RESUMEN
Arabinoxylans are part of dietary fibre and have received attention given their emergent prebiotic character. Four arabinoxylans extracts were obtained from Argentinian soft and hard wheat. In vitro assays were performed to describe the extent to which the extracts from whole wheat flour support selective growth of Bifidobacterium breve and probiotic Lactobacillus reuteri ATCC23272 in a defined media. The prebiotic effect was evaluated by three quantitative scores: relative growth, prebiotic activity score and prebiotic index. For prebiotic index equation the growth of Bacteroides and Clostridium strains was compared to that of bifidobacteria and lactic acid bacteria. All the arabinoxylans extracts supported the growth of Lactobacillus and Bifidobacterium, reaching higher prebiotic activity score values than inulin (0·37 and 0·36 for Lactobacillus and Bifidobacterium respectively). AX2 from soft wheat and AX4 from hard showed similar prebiotic index value to commercial inulin (2·64, 2·52 and 2·22 respectively), and AX3 extract presented higher prebiotic index value (4·09) than the positive control and other prebiotic index reported for arabinoxylans. These extracts could be used as prebiotic, synbiotic compositions or novel food prototypes to treat dysbiosis associated with many diseases. SIGNIFICANCE AND IMPACT OF THE STUDY: The present work demonstrates that AX extracts from Argentinian soft and hard wheat promote efficiently the growth of probiotic strain L. reuteri ATCC23272 and B. breve 286, validated with three different parameters that consider the growth of representative strains of Bacteria genera found in the gut. The evaluation of AX extracts as a food supplement in a murine model could confirm their ability to modulate the microbiome. Novel food prototypes including AX and probiotics could relieve local symptoms and may act as psychobiotics with a beneficial effect on microbiome-brain axis.
Asunto(s)
Bifidobacterium breve/crecimiento & desarrollo , Limosilactobacillus reuteri/crecimiento & desarrollo , Preparaciones de Plantas/farmacología , Triticum/química , Xilanos/farmacología , Bacteroides/crecimiento & desarrollo , Clostridium/crecimiento & desarrollo , Fibras de la Dieta , Prebióticos/microbiología , Probióticos/metabolismo , SimbióticosRESUMEN
Synthesis gas (syngas) fermentation via the Wood-Ljungdahl pathway is receiving growing attention as a possible platform for the fixation of CO2 and renewable production of fuels and chemicals. However, the pathway operates near the thermodynamic limit of life, resulting in minimal adenosine triphosphate (ATP) production and long doubling times. This calls into question the feasibility of producing high-energy compounds at industrially relevant levels. In this study, we investigated the possibility of co-utilizing nitrate as an inexpensive additional electron acceptor to enhance ATP production during H2 -dependent growth of Clostridium ljungdahlii, Moorella thermoacetica, and Acetobacterium woodii. In contrast to other acetogens tested, growth rate and final biomass titer were improved for C. ljungdahlii growing on a mixture of H2 and CO2 when supplemented with nitrate. Transcriptomic analysis, 13CO2 labeling, and an electron balance were used to understand how electron flux was partitioned between CO2 and nitrate. We further show that, with nitrate supplementation, the ATP/adenosine diphosphate (ADP) ratio and acetyl-CoA pools were increased by fivefold and threefold, respectively, suggesting that this strategy could be useful for the production of ATP-intensive heterologous products from acetyl-CoA. Finally, we propose a pathway for enhanced ATP production from nitrate and use this as a basis to calculate theoretical yields for a variety of products. This study demonstrates a viable strategy for the decoupling of ATP production from carbon dioxide fixation, which will serve to significantly improve the CO2 fixation rate and the production metrics of other chemicals from CO2 and H2 in this host.
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Acetobacterium/metabolismo , Dióxido de Carbono/metabolismo , Clostridium/metabolismo , Hidrógeno/metabolismo , Moorella/metabolismo , Nitratos/metabolismo , Acetobacterium/crecimiento & desarrollo , Adenosina Trifosfato/biosíntesis , Ciclo del Carbono , Clostridium/crecimiento & desarrollo , Análisis de Flujos Metabólicos , Moorella/crecimiento & desarrolloRESUMEN
BACKGROUND: A laboratory-silo study was conducted to evaluate the fermentation quality, feed-nutritive value and aerobic stability of sweet sorghum silage with or without oil-extracted microalgae supplementation. Sweet sorghum was mixed with four microalgae levels (0%, 1%, 2% and 3% on a dry matter basis; control, M1, M2 and M3, respectively) and ensiled for 45 d. Further, the four experimental silages were subjected to an aerobic stability test lasting 7 d. RESULTS: All the silages except M3 silage had good fermentative characteristics with low pH and ammonia nitrogen concentrations, and high lactic acid concentrations and favorable microbial parameters. Meanwhile, oil-extracted microalgae supplementation improved the feed-nutritional value of sweet sorghum silage. Fibre (neutral detergent fibre, acid detergent fibre, acid detergent lignin and cellulose) concentrations decreased, while dry matter and crude protein levels markedly increased (P < 0.05). Compared with the control (69.7 h), treatments M2 and M3 improved the aerobic stability of sweet sorghum silage by 43.8% and more than 143% respectively, and decreased the clostridia spore counts during the stage of air exposure. CONCLUSION: Sweet sorghum silage produced with 2% oil-extracted microalgae addition was the most suitable for animal use due to the optimal balance of fermentation quality, feed-nutritional value and aerobic stability, which merits further in vivo studies using grazing ruminants. © 2018 Society of Chemical Industry.
