Ecological risk assessment of Central Asian mining sites: application of the ERICA assessment tool.

Recent field expeditions to Central Asian mining sites have provided a wealth of data on radionuclide and metal concentrations in environmental media. In this paper the ERICA assessment tool was used to provide an assessment of the potential doses to non-human biota at the various sites. The aim was to identify the most exposed organism types and the radionuclides giving rise to the greatest doses. The measured media and biota activity concentrations were also used to compare site-specific Kds and CRs with default tool parameters. At all terrestrial sites, the maximum doses (up to ca. 600 µGy/h) were seen in lichens and bryophytes, with concentrations of radium in soils dominating the assessments. Internal alpha dose from (226)Ra was the biggest dose contributor, representing between 72 and 97% of the total dose, with U isotopes as the next most significant contributor. For aquatic organisms the highest calculated doses were obtained for aquatic plants (ca. 100 µGy/h), followed by molluscs, crustacean, zooplankton and insect larva, based on at site environmental media data. For aquatic plants, the internal alpha doses from uranium isotopes dominated the dose at most of the sites, hence the highest doses were seen at sites with the highest U concentrations. While the measured and modelled concentrations were usually in reasonable agreement, particularly for U and Ra in terrestrial plants, there were some differences, most notably for U and Po in the aquatic environment. Modelled concentrations of U in aquatic plants tended to be higher than those measured in site samples; while Po in fish was greater than modelled concentrations. Furthermore, not all the organisms listed in the ERICA tool had been sampled at the sites. Nevertheless, the assessment results should be of great benefit in identifying priority areas for future field studies.

Assessment of the radiological impact of gamma and radon dose rates at former U mining sites in Central Asia.

An assessment of the radiological situation due to exposure to gamma radiation, radon and thoron was carried out at selected former uranium mining and processing sites in the Central Asian countries of Kazakhstan, Kyrgyzstan, Uzbekistan and Tajikistan. Gamma dose rate measurements were made using various field instruments and radon/thoron measurements were carried out using discriminative radon ((222)Rn)/thoron ((220)Rn) solid state nuclear track detectors (SSNTD). The detectors were exposed for an extended period of time, including at least three seasonal periods in a year, in different outdoor and indoor public and residential environments at the selected uranium legacy sites. The results showed that gamma, Rn and Tn doses were in general low, which consequently implies a low/relatively low radiological risk. The major radiation hazard is represented by abandoned radioactive filtration material that was being used as insulation by some Minkush residents (Kyrgyzstan) for a longer period of time. Annual radiation doses of several hundred mSv could be received as a consequence of using this material domestically. In addition, the gamma and Rn/Tn dose rates at Digmai, Tajikistan, could reach values of several 10 mSv/a. The doses of ionizing radiation deriving from external radiation (gamma dose rate), indoor radon and thoron with their short-lived progenies in several cases exceeded the recommended annual effective dose threshold level of 10 mSv. At none of the sites investigated did the individual annual effective doses exceed 30 mSv, the internationally recommended value for considering intervention. Current doses of ionizing radiation do not represent a serious hazard to the health of the resident public, but this issue should be adequately addressed to further reduce needless exposure of the resident public to ionizing radiation.

Micro-analytical characterisation of radioactive heterogeneities in samples from Central Asian TENORM sites.

