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1.
Artículo en Inglés | MEDLINE | ID: mdl-34731744

RESUMEN

BACKGROUND: Coffee is one of the most frequently consumed beverages worldwide. Research on effects of coffee drinking has focused on caffeine; however, coffee contains myriad biochemicals that are chemically unrelated to caffeine, including 3,4-dihydroxyphenyl compounds (catechols) such as caffeic acid and dihydrocaffeic acid (DHCA). OBJECTIVE: This prospective within-subjects study examined effects of drinking caffeinated or decaffeinated coffee on plasma free (unconjugated) catechols measured by liquid chromatography with series electrochemical detection (LCED) after batch alumina extraction. To confirm coffee-related chromatographic peaks represented catechols, plasma was incubated with catechol-O-methyltransferase and S-adenosylmethionine before the alumina extraction; reductions in peak heights would identify catechols. METHODS: Ten healthy volunteers drank 2 cups each of caffeinated and decaffeinated coffee on separate days after fasting overnight. With subjects supine, blood was drawn through an intravenous catheter up to 240 min after coffee ingestion and the plasma assayed by alumina extraction followed by LCED. RESULTS: Within 15 min of drinking coffee of either type, >20 additional peaks were noted in chromatographs from the alumina eluates. Most of the coffee-related peaks corresponded to free catechols. Plasma levels of the catecholamines epinephrine and dopamine increased with both caffeinated and decaffeinated coffee. Levels of other endogenous catechols were unaffected. Plasma DHCA increased bi-phasically, in contrast with other coffee-related free catechols. INTERPRETATION: Drinking coffee-whether caffeinated or decaffeinated-results in the rapid appearance of numerous free catechols in the plasma. These might affect the disposition of circulating catecholamines. The bi-phasic increase in plasma DHCA is consistent with production by gut bacteria.


Asunto(s)
Cafeína/análisis , Catecoles/sangre , Café/metabolismo , Adulto , Ácidos Cafeicos/sangre , Cafeína/metabolismo , Café/química , Femenino , Humanos , Masculino , Plasma/química , Estudios Prospectivos , Adulto Joven
2.
JCI Insight ; 6(3)2021 02 08.
Artículo en Inglés | MEDLINE | ID: mdl-33373329

RESUMEN

Ginger is known to have antiinflammatory and antioxidative effects and has traditionally been used as an herbal supplement in the treatment of various chronic diseases. Here, we report antineutrophil properties of 6-gingerol, the most abundant bioactive compound of ginger root, in models of lupus and antiphospholipid syndrome (APS). Specifically, we demonstrate that 6-gingerol attenuates neutrophil extracellular trap (NET) release in response to lupus- and APS-relevant stimuli through a mechanism that is at least partially dependent on inhibition of phosphodiesterases. At the same time, administration of 6-gingerol to mice reduces NET release in various models of lupus and APS, while also improving other disease-relevant endpoints, such as autoantibody formation and large-vein thrombosis. In summary, this study is the first to our knowledge to demonstrate a protective role for ginger-derived compounds in the context of lupus. Importantly, it provides a potential mechanism for these effects via phosphodiesterase inhibition and attenuation of neutrophil hyperactivity.


Asunto(s)
Catecoles/farmacología , Alcoholes Grasos/farmacología , Lupus Eritematoso Sistémico/tratamiento farmacológico , Lupus Eritematoso Sistémico/inmunología , Neutrófilos/efectos de los fármacos , Neutrófilos/inmunología , Animales , Anticuerpos Antifosfolípidos/biosíntesis , Síndrome Antifosfolípido/tratamiento farmacológico , Síndrome Antifosfolípido/inmunología , Síndrome Antifosfolípido/metabolismo , Catecoles/sangre , Catecoles/farmacocinética , Modelos Animales de Enfermedad , Trampas Extracelulares/efectos de los fármacos , Trampas Extracelulares/inmunología , Alcoholes Grasos/sangre , Alcoholes Grasos/farmacocinética , Femenino , Humanos , Técnicas In Vitro , Lupus Eritematoso Sistémico/metabolismo , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Neutrófilos/metabolismo , Inhibidores de Fosfodiesterasa 4/farmacología , Inhibidores de Fosfodiesterasa/sangre , Inhibidores de Fosfodiesterasa/farmacocinética , Inhibidores de Fosfodiesterasa/farmacología , Fitoterapia , Especies Reactivas de Oxígeno/metabolismo , Trombosis de la Vena/tratamiento farmacológico , Trombosis de la Vena/patología
3.
Phytomedicine ; 65: 153090, 2019 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-31557666