Asunto(s)
Alimentación Animal/análisis , Microalgas/química , Extractos Vegetales/química , Rumiantes/metabolismo , Sorghum/química , Animales , Clostridium/crecimiento & desarrollo , Clostridium/metabolismo , Fermentación , Lactobacillales/crecimiento & desarrollo , Lactobacillales/metabolismo , Microalgas/metabolismo , Valor Nutritivo , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/metabolismo , Ensilaje/análisis , Sorghum/metabolismo , Levaduras/crecimiento & desarrollo , Levaduras/metabolismoRESUMEN
In vitro fermentation and in vivo feeding experiments were conducted to characterize the effects of soybean (Glycine max) husk on the fecal fermentation metabolites and microbiota of dogs. An in vitro fermentation study using feces from three Toy Poodle dogs (6.5 ± 3.5 months in age and 2.9 ± 0.4 kg in body weight) revealed that the fecal inoculum was able to ferment soybean husk (supplemented at 0.01 g/mL culture) and increased levels of short chain fatty acids (SCFA) and Bifidobacterium, irrespective of pre-digestion of the husk by pepsin and pancreatin. In a feeding experiment, four Shiba dogs (7-48 months in age and 7.5 ± 1.7 kg in body weight) fed a commercial diet supplemented with 5.6% soybean husk showed an increase in SCFA, such as acetate and butyrate, and lactate, and a decrease in indole and skatole in the feces compared to those fed a 5.6% cellulose diet. Real-time PCR assay showed that soybean husk supplementation stimulated the growth of lactobacilli, Clostridium cluster IV including Faecalibacterium prausnitzii, Clostridium cluster XIVa, Bacteroides-Prevotella-Porphyromonas group but inhibited the growth of Clostridium cluster XI. Both in vitro and in vivo experiments indicated that soybean husk supplementation improves gastrointestinal health through optimization of beneficial organic acid production and increase of beneficial bacteria. Therefore, soybean husk is suggested to be applicable as a functional fiber in the formulation of canine diets.
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Dieta/veterinaria , Perros/metabolismo , Perros/microbiología , Heces/química , Heces/microbiología , Fermentación , Microbioma Gastrointestinal , Glycine max , Animales , Bifidobacterium , Clostridium/crecimiento & desarrollo , Suplementos Dietéticos , Ácidos Grasos Volátiles/metabolismo , Técnicas In Vitro , Lactobacillus/crecimiento & desarrollo , Pancreatina , Pepsina ARESUMEN
The transformation of phosphorus added to the soil environment has been proven to be influenced by the Fe biochemical process, which thereby may affect the transformation of organic chlorinated contaminants. However, the amount of related literatures regarding this topic is limited. This study aimed to determine the effects of phosphorus addition on pentachlorophenol (PCP) anaerobic transformation, iron reduction, and paddy soil microbial community structure. Results showed that the transformation of phosphorus, iron, and PCP were closely related to the microorganisms. Moreover, phosphorus addition significantly influenced PCP transformation and iron reduction, which promoted and inhibited these processes at low and high concentrations, respectively. Both the maximum reaction rate of PCP transformation and the maximum Fe(II) amount produced were obtained at 1 mmol/L phosphorus concentration. Among the various phosphorus species, dissolved P and NaOH-P considerably changed, whereas only slight changes were observed for the remaining phosphorus species. Microbial community structure analysis demonstrated that adding low concentration of phosphorus promoted the growth of Clostridium bowmanii, Clostridium hungatei, and Clostridium intestinale and Pseudomonas veronii. By contrast, high-concentration phosphorus inhibited growth of these microorganisms, similar to the curves of PCP transformation and iron reduction. These observations indicated that Clostridium and P. veronii, especially Clostridium, played a vital role in the transformation of related substances in the system. All these findings may serve as a reference for the complicated reactions among the multiple components of soils.
