RESUMEN
Several proteins have their normal patterns of distributions altered by monocular visual deprivation. We studied the distribution of the calcium-binding proteins calbindin-28kD (Cb) and parvalbumin (Pv) in V1 in normal adult Cebus apella monkeys and in monkeys with monocular retinal lesions. In normal monkeys, the interblobs regions in layers 2/3 and the layer 4B are intensely labeled for Cb, while Pv reaction showed a complementary labeling pattern with a stronger staining in layers 4A, 4C and in the blob regions in layers 2/3. In monkeys with monocular retinal lesion, the laminar distribution of these proteins was differentially affected, although both reactions resulted in stronger labeling in non-deprived ocular dominance columns. While Cb reaction resulted in stronger labeling in layers 1 through 5, Pv labeling was heavier in layers 2/3, 4A and 4C. There was a clear reduction in the intensity of neuropil staining for both Pv and Cb in deprived ocular dominance columns with little or no reduction in number of labeled cells. This reduction could thus be attributed to activity-dependent changes at synapses level.
Asunto(s)
Cebus/fisiología , Parvalbúminas/metabolismo , Enfermedades de la Retina/metabolismo , Proteína G de Unión al Calcio S100/metabolismo , Trastornos de la Visión/metabolismo , Corteza Visual/metabolismo , Vías Visuales/metabolismo , Animales , Calbindinas , Cebus/anatomía & histología , Modelos Animales de Enfermedad , Predominio Ocular/fisiología , Complejo IV de Transporte de Electrones/metabolismo , Inmunohistoquímica , Neuronas/citología , Neuronas/metabolismo , Neurópilo/metabolismo , Neurópilo/ultraestructura , Filogenia , Enfermedades de la Retina/fisiopatología , Especificidad de la Especie , Sinapsis/metabolismo , Sinapsis/ultraestructura , Trastornos de la Visión/fisiopatología , Corteza Visual/citología , Vías Visuales/fisiopatologíaRESUMEN
We have used retrograde transneuronal transport of neurotropic viruses to examine the organization of the projections from the dentate nucleus of the cerebellum to "motor" and "nonmotor" areas of the cerebral cortex. To perform this analysis we created an unfolded map of the dentate. Plotting the results from current and prior experiments on this unfolded map revealed important features about the topography of function in the dentate. We found that the projections to the primary motor and premotor areas of the cerebral cortex originated from dorsal portions of the dentate. In contrast, projections to prefrontal and posterior parietal areas of cortex originated from ventral portions of the dentate. Thus the dentate contains anatomically separate and functionally distinct motor and nonmotor domains.