RESUMEN
Paraquat is a green liquid toxin that is used in agriculture and can induce multi-organ including lung injury. Various pharmacological effects of Crocus sativus (C. sativus) were indicated in previous studies. In this research, the effects of C. sativus extract and pioglitazone on inhaled paraquat-induced lung inflammation, oxidative stress, pathological changes, and tracheal responsiveness were studied in rats. Eight groups of rats (n = 7 in each) including control (Ctrl), untreated paraquat aerosol exposed group (54 mg/m3, 8 times in alternate days), paraquat treated groups with dexamethasone (0.03 mg/kg/day, Dexa) as positive control, two doses of C. sativus extract (20 and 80 mg/kg/day, CS-20 and CS-80), pioglitazone (5 and 10 mg/kg/day, Pio-5 and Pio-10), and the combination of CS-20 + Pio-5 were studied. Total and differential WBC, levels of oxidant and antioxidant biomarkers in the BALF, lung tissue cytokine levels, tracheal responsiveness (TR), and pathological changes were measured. The levels of IFN-γ, IL-10, SOD, CAT, thiol, and EC50 were reduced, but MDA level, total and differential WBC count in the BALF and lung pathological changes were increased in the paraquat group (all, p < 0.001). The levels of IFN-γ, IL-10, SOD, CAT, thiol and EC50 were increased but BALF MDA level, lung pathological changes, total and differential WBC counts were reduced in all treated groups. The effects of C. sativus high dose and combination groups on measured parameters were equal or even higher than dexamethasone (p < 0.05 to p < 0.001). The effects of the combination of CS-20 + Pio-5 on most variables were significantly higher than CS-20 and Pio-5 alone (p < 0.05 to p < 0.001). C. sativus treatment improved inhaled paraquat-induced lung injury similar to dexamethasone and showed a synergistic effect with pioglitazone, suggesting possible PPAR-γ receptor-mediated effects of the plant.
Asunto(s)
Lesión Pulmonar Aguda , Crocus , Neumonía , Edema Pulmonar , Ratas , Animales , Paraquat/toxicidad , Paraquat/uso terapéutico , Crocus/metabolismo , Interleucina-10 , Pioglitazona/toxicidad , Pioglitazona/uso terapéutico , Neumonía/inducido químicamente , Neumonía/tratamiento farmacológico , Neumonía/patología , Inflamación/inducido químicamente , Inflamación/tratamiento farmacológico , Inflamación/patología , Pulmón , Edema Pulmonar/tratamiento farmacológico , Estrés Oxidativo , Lesión Pulmonar Aguda/inducido químicamente , Dexametasona/uso terapéutico , Superóxido Dismutasa/metabolismo , Compuestos de Sulfhidrilo/toxicidad , Compuestos de Sulfhidrilo/uso terapéuticoRESUMEN
BACKGROUND: Methyl mercaptan occurs naturally in the environment and is found in a variety of occupational settings, including the oil, paper, plastics, and pesticides industries. It is a toxic gas and deaths from methyl mercaptan exposure have occurred. The Department of Homeland Security considers it a high threat chemical agent that could be used by terrorists. Unfortunately, no specific treatment exists for methyl mercaptan poisoning. METHODS: We conducted a randomized trial in 12 swine comparing no treatment to intramuscular injection of the vitamin B12 analog cobinamide (2.0 mL, 12.5 mg/kg) following acute inhalation of methyl mercaptan gas. Physiological and laboratory parameters were similar in the control and cobinamide-treated groups at baseline and at the time of treatment. RESULTS: All six cobinamide-treated animals survived, whereas only one of six control animals lived (17% survival) (p = 0.0043). The cobinamide-treated animals returned to a normal breathing pattern by 3.8 ± 1.1 min after treatment (mean ± SD), while all but one animal in the control group had intermittent gasping, never regaining a normal breathing pattern. Blood pressure and arterial oxygen saturation returned to baseline values within 15 minutes of cobinamide-treatment. Plasma lactate concentration increased progressively until death (10.93 ± 6.02 mmol [mean ± SD]) in control animals, and decreased toward baseline (3.79 ± 2.93 mmol [mean ± SD]) by the end of the experiment in cobinamide-treated animals. CONCLUSION: We conclude that intramuscular administration of cobinamide improves survival and clinical outcomes in a large animal model of acute, high dose methyl mercaptan poisoning.
