RESUMEN
Surface modification of superparamagnetic Fe3O4 nanoparticles using polymers (polyaniline/polypyrrole) was done by radio frequency (r.f.) plasma polymerization technique and characterized by XRD, TEM, TG/DTA and VSM. Surface-passivated Fe3O4 nanoparticles with polymers were having spherical/rod-shaped structures with superparamagnetic properties. Broad visible photoluminescence emission bands were observed at 445 and 580 nm for polyaniline-coated Fe3O4 and at 488 nm for polypyrrole-coated Fe3O4. These samples exhibit good fluorescence emissions with L929 cellular assay and were non-toxic. Magnetic hyperthermia response of Fe3O4 and polymer (polyaniline/polypyrrole)-coated Fe3O4 was evaluated and all the samples exhibit hyperthermia activity in the range of 42-45 °C. Specific loss power (SLP) values of polyaniline and polypyrrole-coated Fe3O4 nanoparticles (5 and 10 mg/ml) exhibit a controlled heat generation with an increase in the magnetic field.
Asunto(s)
Compuestos de Anilina/química , Diagnóstico por Imagen/métodos , Hipertermia Inducida/métodos , Nanopartículas de Magnetita/química , Polímeros/química , Pirroles/química , Compuestos de Anilina/efectos de la radiación , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Compuestos Férricos/síntesis química , Compuestos Férricos/química , Compuestos Férricos/efectos de la radiación , Humanos , Campos Magnéticos , Magnetismo/métodos , Nanopartículas de Magnetita/efectos de la radiación , Nanopartículas de Magnetita/uso terapéutico , Ensayo de Materiales , Gases em Plasma/química , Polímeros/efectos de la radiación , Pirroles/efectos de la radiación , Ondas de Radio , Propiedades de Superficie/efectos de la radiación , Difracción de Rayos XRESUMEN
Iron oxide nanoparticles (FeONPs) prepared with plant extracts have been emerging as green and sustainable materials. FeONPs are usually amorphous due to the chelation of the tea polyphenols (TPs) to the iron, and the real nature of the iron compounds is not completely understood. The main goal of this study was to investigate the behavior of the green FeONPs synthesized from an Fe3+ salt and Cammelia sinensis (black tea) extract upon thermal treatment, in order to remove TPs and enable the formation of crystalline materials suitable for a thorough characterization and with the potential for diverse applications. The as-prepared FeONPs were assigned as mixed-valence Fe(III) oxyhydroxides and Fe(II)/Fe(III) ions bound to TPs. A detailed description of the phase transformation upon heating revealed the formation of the rare nano ß-Fe2O3 phase at 400 °C, followed by a transformation to α-Fe2O3 as the temperature increased. Above 600 °C, the unprecedented formation of FePO4 and Fe3PO7 was observed, produced from the reaction of Fe2O3 and free phosphate ions present in the black tea leaves, Fe3PO7 being the major phase obtained at 900 °C. Finally, the catalytic potential of the FeONPs to treat the azo dye methyl orange through a heterogeneous Fenton-like system was investigated.
Asunto(s)
Camellia sinensis/química , Compuestos Férricos/química , Fosfatos/síntesis química , Extractos Vegetales/química , Polifenoles/química , Compuestos Férricos/síntesis química , Tamaño de la Partícula , Fosfatos/química , TemperaturaRESUMEN
Magnetic nanoparticles such as cobalt ferrite are investigated under clinical hyperthermia conditions for the treatment of cancer. Cobalt ferrite nanoparticles (CFNPs) synthesized by the thermal decomposition method, using nonionic surfactant Triton-X100, possess hydrophilic polyethylene oxide chains acting as reducing agents for the cobalt and iron precursors. The monodispersed nanoparticles were of 10 nm size, as confirmed by high-resolution transmission electron microscopy (HR-TEM). The X-ray diffraction patterns of CFNPs prove the existence of cubic spinel cobalt ferrites. Cs-corrected scanning transmission electron microscopy-high-angle annular dark-field imaging (STEM-HAADF) of CFNPs confirmed their multi-twinned crystallinity due to the presence of atomic columns and defects in the nanostructure. Magnetic measurements proved that the CFNPs possess reduced remnant magnetization (MR/MS) (0.86), which justifies cubic anisotropy in the system. Microwave-based hyperthermia studies performed at 2.45 GHz under clinical conditions in physiological saline increased the temperature of the CFNP samples due to the transformation of radiation energy to heat. The specific absorption rate of CFNPs in physiological saline was 68.28 W/g. Furthermore, when triple-negative breast cancer cells (TNBC) in the presence of increasing CFNP concentration (5 mg/mL to 40 mg/mL) were exposed to microwaves, the cell cytotoxicity was enhanced compared to CFNPs alone.
