradually normal and the cells were arranged neatly. The contents of 5-HT in the treatment groups were higher compared with the model group in the striatum. The expression of 5-HT2C mRNA in the striatum of JPZDD and Flx groups decreased compared with the model group, and the JPZDD group was lower than the Flx group. CONCLUSIONS JPZDD alleviated both tic and anxiety symptoms and the mechanism may be via reducing the expression of 5-HT2C mRNA in the striatum, increasing the concentration of 5-HT, and enhancing the activity of the 5-HT system, which in turn exerts neuro-inhibition.


Asunto(s)
Antidepresivos de Segunda Generación/farmacología , Ansiedad/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Receptor de Serotonina 5-HT2C/genética , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Síndrome de Tourette/tratamiento farmacológico , Animales , Ansiedad/inducido químicamente , Ansiedad/genética , Ansiedad/fisiopatología , Conducta Animal/efectos de los fármacos , Corteza Cerebral/efectos de los fármacos , Corteza Cerebral/metabolismo , Corteza Cerebral/fisiopatología , Cuerpo Estriado/efectos de los fármacos , Cuerpo Estriado/metabolismo , Cuerpo Estriado/fisiopatología , Modelos Animales de Enfermedad , Fluoxetina/farmacología , Expresión Génica , Humanos , Masculino , Aprendizaje por Laberinto/efectos de los fármacos , Nitrilos/administración & dosificación , Ratas , Ratas Sprague-Dawley , Receptor de Serotonina 5-HT2C/metabolismo , Serotonina/metabolismo , Síndrome de Tourette/inducido químicamente , Síndrome de Tourette/genética , Síndrome de Tourette/fisiopatología , Resultado del Tratamiento
2.
Physiol Behav ; 209: 112534, 2019 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-31071338

RESUMEN

To improve our understanding of the effects of standardized extract of Ginkgo biloba (EGb) as a cognitive enhancer, we investigated the conditioned lick suppression-induced expression (mRNA and protein) of the GluN2B-containing N-methyl-D-aspartic acid receptor (GluN2B-NMDAR), serotonin (5-HT) 1A receptor (5-HT1AR), gamma-aminobutyric acid type A receptor (GABAAR) and glial fibrillary acidic protein (GFAP) in the dorsal hippocampal formation (dHF) of untreated and EGb-treated (0.25, 0.5 and 1.0 g.kg-1) groups of rats. To substantiate our data, we analysed the molecular changes in dHF following treatment with vehicle, with agonists or antagonists of GABAAR, GluN2B-NMDAR and 5-HT1AR or with one of these antagonists prior to EGb and fear memory acquisition. Additionally, we performed a pharmacological analysis of the drug-receptor-receptor interactions and their supplemental role in fear memory by blocking individual receptors and analysed the possible changes in expression level with each of the other receptors in the study as well as astrocytes. Our data show for the first time that EGb treatment not only upregulated GluN2B, GABAAR-α5, and GFAP compared with the control but also differentially upregulated GABAAR-α1 in the dHF and 5HT1AR in the CA3. We found that the activation of GABAARs (diazepam) and the inactivation of GluN2B-NMDARs (Ro25-6981) or 5-HT1AR ((S)-WAY100135) resulted in memory impairment. Further, higher doses of EGb treatment reversed the effect of blocking GluN2B (P < 0.001) and 5-HT1AR (P < 0.001). Here, treatment with Ro25-6981 + EGb or (S)-WAY100135 + EGb prevented the impairment of the acquisition of lick suppression in association with the upregulation or prevention of the downregulation of Grin2b expression as well as the expression of GluN2B-NMDA and/or α1 and α5 subunit-containing GABAAR in the CA1 (P < 0.0001). Our data are in line with previous findings concerning the necessity of GluN2B for fear memory formation and add to the current knowledge of the role of the GABAAR-α1 and -α5 subunits and of GluN2B as a target of cognitive enhancers. Furthermore, our data show that these receptors play a complementary role in controlling the neural circuitry in the dHF that seems to be essential to conditioned lick suppression and the modulatory effects of EGb.


Asunto(s)
Condicionamiento Operante/efectos de los fármacos , Hipocampo/efectos de los fármacos , Red Nerviosa/efectos de los fármacos , Extractos Vegetales/farmacología , Receptor de Serotonina 5-HT1A/efectos de los fármacos , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidores , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Animales , Región CA3 Hipocampal/efectos de los fármacos , Región CA3 Hipocampal/metabolismo , Diazepam/farmacología , Moduladores del GABA/farmacología , Ginkgo biloba , Masculino , Memoria/efectos de los fármacos , Fenoles/farmacología , Piperazinas/farmacología , Piperidinas/farmacología , Ratas , Ratas Wistar
3.
J Pain ; 20(1): 16.e1-16.e16, 2019 01.
Artículo en Inglés | MEDLINE | ID: mdl-30102991

RESUMEN

Acupoint catgut embedding (ACE) is a widely used traditional Chinese medicine method to manage various diseases, including chronic inflammatory pain. We sought to assess the possible analgesic effects of ACE in comparison with electroacupuncture (EA) and to study the analgesic mechanisms of ACE in a rat model of inflammatory pain induced by injection of complete Freund's adjuvant (CFA) into the hind paw of rats. The von Frey, radiant heat, and gait analysis tests were performed to evaluate the analgesic effects of ACE and EA, and Western blot and immunohistochemistry assays were carried out to determine the molecular mechanisms of ACE. ACE treatments were administered every 4 days or every week with different acupoints (ipsilateral, contralateral, or bilateral ST36 and GB30 acupoints). The most effective ACE strategy for attenuating the nocifensive response induced by CFA injection was performing ACE once a week at ipsilateral ST36 in combination with GB30. EA treatment every other day at ipsilateral ST36 and GB30 showed comparable analgesic effects. ACE inhibited the increased activation of the GluN1 subunit of the N-methyl-d-aspartate receptor and the subsequent Ca2+-dependent signals (CaMKII, ERK, and CREB) that take place in response to CFA. The effects of ACE were similar to intrathecal injection of vilazodone (a serotonin 1A receptor [5-HT1AR] agonist) and were blocked by WAY-100635 (a 5-HT1AR antagonist). In summary, we show that ACE attenuates CFA-induced inflammatory pain in rats by activating spinal 5-HT1AR and by inhibiting the phosphorylation of GluN1, thus, inhibiting the activation of Ca2+-dependent signaling cascades. PERSPECTIVE: This article presents the novel evidence concerning the spinal 5-HT1AR activation-related molecular signaling of ACE analgesia in a rat model of CFA-induced inflammatory pain. This work may help clinicians to verify the effectiveness of ACE analgesia and to better understand the underlying mechanism.