RESUMEN

BACKGROUND AND OBJECTIVE: Echinacea angustifolia DC. and Zingiber officinale Roscoe are two natural products with documented immunomodulatory activity, both able to modulate the expression of important immune-related genes. Thus, their use in combination seems to be particularly promising. In this context, we have considered the oral supplementation of a highly standardized lipophilic extract combining both above-mentioned phytocomplexes, formulated in attractive softgel capsules, with two objectives: on the one hand to study oral pharmacokinetic of main active extracts' components and on the other hand to examine the immunomodulation and anti-inflammatory properties by gene expression profiling. METHODS: Softgel capsules containing a combination of E. angustifolia DC. and Z. officinale Roscoe (5 mg and 25 mg, respectively) were given by oral administration to 10 healthy volunteers. The plasma concentrations of dodeca-2E,4E,8Z,10E/Z-tetraenoic isobutylamide (tetraene) for E. angustifolia DC., 6-gingerol and 6-shogaol (free and glucuronide) for Z. officinale Roscoe were determined by LC-MS analysis, and the pharmacokinetic analysis was performed. To understand the functional mechanisms responsible for the documented health benefits, we also examined the overall transcriptional remodeling induced in the peripheral blood mononuclear cells and performed an integrative functional analysis on the generated gene expression. RESULTS: All bioactive components were absorbed very rapidly, and their tmax were detected in plasma from 30 min to 1.40 h. The peak concentrations of tetraene, 6-gingerol, 6-shogaol and their glucuronide metabolites were 14.74, 5.66, 9.25, 29.2 and 22.24 ng/ml, respectively. Integrated analysis performed on the generated gene expression data highlighted immunomodulatory and anti-inflammatory effects similar to those exerted by hydrocortisone. CONCLUSION: These data demonstrated that the bioactive ingredients are highly and rapidly absorbed from softgel capsules containing the combination of the above-mentioned lipophilic extracts, providing evidence to support their immunomodulatory and anti-inflammatory properties. These data also help in defining the mechanistic pathways underlying the health benefits of these plant-derived bioactive compounds.


Asunto(s)
Echinacea/química , Factores Inmunológicos/farmacocinética , Extractos Vegetales/inmunología , Extractos Vegetales/farmacocinética , Zingiber officinale/química , Administración Oral , Antiinflamatorios/farmacocinética , Cápsulas , Catecoles/sangre , Alcoholes Grasos/sangre , Femenino , Perfilación de la Expresión Génica , Humanos , Leucocitos Mononucleares/efectos de los fármacos , Masculino , Extractos Vegetales/administración & dosificación
4.
Pharmacology ; 102(3-4): 154-160, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-30045035

RESUMEN

BACKGROUND: Protocatechuic aldehyde (PAL) and hydroxysafflor yellow A (HSYA) are 2 effective ingredients of Danhong Injection, which is extensively used for the clinical treatment of cardio-cerebrovascular diseases. This study aims to investigate the pharmacokinetic differences between single and combined medication of PAL and HSYA and analyze the interaction of the above effective components in hyperlipidemia rats. METHODS: Thirty male SD rats were randomly divided into the control group (n = 6) and the model group (n = 24). The hyperlipidemia model was established by feeding with superfatted forage. The successful model rats were then randomly divided into the PAL group (16 mg/kg), the HSYA group (10 mg/kg), and the combination group (16 mg/kg + 10 mg/kg). Administration through tail-vein, and orbital blood was sampled at different time points. The mass concentration of PAL and HSYA was determined by high performance liquid chromatography (HPLC-DAD). Analysis of pharmacokinetic parameters was conducted by using DAS 3.2.6 software and SPSS 19.0 statistical analysis software. RESULTS: According to the parameters of statistical moment of non-compartmental model, there was a significant difference in plasma clearance (CL) between the PAL group and the drug combination group (p < 0.01), as well as in the area under the first moment of the plasma concentration-time curve and the elimination half-life (t1/2) between the HSYA group and the drug combination group (p < 0.01) but no obvious differences about the blood concentration time curve area, the average dwell time (MRT), and the peak concentration (Cmax; p > 0.05). CONCLUSION: The combined medication of PAL and HSYA could increase the plasma CL significantly and have a great influence on the absorption of HSYA in rats with hyperlipidemia.


Asunto(s)
Benzaldehídos/farmacocinética , Catecoles/farmacocinética , Chalcona/análogos & derivados , Hiperlipidemias/metabolismo , Quinonas/farmacocinética , Animales , Anticoagulantes/sangre , Anticoagulantes/farmacocinética , Benzaldehídos/sangre , Cardiotónicos/farmacocinética , Catecoles/sangre , Chalcona/sangre , Chalcona/farmacocinética , Medicamentos Herbarios Chinos/farmacocinética , Hiperlipidemias/sangre , Hiperlipidemias/tratamiento farmacológico , Lípidos/sangre , Masculino , Quinonas/sangre , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley
5.
J Chromatogr Sci ; 56(2): 139-146, 2018 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-29186346

RESUMEN

The three analytes of the Traditional Chinese Medicine ZibuPiyin Recipe (ZBPYR), namely, liquiritin, protocatechuic aldehyde and rosmarinic acid, may synergistically play an important role in regulating memory and learning. However, the pharmacokinetic behaviors of these compounds after their co-administration remain unclear. To this end, a selective and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method was developed and validated in rat plasma for the study of these three major bioactive ingredients in ZBPYR. The analytes in the plasma samples were separated on a Shiseido Capcell core C18 column using bendrofluazide as an internal standard, with a gradient mobile phase system of acetonitrile-water containing 0.1% formic acid. Electrospray ionization in the negative-ion mode and multiple reaction monitoring were used to identify and quantify the three analytes. All of the calibration curves showed good linearity (r > 0.992) over the concentration range, with a lower limit of quantification of 5 ng/mL. The precision of the analytical method was evaluated by intra- and inter-day assays, and the percentage of relative standard deviation (SD) was within 15%. Satisfactory extraction efficiency (between 83.4 and 99.4%) and matrix effects (76.4-107.4) were obtained by liquid-liquid extraction. The pharmacokinetic results showed that the three bioactive ingredients were rapidly absorbed and had a short terminal half-life in rats after oral administration of ZibuPiyin recipe. This UPLC-MS-MS study method used in this study may be useful for assessing the pharmacokinetic characteristics of various compounds, which would be helpful in determining their clinical potential.