The present work focuses on the use of micro-analytical techniques to demonstrate the heterogeneous distribution of radionuclides and metals in soils collected at Former Soviet Union mining sites in Central Asia. Based on digital autoradiography, radionuclides were heterogeneously distributed in soil samples collected at the abandoned uranium mining sites Kurday, Kazakhstan, Kadji Sai, Kyrgyzstan and Taboshar, Tajikistan. Using electron microscopy interfaced with X-ray microanalysis submicron - mm-sized radioactive particles and rock fragments with U, As, Se and toxic metals on the surfaces were identified in Kurday and Kadji Sai samples. Employing scanning and tomographic (3D) synchrotron radiation based micro-X-ray fluorescence (µ-SRXRF) and synchrotron radiation based micro-X-ray diffraction (µ-SRXRD) allowed us to observe the inner structure of the particles without physical sectioning. The distribution of elements in virtual crosssections demonstrated that U and a series of toxic elements were rather heterogeneously distributed also within individual radioactive TENORM particles. Compared to archived data, U in Kadji Sai particles was present as uraninite (U4O9+y or UO2+x) or Na-zippeite ((Na4(UO2)6[(OH)10(SO4)3]·4H2O), i.e. U minerals with very low solubility. The results suggested that TENORM particles can carry substantial amount of radioactivity, which can be subject to re-suspension, atmospheric transport and water transport. Thus, the potential radioecological and radioanalytical impact of radioactive particles at NORM and TENORM sites worldwide should be taken into account. The present work also demonstrates that radioecological studies should benefit from the use of advanced methods such as synchrotron radiation based techniques.

On the origin of the woody buckwheat Fagopyrum tibeticum (=Parapteropyrum tibeticum) in the Qinghai-Tibetan Plateau.

Here we tested whether 'insular woodiness', a striking evolutionary pattern that commonly occurs on islands, has also appeared in QTP continental endemics. Parapteropyrum, a monotypic shrubby genus occurring in the central QTP, has been previously placed in the tribe Atraphaxideae of the family Polygonaceae, while all the other woody species of this tribe mainly occur in western and central Asia. We studied sequence variations of nuclear ITS (internal transcribed spacer) and cp (chloroplast) DNA (rbcL and accD) of this genus and the other ten genera. The constructed phylogenies based on ITS, cpDNA or a combination of both datasets, suggest that the woody Parapteropyrum is nested within and most likely evolved from the herbaceous Fagopyrum. We propose that the large-scale uplift of the QTP not only promoted continental species radiation, but also the secondary feature of woodiness in a few herbaceous lineages in response to strong selection pressures, similar to those acting on island flora. In addition, the confirmation of Parapteropyrum within Fagopyrum highlights its potential use as a new, perennial source of buckwheat.

The Golden Crescent and HIV/AIDS in Central Asia: deadly interactions.

Afghanistan has become the world's largest producer of illicit opiates. Opium and its derivative heroin are widespread substances of use, abuse and dependency in Central Asia. The region is currently undergoing expanding HIV epidemics driven largely by needle sharing among people who use drugs, in contexts where public health interventions to reduce the harms associated with substance use are limited by policy, law and legalistic and repressive approaches to drug users. Evidence-based approaches to drug treatment are lacking or limited in multiple states. Urgent reform is needed. The massive volumes of Afghan's illicit opiate exports are having serious impacts on the health of the region.

Paleoneurosurgical aspects of Proto-Bulgarian artificial skull deformations.

Paleoneurosurgery represents a comparatively new developing direction of neurosurgery dealing with archaeological skull and spine finds and studying their neurosurgical aspects. Artificial skull deformation, as a bone artifact, naturally has been one of the main paleoneurosurgical research topics. Traditionally, the relevant neurosurgical literature has analyzed in detail the intentional skull deformations in South America's tribes. However, little is known about the artificial skull deformations of the Proto-Bulgarians, and what information exists is mostly due to anthropological studies. The Proto-Bulgarians originated from Central Asia, and distributed their skull deformation ritual on the Balkan Peninsula by their migration and domination. Proto-Bulgarian artificial skull deformation was an erect or oblique form of the anular type, and was achieved by 1 or 2 pressure bandages that were tightened around a newborn's head for a sufficiently long period. The intentional skull deformation in Proto-Bulgarians was not associated with neurological deficits and/or mental retardation. No indirect signs of chronic elevated intracranial pressure were found on the 3D CT reconstruction of the artificially deformed skulls.

[Anthropology and "crisis in medicine": The pathologist M. Kuczynski-Godard (1890-1967) and the indigenous peoples of Central Asia and Peru]. / Antropología y "crisis de la medicina": el patólogo M. Kuczynski-Godard (1890-1967) y las poblaciones nativas en Asia Central y Perú.