Asunto(s)
Analgesia por Acupuntura , Puntos de Acupuntura , Catgut , Electroacupuntura , Inflamación/metabolismo , Manejo del Dolor , Dolor/metabolismo , Receptor de Serotonina 5-HT1A/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Agonistas del Receptor de Serotonina 5-HT1/farmacología , Analgesia por Acupuntura/métodos , Animales , Modelos Animales de Enfermedad , Electroacupuntura/métodos , Adyuvante de Freund/farmacocinética , Inflamación/inducido químicamente , Masculino , Dolor/inducido químicamente , Fosforilación , Ratas , Ratas Sprague-Dawley , Agonistas del Receptor de Serotonina 5-HT1/administración & dosificación , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Médula Espinal/efectos de los fármacos , Clorhidrato de Vilazodona/farmacología
4.
Can J Physiol Pharmacol ; 97(2): 90-98, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30462556

RESUMEN

Although depression and cardiovascular diseases are related, the role of antidepressants such as fluoxetine (increasing serotonin levels) within cardiac regulation remains unclear. We aimed to determine whether fluoxetine modifies the pharmacological profile of serotonergic influence on vagal cardiac outflow. Rats were treated with fluoxetine (10 mg/kg per day; p.o.) for 14 days or equivalent volumes of drinking water (control group); then, they were pithed and prepared for vagal stimulation. Bradycardic responses were obtained by electrical stimulation of the vagal fibers (3, 6, and 9 Hz) or i.v. acetylcholine (ACh; 1, 5, and 10 µg/kg). The i.v. administration of 5-hydroxytryptamine (5-HT; 10 and 50 µg/kg) inhibited the vagally induced bradycardia. 5-CT (5-HT1/7 agonist) and L-694,247 (5-HT1D agonist) mimicked the serotonin inhibitory effect while α-methyl-5-HT (5-HT2 agonist) was devoid of any action. SB269970 (5-HT7 antagonist) did not abolish 5-CT inhibitory action on the electrically induced bradycardia. Pretreatment with LY310762 (5-HT1D antagonist) blocked the effects induced by L-694,247 and 5-CT. 5-HT and 5-CT failed to modify the bradycardia induced by exogenous ACh. Our outcomes suggest that fluoxetine treatment modifies 5-HT modulation on heart parasympathetic neurotransmission in rats, evoking inhibition of the bradycardia via prejunctional 5-HT1D in pithed rats.


Asunto(s)
Antidepresivos de Segunda Generación/farmacología , Bradicardia/tratamiento farmacológico , Fluoxetina/farmacología , Receptor de Serotonina 5-HT1D/metabolismo , Nervio Vago/efectos de los fármacos , Administración Oral , Animales , Antidepresivos de Segunda Generación/uso terapéutico , Bradicardia/etiología , Depresión/complicaciones , Depresión/tratamiento farmacológico , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Fluoxetina/uso terapéutico , Corazón/inervación , Frecuencia Cardíaca/efectos de los fármacos , Humanos , Oxadiazoles/farmacología , Fenoles/farmacología , Ratas , Ratas Wistar , Serotonina/farmacología , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Agonistas de Receptores de Serotonina/metabolismo , Sulfonamidas/farmacología , Triptaminas/farmacología , Nervio Vago/metabolismo
5.
J Ethnopharmacol ; 231: 453-463, 2019 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-30545804

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: Piper auritum Kunth is employed as an aphrodisiac in the traditional medicine, but corroborative evidence for such effect is scarce. AIM OF THE STUDY: The pro-sexual effect of an aqueous extract of P. auritum and its possible mechanisms were analyzed in two paradigms of male sexual function. MATERIAL AND METHODS: Effects of an aqueous extract of P. auritum (PA, single administration) were investigated in the fictive ejaculation, and copulatory behavior paradigms in sexually sluggish male rats. WAY 100635 (antagonist of 5-HT1A receptors), atosiban (antagonist of oxytocinergic receptors), L-NAME (inhibitor of the nitric oxide synthase) and baclofen (antagonist of GABAB receptors) were used as pre-treatments in order to investigate the role of different neurotransmitter systems in PA actions. Chemical profile of PA was determined by Gases Chromatography and Ultra Performance Chromatography-Electrospray Ionization-Masses Spectrometry (UPLC-ESI-MS). RESULTS: In males with retarded ejaculation, PA stimulated ejaculatory behavior and recovered electromyographic activity of pelvic musculature participating in seminal emission and ejaculation. All pre-treatments blocked stimulating effects of PA on the fictive ejaculation; additionally WAY 100635 interfered with PA actions on ejaculatory behavior. Safrol, apigenin dimethylether, myristicin, vaccihein A, sakuranin and sakuranetin flavonoids, were main constituents of PA, with possible participation in its pro-sexual effects. CONCLUSIONS: Pro-sexual effects of P. auritum elicited at level of ejaculation were mediated by several neurotransmitter systems, among which serotonin and its 5-HT1A receptors play an important role. Present findings support P. auritum reputation as an aphrodisiac, with potential use in delayed ejaculation disorder.