Asunto(s)
Benzaldehídos/farmacocinética , Catecoles/farmacocinética , Cromatografía Líquida de Alta Presión/métodos , Cinamatos/farmacocinética , Depsidos/farmacocinética , Medicamentos Herbarios Chinos/administración & dosificación , Flavanonas/farmacocinética , Glucósidos/farmacocinética , Espectrometría de Masas en Tándem/métodos , Animales , Benzaldehídos/sangre , Benzaldehídos/química , Catecoles/sangre , Catecoles/química , Cinamatos/sangre , Cinamatos/química , Depsidos/sangre , Depsidos/química , Medicamentos Herbarios Chinos/farmacocinética , Flavanonas/sangre , Flavanonas/química , Glucósidos/sangre , Glucósidos/química , Modelos Lineales , Masculino , Ratas , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Ácido Rosmarínico
6.
Biomed Chromatogr ; 32(2)2018 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-28744886

RESUMEN

A sensitive, specific and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of seven constituents of the Zaoren Anshen prescription (ZAP) in rat plasma after oral administration of the ZAP: spinosin, salvianic acid A, 6'''-feruloylspinosin, protocatechualdehyde, salvianolic acid B, schisandrin and deoxyschisandrin. The plasma samples and the internal standard (IS) sulfamethoxazole were extracted using acetonitrile. Chromatographic separation was performed with an Agilent HC-C18 column using a gradient elution profile and a mobile phase consisting of 0.01% formic acid in water (A) and acetonitrile (B). The analytes were quantified simultaneously in a single run using an ion trap mass spectrometer operated in the multiple reaction monitoring mode and electrospray ion-source polarity in the positive and negative modes. The calibration curves for spinosin, salvianic acid A, 6'''-feruloylspinosin, protocatechualdehyde, salvianolic acid B, schisandrin and deoxyschisandrin were linear over the concentration ranges of 2.90-1160, 2.50-1000, 1.80-720, 0.65-260, 2.50-1000, 8.00-1600 and 1.30-520 ng/mL, respectively. The intra- and inter-day precisions in terms of relative standard deviation were <18.9%, and the accuracies in terms of relative error were within ±14.2%. Consequently, the proposed method was successfully applied to the pharmacokinetic analysis of these seven major active compounds in rats administered ZAP. These results will facilitate research aiming to predict the effectiveness of the optimal dose of ZAP and might be beneficial for the therapeutic use of ZAP in the clinical setting.


Asunto(s)
Benzaldehídos/sangre , Benzofuranos/sangre , Catecoles/sangre , Ciclooctanos/sangre , Medicamentos Herbarios Chinos/farmacocinética , Flavonoides/sangre , Lignanos/sangre , Compuestos Policíclicos/sangre , Animales , Benzaldehídos/química , Benzaldehídos/farmacocinética , Benzofuranos/química , Benzofuranos/farmacocinética , Catecoles/química , Catecoles/farmacocinética , Cromatografía Liquida/métodos , Ciclooctanos/química , Ciclooctanos/farmacocinética , Estabilidad de Medicamentos , Medicamentos Herbarios Chinos/análisis , Medicamentos Herbarios Chinos/química , Flavonoides/química , Flavonoides/farmacocinética , Lignanos/química , Lignanos/farmacocinética , Límite de Detección , Modelos Lineales , Masculino , Compuestos Policíclicos/química , Compuestos Policíclicos/farmacocinética , Ratas , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Espectrometría de Masas en Tándem/métodos
7.
Biomed Chromatogr ; 31(8)2017 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-28146302

RESUMEN

In this study, a rapid and reliable ultra-fast liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of eight active ingredients, including astragaloside IV, ononin, tanshinol, protocatechualdehyde, protocatechuic acid, salvianolic acid D, rosmarinic acid and ginsenoside Rg1 , in rat plasma. The plasma samples were pretreated by protein precipitation with acetonitrile. Chromatographic separation was performed on a Waters Acquity UPLC® BEH C18 column (1.7 µm particles, 2.1 × 100 mm). The mobile phase consisted of 0.1% aqueous formic acid (A)-acetonitrile with 0.1% formic acid (B) at a flow rate of 0.4 mL/min. Quantification was performed on a triple quadruple tandem mass spectrometry with electrospray ionization by multiple reaction monitoring both in the negative and in the positive ion mode. The lower limit of quantification of tanshinol was 2.0 ng/mL and the others were 5.0 ng/mL. The extraction recoveries, matrix effects, intra- and inter-day precision and accuracy of eight tested components were all within acceptable limits. The validated method was successfully applied to the pharmacokinetic study of the eight active constituents after intragastric administration of three doses (1.0, 3.0, 6.0 g/kg body weight) of Qishen Yiqi Dripping Pills to rats.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacocinética , Espectrometría de Masas en Tándem/métodos , Alquenos/análisis , Alquenos/sangre , Animales , Benzaldehídos/análisis , Benzaldehídos/sangre , Ácidos Cafeicos/análisis , Ácidos Cafeicos/sangre , Catecoles/análisis , Catecoles/sangre , Cinamatos/análisis , Cinamatos/sangre , Depsidos/análisis , Depsidos/sangre , Ginsenósidos/análisis , Ginsenósidos/sangre , Glucósidos/análisis , Glucósidos/sangre , Hidroxibenzoatos/análisis , Hidroxibenzoatos/sangre , Isoflavonas/análisis , Isoflavonas/sangre , Límite de Detección , Masculino , Polifenoles/análisis , Polifenoles/sangre , Ratas , Ratas Sprague-Dawley , Saponinas/análisis , Saponinas/sangre , Triterpenos/análisis , Triterpenos/sangre , Ácido Rosmarínico
8.
Biomed Chromatogr ; 31(5)2017 May.
Artículo en Inglés | MEDLINE | ID: mdl-27808441