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Hierro/química , Pentaclorofenol/análisis , Fósforo/química , Microbiología del Suelo , Contaminantes del Suelo/análisis , Suelo/química , China , Clostridium/crecimiento & desarrollo , Cinética , Modelos Teóricos , Oxidación-Reducción , Pentaclorofenol/química , Contaminantes del Suelo/químicaRESUMEN
We have developed butanol-producing consolidated bioprocessing from cellulosic substrates through coculture of cellulolytic clostridia and butanol-producing Clostridium saccharoperbutylacetonicum strain N1-4. However, the butanol fermentation by strain N1-4 (which has an optimal growth temperature of 30°C) is sensitive to the higher cultivation temperature of 37°C; the nature of this deleterious effect remains unclear. Comparison of the intracellular metabolites of strain N1-4 cultivated at 30°C and 37°C revealed decreased levels of multiple primary metabolites (notably including nucleic acids and cofactors) during growth at the higher temperature. Supplementation of the culture medium with 250 mg/liter adenine enhanced both cell growth (with the optical density at 600 nm increasing from 4.3 to 10.2) and butanol production (increasing from 3.9 g/liter to 9.6 g/liter) at 37°C, compared to those obtained without adenine supplementation, such that the supplemented 37°C culture exhibited growth and butanol production approaching those observed at 30°C in the absence of adenine supplementation. These improved properties were based on the maintenance of cell viability. We further showed that adenine supplementation enhanced cell viability during growth at 37°C by maintaining ATP levels and inhibiting spore formation. This work represents the first demonstration (to our knowledge) of the importance of adenine-related metabolism for clostridial butanol production, suggesting a new means of enhancing target pathways based on metabolite levels.IMPORTANCE Metabolomic analysis revealed decreased levels of multiple primary metabolites during growth at 37°C, compared to 30°C, in C. saccharoperbutylacetonicum strain N1-4. We found that adenine supplementation restored the cell growth and butanol production of strain N1-4 at 37°C. The effects of adenine supplementation reflected the maintenance of cell viability originating from the maintenance of ATP levels and the inhibition of spore formation. Thus, our metabolomic analysis identified the depleted metabolites that were required to maintain cell viability. Our strategy, which is expected to be applicable to a wide range of organisms, permits the identification of the limiting metabolic pathway, which can serve as a new target for molecular breeding. The other novel finding of this work is that adenine supplementation inhibits clostridial spore formation. The mechanism linking spore formation and metabolomic status in butanol-producing clostridia is expected to be the focus of further research.
Asunto(s)
Adenina/farmacología , Butanoles/metabolismo , Clostridium/efectos de los fármacos , Clostridium/metabolismo , Viabilidad Microbiana/efectos de los fármacos , 1-Butanol/metabolismo , Acetona/metabolismo , Adenosina Trifosfato , Clostridium/crecimiento & desarrollo , Medios de Cultivo/química , Etanol/metabolismo , Fermentación , Glucosa/metabolismo , Redes y Vías Metabólicas/efectos de los fármacos , Metabolómica , Esporas Bacterianas/efectos de los fármacos , TemperaturaRESUMEN
Clostridium carboxidivorans P7 is a strict anaerobic bacterium capable of converting syngas to biofuels. However, its fermentation profiles is poorly understood. Here, various end-products, including acetic acid, butyric acid, hexanoic acid, ethanol and butanol were supplemented to evaluate their effects on fermentation profiles in C. carboxidivorans at two temperatures. At 37°C, fatty acids addition likely led to more corresponding alcohols production. At 25°C, C2 and C4 fatty acids supplementation resulted in more corresponding higher fatty acids, while supplemented hexanoic acid increased yields of C2 and C4 fatty acids and hexanol. Supplementation of ethanol or butanol caused increased production of C2 and C4 acids at both temperatures; however, long-chain alcohols were still more likely produced at lower temperature. In conclusion, fermentation profiles of C. carboxidivorans can be changed in respond to pre-added end-products and carbon flow may be redirected to desired products by controlling culture conditions.