Asunto(s)
Afrodisíacos/farmacología , Piper , Extractos Vegetales/farmacología , Conducta Sexual/efectos de los fármacos , Animales , Afrodisíacos/química , Conducta Animal/efectos de los fármacos , Eyaculación/efectos de los fármacos , Femenino , Masculino , Fitoquímicos/análisis , Fitoquímicos/farmacología , Piper/química , Piperazinas/farmacología , Extractos Vegetales/química , Hojas de la Planta/química , Piridinas/farmacología , Ratas Wistar , Receptor de Serotonina 5-HT1A/fisiología , Antagonistas del Receptor de Serotonina 5-HT1/farmacología
6.
Physiol Behav ; 196: 119-125, 2018 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-30179595

RESUMEN

Low frequency stimulation (LFS) has been proposed as a method in the treatment of epilepsy, but its anticonvulsant mechanism is still unknown. In the current study, the hippocampal CA1 region was microinjected with NAD-299 (a selective 5-HT1A antagonist), and its role in mediating the inhibitory action of LFS on amygdala kindling was investigated. Male Wistar rats were kindled by amygdala stimulation in a semi-rapid kindling manner (12 stimulations per day). LFS (0.1 ms pulse duration at 1 Hz, 200 pulses, 50-150 µA) was applied at 5 min after termination of daily kindling stimulations. NAD (a selective 5-HT1A antagonist) was microinjected into the CA1 region of the hippocampus at the doses of 2.5 and 5 µg/1 µl. An open field test was also run to determine the motor activity of animals in different experimental groups. The application of LFS following daily kindling stimulations reduced the behavioral seizure stages, afterdischarge duration, and stage 5 seizure duration and increased the latency to stage 4 seizure compared to the kindled group. However, microinjection of NAD at the doses of 5 µg/1 µl, but not 2.5 µg/1 µl, blocked the inhibitory effect of LFS on behavioral and electrophysiological parameters in kindled animals. It could be presumed that 5-HT1A receptors in the CA1 area are involved in mediating the antiepileptic effects of LFS.


Asunto(s)
Región CA1 Hipocampal/metabolismo , Terapia por Estimulación Eléctrica , Receptor de Serotonina 5-HT1A/metabolismo , Convulsiones/metabolismo , Convulsiones/terapia , Amígdala del Cerebelo/efectos de los fármacos , Amígdala del Cerebelo/metabolismo , Animales , Benzopiranos/farmacología , Región CA1 Hipocampal/efectos de los fármacos , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Neuroestimuladores Implantables , Excitación Neurológica , Masculino , Actividad Motora/efectos de los fármacos , Actividad Motora/fisiología , Ratas Wistar , Antagonistas del Receptor de Serotonina 5-HT1/farmacología
7.
Behav Pharmacol ; 29(5): 437-444, 2018 08.
Artículo en Inglés | MEDLINE | ID: mdl-29521667

RESUMEN

The present study investigated the effects of estradiol (E2) on ingestive behavior after activation of 5-HT1A receptors in the lateral hypothalamus (LH) of female rats habituated to eat a wet mash diet. Ovariectomized rats treated with corn oil (OVX) or estradiol cypionate (OVX+E) received local injections into the LH of vehicle or an agonist of 5-HT1A receptors, 8-hydroxy-2-(di-n-propylamino)-tetralin (8-OH-DPAT; at a dose of 6 nmol). To determine the involvement of these receptors in food intake, some animals were pretreated with N-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-N-(2-pyridinyl) cyclohexane carboxamide maleate (WAY-100635, a 5-HT1A receptor full antagonist, at a dose of 0.37 nmol), followed by the injection of the agonist 8-OH-DPAT or its vehicle. The results showed that the injection of 8-OH-DPAT into the LH of OVX rats significantly increased food intake, and the duration and frequency of this behavior. The pretreatment with E2 suppressed the hyperphagic response induced by 8-OH-DPAT in OVX animals. The inhibition of 5-HT1A receptors after pretreatment with WAY-100635 blocked the hyperphagic effects evoked by 8-OH-DPAT in OVX. These results indicate that the activity of LH 5-HT1A receptors could be affected by blood E2 levels.


Asunto(s)
Estradiol/farmacología , Conducta Alimentaria/efectos de los fármacos , Receptor de Serotonina 5-HT1A/fisiología , 8-Hidroxi-2-(di-n-propilamino)tetralin/farmacología , Animales , Ingestión de Alimentos/efectos de los fármacos , Estradiol/análogos & derivados , Estradiol/metabolismo , Femenino , Área Hipotalámica Lateral/efectos de los fármacos , Área Hipotalámica Lateral/metabolismo , Hipotálamo/efectos de los fármacos , Ovariectomía , Piperazinas , Piridinas , Ratas , Ratas Wistar , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Antagonistas de la Serotonina/farmacología , Agonistas de Receptores de Serotonina/farmacología
8.
Mol Neurobiol ; 55(10): 7802-7821, 2018 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-29468562

RESUMEN

We investigated whether a specific serotonin (5-HT) receptor-mediated mechanism was involved in dextromethorphan (DM)-induced serotonergic behaviors. We firstly observed that the activation of 5-HT1A receptor, but not 5-HT2A receptor, contributed to DM-induced serotonergic behaviors in mice. We aimed to determine whether the upregulation of 5-HT1A receptor induced by DM facilitates the specific induction of certain PKC isoform, because previous reports suggested that 5-HT1A receptor activates protein kinase C (PKC). A high dose of DM (80 mg/kg, i.p.) induced a selective induction of PKCδ out of PKCα, PKCßI, PKCßII, PKCξ, and PKCδ in the hypothalamus of wild-type (WT) mice. More importantly, 5-HT1A receptor co-immunoprecipitated PKCδ in the presence of DM. Consistently, rottlerin, a pharmacological inhibitor of PKCδ, or PKCδ knockout significantly protected against increases in 5-HT1A receptor gene expression, 5-HT turnover rate, and serotonergic behaviors induced by DM. Treatment with DM resulted in an initial increase in nuclear factor erythroid-2-related factor 2 (Nrf2) nuclear translocation and DNA-binding activity, γ-glutamylcysteine (GCL) mRNA expression, and glutathione (GSH) level. This compensative induction was further potentiated by rottlerin or PKCδ knockout. However, GCL mRNA and GSH/GSSG levels were decreased 6 and 12 h post-DM. These decreases were attenuated by PKCδ inhibition. Our results suggest that interaction between 5-HT1A receptor and PKCδ is critical for inducing DM-induced serotonergic behaviors and that inhibition of PKCδ attenuates the serotonergic behaviors via downregulation of 5-HT1A receptor and upregulation of Nrf2-dependent GSH synthesis.