Asunto(s)
Antídotos/farmacología , Cobamidas/farmacología , Compuestos de Sulfhidrilo/toxicidad , Animales , Antídotos/administración & dosificación , Cobamidas/administración & dosificación , Femenino , Exposición por Inhalación , Inyecciones Intramusculares , Masculino , Distribución Aleatoria , PorcinosRESUMEN
Moderate exercise combined with proper nutrition are considered protective factors against cardiovascular disease and musculoskeletal disorders. However, physical activity is known not only to have positive effects. In fact, the achievement of a good performance requires a very high oxygen consumption, which leads to the formation of oxygen free radicals, responsible for premature cell aging and diseases such as heart failure and muscle injury. In this scenario, a primary role is played by antioxidants, in particular by natural antioxidants that can be taken through the diet. Natural antioxidants are molecules capable of counteracting oxygen free radicals without causing cellular cytotoxicity. In recent years, therefore, research has conducted numerous studies on the identification of natural micronutrients, in order to prevent or mitigate oxidative stress induced by physical activity by helping to support conventional drug therapies against heart failure and muscle damage. The aim of this review is to have an overview of how controlled physical activity and a diet rich in antioxidants can represent a "natural cure" to prevent imbalances caused by free oxygen radicals in diseases such as heart failure and muscle damage. In particular, we will focus on sulfur-containing compounds that have the ability to protect the body from oxidative stress. We will mainly focus on six natural antioxidants: glutathione, taurine, lipoic acid, sulforaphane, garlic and methylsulfonylmethane.
Asunto(s)
Dieta , Insuficiencia Cardíaca , Compuestos de Sulfhidrilo , Antioxidantes , Insuficiencia Cardíaca/etiología , Insuficiencia Cardíaca/prevención & control , Humanos , Estrés Oxidativo , Especies Reactivas de Oxígeno , Compuestos de Sulfhidrilo/toxicidadRESUMEN
Xanthatin, a natural sesquiterpene lactone, occurs as one of the major constituents of Xanthium plants (Compositae) and exhibits many important biological properties. To discover natural products-based pesticides, forty-nine Michael-type thiol/amino adducts of xanthatin were synthesized and characterized, while their pesticidal activities were investigated. Among them, compounds 2c, 2h, 2i, and 2t exhibited more potent antifungal activity against Botrytis cinerea (IC50 = 0.96, 0.38, 6.33, and 7.21 µg/mL, respectively) than xanthatin and the two commercial fungicides. Compounds 2t and 2u displayed broad-spectrum and excellent antifungal effects against all tested phytopathogenic fungi, while their IC50 values ranged from 7.21 to 75.88 µg/mL. Compounds 2a, 2f, 2l, 2m, 2v, 7c, 7e, 7h, 7i, and 7j showed moderate larvicidal activity against Plutella xylostella Linnaeus. Furthermore, compounds 2b, 7g, and 7h demonstrated significant ovicidal activity against P. xylostella with the LC50 values of 14.04, 10.00, and 11.95 mg/L, respectively. These findings suggest that thiol/amino appended in the C-13 position of xanthatin may improve antifungal and ovicidal activities for the derivatives. It was also noticed that the exocyclic double bond of xanthatin is crucial for its larvicidal activity. This work also provides some important hints for further design, synthesis, and structural modification of the xanthanolides sesquiterpene lactones toward development of the new environmentally friendly pesticides for sustainable agricultural production.
Asunto(s)
Botrytis/efectos de los fármacos , Fungicidas Industriales/toxicidad , Furanos/toxicidad , Enfermedades de las Plantas/microbiología , Xanthium/química , Aminación , Fungicidas Industriales/síntesis química , Fungicidas Industriales/química , Furanos/síntesis química , Furanos/química , Modelos Moleculares , Enfermedades de las Plantas/prevención & control , Compuestos de Sulfhidrilo/síntesis química , Compuestos de Sulfhidrilo/química , Compuestos de Sulfhidrilo/toxicidadRESUMEN
The protective effect of taurine against inflammation, apoptosis and oxidative stress in traumatic brain injury was investigated in the present study. Taurine is a nonproteogenic and essential amino acid in animals. It plays a critical nutritional role in brain cell growth, differentiation, and development. Taurine is involved in regeneration and neuroprotection in the injured nervous system, and is an effective antioxidant against lead, cadmium, and exerciseinduced oxidative stress. Astrocytes and neuron cells were cocultured and cells were treated with different concentrations of taurine (100, 200 and 300 mg/l) for 72 h, and the levels of reactive oxygen species, malondialdehyde, reduced glutathione, glutathione peroxidase, superoxide dismutase, catalase, acetylcholinesterase, tumor necrosis factorα, interleukin6, caspase3, p53, Bcell lymphoma 2 and Bcl2associated X protein were determined. These inflammatory, apoptotic, and oxidative stress markers were substantially increased in injured cells, and returned to normal levels following taurine supplementation. Thus, taurine supplementation may be effective against oxidative stress, apoptosis, and inflammation in injured brain cells.