Asunto(s)
Antineoplásicos , Cobalto , Compuestos Férricos , Hipertermia Inducida , Campos Magnéticos , Nanopartículas , Neoplasias de la Mama Triple Negativas/terapia , Antineoplásicos/síntesis química , Antineoplásicos/química , Antineoplásicos/farmacología , Línea Celular Tumoral , Cobalto/química , Cobalto/farmacología , Femenino , Compuestos Férricos/síntesis química , Compuestos Férricos/química , Compuestos Férricos/farmacología , Humanos , Nanopartículas/química , Nanopartículas/uso terapéuticoRESUMEN
A method is described for the functionalization of magnetic carbon nanotubes to recognize aristolochic acid â and â ¡. 3-Glycidyloxypropyltrimethoxysilane was used as a coupling agent to immobilize adenine on a solid support. The morphology and structure of adenine-coated magnetic carbon nanotubes was investigated using transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and a vibrating sample magnetometer (VSM). The adsorption performance of the adenine-coated magnetic carbon nanotubes was evaluated via adsorption isotherms, the kinetics and selectivity tests. The adsorption capacity of the adenine-functionalized sorbent for aristolochic acid â was determined to be 24.5 µg mg-1. By combining magnetic solid phase extraction with HPLC detection, a method was developed to enrich and detect aristolochic acids used in traditional Chinese medicine. A satisfactory recovery (92.7 - 97.5% for aristolochic acid â and 92.6 - 99.4% for aristolochic acid â ¡) and an acceptable relative standard deviation (<4.0%) were obtained.
Asunto(s)
Adenina/química , Ácidos Aristolóquicos/aislamiento & purificación , Fenómenos Magnéticos , Nanotubos de Carbono/química , Adsorción , Medicamentos Herbarios Chinos/química , Compuestos Férricos/síntesis química , Compuestos Férricos/química , Concentración de Iones de Hidrógeno , Cinética , Nanocompuestos/química , Nanotubos de Carbono/ultraestructura , Concentración Osmolar , Reproducibilidad de los Resultados , Dióxido de Silicio/síntesis química , Dióxido de Silicio/química , Extracción en Fase Sólida , Temperatura , Difracción de Rayos XRESUMEN
CuO/MgAl2O4 and CuO/MgFe2O4 catalysts were successfully synthesized with the use of spinel supports by a very simple and low-cost mechanochemical method. High-speed ball-milling was used to synthesize these catalyst supports for the first time. Materials were subsequently characterized by using XRD, FESEM, TEM, EDS-Dot mapping, XPS, BET-BJH, and Magnetic Susceptibility to investigate the physical-chemical characteristics of the catalysts. Acidity evaluation results indicated that the catalyst with the Mg-Al spinel support had more acid sites. XRD results showed a successful synthesis of the catalysts with large crystal sizes. Both catalysts were used in isoeugenol oxidation and vanillyl alcohol to vanillin reactions, with the CuO/MgAl2O4 showing optimum results. This catalyst provided 67% conversion (74% selectivity) after 2 h and this value improved to 81% (selectivity 100%) with the second reaction after 8 h. The CuO/MgFe2O4 catalyst in the first reaction after five hours revealed 53% conversion (47% selectivity) and after eight hours with the second reaction, the conversion value improved to 64% (100% selectivity). In terms of reusability, CuO/MgAl2O4 showed better results than the CuO/MgFe2O4 catalyst, for both reactions.
Asunto(s)
Óxido de Aluminio/química , Benzaldehídos/síntesis química , Compuestos Férricos/química , Compuestos de Magnesio/química , Óxido de Magnesio/química , Óxido de Aluminio/síntesis química , Benzaldehídos/química , Alcoholes Bencílicos/química , Catálisis , Cobre/química , Eugenol/análogos & derivados , Eugenol/química , Compuestos Férricos/síntesis química , Compuestos de Magnesio/síntesis química , Óxido de Magnesio/síntesis química , Oxidación-ReducciónRESUMEN
Recently, the intrinsic enzyme-like activities of some nanoscale materials known as "nanozymes" have become a growing area of interest. Nanosized spinel substituted ferrites (SFs) with general formula of MFe2O4, where M represents a transition metal, are among a group of magnetic nanomaterials attracting researchers' enormous attention because of their excellent catalytic performance, biomedical applications and capability for environmental remediation. Due to their unique nanoscale physical-chemical properties, they have been used to mimic the catalytic activity of natural enzymes such as peroxidases, oxidases and catalases. In addition, various nanocomposite materials based on SFs have been introduced as novel artificial enzymes. This review mainly highlights the synthetic approaches for newly developed SF-nanozymes and also the structural/experimental factors that are effective on the kinetics and catalytic mechanisms of enzyme-like reactions. SF-nanozymes have been found potentially capable of being applied in various fields such as enzyme-free immunoassays and biosensors for colorimetric detection of biological molecules. Therefore, the application of SF nanoparticles, as efficient enzyme mimetics have been detailed discussed.