RESUMEN

A sensitive and reliable ultra-high performance liquid chromatography coupled with tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed for quantitation of plantamajoside in rat plasma. First, this study compared the pharmacokinetic properties of plantamajoside after oral administration of Plantago asiatica extract and pure plantamajoside in rat plasma with approximately the same dosage of 8.98 mg/kg. Second, chromatographic separation was performed on an Acquity HSS C18 column (50 × 2.1 mm, p.d.1.7 µm) with isocratic elution using methanol-water (80:20, v/v) as mobile phase at a flow rate of 0.25 mL/min. The calibration curves were linear over the range of 0.1-100 ng/mL for plantamajoside. At different time points (0, 0.083, 0.167, 0.25, 0.5, 0.75, 1, 2, 3, 4, 5, 6 and 8 h) after administration, the concentrations of plantamajoside in plasma were measured and the main pharmacokinetic parameters were estimated. The study indicates that the pharmacokinetics of plantamajoside in rat plasma have significant differences between two groups.


Asunto(s)
Catecoles/sangre , Catecoles/farmacocinética , Glucósidos/sangre , Glucósidos/farmacocinética , Extractos Vegetales/farmacocinética , Plantago/química , Administración Oral , Animales , Catecoles/administración & dosificación , Cromatografía Líquida de Alta Presión/métodos , Estabilidad de Medicamentos , Glucósidos/administración & dosificación , Límite de Detección , Masculino , Extractos Vegetales/administración & dosificación , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Extracción en Fase Sólida , Espectrometría de Masas en Tándem/métodos
9.
Artículo en Inglés | MEDLINE | ID: mdl-27700987

RESUMEN

The bio-active compounds of ginger (Zingiber officinale Roscoe), the gingerols, are gaining considerable attention due to their numerous beneficial health effects. In order to elucidate the physiological relevance of the ascribed effects their bioavailability has to be determined taking their metabolization into account. To quantitate in vivo generated [6]-, [8]- and [10]-gingerol glucuronides in human plasma and urine after ginger tea consumption, a simultaneous and direct liquid chromatography-tandem mass spectrometry method based on stable isotope dilution assays was established and validated. The respective references as well as the isotopically labeled substances were synthesized and characterized by mass spectrometry and NMR. Selective isolation of gingerol glucuronides from human plasma and urine by a mixed-phase anion-exchange SPE method led to recovery rates between 80.8 and 98.2%. LC-MS/MS analyses in selected reaction monitoring modus enabled a highly sensitive quantitation of gingerol glucuronides with LoQs between 3.9-9.8nmol/L in plasma and 39.3-161.1nmol/L in urine. The method precision in plasma and urine varied in the range±15%, whereas the intra-day accuracy in plasma and urine showed values between 78 and 122%. The developed method was then applied to a pilot study in which two volunteers consumed one liter ginger tea. Pharmacokinetic parameters like the maximum concentration (cmax), the time to reach cmax (tmax), area under the curve (AUC), elimination rate constant (kel) and elimination half-life (t1/2) were calculated from the concentration-time curve of each gingerol glucuronide. The obtained results will enable more detailed investigation of gingerol glucuronides as bioactives in their physiologically relevant concentrations.


Asunto(s)
Catecoles/sangre , Catecoles/orina , Cromatografía Líquida de Alta Presión/métodos , Alcoholes Grasos/sangre , Alcoholes Grasos/orina , Glucurónidos/sangre , Glucurónidos/orina , Espectrometría de Masas en Tándem/métodos , Catecoles/análisis , Alcoholes Grasos/análisis , Femenino , Zingiber officinale/química , Glucurónidos/análisis , Humanos , Técnicas de Dilución del Indicador , Límite de Detección , Proyectos Piloto , Té/química
10.
Eur J Pharm Sci ; 93: 341-50, 2016 Oct 10.
Artículo en Inglés | MEDLINE | ID: mdl-27568854

RESUMEN

The present study was aimed to develope a proliposomal formulation to decrease the hepatic first-pass metabolism of protocatechualdehyde (PD), followed by pellet coating to modify the drug release for angina chronotherapy. PD proliposomes were prepared by depositing PD-phospholipid complex on mannitol powders to improve the drug encapsulation. Afterwards, the PD proliposomes were prepared into pellet cores via extrusion-spheronization using 10% κ-carrageenan as pelletization aid prior to the development of PD sustained-release pellets (PD-SRPs). Eudragit® NE 30D was chosen as coating material and the desired drug release profile of PD-SRPs was calculated for formulation optimization by deconvolution based on the circadian rhythm of variant angina. A high similarity factor (f2=85.72) was achieved when the coating weight was 30% and the sustained release behavior also prevented the destruction of liposomes by gastric fluids. Pharmacokinetic studies revealed a basically consistent trend between the actual and the predicted plasma concentration-time curve with absolute percent errors (%PE) of concentrations <10% in 2-12h. Meanwhile, a relative bioavailability of 200% was achieved compared with pure PD. Therefore, the development of proliposomes-based PD-SRPs was an effective strategy to provide both improved oral bioavailability and desired drug plasma concentration-time course for angina chronotherapy.