Asunto(s)
Antioxidantes/farmacología , Encéfalo/efectos de los fármacos , Inflamación/tratamiento farmacológico , Taurina/farmacología , Acetilcolinesterasa/metabolismo , Animales , Antioxidantes/química , Apoptosis/efectos de los fármacos , Astrocitos/química , Astrocitos/efectos de los fármacos , Encéfalo/patología , Catalasa/metabolismo , Técnicas de Cocultivo , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Humanos , Inflamación/inducido químicamente , Inflamación/patología , Malondialdehído/metabolismo , Neuronas/química , Neuronas/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Piranos/toxicidad , Ratas , Especies Reactivas de Oxígeno/metabolismo , Compuestos de Sulfhidrilo/toxicidad , Superóxido Dismutasa/metabolismo , Taurina/química , Proteína X Asociada a bcl-2/genéticaRESUMEN
Synthesis, in vitro cytotoxic activity, and interaction with tubulin of oxidized, isomeric 1-(5-alkoxybenzo[d][1,3]oxathiol-6-yl)-3-phenylprop-2-en-1-ones and 1-(6-alkoxybenzo[d][1,3]oxathiol-5-yl)-3-phenylprop-2-en-1-ones are described. Most of the compounds demonstrated cytotoxic activity at submicromolar concentrations. It was found that oxidation of sulfur atom of the oxathiole-fused chalcones strongly influenced activity of the parent compounds, and that depending on relative position of the sulfur atom in the molecule, the activity was either increased or diminished. For isomers with sulfur atom para to the chalcone carbonyl group, oxidation led to increase in activity, while for isomers with sulfur atom meta to the carbonyl the activity dropped down. It was demonstrated that the compounds interact with tubulin at the colchicine binding site, and the interaction was evaluated using molecular modeling. It was concluded that the observed profound influence of oxidation of the sulfur atom on cytotoxic activity cannot be solely related to interaction of the compounds with tubulin.
Asunto(s)
Supervivencia Celular/efectos de los fármacos , Chalconas/síntesis química , Chalconas/toxicidad , Compuestos de Sulfhidrilo/síntesis química , Compuestos de Sulfhidrilo/toxicidad , Azufre/química , Línea Celular Tumoral , Chalconas/química , Citotoxinas/síntesis química , Citotoxinas/química , Citotoxinas/toxicidad , Evaluación Preclínica de Medicamentos , Células HeLa , Humanos , Concentración 50 Inhibidora , Estructura Molecular , Oxidación-Reducción , Compuestos de Sulfhidrilo/químicaRESUMEN
6-Oxo and 6-thio analogs of purine were prepared based on the initial activity screening of a small, diverse purine library against Mycobacterium tuberculosis (Mtb). Certain 6-oxo and 6-thio-substituted purine analogs described herein showed moderate to good inhibitory activity. N(9)-substitution apparently enhances the anti-mycobacterial activity in the purine series described herein. Several 2-amino and 2-chloro purine analogs were also synthesized that showed moderate inhibitory activity against Mtb.