Asunto(s)
Conducta Animal , Regulación hacia Abajo , Glutatión/biosíntesis , Factor 2 Relacionado con NF-E2/metabolismo , Proteína Quinasa C-delta/metabolismo , Receptor de Serotonina 5-HT1A/metabolismo , Serotonina/metabolismo , Regulación hacia Arriba , Acetofenonas/farmacología , Animales , Benzopiranos/farmacología , Núcleo Celular/efectos de los fármacos , Núcleo Celular/metabolismo , Dextrometorfano , Glutamato-Cisteína Ligasa/genética , Glutamato-Cisteína Ligasa/metabolismo , Disulfuro de Glutatión/metabolismo , Hipotálamo/metabolismo , Hipotermia Inducida , Isoenzimas/metabolismo , Ratones Endogámicos C57BL , Ratones Noqueados , Fármacos Neuroprotectores/farmacología , Fosforilación/efectos de los fármacos , Piperazinas/farmacología , Piridinas/farmacología , ARN Mensajero/genética , ARN Mensajero/metabolismo , Receptor de Serotonina 5-HT2A/metabolismo , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Factores de Tiempo
9.
Am J Physiol Renal Physiol ; 308(9): F1032-40, 2015 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-25694482

RESUMEN

After spinal cord injury (SCI), the neurogenic bladder is observed to develop asynchronous bladder and external urethral sphincter (EUS) contractions in a condition known as detrusor-sphincter dyssnergia (DSD). Activation of the EUS spinal controlling center located at the upper lumbar spinal cord may contribute to reduce EUS dyssynergic contractions and decrease urethral resistance during voiding. However, this mechanism has not been well studied. This study aimed at evaluating the effects of epidural stimulation (EpS) over the spinal EUS controlling center (L3) in combination with a serotonergic receptor agonist on EUS relaxation in naive rats and chronic (6-8 wk) T8 SCI rats. Cystometrogram and EUS electromyography (EMG) were obtained before and after the intravenous administration of 5HT-1A receptor agonist and antagonist. The latency, duration, frequency, amplitude, and area under curve of EpS-evoked EUS EMG responses were analyzed. EpS on L3 evoked an inhibition of EUS tonic contraction and an excitation of EUS intermittent bursting/relaxation correlating with urine expulsion in intact rats. Combined with a 5HT-1A receptor agonist, EpS on L3 evoked a similar effect in chronic T8 SCI rats to reduce urethral contraction (resistance). This study examined the effect of facilitating the EUS spinal controlling center to switch between urine storage and voiding phases by using EpS and a serotonergic receptor agonist. This novel approach of applying EpS on the EUS controlling center modulates EUS contraction and relaxation as well as reduces urethral resistance during voiding in chronic SCI rats with DSD.


Asunto(s)
Terapia por Estimulación Eléctrica/métodos , Traumatismos de la Médula Espinal/complicaciones , Médula Espinal/fisiopatología , Uretra/inervación , Vejiga Urinaria Neurogénica/terapia , Urodinámica , Animales , Modelos Animales de Enfermedad , Electromiografía , Femenino , Vértebras Lumbares , Ratas Sprague-Dawley , Reflejo , Agonistas del Receptor de Serotonina 5-HT1/farmacología , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Traumatismos de la Médula Espinal/fisiopatología , Factores de Tiempo , Uretra/efectos de los fármacos , Vejiga Urinaria Neurogénica/etiología , Vejiga Urinaria Neurogénica/fisiopatología , Urodinámica/efectos de los fármacos
10.
Pharmacol Biochem Behav ; 128: 41-9, 2015 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-25449359

RESUMEN

The present study investigated the anxiolytic-like effects of spinosin, one of the major flavonoids in Ziziphi Spinosae Semen (ZSS), in experimental models of anxiety compared with a known anxiolytic, diazepam. Repeated treatment with spinosin (2.5 and 5mg/kg/day, p.o.) significantly increased the percentage of entries into and time spent on the open arms of the elevated plus maze compared with the control group. In the light/dark box test, spinosin exerted an anxiolytic-like effect at 5mg/kg. In the open-field test, 5mg/kg spinosin increased the number of central entries. Spinosin did not affect spontaneous activity. The anxiolytic-like effects of spinosin in the elevated plus maze, light/dark box test, and open field test were blocked by the γ-aminobutyric acid-A (GABAA) receptor antagonist flumazenil (3mg/kg, i.p.) and 5-hydroxytryptamine-1A (5-HT1A) receptor antagonist WAY-100635 (1mg/kg, i.p.). These results suggest that spinosin exerts anxiolytic-like effects, and its mechanism of action appears to be modulated by GABAA and 5-HT1A receptors.


Asunto(s)
Ansiolíticos/farmacología , Flavonoides/farmacología , Serotonina/fisiología , Ácido gamma-Aminobutírico/fisiología , Animales , Ansiedad/tratamiento farmacológico , Ansiedad/fisiopatología , Ansiedad/psicología , Conducta Animal/efectos de los fármacos , Conducta Animal/fisiología , Medicamentos Herbarios Chinos/farmacología , Flumazenil/farmacología , Antagonistas de Receptores de GABA-A/farmacología , Masculino , Aprendizaje por Laberinto/efectos de los fármacos , Aprendizaje por Laberinto/fisiología , Ratones , Ratones Endogámicos ICR , Actividad Motora/efectos de los fármacos , Actividad Motora/fisiología , Piperazinas/farmacología , Piridinas/farmacología , Receptor de Serotonina 5-HT1A/fisiología , Receptores de GABA-A/fisiología , Antagonistas del Receptor de Serotonina 5-HT1/farmacología
11.
Metab Brain Dis ; 30(1): 47-55, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-24807589

RESUMEN

Irritable bowel syndrome (IBS) is induced by dysfunction of central nervous and peripheral intestinal systems, which affects an estimated 10-15% population worldwide annually. Stress-related psychiatric disorders including depression and anxiety are often comorbid with gastrointestinal function disorder, such as IBS. However, the mechanism of IBS still remains unknown. Curcumin is a biologically active phytochemical presents in turmeric and has pharmacological actions that benefit patients with depression and anxiety. Our study found that IBS rats showed depression- and anxiety-like behaviors associated with decreased 5-HT (serotonin), BDNF (Brain-derived neurotrophic factor) and pCREB (phosphorylation of cAMP response element-binding protein) expression in the hippocampus after chronic acute combining stress (CAS). However, these decreased parameters were obviously increased in the colonic after CAS. Curcumin (40 mg/kg) reduced the immobility time of forced swimming and the number of buried marbles in behavioral tests of CAS rats. Curcumin also decreased the number of fecal output and abdominal withdrawal reflex (AWR) scores in response to graded distention. Moreover, curcumin increased serotonin, BDNF and pCREB levels in the hippocampus, but they were decreased in the colonic of CAS rats. 5-HT(1A) receptor antagonist NAN-190 reversed the effects of curcumin on behaviors and the changes of intestine, pCREB and BDNF expression, which are related to IBS. These results suggested that curcumin exerts the effects on IBS through regulating neurotransmitters, BDNF and CREB signaling both in the brain and peripheral intestinal system.