Asunto(s)
Biocombustibles/microbiología , Clostridium/metabolismo , Fermentación , Ácido Acético/farmacología , Biomasa , Reactores Biológicos/microbiología , Butanoles/farmacología , Caproatos/farmacología , Clostridium/efectos de los fármacos , Clostridium/crecimiento & desarrollo , Etanol/metabolismo , Ácidos Grasos/farmacología , Fermentación/efectos de los fármacos , Concentración de Iones de Hidrógeno , TemperaturaRESUMEN
BACKGROUND: Clostridium pasteurianum is becoming increasingly attractive for the production of chemicals and fuels such as n-butanol and 1,3-propanediol. Previously we have shown that dual substrate fermentation using glucose and glycerol enhanced the cell growth and butanol production significantly. Although C. pasteurianum can grow efficiently with either glucose or glycerol alone, under certain conditions, glucose limitation in the mixed substrate fermentation leads to growth cessation. To understand this phenomenon and for process optimization, fermentation experiments were performed in the presence of excess glycerol but with varied initial concentrations of glucose which were followed by physiological, metabolic and proteomic analyses. RESULTS: Physiological characterization showed that the observed cease of growth is not due to the toxicity of n-butanol. Furthermore, the growth can be resumed by addition of glucose or the intermediate oxaloacetate. Proteomic analysis shed more light on the system-level regulation of many proteins directly or indirectly associated with this phenomenon. Surprisingly, it is found that the specific growth rate of C. pasteurianum in the different growth phases (e.g. before and after glucose limitation) correlated well with the expression level of the ATP dependent pyruvate carboxylase and with the expression level of biotin synthase which provides the cofactor biotin for the formation of oxaloacetate from pyruvate. Bioenergetic analysis based on the formation rates of metabolites further show that ATP supply is not a limiting factor for the pyruvate carboxylation to oxaloacetate. CONCLUSIONS: The results of physiological and proteomic analyses clearly show that the anaplerotic synthesis of oxaloacetate plays a key role in determining the growth behaviour of C. pasteurianum in fermentations with mixed substrates of glucose and glycerol. This study provides interesting targets for metabolic engineering of this emerging industrial microorganism.
Asunto(s)
1-Butanol/metabolismo , Proteínas Bacterianas/química , Clostridium/metabolismo , Glicoles de Propileno/metabolismo , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Clostridium/química , Clostridium/genética , Clostridium/crecimiento & desarrollo , Fermentación , Glucosa/metabolismo , ProteómicaRESUMEN
AIMS: This study was performed in a well-established in vitro model to investigate whether the application of a glyphosate-containing herbicide might affect the bacterial communities and some biochemical parameters in a cow's rumen. METHODS AND RESULTS: The test item was applied in two concentrations (high and low) for 5 days. In a second trial, fermentation vessels were inoculated with Clostridium sporogenes before the high dose was applied. Effluents were analysed by biochemical, microbiological and genetic methods. A marginal increase in short-chain fatty acid production and a reduction in NH3 -N were observed. There were minor and rather equivocal changes in the composition of ruminal bacteria but no indications of a shift towards a more frequent abundance of pathogenic Clostridia species. Clostridium sporogenes counts declined consistently. CONCLUSIONS: No adverse effects of the herbicide on ruminal metabolism or composition of the bacterial communities could be detected. In particular, there was no evidence of a suspected stimulation of Clostridia growth. SIGNIFICANCE AND IMPACT OF THE STUDY: Antibiotic activity of glyphosate resulting in microbial imbalances has been postulated. In this exploratory study, however, intraruminal application of concentrations reflecting potential exposure of dairy cows or beef cattle did not exhibit significant effects on bacterial communities in a complex in vitro system. The low number of replicates (n = 3/dose) may leave some uncertainty.
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Bacterias/clasificación , Bovinos/metabolismo , Clostridium/crecimiento & desarrollo , Microbioma Gastrointestinal/efectos de los fármacos , Glicina/análogos & derivados , Herbicidas/toxicidad , Rumen/metabolismo , Animales , Bacterias/metabolismo , Bovinos/microbiología , Clostridium/clasificación , Clostridium/efectos de los fármacos , Dieta , Ácidos Grasos/análisis , Femenino , Fermentación , Jugo Gástrico/microbiología , Glicina/toxicidad , Técnicas In Vitro , Rumen/microbiología , GlifosatoRESUMEN
UNLABELLED: The need for suitable selective cultivation media for the isolation of Bifidobacterium spp. continues to be a real concern in the field of intestinal microbiology. Isolation of bifidobacteria from human and animal faecal samples using selective agar plating may be problematic especially in samples with increased clostridial counts than bifidobacterial counts. Due to the absence of anticlostridial agents in existing selective media, clostridia can displace bifidobacteria resulting in incorrect estimation of their counts. Therefore, we supplemented the existing selective medium 'modified Wilkins Chalgren agar with mupirocin' (MWM) with 90 mg l(-1) of 8-hydroxyquinoline (8HQ), which was recently proved to act selectively against clostridia. The newly composed 'modified Wilkins-Chalgren agar with 8HQ' (MWMQ) was tested on pure bifidobacterial and clostridial strains, their mixtures, and using faecal samples of mammalian origin; its selectivity was evaluated by genus-specific identification of isolates. The results demonstrated that the presence of 8HQ in this agar eliminated the growth of nonbifidobacterial strains on MWMQ compared to that on MWM, whereas the recovery of bifidobacterial counts was at satisfactory levels. In conclusion, MWMQ could be recommended for bifidobacterial isolation from human and animal faeces especially when bifidobacteria are not numerically dominant and there are chances of clostridial contamination. SIGNIFICANCE AND IMPACT OF THE STUDY: Routine isolation of bifidobacteria from mammalian faeces does not use a reliable selective agar with an anticlostridial agent. Overgrowth of clostridia may result in incorrect estimation of bifidobacterial counts. Thus, in order to improve the selectivity of existing media for bifidobacterial isolation, we chose the modified Wilkins-Chalgren agar with mupirocin and supplemented it with 8-hydroxyquinoline (8HQ), a molecule that shows anticlostridial activity without affecting the growth of bifidobacteria. This newly composed medium showed enhanced selectivity and specificity compared to the original medium and therefore, can be recommended for the isolation of bifidobacteria from mammal faeces.