Asunto(s)
Antituberculosos/química , Antituberculosos/uso terapéutico , Mycobacterium tuberculosis/efectos de los fármacos , Purinas/química , Purinas/uso terapéutico , Tuberculosis/tratamiento farmacológico , Animales , Antituberculosos/farmacología , Antituberculosos/toxicidad , Supervivencia Celular/efectos de los fármacos , Chlorocebus aethiops , Humanos , Ratones , Pruebas de Sensibilidad Microbiana , Purinas/farmacología , Purinas/toxicidad , Compuestos de Sulfhidrilo/química , Compuestos de Sulfhidrilo/farmacología , Compuestos de Sulfhidrilo/uso terapéutico , Compuestos de Sulfhidrilo/toxicidad , Células VeroRESUMEN
No pro-apoptotic effect of dinitrosyl iron complexes (DNIC) with glutathione, cysteine or thiosulfate was established after incubation of HeLa cells in Eagle's medium. However, DNIC with thiosulfate manifested pro-apoptotic activity during incubation of HeLa cells in Versene's solution supplemented with ethylene diamine tetraacetate (EDTA) known to induce the decomposition of these DNIC. The water-soluble о-phenanthroline derivative bathophenanthroline disulfonate (BPDS) had a similar effect on DNIC with glutathione during incubation of HeLa cells in Eagle's medium. It was assumed that EDTA- or BPDS-induced pro-apoptotic effect of DNIC with thiosulfate or glutathione is coupled with the ability of decomposing DNIC to initiate S-nitrosylation of proteins localized on the surface of HeLa cells. Presumably, the pro-apoptotic effect of S-nitrosoglutathione (GS-NO) on HeLa cells preincubated in Eagle's medium is mediated by the same mechanism, although the pro-apoptotic effect based on the ability of GS-NO to initiate the release of significant amounts of NO and its oxidation to cytotoxic peroxynitrite in a reaction with superoxide should not be ruled out either. No apoptotic activity was found in the presence of bivalent iron and glutathione favoring the conversion of GS-NO into DNIC with glutathione. It is suggested that interaction of HeLa cells with intact DNIC with glutathione or thiosulfate results in the formation of DNIC bound to cell surface proteins.
Asunto(s)
Apoptosis/efectos de los fármacos , Hierro/toxicidad , Donantes de Óxido Nítrico/toxicidad , Óxido Nítrico/metabolismo , Óxidos de Nitrógeno/toxicidad , Compuestos de Sulfhidrilo/toxicidad , Quelantes/metabolismo , Quelantes/toxicidad , Cisteína/metabolismo , Ácido Edético/metabolismo , Ácido Edético/toxicidad , Glutatión/metabolismo , Células HeLa , Humanos , Hierro/metabolismo , Ligandos , Óxido Nítrico/toxicidad , Donantes de Óxido Nítrico/metabolismo , Óxidos de Nitrógeno/metabolismo , Oxidantes/metabolismo , Oxidantes/toxicidad , Oxidación-Reducción , Fenantrolinas/metabolismo , Fenantrolinas/toxicidad , S-Nitrosoglutatión/metabolismo , S-Nitrosoglutatión/toxicidad , Compuestos de Sulfhidrilo/metabolismo , Tiosulfatos/metabolismo , Tiosulfatos/toxicidadRESUMEN
Organosulphur compounds from garlic, especially diallyl disulphide (DADS) at non-toxic concentrations, affected production and secretion of some matrix metalloproteinases (MMPs) and of tissue inhibitor of metalloproteinase-1 (TIMP-1), one of their inhibitors, by human umbilical vein endothelial cells. Addition of DADS to the culture medium resulted in a concentration-dependent reduction of secreted MMP-2 protein and activity as well as TIMP-1 protein. In the presence of inducers (phorbol 12-myristate 13-acetate, forskolin and tumor necrosis factor alpha) addition of DADS caused a distinct concentration-dependent decrease of MMP-9 and TIMP-1 secretion, while not affecting MMP-9 mRNA levels. Intracellular protein levels remained low and were not affected. Other organosulphur compounds like allyl mercaptan and S-allylcysteine showed no or less clear effects on MMP-secretion or TIMP-1-secretion. These results suggest that DADS may mediate some of the biological effects ascribed to garlic preparations through affecting MMP-TIMP balance.
Asunto(s)
Compuestos Alílicos/toxicidad , Cisteína/análogos & derivados , Cisteína/toxicidad , Disulfuros/toxicidad , Endotelio Vascular/enzimología , Ajo , Metaloproteinasas de la Matriz/metabolismo , Compuestos de Sulfhidrilo/toxicidad , Inhibidor Tisular de Metaloproteinasa-1/metabolismo , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Humanos , Venas UmbilicalesRESUMEN
Chelation therapy is the basis for the treatment of metal poisoning. A number of chelating agents have been widely used since the 1950s. Since these agents can be potentially given to a metal-intoxicated pregnant woman, their intrinsic developmental toxicities are a matter of concern. While the embryo/fetal toxic effects of some chelators have been reported to occur at doses higher than those currently given in the medical treatment of metal poisoning, according to experimental data the potential use of other metal antidotes is controversial. In those cases, the benefits and risks of usage should be carefully weighed. The developmental toxicity of known chelators of clinical interest is presented here. Chelating agents were divided according to the following structurally related categories: polyaminocarboxylic acids, chelators with vicinal -SH groups, beta-mercapto-alpha-aminoacids, hydroxamic acids, ortho-hydroxycarboxylic acids, and miscellaneous agents. Since it has been demonstrated that the teratogenic potential of most chelators is, at least in part, due to induced trace element deficiencies, the advisability of mineral supplements during chelation treatment is also discussed.