Asunto(s)
Curcumina/uso terapéutico , Sistema Nervioso Entérico/fisiopatología , Hipocampo/fisiopatología , Síndrome del Colon Irritable/tratamiento farmacológico , Serotonina/fisiología , Animales , Ansiedad/tratamiento farmacológico , Ansiedad/fisiopatología , Factor Neurotrófico Derivado del Encéfalo/biosíntesis , Colon/metabolismo , Curcumina/farmacología , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/biosíntesis , Defecación , Diazepam/farmacología , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Motilidad Gastrointestinal/efectos de los fármacos , Hipocampo/metabolismo , Imipramina/farmacología , Síndrome del Colon Irritable/fisiopatología , Masculino , Fosforilación , Esfuerzo Físico , Piperazinas/farmacología , Presión/efectos adversos , Procesamiento Proteico-Postraduccional , Ratas , Ratas Sprague-Dawley , Receptor de Serotonina 5-HT1A/biosíntesis , Receptor de Serotonina 5-HT1A/efectos de los fármacos , Receptor de Serotonina 5-HT1A/fisiología , Serotonina/biosíntesis , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Transducción de Señal , Estrés Fisiológico/fisiología , Estrés Psicológico/fisiopatología , Regulación hacia Arriba/efectos de los fármacos
12.
Sci Rep ; 4: 4433, 2014 Mar 24.
Artículo en Inglés | MEDLINE | ID: mdl-24658501

RESUMEN

Depression is a major psychiatric disorder affecting nearly 21% of the world population and imposes a substantial health burden on society. Current available antidepressants are not adequate to meet the clinical needs. Here we report that auraptenol, an active component of the traditional Chinese medicine, angelicae dahuricae radix, had antidepressant-like effects in mice models of depression. In mouse forced swimming test and tail suspension test, two validated models of depression, auraptenol dose-dependently decreased the immobility duration within the dose range of 0.05-0.4 mg/kg. In addition, the antidepressant-like effects of auraptenol was significantly averted by a selective serotonin 5-HT1A receptor antagonist WAY100635 (1 mg/kg). These doses that affected the immobile response did not affect locomotor activity. In summary, this study for the first time identified an active component from the herbal medicine angelicae dahuricae radix that possesses robust antidepressant-like efficacy in mice. These data support further exploration for the possibility of developing auraptenol as a novel antidepressant agent in the treatment of major depression disorders.


Asunto(s)
Angelica/química , Antidepresivos/farmacología , Cumarinas/farmacología , Depresión/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Fitoterapia , Animales , Antidepresivos/antagonistas & inhibidores , Antidepresivos/aislamiento & purificación , Conducta Animal/efectos de los fármacos , Cumarinas/antagonistas & inhibidores , Cumarinas/aislamiento & purificación , Depresión/fisiopatología , Depresión/psicología , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Medicamentos Herbarios Chinos/aislamiento & purificación , Suspensión Trasera , Masculino , Ratones , Ratones Endogámicos C57BL , Actividad Motora/efectos de los fármacos , Piperazinas/farmacología , Piridinas/farmacología , Receptores de Serotonina 5-HT1/metabolismo , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Natación
13.
Sci Rep ; 3: 3377, 2013 Nov 29.
Artículo en Inglés | MEDLINE | ID: mdl-24287473

RESUMEN

Common chemotherapeutic agents such as vincristine often cause neuropathic pain during cancer treatment in patients. Such neuropathic pain is refractory to common analgesics and represents a challenging clinical issue. Angelicae dahuricae radix is an old traditional Chinese medicine with demonstrated analgesic efficacy in humans. However, the active component(s) that attribute to the analgesic action have not been identified. This work described the anti-hyperalgesic effect of one coumarin component, auraptenol, in a mouse model of chemotherapeutic agent vincristine-induced neuropathic pain. We reported that auraptenol dose-dependently reverted the mechanical hyperalgesia in mice within the dose range of 0.05-0.8 mg/kg. In addition, the anti-hyperalgesic effect of auraptenol was significantly blocked by a selective serotonin 5-HT1A receptor antagonist WAY100635 (1 mg/kg). Within the dose range studied, auraptenol did not significantly alter the general locomotor activity in mice. Taken together, this study for the first time identified an active component from the herbal medicine angelicae dahuricae radix that possesses robust analgesic efficacy in mice. These data support further studies to assess the potential of auraptenol as a novel analgesic for the management of neuropathic pain.


Asunto(s)
Analgésicos/farmacología , Cumarinas/farmacología , Hiperalgesia/inducido químicamente , Hiperalgesia/tratamiento farmacológico , Receptores de Serotonina 5-HT1/metabolismo , Vincristina/efectos adversos , Animales , Modelos Animales de Enfermedad , Medicina de Hierbas/métodos , Hiperalgesia/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Actividad Motora/efectos de los fármacos , Neuralgia/inducido químicamente , Neuralgia/tratamiento farmacológico , Neuralgia/metabolismo , Fitoterapia/métodos , Receptor de Serotonina 5-HT1A , Serotonina/metabolismo , Antagonistas del Receptor de Serotonina 5-HT1/farmacología
14.
Br J Pharmacol ; 170(4): 859-70, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23902597