Asunto(s)
Óxido de Aluminio/química , Materiales Biomiméticos/síntesis química , Enzimas/metabolismo , Compuestos Férricos/síntesis química , Óxido de Magnesio/química , Nanoestructuras/química , Materiales Biomiméticos/química , Técnicas Biosensibles , Compuestos Férricos/químicaRESUMEN
Poor O2 supply to the infiltrated immune cells in the joint synovium of rheumatoid arthritis (RA) up-regulates hypoxia-inducible factor (HIF-1α) expression and induces reactive oxygen species (ROS) generation, both of which exacerbate synovial inflammation. Synovial inflammation in RA can be resolved by eliminating pro-inflammatory M1 macrophages and inducing anti-inflammatory M2 macrophages. Because hypoxia and ROS in the RA synovium play a crucial role in the induction of M1 macrophages and reduction of M2 macrophages, herein, we develop manganese ferrite and ceria nanoparticle-anchored mesoporous silica nanoparticles (MFC-MSNs) that can synergistically scavenge ROS and produce O2 for reducing M1 macrophage levels and inducing M2 macrophages for RA treatment. MFC-MSNs exhibit a synergistic effect on O2 generation and ROS scavenging that is attributed to the complementary reaction of ceria nanoparticles (NPs) that can scavenge intermediate hydroxyl radicals generated by manganese ferrite NPs in the process of O2 generation during the Fenton reaction, leading to the efficient polarization of M1 to M2 macrophages both in vitro and in vivo. Intra-articular administration of MFC-MSNs to rat RA models alleviated hypoxia, inflammation, and pathological features in the joint. Furthermore, MSNs were used as a drug-delivery vehicle, releasing the anti-rheumatic drug methotrexate in a sustained manner to augment the therapeutic effect of MFC-MSNs. This study highlights the therapeutic potential of MFC-MSNs that simultaneously generate O2 and scavenge ROS, subsequently driving inflammatory macrophages to the anti-inflammatory subtype for RA treatment.
Asunto(s)
Acetatos/farmacología , Artritis Reumatoide/tratamiento farmacológico , Cerio/farmacología , Compuestos Férricos/farmacología , Compuestos de Manganeso/farmacología , Nanopartículas/química , Acetatos/síntesis química , Acetatos/química , Animales , Artritis Reumatoide/inducido químicamente , Artritis Reumatoide/metabolismo , Supervivencia Celular/efectos de los fármacos , Cerio/química , Modelos Animales de Enfermedad , Compuestos Férricos/síntesis química , Compuestos Férricos/química , Adyuvante de Freund , Masculino , Compuestos de Manganeso/síntesis química , Compuestos de Manganeso/química , Oxígeno/metabolismo , Tamaño de la Partícula , Ratas , Ratas Sprague-Dawley , Especies Reactivas de Oxígeno/metabolismo , Propiedades de SuperficieRESUMEN
Green synthesis of nanoparticles is one of the promising, ecofriendly and safer methods. Utilizing plant sources as reducing agents will replace the use of toxic chemicals for nanoparticle synthesis. In the present study FeONPs were synthesized using Ruellia tuberosa (RT) leaf aqueous extract, further characterization of FeONPs was performed using UV-vis spectroscopy analysis showing visible peak at 405â¯nm. The Fourier transform infrared spectroscopy (FTIR) proved the presence of Fe metallic ions. The structural characteristic using Field emission scanning electron microscopy with energy dispersive x-ray spectroscopy (FESEM-EDX) and Transmission electron microscopy (TEM) analysis revealed hexagonal nanorods with agglomeration. Dynamic light scattering (DLS) calculated the average size of FeONPs around 52.78â¯nm and differential scanning colorimetry (DSC) proved the stability of FeONPs till higher temperature of 165.52⯰C. As an application part, the synthesized FeONPs showed potential antibacterial activity as individual and incorporating material over cotton fabrics against Gram negative and Gram positive pathogens. FeONPs showed higher antibacterial activity against Escherichia coli, Klebsiella pneumoniae and lesser antibacterial activity against Staphylococcus aureus. The photocatalytic ability of the synthesized FeONPs was demonstrated by the degrading crystal violet dye under solar irradiation upto 80%. Thus, FeONPs synthesized using Ruellia tuberosa could play a vital role in killing the bacterial pathogens and degrading dye for the bioremediation of wastewater from industrial and domestic sources.