Asunto(s)
Carga Bacteriana/efectos de los fármacos , Bifidobacterium/crecimiento & desarrollo , Clostridium/efectos de los fármacos , Medios de Cultivo/farmacología , Mupirocina/farmacología , Oxiquinolina/farmacología , Agar/farmacología , Animales , Bifidobacterium/aislamiento & purificación , Clostridium/crecimiento & desarrollo , Heces/microbiología , HumanosRESUMEN
While production of biofuels from renewable resources is currently receiving increased attention globally, concerns on availability and sustainability of cheap substrates for their production are growing as well. Lignocellulose-derived sugars (LDS) remain underutilized and merit consideration as a key feedstock. Among other obstacles such as low yield and low solvent titer, mitigation of stresses stemming from lignocellulose-derived microbial inhibitory compounds (LDMICs) that severely impair cell growth and solvent production is a major area of research interest. In addition to attempts at developing LDMIC-tolerant strains via metabolic engineering to enhance utilization of LDS, unconventional approaches that elicit different metabolic perturbations in microorganisms to relieve solvent- and LDMIC-mediated stresses have been explored to increase solvent production from LDS. In this review, the impacts of metabolic perturbations including medium supplementation with glycerol; furfural and 5-hydroxymethyl furfural; allopurinol, an inhibitor of xanthine dehydrogenase; calcium (Ca(2+)) and zinc (Zn(2+)) ions); and artificial electron carriers, methyl viologen and neutral red, on butanol production are discussed. Although these approaches have brought about considerable increases in butanol production, both from LDS and defined glucose-based media, the modes of action for most of these perturbations have yet to be fully characterized. Better understanding of these mechanisms would likely inform development of LDMIC-tolerant, butanol-overproducing strains, as well as possible combinatorial application of these approaches for enhanced butanol production. Hence, delineating the underlying mechanisms of these perturbations deserves further attention.
Asunto(s)
Clostridium/genética , Clostridium/metabolismo , Medios de Cultivo/metabolismo , Microbiología Industrial/métodos , Solventes/metabolismo , Butanoles/metabolismo , Clostridium/crecimiento & desarrollo , Medios de Cultivo/química , Microbiología Industrial/instrumentación , Ingeniería Metabólica , Solventes/químicaRESUMEN
This study aimed to assess the effects of a yacon-based product (YBP) on constipation in adults, including the elderly. Forty-eight individuals were recruited and divided into equal intervention groups named the test and control groups. The YBP (test) and the control (maltodextrin) were dissolved in commercial orange juice. The volunteers for the YBP/test group consumed, on a daily basis, orange juice containing 10 g fructooligosaccharide (FOS)/inulin per day. The control group consumed, on a daily basis, orange juice containing 25 g of maltodextrin. The study had a span of 30 days. We evaluated the participants' frequency of evacuation, consistency of the feces, constipation score, abdominal symptoms (flatulence, pain, and abdominal strain), and effects upon the microbiota, pH, lactate, and short-chain fatty acids (SCFAs) of the feces. The study showed an increased number of evacuations after the consumption of the YBP as well as an improvement in the consistency of the feces and a reduction in the constipation score. After 30 days of intervention, the group that consumed the YBP showed higher counts of Bifidobacterium, lower Clostridium and enterobacteria counts, and lower fecal pH. In relation to SCFAs, no significant change was found after the intervention. However, the lactate concentration was higher in the test group when compared to the post-treatment control group. The YBP was effective in improving constipation symptoms; not only was its functional characteristic in reducing constipation symptoms evident but it also demonstrated usefulness as a potential therapy.