Asunto(s)
Angina de Pecho/metabolismo , Anticoagulantes/administración & dosificación , Anticoagulantes/farmacocinética , Benzaldehídos/administración & dosificación , Benzaldehídos/farmacocinética , Catecoles/administración & dosificación , Catecoles/farmacocinética , Cronoterapia , Angina de Pecho/sangre , Animales , Anticoagulantes/sangre , Benzaldehídos/sangre , Disponibilidad Biológica , Catecoles/sangre , Preparaciones de Acción Retardada/administración & dosificación , Preparaciones de Acción Retardada/farmacocinética , Composición de Medicamentos , Liberación de Fármacos , Liposomas , Masculino , Manitol/química , Fosfolípidos/química , Conejos
11.
Artículo en Inglés | MEDLINE | ID: mdl-26118621

RESUMEN

A rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous determination of the four major active ingredients, danshensu, protocatechuic aldehyde, rosmarinic acid, and ligustrazine, in the traditional Chinese medicine Shenxiong glucose injection in rat plasma. Acidified and alkalized plasma samples were extracted using ethyl acetate, and separated on a Waters C18 column (2.1mm×50mm, 1.7µm) by using a gradient mobile phase system of acetonitrile-water containing 0.1% formic acid and luteoloside as an internal standard. Electrospray ionization in the positive-ion mode and multiple reaction monitoring were used to identify and quantitate the active components. All calibration curves showed good linearity (r>0.994) over the concentration range, with a lower limit of quantification (LLOQ) between 0.02 and 0.21µg/mL. The precision of the in vivo study was evaluated by intra- and inter-day assays, and the percentage of relative standard deviation was within 15%. Moreover, satisfactory extraction efficiency was obtained (between 83.94 and 117.81%) by liquid-liquid extraction. The validated method was successfully applied in a pharmacokinetic study in rats after intravenous administration of Shenxiong glucose injection. The results showed that the four bioactive ingredients in Shenxiong glucose injection have linear pharmacokinetic properties in rats after intravenous injection within the administered dose range and partially different ones compared to single ingredient.


Asunto(s)
Benzaldehídos/sangre , Catecoles/sangre , Cromatografía Líquida de Alta Presión/métodos , Cinamatos/sangre , Depsidos/sangre , Lactatos/sangre , Pirazinas/sangre , Animales , Benzaldehídos/química , Benzaldehídos/farmacocinética , Catecoles/química , Catecoles/farmacocinética , Cinamatos/química , Cinamatos/farmacocinética , Depsidos/química , Depsidos/farmacocinética , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/farmacocinética , Femenino , Inyecciones Intravenosas , Lactatos/química , Lactatos/farmacocinética , Límite de Detección , Modelos Lineales , Masculino , Pirazinas/química , Pirazinas/farmacocinética , Ratas Sprague-Dawley , Reproducibilidad de los Resultados , Espectrometría de Masas en Tándem/métodos , Ácido Rosmarínico
12.
Zhongguo Zhong Yao Za Zhi ; 39(9): 1704-8, 2014 May.
Artículo en Chino | MEDLINE | ID: mdl-25095388

RESUMEN

To develop a LC-MS/MS method for the determination of protocatechuic acid, protocatechuic aldehyde, salvianolic acid A, salvianolic acid B, cryptotanshinone and tanshinone II(A) in rat plasma and brain. The plasma and brain samples were precipitated with ethyl acetate, then were separated on an Agilent eclipse plus-C18 column (2.1 mm x 50 mm, 3.5 microm) using acetonitrile (consisting of 0.1% formic acid) and water (consisting of 0.1% formic acid) as mobile phase in gradient elution mode. The mass spectrometer was operated under both positive and negative ion mode with the ESI source, and the detection was performed by MRM. The transition of 154.3/153.1 m/z for protocatechuic acid, 137.3/108 m/z for protocatechuic aldehyde, 493.0/295.2 m/z for Salvianolic acid A, 718.0/520.0 m/z for salvianolic acid B, 321.4/152.3 m/z for chloramphenicol, 297.4/254.3 m/z for cryptotanshinone, 295.5/249.3 m/z for tanshinone II(A) and 285.2/154.0 m/z for Diazepam. The calibration curves in the range of 0.625-1 000 microg x L(-1) for protocatechuic acid and protocatechuic aldehyde, 1.25-1 000 microg x L(-1) for salvianolic acid A, 2.5-1 000 microg x L(-1) for salvianolic acid B, 0.15-1 000 microg x L(-1) for cryptotanshinone, 0.625-1 000 microg x L(-1) for tanshinone II(A) are with good linearityin rat plasma and brain. The analysis method is sensitive, simple, and suitable enough to be applied in the pharmacokinetic study of the 6 main components. Animal testing gives the lgBB of the drugs and further studies of the 6 components cross the blood-brain barrier can be carried out.