RESUMEN

BACKGROUND AND PURPOSE: The beat-by-beat fluctuation (dynamics) of heart rate (HR) depends on centrally mediated control of the autonomic nervous system (ANS) reflecting the physiological state of an organism. 5-HT1A receptors are implicated in affective disorders,associated with ANS dysregulation which increases cardiac risk but their role in autonomic HR regulation under physiological conditions is insufficiently characterized. EXPERIMENTAL APPROACH: The effects of subcutaneously administered 5-HT1A receptor ligands on HR dynamics were investigated in C57BL/6 mice during stress-free conditions and emotional challenge (recall of fear conditioned to an auditory stimulus and novelty exposure) using time domain and non-linear HR analyses. KEY RESULTS: Pre-training treatment with of 8-OH-DPAT (0.5 mg·kg(-1) , s.c.) prevented conditioned tachycardia in the retention test indicating impaired fear memory. Pretest 5-HT1A receptor activation by 8-OH-DPAT (0.5 but not 0.1 and 0.02 mg·kg(-1) ) caused bradycardia and increased HR variability. 8-OH-DPAT (0.5 mg·kg(-1) ) lowered the unconditioned and conditioned tachycardia from ∼750 to ∼550 bpm, without changing the conditioned HR response to the sound. 8-OH-DPAT induced profound QT prolongation and bradyarrhythmic episodes. Non-linear analysis indicated a pathological state of HR dynamics after 8-OH-DPAT (0.5 mg·kg(-1) ) with ANS hyperactivation impairing HR adaptability. The 5-HT1A receptor antagonist WAY-100635 (0.03 mg·kg(-1) ) blocked these effects of 8-OH-DPAT. CONCLUSIONS AND IMPLICATIONS: Pre-training 5-HT1A receptor activation by 8-OH-DPAT (0.5 mg·kg(-1) ) impaired memory of conditioned auditory fear based on an attenuated HR increase, whereas pretest administration did not prevent the fear-conditioned HR increase but induced pathological HR dynamics through central ANS dysregulation with cardiac effects similar to acute SSRI overdose.


Asunto(s)
Sistema Nervioso Autónomo/metabolismo , Conducta Animal , Encéfalo/metabolismo , Condicionamiento Psicológico , Miedo , Frecuencia Cardíaca , Corazón/inervación , Receptor de Serotonina 5-HT1A/metabolismo , Estimulación Acústica , Animales , Sistema Nervioso Autónomo/efectos de los fármacos , Conducta Animal/efectos de los fármacos , Encéfalo/efectos de los fármacos , Condicionamiento Psicológico/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Electrocardiografía , Emociones , Miedo/efectos de los fármacos , Frecuencia Cardíaca/efectos de los fármacos , Ligandos , Masculino , Recuerdo Mental/efectos de los fármacos , Ratones , Ratones Endogámicos C57BL , Dinámicas no Lineales , Receptor de Serotonina 5-HT1A/efectos de los fármacos , Agonistas del Receptor de Serotonina 5-HT1/farmacología , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Procesamiento de Señales Asistido por Computador , Factores de Tiempo
15.
Artículo en Inglés | MEDLINE | ID: mdl-23791616

RESUMEN

Cannabidiol (CBD) is a non-psychotomimetic compound from Cannabis sativa plant that produces antipsychotic effects in rodents and humans. It also reverses L-dopa-induced psychotic symptoms and improves motor function in Parkinson's patients. This latter effect raised the possibility that CBD could have beneficial effects on motor related striatal disorders. To investigate this possibility we evaluated if CBD would prevent catalepsy induced by drugs with distinct pharmacological mechanisms. The catalepsy test is largely used to investigate impairments of motor function caused by interference on striatal function. Male Swiss mice received acute pretreatment with CBD (5, 15, 30 or 60mg/kg, ip) 30min prior to the D2 receptor antagonist haloperidol (0.6mg/kg), the non-selective nitric oxide synthase (NOS) inhibitor L-nitro-N-arginine (L-NOARG, 80mg/kg) or the CB1 receptor agonist WIN55,212-2 (5mg/kg). The mice were tested 1, 2 or 4h after haloperidol, L-NOARG or WIN55,212-2 injection. These drugs significantly increased catalepsy time and this effect was attenuated dose-dependently by CBD. CBD, by itself, did not induce catalepsy. In a second set of experiments the mechanism of CBD effects was investigated. Thirty minutes before CBD (30mg/kg) the animals received the 5-HT1A receptor antagonist WAY100635 (0.1mg/kg). The anticataleptic effect of CBD was prevented by WAY100635. These findings indicate that CBD can attenuate catalepsy caused by different mechanisms (D2 blockade, NOS inhibition and CB1 agonism) via 5-HT1A receptor activation, suggesting that it could be useful in the treatment of striatal disorders.


Asunto(s)
Cannabidiol/uso terapéutico , Catalepsia/metabolismo , Catalepsia/prevención & control , Antagonistas de Dopamina/toxicidad , Receptor de Serotonina 5-HT1A/metabolismo , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Animales , Cannabidiol/farmacología , Catalepsia/inducido químicamente , Relación Dosis-Respuesta a Droga , Masculino , Ratones , Óxido Nítrico Sintasa/antagonistas & inhibidores , Agonistas del Receptor de Serotonina 5-HT1/farmacología , Agonistas del Receptor de Serotonina 5-HT1/uso terapéutico
16.
Eur Neuropsychopharmacol ; 23(10): 1329-35, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23141373

RESUMEN

The neurotransmitter serotonin (5-hydroxytryptamine; 5-HT) exerts a multifaceted function in the modulation of information processing, through the activation of multiple receptor families. In particular, stimulation of 5-HT(1A) and 5-HT(2A) receptors leads to sensorimotor gating impairments and perceptual perturbations. Previous evidence has shown that chronic deprivation of L-tryptophan (TRP), the precursor of 5-HT, results in marked reductions of 5-HT brain levels, as well as neuroplastic alterations in 5-HT(1A) and 5-HT(2A) expression and/or signaling. Building on these premises, in the present study we tested whether a prolonged TRP deprivation may differentially impact the roles of these receptors in the regulation of the prepulse inhibition (PPI) of the acoustic startle reflex, a dependable index of gating. Male Sprague-Dawley rats were fed for 14 days with either a regimen with negligible TRP content (TR-) or the same diet supplemented of TRP (TR+). At the end of this schedule, rats were treated with the prototypical 5-HT(1A) receptor agonist 8-OH-DPAT (62.5-250 µg/kg, subcutaneous, s.c.) or the 5-HT2 receptor agonist DOI (0.25-1 mg/kg, s.c.). Notably, the PPI deficits induced by 8-OH-DPAT in TR- rats were significantly milder than those observed in their TR+ counterparts; these effects were fully prevented by the 5-HT(1A) antagonist WAY-100135 (10 mg/kg, intraperitoneal). Conversely, TRP deprivation did not affect the PPI-disrupting properties of DOI. These findings suggest that prolonged 5-HT depletion attenuates the influence of 5-HT(1A), but not 5-HT2 receptors on sensorimotor gating, confirming the distinct mechanisms of these two targets in PPI regulation.