Asunto(s)
Anomalías Inducidas por Medicamentos/etiología , Quelantes/toxicidad , Aminoácidos/toxicidad , Animales , Relación Dosis-Respuesta a Droga , Desarrollo Embrionario y Fetal/efectos de los fármacos , Femenino , Metales/envenenamiento , Fenoles/toxicidad , Embarazo , Compuestos de Sulfhidrilo/toxicidadRESUMEN
Delayed-type skin allergic (DTA) reactions induced by various sulfhydryl compounds were investigated. Sulfhydryl compounds investigated were bucillamine and D-penicillamine: antirheumatic agents, SA981: an intramolecular cyclic disulfide of bucillamine, tiopronin: a hepatoprotective and potent antirheumatic agent and captopril: antihypertensive and potent antirheumatic agent. Guinea pigs were sensitized on day 1 by subcutaneous injection and day 7 by subcutaneous and intramuscular injection of emulsions of these compounds (3 mg/animal) with Freund's complete adjuvant. Two weeks after the second sensitization, 0.3 mg/animal of each compound was intradermally challenged and the 24 hr DTA reaction was evaluated. A large number of sulfhydril compounds showed positive in the DTA reactions and the intensity of these reactions had a good correlation with the frequency of the skin rashes referred to by the adverse effects in clinical reports of anti-rheumatic drugs. It is suggested that guinea pig DTA reaction may provide a good screening way to find new sulfhydryl or disulfide compounds with low incidence of skin rash. A novel sulfhydryl compound N-(2,2-dimethyl-3-mercaptopropionyl)-L-cysteine (SA3441) and its intramolecular cyclic disulfide (4R)-hexahydro-7,7-dimethyl-6-oxo-1,2,5-dithiazocine-4-carboxylic acid (SA3443), which show no DTA activity in guinea pigs, were found using the above-mentioned DTA reaction.
Asunto(s)
Antiinflamatorios no Esteroideos/toxicidad , Dermatitis Alérgica por Contacto/etiología , Hipersensibilidad a las Drogas/etiología , Hipersensibilidad Tardía/inducido químicamente , Compuestos de Sulfhidrilo/toxicidad , Animales , Antiinflamatorios no Esteroideos/química , Antiinflamatorios no Esteroideos/inmunología , Artritis Reumatoide/tratamiento farmacológico , Cisteína/análogos & derivados , Cisteína/química , Cisteína/inmunología , Cisteína/toxicidad , Cobayas , Humanos , Inmunoquímica , Masculino , Especificidad de la Especie , Relación Estructura-Actividad , Compuestos de Sulfhidrilo/química , Compuestos de Sulfhidrilo/inmunologíaRESUMEN
In the 12-day-old rat cochlea, the synthesis of inositol phosphates (IPs) can be activated via M3 cholinoceptors. This stimulation is blocked by ototoxins (mercury, ethacrynate, cisplatin, neomycin), drugs with side effects that lead to damage of hair cells and strial cells. As these toxic effects can be reversed in vivo by thiol molecules, we investigated whether modifications of thiol compounds could be involved in ototoxin-induced inhibition of the IP turnover in the cochlea. For this purpose, we assessed whether the sulphhydryl-modifying reagents N-ethylmaleimide and cadmium modify the carbachol-stimulated formation of IPs in the 12-day-old rat cochlea. Both molecules inhibit the carbachol effect on a dose-dependent way without altering the basal metabolism of IPs. As cadmium may block some calcium channels, the effect of verapamil, another calcium channel antagonist, was tested. Verapamil (1-50 microM) does not alter carbachol-evoked IP formation, suggesting that the inhibitory effect of cadmium is not due to a calcium influx block. Binding experiments with the muscarinic ligand quinuclidinyl benzylate (QNB) showed that the sulphhydryl-modifying reagents do not displace QNB from binding sites. Combining ototoxins and reagents shows that N-ethylmaleimide acts synergistically with all ototoxins but ethacrynate while cadmium does so only with mercury. Both N-ethylmaleimide and cadmium have additive effects with ethacrynate. As a supplement, disulphide bond-modifying agents do not alter the carbachol-enhanced metabolism of IPs. These results suggest that molecules having thiol-modifying properties inhibit the carbachol-induced turnover of IPs without acting at the muscarinic sites.(ABSTRACT TRUNCATED AT 250 WORDS)