Asunto(s)
Encéfalo/metabolismo , Cromatografía Liquida/métodos , Preparaciones de Plantas/sangre , Preparaciones de Plantas/farmacocinética , Salvia miltiorrhiza/química , Espectrometría de Masas en Tándem/métodos , Abietanos/administración & dosificación , Abietanos/sangre , Abietanos/farmacocinética , Animales , Benzaldehídos/administración & dosificación , Benzaldehídos/sangre , Benzaldehídos/farmacocinética , Benzofuranos/administración & dosificación , Benzofuranos/sangre , Benzofuranos/farmacocinética , Barrera Hematoencefálica/metabolismo , Ácidos Cafeicos/administración & dosificación , Ácidos Cafeicos/sangre , Ácidos Cafeicos/farmacocinética , Catecoles/administración & dosificación , Catecoles/sangre , Catecoles/farmacocinética , Hidroxibenzoatos/administración & dosificación , Hidroxibenzoatos/sangre , Hidroxibenzoatos/farmacocinética , Inyecciones Intravenosas , Lactatos/administración & dosificación , Lactatos/sangre , Lactatos/farmacocinética , Fenantrenos/administración & dosificación , Fenantrenos/sangre , Fenantrenos/farmacocinética , Preparaciones de Plantas/administración & dosificación , Ratas , Reproducibilidad de los Resultados
13.
Planta Med ; 79(16): 1489-94, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-24146062

RESUMEN

After oral administration of 100 mg/kg b. w. (235.8 µmol/kg) salicortin to Wistar rats, peak serum concentrations of 1.43 mg/L (13.0 µM) catechol were detected after 0.5 h in addition to salicylic acid by HPLC-DAD after serum processing with ß-glucuronidase and sulphatase. Both metabolites could also be detected in the serum of healthy volunteers following oral administration of a willow bark extract (Salicis cortex, Salix spec., Salicaceae) corresponding to 240 mg of salicin after processing with both enzymes. In humans, the cmax (1.46 mg/L, 13.3 µM) of catechol was reached after 1.2 h. The predominant phase-II metabolite in humans and rats was catechol sulphate, determined by HPLC analysis of serum samples processed with only one kind of enzyme. Without serum processing with glucuronidase and sulphatase, no unconjugated catechol could be detected in human and animal serum samples. As catechol is described as an anti-inflammatory compound, these results may contribute to the elucidation of the mechanism of the action of willow bark extract.


Asunto(s)
Catecoles/sangre , Glucósidos/farmacocinética , Salix/química , Administración Oral , Animales , Catecoles/química , Cromatografía Líquida de Alta Presión , Glucósidos/administración & dosificación , Glucósidos/química , Humanos , Ratas , Ratas Wistar
14.
Eur J Mass Spectrom (Chichester) ; 17(4): 395-403, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-22006631

RESUMEN

Salvianolic acid A, salvianolic acid B, danshensu, protocatechuic aldehyde, rosmarinic acid and lithospermic acid are the six major active constituents in Danshen injection. In this study, a rapid, sensitive and specific liquid chromatographic-electrospray ionization-mass spectrometry method for the simultaneous quantitative determination of these compounds in rat plasma was developed. After a single step of liquid-liquid extraction with ethyl acetate, they were eluted by a Hypersil C18 column (5 µm, i.d. 4.6 × 200 mm) within 4 min with a mobile phase consisting of acetonitrile and 0.1% formic acid water solution (35:65, v/v). The assay was linear in the concentration range of 0.05-10 µg mL(-1). Absolute recoveries were above 60%. The precisions and accuracies determined within three consecutive days were within acceptable limits. The method was successfully applied to a pharmacokinetic study in rats after an intravenous administration of Danshen injection.


Asunto(s)
Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/farmacocinética , Extracción Líquido-Líquido/métodos , Espectrometría de Masas/métodos , Preparaciones de Plantas/administración & dosificación , Salvia miltiorrhiza/química , Espectrometría de Masa por Ionización de Electrospray/métodos , Animales , Benzaldehídos/sangre , Benzaldehídos/farmacocinética , Benzofuranos/sangre , Benzofuranos/farmacocinética , Ácidos Cafeicos/sangre , Ácidos Cafeicos/farmacocinética , Catecoles/sangre , Catecoles/farmacocinética , Cromatografía Líquida de Alta Presión/métodos , Cromatografía Liquida/métodos , Cinamatos/sangre , Cinamatos/farmacocinética , Depsidos/sangre , Depsidos/farmacocinética , Estabilidad de Medicamentos , Medicamentos Herbarios Chinos/química , Inyecciones Intravenosas , Lactatos/sangre , Lactatos/farmacocinética , Masculino , Preparaciones de Plantas/sangre , Preparaciones de Plantas/química , Ratas , Estándares de Referencia , Sensibilidad y Especificidad , Ácido Rosmarínico
15.
Biomed Chromatogr ; 25(8): 913-24, 2011 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-21058413

RESUMEN

A high-performance liquid chromatography with diode-array detection coupled to time-of-flight mass spectrometry (HPLC/DAD/TOFMS) method was established to clarify the chemical composition of Sini decoction (SND) and rat plasma after oral administration of SND. With dynamic adjustment of fragmentor voltage in TOFMS, an efficient transmission of the ions was achieved to obtain the best sensitivity for providing the molecular formula for each analyte and abundant fragment ions for structural information. By accurate mass measurements within 5 ppm error for each molecular ion and subsequent fragment ions, 53 compounds including diterpenoid alkaloids, flavonoids, triterpenoids and gingerol-related compounds were identified in SND. Major compounds identified from SND were further assigned in the three individual herbs. After oral administration of SND, 33 compounds and five metabolites in rat plasma were detected and identified by comparing and contrasting the compounds measured in SND with those in the plasma samples by HPLC/DAD/TOFMS. The results provided helpful chemical information for further pharmacology and active mechanism research on SND.