Asunto(s)
Acanthaceae/química , Antibacterianos/farmacología , Compuestos Férricos/síntesis química , Nanopartículas del Metal/química , Fotólisis/efectos de los fármacos , Extractos Vegetales/química , Biodegradación Ambiental , Catálisis/efectos de los fármacos , Colorantes/química , Escherichia coli/efectos de los fármacos , Tecnología Química Verde , Klebsiella pneumoniae/efectos de los fármacos , Aguas Residuales/químicaRESUMEN
The present study explored the one step extracellular green synthesis of Iron oxide (FexOy) and manganese oxide nanoparticles (MnNPs) using aqueous extract of Acorus calamus rhizome. The organic chemicals including polyphenol compounds responsible for bio-reduction and stabilization from the polyphenol enriched microwave irradiated aqueous extract of Acorus calamus were studied using GC-MS analysis. Further, their synthesis conditions were optimized using response surface methodology (RSM) and central composite design (CCD) using three variables. The green synthesized Iron oxide and Manganese oxide NPs were characterized by UV, FTIR, XRD, TEM and SEM. Results indicated that the Iron oxide NPs and mixture of iron and manganese NPs showed photocatalytic excellent activities in reducing dyes like methylene blue (0.1%) and Congo red (0.25%) at 0.03% NPs. However, Mn NPs showed moderate activity. On a contrary, manganese showed better larvicidal activity compared to Iron oxide NPs against the phytopathogens commonly affecting the vegetable crops. The present finding showed that high mortality rate at 30⯵g/ml concentration of manganese NPs was comparatively interesting. In addition, NPs overall had appreciable activity with P. aeruginosa being more sensitive to Iron oxide NPs (22⯱â¯2â¯mm zone of inhibition) and manganese NPs (13⯱â¯2â¯mm zone of inhibition) and Iron oxide NPs completely inhibited the growth of A. flavus at 40⯵g/ml concentration.
Asunto(s)
Acoraceae/química , Antibacterianos/síntesis química , Compuestos Azo/química , Tecnología Química Verde/métodos , Insecticidas/síntesis química , Aspergillus flavus/efectos de los fármacos , Compuestos Férricos/síntesis química , Compuestos de Manganeso/síntesis química , Nanopartículas/química , Nanopartículas/uso terapéutico , Óxidos/síntesis química , Extractos Vegetales/química , Polifenoles/análisis , Pseudomonas aeruginosa/efectos de los fármacosRESUMEN
Ficus carica (common fig) dried fruit extract was used to synthesize iron oxide nanoparticles in this study. Biomaterials in the common fig dried fruit extract synthesized the iron nanoparticles by reducing the iron precursor salt and then acted as capping and stabilizing agents. The nanoparticles were produced smaller than 20 nm diameters and oxidized due to the high phenolic compound content in the common fig dried fruit extract. Nanoparticles were characterized by energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS). First, color change and pH reduction occurred immediately due to the iron nanoparticle synthesis. TEM images showed that the nanoparticles were at 9 ± 4 nm diameters and the metallic core-oxide shell form. The nanoparticles were in spherical shapes with a monodisperse distribution. EDX, XRD and FTIR analysis signals showed the iron oxyhydroxide/oxide formation. Absorption peaks were detected at 205 nm and 291 nm due to the iron metallic core hydrolysis products. The intensity-average diameter of nanoparticles was calculated at 475 nm diameter by DLS analysis. Colloid stability was determined as moderate at 20.7 mV.
