RESUMEN
Leishmaniasis is one of the most serious worldwide diseases caused by protozoan parasites of the Leishmania genus, affecting millions of people around the world. All currently available treatments present severe toxic side effects, require long-term compliance, cause serious side effects and are uncomfortable for patients. Leishmania amazonensis, a species endemic to Brazil, causes severe localised or diffuse skin lesions in humans. Owing to the unsatisfactory nature of the currently available chemotherapies, new approaches have been assessed for improved therapeutic intervention strategies against leishmaniasis. Miltefosine is an alkylphospholipid analogue that exhibits potent activity against the different clinical manifestations of leishmaniasis. Thus, the aim of this study was to investigate the long-term efficacy of miltefosine in BALB/c mice infected with L. amazonensis owing to the lack of a profound study demonstrating its dose-dependent and long-term effects. It was observed that animals treated with 20-50 mg/kg/day of miltefosine exhibited a significant dose-dependent reduction in lesion size; furthermore, in mice receiving higher doses, lesions disappeared after the end of treatment. To confirm a possible parasitological cure, mice up to 250 days after the end of treatment were analysed. No lesions or presence of parasite DNA were found in mice treated with 30, 40 and 50 mg/kg/day of miltefosine. In summary, these results show that miltefosine may be used to treat cutaneous leishmaniasis caused by L. amazonensis, alone or as combination therapy.
Asunto(s)
Antiprotozoarios/farmacología , Leishmania/patogenicidad , Leishmaniasis Cutánea/tratamiento farmacológico , Fosforilcolina/análogos & derivados , Animales , Antiprotozoarios/administración & dosificación , Colorantes Azulados/química , ADN Protozoario/química , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Femenino , Leishmania/química , Leishmania/genética , Leishmaniasis Cutánea/parasitología , Meglumina/farmacología , Antimoniato de Meglumina , Ratones , Ratones Endogámicos BALB C , Compuestos Organometálicos/farmacología , Carga de Parásitos , Fosforilcolina/administración & dosificación , Fosforilcolina/farmacología , Factores de Tiempo , Úlcera/tratamiento farmacológico , Úlcera/parasitologíaRESUMEN
A simple, accurate and rapid spectrophotmeric method was proposed for the determination of chondroitin sulfate. The method was based on the absorbances of AA-CS complex at 625 nm being in proportion to the chondroitin sulfate concentrations. The linear range was 0-30 micrograms.mL-1 (R = 0.999), and the recovery range was 97.4%-103.8%. The quantities of chondroitin sulfate in different sample were determined in this way.