Asunto(s)
Alcaloides/sangre , Diterpenos/sangre , Medicamentos Herbarios Chinos/química , Flavonoides/sangre , Saponinas/sangre , Alcaloides/química , Animales , Catecoles/sangre , Catecoles/química , Cromatografía Líquida de Alta Presión/métodos , Diterpenos/química , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/farmacocinética , Alcoholes Grasos/sangre , Alcoholes Grasos/química , Flavonoides/química , Masculino , Ratas , Ratas Sprague-Dawley , Saponinas/química , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos
16.
J Nat Med ; 64(3): 281-7, 2010 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-20238179

RESUMEN

To investigate the pharmacokinetics of [6]-shogaol, a pungent ingredient of Zingiber officinale Roscoe, the pharmacokinetic parameters were determined by using (14)C-[6]-shogaol (labeled compound) and [6]-shogaol (non-labeled compound). When the labeled compound was orally administered to rats, the maximum plasma concentration (C (max)) and the area under the curve (AUC) of plasma radioactivity concentration increased in a dose-dependent manner. When the labeled compound was orally administered at a dose of 10 mg/kg, 20.0 + or - 1.8% of the radioactivity administered was excreted into urine, 64.0 + or - 12.9% into feces, and 0.2 + or - 0.1% into breath. Thus, more of the radioactivity was excreted into feces than into urine, and almost no radioactivity was excreted into breath. Furthermore, when the labeled compound was orally administered at a dose of 10 mg/kg, cumulative biliary radioactivity excretion over 48 h was 78.5 + or - 4.5% of the radioactivity administered, and cumulative urinary radioactivity excretion over 48 h was 11.8 + or - 2.7%, showing that about 90% of the dose administered orally was absorbed from the digestive tract and most of the fecal excretion was via biliary excretion. On the other hand, when the non-labeled compound [6]-shogaol was orally administered, the plasma concentration and biliary excretion of the unchanged form were extremely low. When these results are combined with those obtained with the labeled compound, it would suggest that [6]-shogaol is mostly metabolized in the body and excreted as metabolites.


Asunto(s)
Catecoles/farmacocinética , Zingiber officinale/química , Animales , Radioisótopos de Carbono/metabolismo , Catecoles/sangre , Catecoles/metabolismo , Catecoles/orina , Heces/química , Masculino , Ratas , Ratas Sprague-Dawley
17.
Yao Xue Xue Bao ; 42(3): 301-7, 2007 Mar.
Artículo en Chino | MEDLINE | ID: mdl-17520831

RESUMEN

The pharmacokinetics of the main components of protocatechualdehyde, salvianolic acid B, tanshinone II(A), cryptotanshinone, and the hydrophilic or lipophilic extracts of Salvia Miltiorrhiza Bge., in rat plasma were studied after oral administration separately to explore the interactions between them. Some components in the hydrophilic extract depress the absorption of the protocatechualdehyde, on the contrary, enhance the absorption of the salvianolic acid B and depress its elimination rate. The concomitant components in the lipophilic extract might enhance the absorption of cryptotanshinone and its distribution from the centre compartment to the peripheral compartment, and the metabolism to tanshinone II(A). The 'concomitant components' in the extract of Chinese material medica had significant effect on the pharmacokinetics of its 'marker components'. It can not only be rival, synergic, but also have the effects on metabolism. Therefore the traditional Chinese medicine was a complicated system, It should be taken a scientific and dialectic view in the research and development processes.


Asunto(s)
Benzaldehídos/farmacocinética , Benzofuranos/farmacocinética , Catecoles/farmacocinética , Fenantrenos/farmacocinética , Salvia miltiorrhiza/química , Abietanos , Animales , Área Bajo la Curva , Benzaldehídos/sangre , Benzaldehídos/química , Benzofuranos/sangre , Benzofuranos/química , Catecoles/sangre , Catecoles/química , Interacciones Farmacológicas , Femenino , Masculino , Tasa de Depuración Metabólica , Estructura Molecular , Fenantrenos/sangre , Fenantrenos/química , Extractos Vegetales/sangre , Extractos Vegetales/química , Extractos Vegetales/farmacocinética , Plantas Medicinales/química , Ratas , Ratas Sprague-Dawley
18.
Artículo en Inglés | MEDLINE | ID: mdl-15866491

RESUMEN

The six phenolic constituents are water-soluble components extracted from the Chinese medical herb danshen, the dried roots of Salvia miltiorrhiza Bunge (Labiatae). An liquid chromatography/tandem mass spectrometry (LC/MS/MS)-based method has been developed for the simultaneous quantification of six phenolic constituents of danshen (magnesium lithospermate B (MLB), rosmarinic acid (RA) and lithospermic acid (LA), caffeic acid (CAA), protocatechuic aldehyde (3,4-dihydroxybenzaldehyde, Pal), 3,4-dihydroxyphenyllactic acid (danshensu)) in human serum with chloramphenicol as internal standard. The serum samples were treated by special liquid-liquid extraction, and the analytes were determined using electrospray negative ionization mass spectrometry in the multiple reaction monitoring (MRM) mode, with sufficient sensitivity to allow analysis of human serum samples generated following administration of a clinically relevant dose. Good linearity over the range 8-2048 ng/mL for six phenolic constituents was observed. The intra- and inter-day precisions (CV) of analysis were <13%, and the accuracy ranged from 88 to 116%. This quantitation method was successfully applied to a pharmacokinetic study of i.v. drip infusion of Danshen injection fluid in human.