Asunto(s)
Compuestos Férricos/química , Compuestos Férricos/síntesis química , Ficus/química , Tecnología Química Verde/métodos , Nanopartículas del Metal/química , Extractos Vegetales/química , Biocatálisis , Coloides/síntesis química , Coloides/química , Desecación , Frutas/química , Microscopía Electrónica de Transmisión , Tamaño de la Partícula , Espectroscopía Infrarroja por Transformada de Fourier , Difracción de Rayos XRESUMEN
A new folic acid (FA)-conjugated poly (lactic-co-glycolicacid) (PLGA)-polyethylene glycol (PEG) nano-noisome was prepared. The noisome was employed as a drug delivery system to load curcumin (Cur) as a model drug and fluorescent probe for cervical cancer therapy and cell imaging. The Fe3O4@PLGA-PEG@FA noisomes were prepared through facile emulsion solvent evaporation and conjugation chemistry method, possessing the properties of high rapid magnetic separation and targeting character. X-ray photoelectron spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM) were adopted to characterize the chemical structure and properties of these niosomes. MTT assay revealed that the blank noisomes exhibited excellent biocompatibility. The in vitro drug loading and release behavior studier showed the as prepared nano-noisome presented ultrahigh performance as drug carrier. The confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) experiments demonstrated that Cur-loaded Fe3O4@PLGA-PEG@FA niosomes achieved significantly high targeting efficiency for cervical cancer. Additionally, the FA-targeted niosomes exhibited higher antitumor efficiency than free Cur. Cell morphology, the mitochondrial membrane potential and cell cycle changes indicated that Cur-loaded niosomes induced HeLa229 cells to apoptosis by destroying mitochondrion of cervical tumor cells, simultaneously changing nuclear morphology and blocking tumor cell proliferation. These results demonstrate that Fe3O4@PLGA-PEG@FA noisomes have promising applications as targeted drug delivery system for sustained drug release in cancer treatment.
Asunto(s)
Portadores de Fármacos/química , Compuestos Férricos/síntesis química , Liposomas/química , Nanopartículas/química , Polietilenglicoles/síntesis química , Poliglactina 910/síntesis química , Neoplasias del Cuello Uterino/tratamiento farmacológico , Animales , Apoptosis , Ciclo Celular , Dispersión Dinámica de Luz , Femenino , Células HeLa , Humanos , Nanopartículas/ultraestructura , Polietilenglicoles/química , Poliglactina 910/química , Conejos , Espectrometría de Fluorescencia , Difracción de Rayos XRESUMEN
Aqueous floral extracts of Callistemon viminalis were used to synthesize Fe2O3 nanoparticles (IONPs) which were intensively characterized through UV-vis, X-ray diffraction, HR-SEM/HR-TEM, Fourier- transform infrared spectroscopy (FTIR) and energy dispersive X-ray spectroscopy (EDS). Their physical properties were studied in response to different annealing temperatures. It was observed that the increase in the annealing temperature produced small-sized nanoparticles. The nanoparticle size was calculated as 32, 26 and 22 nm for annealing at 300, 400 and 500 °C, respectively. The magnetic nature of the bioinspired IONPs was revealed by superconducting quantum interference device (SQUID). Their antibacterial potential was investigated against nine pathogenic bacterial strains (gram positive and gram negative) using disc diffusion method while their MIC was calculated using broth dilution assay. Bioinspired IONPs were found to be highly effective against HepG2 cells (IC50=20 µg/mL). Moderate antileishmanial activities against the promastigotes and amastigotes cultures are reported. Moderate acetylcholine esterase (AchE), butylcholine esterase (BchE) and α-Glycosidase inhibition are reported. Additional assessment of the biocompatibility was performed using haemolytic activity on the freshly isolated human red blood cells and macrophages. Furthermore, the antioxidant activities, including TAC, DPPH and TRP were also performed. Our results indicate that the biogenic and magnetic Fe2O3 can be used for diverse biomedical applications.
Asunto(s)
Compuestos Férricos/síntesis química , Compuestos Férricos/farmacología , Flores/química , Imanes/química , Myrtaceae/química , Fenómenos Físicos , Extractos Vegetales/química , Antibacterianos/síntesis química , Antibacterianos/química , Antibacterianos/farmacología , Antineoplásicos/síntesis química , Antineoplásicos/química , Antineoplásicos/farmacología , Antioxidantes/síntesis química , Antioxidantes/química , Antioxidantes/farmacología , Antiprotozoarios/síntesis química , Antiprotozoarios/química , Antiprotozoarios/farmacología , Técnicas de Química Sintética , Inhibidores de la Colinesterasa/síntesis química , Inhibidores de la Colinesterasa/química , Inhibidores de la Colinesterasa/farmacología , Eritrocitos/citología , Eritrocitos/efectos de los fármacos , Compuestos Férricos/química , Células Hep G2 , Humanos , Ensayo de Materiales , Tamaño de la Partícula , TemperaturaRESUMEN
Co-precipitation method was used for preparation of two types of iron oxide nanoparticles coated by titanium dioxide according to divalent salts used. The average size of iron oxide nanoparticles coated by titanium dioxide measured by particle size analyzer, ranged approximately between 20 nm and 100 nm with mean particle size of 60 nm. Characterization of the prepared nanoparticles was done by X-ray diffraction analysis and scanning electron microscope indicating the sole existence of inverse cubic spinel phase of magnetic iron oxide nanoparticles. Further, the antibacterial activity of two prepared iron oxide nanoparticles was evaluated against four pathogenic bacteria where both preparations showed promising antibacterial activities against Gram positive and Gram negative strains which offers a potential application in pharmaceutical and biomedical industries. The antibacterial activity showed high reduction percent after 30 min by 150 µg mL-1 of nanoparticles prepared. Also, high reduction percent was achieved for removal of iron and manganese ions from polluted water and good effect on decreasing chemical oxygen demand and biochemical oxygen demand concentrations with decreased percent of total nitrogen concentration.