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Asteraceae/química , Estreñimiento/dietoterapia , Defecación/efectos de los fármacos , Intestinos/efectos de los fármacos , Inulina/uso terapéutico , Oligosacáridos/uso terapéutico , Prebióticos , Adulto , Bifidobacterium/crecimiento & desarrollo , Clostridium/crecimiento & desarrollo , Estreñimiento/complicaciones , Enterobacteriaceae/crecimiento & desarrollo , Ácidos Grasos Volátiles/análisis , Heces/química , Heces/microbiología , Femenino , Humanos , Concentración de Iones de Hidrógeno , Intestinos/microbiología , Inulina/farmacología , Ácido Láctico/sangre , Masculino , Microbiota , Persona de Mediana Edad , Oligosacáridos/farmacología , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Adulto JovenRESUMEN
An anthroposophic lifestyle, which has been associated with reduced allergy risk in children, has several characteristics that could influence gut microbiota. This study aimed to investigate the impact of anthroposophic lifestyle as well as specific early life exposures on the gut microbiota. In total, 665 stool samples from 128 mother-infant pairs from the ALADDIN birth cohort study were included. Samples collected from infants at ages 6 days, 3 weeks, 2 months and 6 months, and from their mothers before and after delivery, respectively, were analyzed using 454-pyrosequencing. Information regarding lifestyle exposures was collected prospectively through interviews and questionnaires. Six-month-old infants in anthroposophic families had a significantly higher abundance of Bifidobacterium and lower abundances of Bacteroides and Veillonella. Caesarean section and breastfeeding had a significant impact on the microbiota: caesarean section was primarily associated with delayed colonization of Bifidobacterium and Bacteroides, whereas breastfed children had a higher relative abundance of Bifidobacterium and a lower abundance of Clostridiales. However, despite large differences in lifestyle exposures, we determined no significant differences in the gut microbiota between the anthroposophic and non-anthroposophic mothers or their infants' before 6 months of age.
Asunto(s)
Intestinos/microbiología , Estilo de Vida , Microbiota/fisiología , Relaciones Madre-Hijo , Adulto , Bacteroides/crecimiento & desarrollo , Bacteroides/aislamiento & purificación , Secuencia de Bases , Bifidobacterium/crecimiento & desarrollo , Bifidobacterium/aislamiento & purificación , Lactancia Materna , Cesárea , Clostridium/crecimiento & desarrollo , Estudios de Cohortes , Heces/microbiología , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Embarazo , Estudios Prospectivos , Análisis de Secuencia de ADN , Encuestas y Cuestionarios , Veillonella/crecimiento & desarrollo , Veillonella/aislamiento & purificaciónRESUMEN
We evaluated a hydrogen-based membrane biofilm reactor (MBfR) for its capacity to reduce and remove hexavalent uranium [U(VI)] from water. After a startup period that allowed slow-growing U(VI) reducers to form biofilms, the MBfR successfully achieved and maintained 94-95% U(VI) removal over 8 months when the U surface loading was 6-11 e(-) mEq/m(2)-day. The MBfR biofilm was capable of self-recovery after a disturbance due to oxygen exposure. Nanocrystalline UO2 aggregates and amorphous U precipitates were associated with vegetative cells and apparently mature spores that accumulated in the biofilm matrix. Despite inoculation with a concentrated suspension of Desulfovibrio vulgaris, this bacterium was not present in the U(VI)-reducing biofilm. Instead, the most abundant group in the biofilm community contained U(VI) reducers in the Rhodocyclaceae family when U(VI) was the only electron acceptor. When sulfate was present, the community dramatically shifted to the Clostridiaceae family, which included spores that were potentially involved in U(VI) reduction.
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Bacterias/aislamiento & purificación , Biopelículas , Uranio/aislamiento & purificación , Contaminantes Radiactivos del Agua/aislamiento & purificación , Purificación del Agua/métodos , Bacterias/crecimiento & desarrollo , Bacterias/metabolismo , Clostridium/crecimiento & desarrollo , Clostridium/aislamiento & purificación , Clostridium/metabolismo , Desulfovibrio vulgaris/crecimiento & desarrollo , Hidrógeno/química , Membranas Artificiales , ARN Ribosómico 16S/análisis , Rhodocyclaceae/crecimiento & desarrollo , Rhodocyclaceae/aislamiento & purificación , Rhodocyclaceae/metabolismo , Sulfatos/metabolismo , Uranio/metabolismoRESUMEN
The global prevalence of Fe deficiency is high and a common corrective strategy is oral Fe supplementation, which may affect the commensal gut microbiota and gastrointestinal health. The aim of the present study was to investigate the impact of different dietary Fe concentrations on the gut microbiota and gut health of rats inoculated with human faecal microbiota. Rats (8 weeks old, n 40) were divided into five (n 8 each) groups and fed diets differing only in Fe concentration during an Fe-depletion period (12 weeks) and an Fe-repletion period (4 weeks) as follows: (1) Fe-sufficient diet throughout the study period; (2) Fe-sufficient diet followed by 70 mg Fe/kg diet; (3) Fe-depleted diet throughout the study period; (4) Fe-depleted diet followed by 35 mg Fe/kg diet; (5) Fe-depleted diet followed by 70 mg Fe/kg diet. Faecal and caecal samples were analysed for gut microbiota composition (quantitative PCR and pyrosequencing) and bacterial metabolites (HPLC), and intestinal tissue samples were investigated histologically. Fe depletion did not significantly alter dominant populations of the gut microbiota and did not induce Fe-deficiency anaemia in the studied rats. Provision of the 35 mg Fe/kg diet after feeding an Fe-deficient diet significantly increased the abundance of dominant bacterial groups such as Bacteroides spp. and Clostridium cluster IV members compared with that of an Fe-deficient diet. Fe supplementation increased gut microbial butyrate concentration 6-fold compared with Fe depletion and did not affect histological colitis scores. The present results suggest that Fe supplementation enhances the concentration of beneficial gut microbiota metabolites and thus may contribute to gut health.