This article examines the work of the German-Peruvian physician Max Kuczynski/Máxime Kuczynski-Godard (Berlin 1890-Lima 1967) in rural areas of Central Asia (1924-26) and Peru (1938-48). The main focus of the text is on the scientific approach behind the specific interest of this pathologist in disease and health issues among native populations. Kuczynski's theoretical considerations are analyzed in the context of the wide controversies within the German medical community around a "crisis in medicine" when he was professor at Berlin University during the interwar years. Accordingly, his determination to leave the laboratory and to shift research and healthcare practice closer to rural populations proves to be the expression of profound epistemological and ethical considerations.

Plant extracts from central Asia showing antiinflammatory activities in gene expression assays.

Plant natural products remain a good resource for the discovery of novel pharmaceuticals. A mouse macrophage-based quantitative, reverse transcription polymerase chain reaction (qRT-PCR) system was optimized to screen plant extracts for antiinflammatory activities using three well known genetic markers of inflammation. Plants used for extraction were taxonomically identified and vouchered species from two Central Asian countries, Uzbekistan and Kyrgyzstan, collected through the International Cooperative Biodiversity Groups (ICBG) program. The mRNA expression of cyclooxygenase-2, interleukin 1beta and inducible nitric oxide synthase genes in RAW macrophages was determined quantitatively in response to treatment with plant extracts applied at 100 microg/mL. The screening of 1000 extracts from 449 plant species belonging to 68 plant families resulted in 75 extracts (7.5%) showing strong (75% or higher inhibition) activity against at least one target gene. Many extracts showed qualitative and quantitative differences in the levels of activities against each target gene. Extracts identified from this screen were able to reduce inflammatory symptoms in vivo, thereby validating the screening approach.

Tracking coverage on the silk road: time to turn theory into practice.

Scaling up coverage of programs that effectively reduce the spread of HIV among vulnerable populations, including injecting drug users (IDUs), sex workers (SWs), and men who have sex with men (MSM), is a critically important issue for many countries today. However, in addition to the lack of a commonly accepted definition of coverage, there are currently no universally accepted standards, methodologies, or tools to track coverage among these groups. Globally, most programs working to prevent HIV among vulnerable populations are not using monitoring & evaluation (M&E) systems that accurately track numbers of clients and frequency of contact with those clients. Nor do most programs have targets on the frequency of contact needed to effectively promote healthy behaviours. This article presents a narrative of how one program in Central Asia developed a simple M&E system to track the extent and frequency of contacts among clients. The system uses a simple and anonymous "Unique Identifier Code" (UIC) that is assigned to each client and recorded into a simple database to track the client's interaction with the program. The system allows program managers to track numbers of clients served and at what frequency and to better monitor progress towards goals. The data produced by the UIC system, when compared against HIV and sexually transmitted infection (STI) sentinel surveillance data by site, allows programs to test theorized definitions of the quantity of coverage needed to reduce the risk behaviours that spread HIV among vulnerable populations. Such systems can then provide urgently needed data to help national HIV/AIDS programs understand current coverage levels and gaps in coverage that need to be filled in order to reduce the spread of HIV. Such a system provides valuable data to enable decision makers to make evidence-based decisions on how to allocate resources to reach sufficient coverage to reduce the spread of HIV among populations most at risk of HIV.

Evaluation of the potential effectiveness of wheat flour and salt fortification programs in five Central Asian countries and Mongolia, 2002-2007.