Asunto(s)
Antibacterianos/farmacología , Nanopartículas del Metal/química , Titanio/química , Adsorción , Antibacterianos/química , Bacterias/efectos de los fármacos , Evaluación Preclínica de Medicamentos , Compuestos Férricos/síntesis química , Compuestos Férricos/química , Compuestos Férricos/farmacología , Tamaño de la Partícula , Contaminantes Químicos del Agua/química , Difracción de Rayos XRESUMEN
Magnetic hyperthermia (MH) based on magnetic nanoparticles (MNPs) is a promising adjuvant therapy for cancer treatment. Particle clustering leading to complex magnetic interactions affects the heat generated by MNPs during MH. The heat efficiencies, theoretically predicted, are still poorly understood because of a lack of control of the fabrication of such clusters with defined geometries and thus their functionality. This study aims to correlate the heating efficiency under MH of individually coated iron oxide nanocubes (IONCs) versus soft colloidal nanoclusters made of small groupings of nanocubes arranged in different geometries. The controlled clustering of alkyl-stabilized IONCs is achieved here during the water transfer procedure by tuning the fraction of the amphiphilic copolymer, poly(styrene-co-maleic anhydride) cumene-terminated, to the nanoparticle surface. It is found that increasing the polymer-to-nanoparticle surface ratio leads to the formation of increasingly large nanoclusters with defined geometries. When compared to the individual nanocubes, we show here that controlled grouping of nanoparticles-so-called "dimers" and "trimers" composed of two and three nanocubes, respectively-increases specific absorption rate (SAR) values, while conversely, forming centrosymmetric clusters having more than four nanocubes leads to lower SAR values. Magnetization measurements and Monte Carlo-based simulations support the observed SAR trend and reveal the importance of the dipolar interaction effect and its dependence on the details of the particle arrangements within the different clusters.
Asunto(s)
Compuestos Férricos/química , Hipertermia Inducida , Nanopartículas de Magnetita/química , Neoplasias/tratamiento farmacológico , Quimioterapia Adyuvante , Coloides/química , Compuestos Férricos/síntesis química , Compuestos Férricos/uso terapéutico , Humanos , Nanopartículas de Magnetita/uso terapéutico , Simulación de Dinámica Molecular , Estructura Molecular , Método de Montecarlo , Tamaño de la Partícula , Propiedades de SuperficieRESUMEN
ABSTARCT: This work explores a new class of vortex/magnetite/iron oxide nanoparticles designed for magnetic hyperthermia applications. These nanoparticles, named Vortex Iron oxide Particles (VIPs), are an alternative to the traditional Superparamagnetic Iron Oxide Nanoparticles (SPIONs), since VIPs present superior heating power while fulfilling the main requirements for biomedical applications (low cytotoxicity and nonremanent state). In addition, the present work demonstrates that the synthesized VIPs also promote an internalization and aggregation of the particles inside the cell, resulting in a highly localized hyperthermia in the presence of an alternating magnetic field. Thereby, we demonstrate a new and efficient magnetic hyperthermia strategy in which a small, but well localized, concentration of VIPs can promote an intracellular hyperthermia process.