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Bacteroides/crecimiento & desarrollo , Clostridium/crecimiento & desarrollo , Colitis/prevención & control , Colon/microbiología , Suplementos Dietéticos , Modelos Animales de Enfermedad , Hierro de la Dieta/uso terapéutico , Animales , Bacteroides/inmunología , Bacteroides/aislamiento & purificación , Bacteroides/metabolismo , Biomarcadores/metabolismo , Ácido Butírico/metabolismo , Ciego/crecimiento & desarrollo , Ciego/inmunología , Ciego/metabolismo , Ciego/microbiología , Niño , Clostridium/inmunología , Clostridium/aislamiento & purificación , Clostridium/metabolismo , Colitis/inmunología , Colitis/metabolismo , Colitis/microbiología , Colon/crecimiento & desarrollo , Colon/inmunología , Colon/metabolismo , Suplementos Dietéticos/efectos adversos , Heces/química , Heces/microbiología , Femenino , Vida Libre de Gérmenes , Humanos , Íleon/crecimiento & desarrollo , Íleon/inmunología , Íleon/metabolismo , Íleon/microbiología , Inmunidad Mucosa , Hierro/administración & dosificación , Hierro/análisis , Deficiencias de Hierro , Hierro de la Dieta/efectos adversos , Hierro de la Dieta/análisis , Hierro de la Dieta/metabolismo , Ratas , Ratas Endogámicas F344 , Aumento de PesoRESUMEN
In 1813, Vautier published his observation of tumor regression in patients who had suffered from gas gangrene. Since then, many publications have described the use of bacteria as antitumor therapy. For example, Bifidobacterium and Clostridium have been shown to selectively colonize tumors and to reduce tumor size. In addition, recent studies have focused on the use of genetic engineering to induce the expression of pro-drug converting enzymes, cytokines, specific antibodies, or suicide genes in tumor-colonizing bacteria. Moreover, some animal experiments have reported the treatment of tumors with engineered bacteria, and few side effects were observed. Therefore, based on these advances in tumor targeting therapy, bacteria may represent the next generation of cancer therapy.
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Bifidobacterium/crecimiento & desarrollo , Terapia Biológica/métodos , Clostridium/crecimiento & desarrollo , Neoplasias/microbiología , Neoplasias/terapia , Animales , Bifidobacterium/metabolismo , Clostridium/metabolismo , Modelos Animales de Enfermedad , Humanos , Ingeniería MetabólicaRESUMEN
Avocado fruit extracts are known to exhibit antimicrobial properties. However, the effects on bacterial endospores and the identity of antimicrobial compounds have not been fully elucidated. In this study, avocado seed extracts were tested against Clostridium sporogenes vegetative cells and active endospores. Bioassay-guided purification of a crude extract based on inhibitory properties linked antimicrobial action to six lipid derivatives from the family of acetogenin compounds. Two new structures and four compounds known to exist in nature were identified as responsible for the activity. Structurally, most potent molecules shared features of an acetyl moiety and a trans-enone group. All extracts produced inhibition zones on vegetative cells and active endospores. Minimum inhibitory concentrations (MIC) of isolated molecules ranged from 7.8 to 15.6 µg/mL, and bactericidal effects were observed for an enriched fraction at 19.5 µg/mL. Identified molecules showed potential as natural alternatives to additives and antibiotics used by the food and pharmaceutical industries to inhibit Gram-positive spore-forming bacteria.