BACKGROUND: A project for universal salt iodation with potassium iodate and wheat flour fortification with a vitamin-mineral premix was implemented in Azerbaijan, Kazakhstan, Kyrgyzstan, Mongolia, Tajikistan, and Uzbekistan between 2002 and 2007. OBJECTIVE: To determine the potential effectiveness of the food fortification programs in improving the micronutrient status of selected families in a sentinel population in each country. METHODS: An area was selected in each country in a sentinel population expected to have early access to iodated salt and fortified wheat flour. Within this area, an average of 40 families with a woman of reproductive age and two children between 2 and 15 years old were sampled at baseline. All the rounds of the study were carried out in women and children in the same households. Hemoglobin, serum ferritin, folic acid, and urinary iodine excretion were analyzed at baseline, one year, and three years later. RESULTS: In the third round in 2007 significant increases were observed in the average levels of blood hemoglobin, serum ferritin and folic acid, and urinary iodine. Corresponding decreases in the prevalence of anemia and increases in serum ferritin levels, folic acid, and iodine were found. CONCLUSIONS: Salt and wheat flour fortification resulted in a significant improvement in the micronutrient status of children and women living in sentinel households in the countries participating in the Asian Development Bank project. Sentinel studies were a cost-effective way of determining potential national effectiveness.

Critical methods in Tibetan medical histories.

This paper addresses the development of scholastic medical traditions in Tibet through an extension of lists of physicians. I consider the debates that such lists and their accompanying narratives engender for Tibetan historians and reflect on the contributions they make to the identity of the medical tradition. By examining the structure and content of classificatory methods in medical histories, I argue that temporally organized lists document the place of medicine across time, geographically organized lists document the reach of medical knowledge across space, and thematically organized lists document the intertwining of medical knowledge and skill with other aspects of intellectual and civil life. In making these lists, medical historians paint a portrait of the Tibetan medical tradition that evokes connections to Buddhism and the strength and cosmopolitanism of the imperial period. Medical histories thus emphasize a picture of Tibet in the broader context of Asia- a Tibet whose empire lives on culturally or intellectually, if not militarily.

Protective effects of Munziq and Mushil of abnormal Savda to mitochondrial oxidative damage.

Munziq and Mushil of Abnormal Savda are traditional Uighur herbal medicinal products, which could have antioxidant properties protecting mitochondria against oxidative damage. Mitochondria were isolated from rat livers. A FeSO4/VitC hydroxyl radical-generating system was used to induce mitochondrial oxidative damage. Alterations in mitochondrial membrane structure were observed by electron microscopy, and mitochondrial superoxide dismutase (SOD) activity, malondialdehyde (MDA) level and Ca2+-Mg2+-ATPase activity were measured. Muziq or Mushil were added, at concentrations ranging from 10(-5) to 10(-1) g/mL. Mitochondrial membrane structure was damaged after exposure to hydroxyl radical; mitochondrial SOD and Ca2+-Mg2+-ATPase activities decreased (by 80 and 55%, respectively, both P < 0.01), and MDA level increased 4.6-fold (P < 0.01). Munziq and Mushil protected mitochondrial membranes from structural damage. They inhibited the changes in mitochondrial functions in a dose-dependent manner. At the highest concentrations, values were equal to initial normal values. Munziq and Mushil of Abnormal Savda can reduce the oxidative damage induced by hydroxyl radical and protect the mitochondrial membrane structure and its functions.

Treatments and perceptions of epilepsy in Kashmir and the United States: a cross-cultural analysis.

Treatments and perceptions of epilepsy have been found to vary across cultures. This study draws on a comparison of two patient samples, one from the United States (n=28), the other from Kashmir (n=29), to gauge the similarities and differences in social perceptions of epilepsy, attitudes toward conventional and alternative treatments, practice of conventional and alternative treatments, and selected quality-of-life issues. While both the Kashmiri and American patients interviewed were prescribed a similar regimen of traditional antiepileptic drugs, a wider range of drugs and treatments were available to and used by the latter. The use of adjunctive spiritual therapies was more prevalent in the Kashmiri sample, and the use of alternative, nonpharmacological therapies was more prevalent in the American sample. Quality of life for the Kashmiri patients sampled was found to be poorer in terms of educational and occupational opportunities, feelings of stigmatization, and openness with others about the illness. Although the two patient populations interviewed differed in their access to resources and approaches to the disorder, both samples were found to be similar overall in many attitudes and practices relating to epilepsy and its treatment.

New sesquiterpenes from Ferula ferulaeoides (Steud.) Korovin. VI. Isolation and identification of three new dihydrofuro[2,3-b]chromones.