Asunto(s)
Hipertermia Inducida/métodos , Nanopartículas de Magnetita/uso terapéutico , Neoplasias/terapia , Supervivencia Celular , Compuestos Férricos/síntesis química , Células HEK293 , Humanos , Campos Magnéticos , Magnetismo , Nanopartículas de Magnetita/química , MicroscopíaRESUMEN
The present work deals with the ultrasound assisted green synthesis of iron oxide nano particle using Coriandrum sativum leaf extract as a reducing agent. The synthesized iron oxide nanoparticle was confirmed by UV spectra. The characterization was done to know more about morphology and size of the particle by SEM analysis which shows spherical particles with size ranging from 20 to 90nm. The antimicrobial activity of the leaf extract and the synthesized nanoparticles was studied against the pathogens Micrococcus luteus, Staphylococcus aureus and Aspergillus niger. The ultrasound assisted iron oxide nanoparticle shows higher scavenging activity and antimicrobial activity compared with iron oxide nanoparticle synthesized by magnetic stirrer and Coriandrum sativum leaf extract.
Asunto(s)
Coriandrum/química , Compuestos Férricos/química , Compuestos Férricos/síntesis química , Nanopartículas , Extractos Vegetales/química , Ondas Ultrasónicas , Antiinfecciosos/síntesis química , Antiinfecciosos/química , Antiinfecciosos/farmacología , Antioxidantes/síntesis química , Antioxidantes/química , Antioxidantes/farmacología , Técnicas de Química Sintética , Compuestos Férricos/farmacología , Nanotecnología , Hojas de la Planta/químicaRESUMEN
A multifunctional magneto-plasmonic CoFe2O4@Au core-shell nanoparticle was developed by iterative-seeding based method. This nanocargo consists of a cobalt ferrite kernel as a core (Nk) and multiple layers of gold as a functionalizable active stratum, (named as Nk@A after fifth iteration). Nk@A helps in augmenting the physiological stability and enhancing surface plasmon resonance (SPR) property. The targeted delivery of Doxorubicin using Nk@A as a nanopayload is demonstrated in this report. The drug release profile followed first order rate kinetics optimally at pH 5.4, which is considered as an endosomal pH of cells. The cellular MR imaging showed that Nk@A is an efficient T2 contrast agent for both L6 (r2-118.08 mM-1s-1) and Hep2 (r2-217.24 mM-1s-1) cells. Microwave based magnetic hyperthermia studies exhibited an augmentation in the temperature due to the transformation of radiation energy into heat at 2.45 GHz. There was an enhancement in cancer cell cytotoxicity when hyperthermia combined with chemotherapy. Hence, this single nanoplatform can deliver 3-pronged theranostic applications viz., targeted drug-delivery, T2 MR imaging and hyperthermia.
Asunto(s)
Medios de Contraste/química , Doxorrubicina/administración & dosificación , Nanopartículas del Metal/administración & dosificación , Nanomedicina Teranóstica/métodos , Antibióticos Antineoplásicos/administración & dosificación , Antibióticos Antineoplásicos/farmacocinética , Cobalto/química , Doxorrubicina/farmacocinética , Sistemas de Liberación de Medicamentos/métodos , Liberación de Fármacos , Compuestos Férricos/síntesis química , Compuestos Férricos/química , Ácido Fólico/química , Oro/química , Células Hep G2 , Humanos , Hipertermia Inducida/métodos , Imagen por Resonancia Magnética , Magnetismo/métodos , Microscopía Electrónica de Transmisión , Microondas , Nanomedicina Teranóstica/instrumentación , Difracción de Rayos XRESUMEN
Uniform wüstite Fe0.6 Mn0.4 O nanoflowers have been successfully developed as an innovative theranostic agent with T1 -T2 dual-mode magnetic resonance imaging (MRI), for diagnostic applications and therapeutic interventions via magnetic hyperthermia. Unlike their antiferromagnetic bulk counterpart, the obtained Fe0.6 Mn0.4 O nanoflowers show unique room-temperature ferromagnetic behavior, probably due to the presence of an exchange coupling effect. Combined with the flower-like morphology, ferromagnetic Fe0.6 Mn0.4 O nanoflowers are demonstrated to possess dual-modal MRI sensitivity, with longitudinal relaxivity r1 and transverse relaxivity r2 as high as 4.9 and 61.2 mm(-1) s(-1) [Fe]+[Mn], respectively. Further in vivo MRI carried out on the mouse orthotopic glioma model revealed gliomas are clearly delineated in both T1 - and T2 -weighted MR images, after administration of the Fe0.6 Mn0.4 O nanoflowers. In addition, the Fe0.6 Mn0.4 O nanoflowers also exhibit excellent magnetic induction heating effects. Both in vitro and in vivo magnetic hyperthermia experimentation has demonstrated that magnetic hyperthermia by using the innovative Fe0.6 Mn0.4 O nanoflowers can induce MCF-7 breast cancer cell apoptosis and a complete tumor regression without appreciable side effects. The results have demonstrated that the innovative Fe0.6 Mn0.4 O nanoflowers can be a new magnetic theranostic platform for in vivo T1 -T2 dual-mode MRI and magnetic thermotherapy, thereby achieving a one-stop diagnosis cum effective therapeutic modality in cancer management.