Asunto(s)
Fenoles/sangre , Salvia miltiorrhiza/química , Espectrometría de Masa por Ionización de Electrospray/métodos , Benzaldehídos/sangre , Benzofuranos/sangre , Ácidos Cafeicos/sangre , Catecoles/sangre , Cromatografía Liquida/métodos , Cinamatos/sangre , Depsidos , Estabilidad de Medicamentos , Medicamentos Herbarios Chinos/análisis , Humanos , Lactatos/sangre , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Ácido Rosmarínico
19.
J Chromatogr Sci ; 41(6): 327-30, 2003 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-12935306

RESUMEN

A simple and sensitive high-performance liquid chromatography method is developed for the simultaneous determination of (3,4-dihydroxyphenyl)-lactic acid (Dhpl) and protocatechuic aldehyde (Pal) in rat serum after oral administration of Radix Salviae miltiorrhizae extract. Serum samples are acidified with hydrochloric acid and extracted with ethyl acetate. Analysis of the extract is performed on a reversed-phase column and a mobile phase of 0.02% phosphoric acid-acetonitrile (91:9, v/v) with UV detection at 280 nm. Standard curves are linear in the range of 1.47-456.96 microg/mL for Dhpl and 0.124-7.936 microg/mL for Pal. For both regression coefficients, r(2) is greater than 0.993. Mean recovery is determined to be 75.23% and 84.06%, respectively, by analyzing serum standard containing 7.14, 57.12, and 228.48 microg/mL of Dhpl and 0.124, 0.992, and 3.968 microg/mL of Pal. The intraday precision (relative standard deviation) ranges from 3.91% to 12.03% at concentrations of 1.43, 57.12, and 228.48 microg/mL for Dhpl and 3.79% to 8.12% at concentrations of 0.124, 0.992, and 3.968 microg/mL for Pal. The interday precision (relative standard deviation) ranges from 5.06% to 9.93% for Dhpl and 3.05% to 10.00% for Pal, respectively, at the same three concentrations. This validated assay is applied to the determination of Dhpl and Pal concentrations and used to take a limited view of the pharmacokinetic profile in rat serum after oral administration of Radix Salviae miltiorrhizae extract.


Asunto(s)
Benzaldehídos/farmacocinética , Catecoles/farmacocinética , Medicamentos Herbarios Chinos/química , Lactatos/farmacocinética , Extractos Vegetales/administración & dosificación , Administración Oral , Animales , Benzaldehídos/administración & dosificación , Benzaldehídos/sangre , Calibración , Catecoles/administración & dosificación , Catecoles/sangre , Lactatos/administración & dosificación , Lactatos/sangre , Ratas , Reproducibilidad de los Resultados , Salvia miltiorrhiza , Sensibilidad y Especificidad
20.
J Chromatogr B Biomed Appl ; 662(1): 71-8, 1994 Dec 02.
Artículo en Inglés | MEDLINE | ID: mdl-7894695

RESUMEN

Methanol extracts of the corms of Hypoxis rooperi and H. latifolia were studied for their hypoxoside content by an in-line sorption enrichment HPLC technique [Kruger et al., J. Chromatogr., 612 (1993) 191]. Hypoxoside is the trivial name for (E)-1,5-bis(3'-hydroxy-4'-O-beta-D-glucopyranosyl-phenyl) pent-1-en-4-yne and rooperol the aglucone obtained from beta-glucosidase treatment. Hypoxoside and rooperol analogues containing 4, 3 and 2 hydroxyl groups resolved as separate peaks with the proportion of the latter two markedly higher in H. latifolia than in H. rooperi. After oral ingestion of hypoxoside by humans, no hypoxoside or rooperol appeared in the serum. Only rooperol was present in the faeces. The serum and urine contained at least three phase II metabolite peaks. Selective enzyme hydrolysis showed that they represent the diglucuronide, disulfate and glucuronide-sulfate conjugates of all three rooperol analogues.


Asunto(s)
Alquinos/análisis , Antineoplásicos/análisis , Catecoles/análisis , Glucósidos/análisis , Inhibidores de la Lipooxigenasa/análisis , Plantas Medicinales/química , Alquinos/sangre , Alquinos/orina , Antineoplásicos/sangre , Antineoplásicos/orina , Biotransformación , Catecoles/sangre , Catecoles/orina , Cromatografía Líquida de Alta Presión , Glucósidos/sangre , Glucósidos/orina , Humanos , Hidrólisis , Inhibidores de la Lipooxigenasa/sangre , Inhibidores de la Lipooxigenasa/orina , Raíces de Plantas/química
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