Asunto(s)
Discinesia Inducida por Medicamentos/dietoterapia , Trastornos Neurológicos de la Marcha/dietoterapia , Receptor de Serotonina 5-HT1A/metabolismo , Filtrado Sensorial/efectos de los fármacos , Neuronas Serotoninérgicas/efectos de los fármacos , Agonistas del Receptor de Serotonina 5-HT1/toxicidad , Triptófano/deficiencia , Estimulación Acústica , Animales , Conducta Animal/efectos de los fármacos , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Trastornos Neurológicos de la Marcha/inducido químicamente , Masculino , Proteínas del Tejido Nervioso/agonistas , Proteínas del Tejido Nervioso/antagonistas & inhibidores , Proteínas del Tejido Nervioso/metabolismo , Inhibición Neural/efectos de los fármacos , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Receptor de Serotonina 5-HT1A/química , Receptores de Serotonina 5-HT2/química , Receptores de Serotonina 5-HT2/metabolismo , Reflejo de Sobresalto/efectos de los fármacos , Neuronas Serotoninérgicas/metabolismo , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Agonistas del Receptor de Serotonina 5-HT2/toxicidad , Triptófano/antagonistas & inhibidores
17.
Chem Biol Drug Des ; 81(3): 389-98, 2013 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-23121934

RESUMEN

A series of 3,4,5-trimethoxycinnamic acid derivatives was prepared and evaluated for antinarcotic effects on morphine dependence in mice and binding affinities on serotonergic receptors. The key synthetic strategies involve generation of ketones 6-7, esters 9-12 through condensation reaction, and amides 13-19 via coupling reaction using 1-hydroxybenzotriazole/ethyl(dimethylaminopropryl)carbodiimide system in high yield. We found that the naloxone-induced morphine withdrawal syndrome was significantly suppressed by new synthetic 3,4,5-trimethoxycinnamic acid derivatives (20 mg/kg/day). Most of 3,4,5-trimethoxycinnamic acid derivatives were found to have high affinity to 5-HT(1A) receptor. The naloxone-induced morphine withdrawal syndrome was attenuated by (+)8-OH-DPAT (0.1 mg/kg/day, i.p.), a 5-HT(1A) receptor agonist. In cortical neuronal cells, (+)8-OH-DPAT (1 µM) produced an elevation of the pERK 1/2 expression, and the elevated pERK levels were inhibited by WAY 100635, a 5-HT(1A) receptor-specific antagonist. Interestingly, the pERK levels were increased by the 3,4,5-trimethoxycinnamic acid derivatives and the derivatives-mediated changes in pERK levels were blocked by the WAY 100635. These results suggested that new synthetic 3,4,5-trimethoxycinnamic acid derivatives have a potential antinarcotic effect through acting as a 5-HT(1A) receptor agonist in mice.


Asunto(s)
Analgésicos/síntesis química , Cinamatos/química , Analgésicos/química , Analgésicos/farmacología , Animales , Conducta Animal/efectos de los fármacos , Células CHO , Células Cultivadas , Cinamatos/síntesis química , Cinamatos/farmacología , Cricetinae , Cricetulus , Evaluación Preclínica de Medicamentos , Ratones , Ratones Endogámicos C57BL , Proteína Quinasa 1 Activada por Mitógenos/antagonistas & inhibidores , Proteína Quinasa 1 Activada por Mitógenos/metabolismo , Proteína Quinasa 3 Activada por Mitógenos/antagonistas & inhibidores , Proteína Quinasa 3 Activada por Mitógenos/metabolismo , Piperazinas/química , Piperazinas/farmacología , Unión Proteica , Piridinas/química , Piridinas/farmacología , Receptor de Serotonina 5-HT1A/química , Receptor de Serotonina 5-HT1A/metabolismo , Receptores de Serotonina/química , Receptores de Serotonina/metabolismo , Antagonistas del Receptor de Serotonina 5-HT1/síntesis química , Antagonistas del Receptor de Serotonina 5-HT1/química , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Transducción de Señal/efectos de los fármacos
18.
J Ethnopharmacol ; 143(2): 533-9, 2012 Sep 28.
Artículo en Inglés | MEDLINE | ID: mdl-22819689

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: Yokukansan, a traditional Japanese medicine (Kampo), has been reported in the treatment of behavioral and psychological symptoms of dementia (BPSD) such as aggression, anxiety and depression in patients with Alzheimer's disease and other forms of senile dementia. AIMS OF THE STUDY: In the present study, we investigated the anxiolytic effects of yokukansan on anxiety-related behaviors in rats that have experienced aversive stress. MATERIALS AND METHODS: We used male Wistar/ST rats which received an electrical footshock as aversive stress. Yokukansan at a dose of 1.0 g/kg was administered orally once a day for 14 or 16 day before behavioral tests. To evaluate the anxiolytic effects, we used the contextual fear conditioning (CFC) test and elevated plus-maze (EPM) test. And we also investigated effects of yokukansan on locomotor activity in the Open-field (OF) test and on the change in plasma corticosterone after CFC stress, in rats that had experienced footshock stress. RESULTS: In the CFC test, rats that had experienced footshock showed significant freezing behavior on re-exposure to the box 14 day after footshock stress. Yokukansan significantly suppressed freezing behavior in the CFC test. In the EPM test on the 16th day after the CFC test, yokukansan significantly increased the time spent in open arms after footshock stress compared to control rats. However, repeated administration of yokukansan on the 14th day did not affect the decrease in locomotor activity and the increase in plasma corticosterone by re-exposure to the box 14 day after footshock stress in the OF test and determination of serum corticosterone, respectively. These anxiolytic effects by yokukansan were antagonized by WAY-100635, a selective 5-HT(1A) receptor antagonist, in the CFC test, but not the EPM test. Furthermore, 5-HT(1A) receptor agonist buspirone significantly suppressed freezing behavior in the CFC test; however, buspirone induced no change in the time spent in open arms in the EPM test. CONCLUSION: These findings suggested that yokukansan has anxiolytic effects on anxiety-like behaviors induced by both innate fear and memory-dependent fear. In particular, yokukansan produced anxiolytic effects via 5-HT(1A) receptors in memory-dependent fear induced by aversive stress. Furthermore, yokukansan could be useful as one of the therapeutic drugs for the treatment of anxiety disorders and various mental disorders that have comorbid anxiety.