Asunto(s)
Neoplasias de la Mama , Medios de Contraste , Compuestos Férricos , Hipertermia Inducida/métodos , Imagen por Resonancia Magnética , Imanes/química , Compuestos de Manganeso , Nanopartículas , Óxidos , Neoplasias de la Mama/diagnóstico por imagen , Neoplasias de la Mama/patología , Neoplasias de la Mama/terapia , Medios de Contraste/síntesis química , Medios de Contraste/química , Medios de Contraste/farmacología , Femenino , Compuestos Férricos/síntesis química , Compuestos Férricos/química , Compuestos Férricos/farmacología , Humanos , Células MCF-7 , Compuestos de Manganeso/síntesis química , Compuestos de Manganeso/química , Compuestos de Manganeso/farmacología , Nanopartículas/química , Nanopartículas/uso terapéutico , Óxidos/síntesis química , Óxidos/química , Óxidos/farmacología , Nanomedicina Teranóstica/métodosRESUMEN
Magnetic nanoparticles (MNPs) have been widely investigated as a hyperthermic agent for cancer treatment. In this study, thermally responsive Chitosan-coated MnFe2O4 (Chitosan-MnFe2O4) nanoparticles were developed to conduct localized magnetic hyperthermia for cancer treatment. Hydrophobic MnFe2O4 nanoparticles were synthesized via thermal decomposition and modified with 2,3-dimercaptosuccinic acid (DMSA) for further conjugation of chitosan. Chitosan-MnFe2O4 nanoparticles exhibited high magnetization and excellent biocompatibility along with low cell cytotoxicity. During magnetic hyperthermia treatment (MHT) with Chitosan-MnFe2O4 on MDA-MB 231 cancer cells, the targeted therapeutic temperature was achieved by directly controlling the strength of the external AC magnetic fields. In vitro Chitosan-MnFe2O4-assisted MHT at 42 °C led to drastic and irreversible changes in cell morphology and eventual cellular death in association with the induction of apoptosis through heat dissipation from the excited magnetic nanoparticles. Therefore, the Chitosan-MnFe2O4 nanoparticles with high biocompatibility and thermal capability can be an effective nano-mediated agent for MHT on cancer.
Asunto(s)
Materiales Biocompatibles/química , Quitosano/química , Compuestos Férricos/farmacología , Hipertermia Inducida/métodos , Nanopartículas de Magnetita/química , Compuestos de Manganeso/farmacología , Apoptosis , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Compuestos Férricos/síntesis química , Humanos , Técnicas In Vitro , Compuestos de Manganeso/síntesis química , Neoplasias/terapiaRESUMEN
A green synthesis of iron oxide nanoparticles (FeNPs) was developed using Murraya koenigii leaf extract as reducing and stabilizing agent. UV-vis spectra show that the absorption band centred at a wavelength of 277 nm which corresponds to the surface plasmon resonances of synthesized FeNPs. Fourier transform infrared spectroscopy spectrum exhibits that the characteristic band at 580 cm(-1) is assigned to Fe-O of γ-Fe2O3. Transmission electron microscopy image confirms that the spherical with irregular shaped aggregates and average size of nanoparticles was found to be â¼59 nm. The effect of synthesized FeNPs on fermentative hydrogen production was evaluated from glucose by Clostridium acetobutylicum NCIM 2337. The hydrogen yield in control experiment was obtained as 1.74 ± 0.08 mol H2/mol glucose whereas the highest hydrogen yield in FeNPs supplemented experiment was achieved as 2.33 ± 0.09 mol H2/mol glucose at 175 mg/L of FeNPs. In addition, the hydrogen content and hydrogen production rate were also increased from 34 ± 0.8 to 52 ± 0.8 % and 23 to 25.3 mL/h, respectively. The effect of FeNPs was compared with supplementation of FeSO4 on fermentative process. The supplementation of FeNPs enhanced the hydrogen production in comparison with control and FeSO4. The supplementation of FeNPs led to the change of the metabolic pathway towards high hydrogen production due to the enhancement of ferredoxin activity. The fermentation type was shifted from butyrate to acetate/butyrate fermentation type at the addition of FeNPs.