Three novel 2-prenyl-dihydrofurochromone-type sesquiterpenoid derivatives, 2,3-dihydro-7-hydroxy-2S*,3R*-dimethyl-2-[4,8-dimethyl-3(E),7-nonadienyl]-furo[2,3-b]chromone, 2,3-dihydro-7-hydroxy-2S*,3R*-dimethyl-2-[4-methyl-5-(4-methyl-2-furyl)-3(E),7-pentenyl]-furo[2,3-b]chromone, and 2,3-dihydro-7-hydroxy-2R*,3R*-dimethyl-2-[4-methyl-5-(4-methyl-2-furyl)-3(E),7-pentenyl]-furo[2,3-b]chromone, were isolated from the roots of Ferula ferulaeoides. The structures were established by comprehensive spectral analysis. The biosynthetic pathway leading to these 2-prenyl-dihydrofurochromone-type sesquiterpenoids is proposed based on their structures.

Anemia prevention and control in four central Asian republics and Kazakhstan.

Kazakhstan and the central Asian republics of Uzbekistan, the Kyrgyz Republic, Tajikistan and Turkmenistan have developed anemia prevention and control (APC) policies based on multiple interventions, including education and promotion, oral supplementation of high risk groups and fortification of wheat flour with iron and other micronutrients. These national strategies are aimed at reducing the prevalence of anemia and iron deficiency among young children and women of child-bearing age. Strategy development has been assisted by funding and technical assistance from the United Nations Children's Fund (UNICEF) with additional technical support from the International Nutrition Foundation, the United Nations University and various national institutions. These countries have been among the most advanced in adopting national strategies that include multiple interventions in an overall package, and national interest in APC remains high. However, reviews of APC activities conducted in 2001 suggests the need for modification and enhancement of current efforts and for a shift to national-level actions if these countries are to progress toward current and future goals. Increased commitment and determination, by both national groups and international organizations, are required to achieve and sustain improvement in micronutrient nutrition.

[The "hornet spider" of Plinius]. / Zur "Hornissen-Spinne" des Plinius.

Pliny the Elder describes in his 'Natural History' XXIX 86 a species of spider dangerous for human beings and looking like a hornet without wings. This description corresponds to Solipugae of the genus Karschia, living in arid areas of Central Asia. These animals are not venomous but can cause harm by their powerful bite and in some cases by a following inflammation of the wound. On the contrary, an animal called "wasp-like", mentioned by Nikander of Kolophon ('Theriaca', v. 738-746) and often regarded to be the same creature, seems to be a typical venomous spider which causes an acute intoxication by its bite.

The role of fermented milk in complementary feeding of young children: lessons from transition countries.

Probiotic bacteria are used for production of fermented dairy products. The use of probiotic bacteria has the potential to replenish the natural intestinal flora of the body. These bacteria competitively inhibit the growth and colonization of pathogenic bacteria. Breastmilk is the best food for babies, also from a probiotic point of view. Human milk, in fact, contains many substances that stimulate the growth of bifidobacteria in vitro and in the small intestine of infants. Improvement of lactose digestion and avoidance of symptoms of intolerance in lactose malabsorbers are the most profoundly studied health-relevant effects of fermented milk. In fact fermented milks are nutritionally similar to unfermented milk, except that some of lactose is broken down to glucose and galactose. The role of fermented milk in complementary feeding and in particular for the prevention of anaemia is an innovative theme, recently focused. Iron deficiency in infants and young children is widespread and has serious consequences for child health. Prevention of iron deficiency should therefore be given high priority. The too-early introduction of unmodified cow's milk and milk products is an important nutritional risk factors for the development of iron-deficiency anaemia. Fermented milks represent an excellent source of nutrients such as calcium, protein, phosphorus and riboflavin. During the fermentation of milk, lactic acid and other organic acids are produced and these increase the absorption of iron. If fermented milk is consumed at mealtimes, these acids are likely to have a positive effect on the absorption of iron from other foods.