Asunto(s)
Ansiolíticos/uso terapéutico , Ansiedad/tratamiento farmacológico , Medicamentos Herbarios Chinos/uso terapéutico , Receptor de Serotonina 5-HT1A/fisiología , Animales , Ansiolíticos/farmacología , Ansiedad/sangre , Ansiedad/fisiopatología , Conducta Animal/efectos de los fármacos , Buspirona/farmacología , Corticosterona/sangre , Medicamentos Herbarios Chinos/farmacología , Miedo/efectos de los fármacos , Japón , Masculino , Medicina Tradicional de Asia Oriental , Actividad Motora/efectos de los fármacos , Piperazinas/farmacología , Piridinas/farmacología , Ratas , Ratas Wistar , Agonistas del Receptor de Serotonina 5-HT1/farmacología , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Estrés Fisiológico
19.
Br J Anaesth ; 109(2): 245-52, 2012 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-22628394

RESUMEN

BACKGROUND: Although acupuncture analgesia is well documented, its mechanisms have not been thoroughly clarified. We previously showed that electroacupuncture (EA) activates supraspinal serotonin- and norepinephrine-containing neurones that project to the spinal cord. This study investigates the involvement of spinal alpha(2)-adrenoceptors (α2-ARs) and 5-hydroxytryptamine (serotonin) receptors (5-HTRs) in EA effects on an inflammatory pain rat model. METHODS: Inflammatory hyperalgesia was induced by injecting complete Freund's adjuvant (CFA, 0.08 ml) into the plantar surface of one hind paw and assessed by paw withdrawal latency (PWL) to a noxious thermal stimulus. The selective α2a-AR antagonist BRL-44408, α2b-AR antagonist imiloxan hydrochloride, 5-HT2B receptor (5-HT2BR) antagonist SB204741, 5-HT3R antagonist LY278584, or 5-HT1AR antagonists NAN-190 hydrobromide, or WAY-100635 were intrathecally administered 20 min before EA or sham EA, which was given 2 h post-CFA at acupoint GB30. RESULTS: EA significantly increased PWL compared with sham [7.20 (0.46) vs 5.20 (0.43) s]. Pretreatment with α2a-AR [5.35 (0.45) s] or 5-HT1AR [5.22 (0.38) s] antagonists blocked EA-produced anti-hyperalgesia; α2b-AR, 5-HT2BR, and 5-HT3R antagonist pretreatment did not. Sham plus these antagonists did not significantly change PWL compared with sham plus vehicle, indicating that the antagonists had little effect on PWL. Immunohistochemical staining demonstrated that α2a-ARs are on primary afferents and 5-HT1ARs are localized in N-methyl-d-aspartic acid (NMDA) subunit NR1-containing neurones in the spinal dorsal horn. CONCLUSIONS: The data show that α2a-ARs and 5-HT1ARs are involved in the EA inhibition of inflammatory pain and that the NMDA receptors are involved in EA action.


Asunto(s)
Electroacupuntura/métodos , Hiperalgesia/prevención & control , Receptores Adrenérgicos alfa 2/fisiología , Receptores de Serotonina 5-HT1/fisiología , Antagonistas de Receptores Adrenérgicos alfa 2/farmacología , Animales , Modelos Animales de Enfermedad , Adyuvante de Freund , Calor , Hiperalgesia/inducido químicamente , Hiperalgesia/metabolismo , Masculino , Dimensión del Dolor/métodos , Ratas , Ratas Sprague-Dawley , Tiempo de Reacción/efectos de los fármacos , Tiempo de Reacción/fisiología , Receptores Adrenérgicos alfa 2/metabolismo , Receptores de Serotonina 5-HT1/metabolismo , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Médula Espinal/metabolismo
20.
J Ethnopharmacol ; 141(3): 872-7, 2012 Jun 14.
Artículo en Inglés | MEDLINE | ID: mdl-22472106

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal applications of Pimenta pseudocaryophyllus infusion as a diuretic and aphrodisiac agent as well as tranquilizer in the form of tea for the treatment of emotional tension in Brazilian folk medicine has been in practice since time immemorial. Despite its popular therapeutic acceptance and claims, there are scanty scientific reports to corroborate its central biological activities. AIM: To characterize anxiolytic-like effect of the dichloromethane fraction (DF) obtained from ethanolic leaf extract of the Pimenta pseudocaryophyllus and identify mechanisms of action involved while seeking to support its popular use as a soothing agent. MATERIAL AND METHODS: Mice (25-35 g) were treated orally with DF obtained from ethanolic leaf extract of Pimenta pseudocaryophyllus and were submitted to light-dark box (LDB) and elevated plus maze (EPM) tests. Different groups of mice were treated with flumazenil and NAN-190 to identify mechanisms of action involved in the anxiolytic-like effect of DF. RESULTS: Treatment with DF increased number of transitions and time spent in the light compartment of the LDB while the time spent and numbers of entries in the open arm of the LCE were significantly increased. Pre-treatment of the animal with flumazenil (2 mg/kg, i.p.--competitive antagonist of benzodiazepine site of GABA(A) receptor) did not block this effect, thereby excluding participation of benzodiazepine site of the GABA(A) receptor. However, anxiolytic-like effect of DF was reversed by pre-treatment with NAN-190 (0.5 mg/kg, i.p.--an antagonist of the 5-HT(1A) receptor) thereby suggesting involvement of 5-HT(1A) receptor. The thin layer chromatography and high-performance liquid chromatography analysis indicated the predominance of (E)-methyl isoeugenol and oleanolic acid in DF. CONCLUSION: These results support the popular use of Pimenta pseudocaryophyllus as a calming agent and suggest the involvement of 5-HT(1A) receptor.


Asunto(s)
Ansiolíticos/farmacología , Ansiedad/fisiopatología , Pimenta , Extractos Vegetales/farmacología , Receptor de Serotonina 5-HT1A/fisiología , Animales , Ansiolíticos/uso terapéutico , Ansiedad/tratamiento farmacológico , Conducta Animal/efectos de los fármacos , Modelos Animales de Enfermedad , Etanol/química , Flumazenil/farmacología , Moduladores del GABA/farmacología , Masculino , Cloruro de Metileno/química , Ratones , Piperazinas/farmacología , Extractos Vegetales/uso terapéutico , Hojas de la Planta , Antagonistas del Receptor de Serotonina 5-HT1/farmacología , Solventes/química
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