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Clostridium/efectos de los fármacos , Lípidos/química , Lípidos/farmacología , Persea/química , Extractos Vegetales/química , Extractos Vegetales/farmacología , Semillas/química , Esporas Bacterianas/crecimiento & desarrollo , Clostridium/crecimiento & desarrollo , Regulación hacia Abajo/efectos de los fármacos , Lípidos/aislamiento & purificación , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Extractos Vegetales/aislamiento & purificación , Esporas Bacterianas/efectos de los fármacosRESUMEN
Eleutherine americana extract and its oligosaccharides extract were demonstrated for their growth-promoting activities on mixed infant intestinal microbiota and individual bacterial species including Bacteroides, bifidobacteria, clostridia, and lactobacilli. Fermentation of all the substrates resulted in about 1-2 log increase in the numbers of bifidobacteria belonging to the dominant infant gut microbiota. The highest levels of the bacterial populations were resulted from the oligosaccharides extract. The oligosaccharides extract stimulated the growth of bifidobacteria in mixed and individual populations by increasing their numbers from 9.63 to 12.08 and 5.80 to 8.85 log cfu/ml, respectively within 48 h. In addition, Bacteroides populations were enhanced by fermentation of E. americana extract. Increase in lactobacilli level was observed from fermentation by a single bacterial species, but not from the mixed cultures. Fermentation of the extract, oligosaccharides extract, and commercial fructo-oligosaccharides by both mixed and individual intestinal microbiota resulted in increase in the production of short chain fatty acids. Acetic acid production was predominant, followed by lactic acid and minor amount of propionic and butyric acids. The highest production of acetic acid was resulted from the oligosaccharides extract. Increase in the acetic acid in mixed and individual species of bifidobacterial populations ranged from 1.21 to 34.26 and 1.02-25.21 mM, respectively. This study showed that E. americana can be considered as a potential prebiotic which may be supplemented as an ingredient in functional foods.
Asunto(s)
Intestinos/microbiología , Iridaceae/química , Metagenoma/fisiología , Oligosacáridos/metabolismo , Prebióticos , Bacteroides/efectos de los fármacos , Bacteroides/crecimiento & desarrollo , Bifidobacterium/efectos de los fármacos , Bifidobacterium/crecimiento & desarrollo , Clostridium/crecimiento & desarrollo , Ácidos Grasos Volátiles/metabolismo , Heces/microbiología , Fermentación , Humanos , Lactante , Lactobacillus/efectos de los fármacos , Lactobacillus/crecimiento & desarrollo , Oligosacáridos/farmacología , Extractos Vegetales/química , Extractos Vegetales/metabolismoAsunto(s)
Antioxidantes/farmacología , Extractos Vegetales/farmacología , Prebióticos , Selenio/farmacología , Té/química , Animales , Antibacterianos/farmacología , Antineoplásicos/farmacología , Bacteroides/efectos de los fármacos , Bacteroides/crecimiento & desarrollo , Bifidobacterium/efectos de los fármacos , Bifidobacterium/crecimiento & desarrollo , Clostridium/efectos de los fármacos , Clostridium/crecimiento & desarrollo , Glucuronidasa/antagonistas & inhibidores , Glucuronidasa/metabolismo , Humanos , Lactobacillaceae/efectos de los fármacos , Lactobacillaceae/crecimiento & desarrollo , RatasRESUMEN
UNLABELLED: The effects of red meat consumption with and without fermentable carbohydrates on indices of large bowel health in rats were examined. Sprague-Dawley rats were fed cellulose, potato fiber, or potato-resistant starch diets containing 12% casein for 2 wk, then similar diets containing 25% cooked beef for 6 wk. After week 8, cecal and colonic microbiota composition, fermentation end-products, colon structure, and colonocyte DNA damage were analyzed. Rats fed potato fiber had lower Bacteroides-Prevotella-Porphyromonas group compared to other diet groups. Colonic Bifidobacterium spp. and/or Lactobacillus spp. were higher in potato fiber and potato-resistant starch diets than in the cellulose diet. Beneficial changes were observed in short-chain fatty acid concentrations (acetic, butyric, and propionic acids) in rats fed potato fiber compared with rats fed cellulose. Phenol and p-cresol concentrations were lower in the cecum and colon of rats fed potato fiber. An increase in goblet cells per crypt and longer crypts were found in the colon of rats fed potato fiber and potato-resistant starch diets. Fermentable carbohydrates had no effect on colonic DNA damage. Dietary combinations of red meat with potato fiber or potato-resistant starch have distinctive effects in the large bowel. Future studies are essential to examine the efficacy of different types of nondigestible carbohydrates in maintaining colonic health during long-term consumption of high-protein diets. PRACTICAL APPLICATION: Improved understanding of interactions between the food consumed and gut microbiota provides knowledge needed to make healthier food choices for large bowel health. The impact of red meat on large bowel health may be ameliorated by consuming with fermentable dietary fiber, a colonic energy source that produces less harmful by-products than the microbial breakdown of colonic protein for energy. Developing functional red meat products with fermentable dietary fiber could be one way to promote a healthy and balanced macronutrient diet.