RESUMEN
Previously we demonstrated, in rats, that treatment with growth hormone (GH) and rehabilitation, carried out immediately after a motor cortical ablation, significantly improved the motor affectation produced by the lesion and induced the re-expression of nestin in the contralateral motor cortex. Here we analyze cortical proliferation after ablation of the frontal motor cortex and investigate the re-expression of nestin in the contralateral motor cortex and the role of the striatum and thalamus in motor recovery. The rats were subjected to ablation of the frontal motor cortex in the dominant hemisphere or sham-operated and immediately treated with GH or the vehicle (V), for five days. At 1 dpi (days post-injury), all rats received daily injections (for four days) of bromodeoxyuridine and five rats were sacrificed at 5 dpi. The other 15 rats (n = 5/group) underwent rehabilitation and were sacrificed at 25 dpi. GH induced the greatest number of proliferating cells in the perilesional cortex. GH and rehabilitation produced the functional recovery of the motor lesion and increased the expression of nestin in the striatum. In the thalamic ventral nucleus ipsilateral to the lesion, cells positive for nestin and actin were detected, but this was independent on GH. Our data suggest that GH-induced striatal nestin is involved in motor recovery.
Asunto(s)
Actinas/metabolismo , Lesiones Encefálicas/tratamiento farmacológico , Cuerpo Estriado/metabolismo , Hormona del Crecimiento/uso terapéutico , Nestina/metabolismo , Tálamo/metabolismo , Animales , Lesiones Encefálicas/rehabilitación , Proliferación Celular , Cuerpo Estriado/patología , Expresión Génica , Masculino , Corteza Motora/lesiones , Corteza Motora/patología , Ratas , Recuperación de la Función , Tálamo/patologíaRESUMEN
The organization of brain areas in functionally connected networks, their dynamic changes, and perturbations in disease states are subject of extensive investigations. Research on functional networks in humans predominantly uses functional magnetic resonance imaging (fMRI). However, adopting fMRI and other functional imaging methods to mice, the most widely used model to study brain physiology and disease, poses major technical challenges and faces important limitations. Hence, there is great demand for alternative imaging modalities for network characterization. Here, we present a refined protocol for in vivo widefield calcium imaging of both cerebral hemispheres in mice expressing a calcium sensor in excitatory neurons. We implemented a stringent protocol for minimizing anesthesia and excluding movement artifacts which both imposed problems in previous approaches. We further adopted a method for unbiased identification of functional cortical areas using independent component analysis (ICA) on resting-state imaging data. Biological relevance of identified components was confirmed using stimulus-dependent cortical activation. To explore this novel approach in a model of focal brain injury, we induced photothrombotic lesions of the motor cortex, determined changes in inter- and intrahemispheric connectivity at multiple time points up to 56 days post-stroke and correlated them with behavioral deficits. We observed a severe loss in interhemispheric connectivity after stroke, which was partially restored in the chronic phase and associated with corresponding behavioral motor deficits. Taken together, we present an improved widefield calcium imaging tool accounting for anesthesia and movement artifacts, adopting an advanced analysis pipeline based on human fMRI algorithms and with superior sensitivity to recovery mechanisms in mouse models compared to behavioral tests. This tool will enable new studies on interhemispheric connectivity in murine models with comparability to human imaging studies for a wide spectrum of neuroscience applications in health and disease.
Asunto(s)
Calcio , Corteza Cerebral/fisiología , Conectoma/métodos , Red Nerviosa/fisiología , Neuroimagen/métodos , Imagen Óptica/métodos , Prosencéfalo/fisiología , Accidente Cerebrovascular/fisiopatología , Animales , Corteza Cerebral/diagnóstico por imagen , Corteza Cerebral/fisiopatología , Modelos Animales de Enfermedad , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Corteza Motora/lesiones , Corteza Motora/fisiopatología , Red Nerviosa/diagnóstico por imagen , Red Nerviosa/fisiopatología , Prosencéfalo/diagnóstico por imagen , Prosencéfalo/fisiopatología , Accidente Cerebrovascular/diagnóstico por imagenRESUMEN
Traumatic brain injury (TBI) has been proposed as a risk factor for neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). To determine whether TBI might trigger or exacerbate ALS-relevant pathology, we delivered a mild stab-wound injury to the motor cortex of three different ALS mouse models expressing mutations in SOD1, TDP-43 or FUS and scrutinized the effects on the formation of phospho-TDP-43 (pTDP-43) cytoplasmic granules. Stab-injury induced the formation of cytoplasmic TDP-43 granules in wt animals, peaking at 3dpi; a much larger response was seen in mutant TDP-43 mice, whose response peaked at 7dpi. The pTDP-43 granules did not colocalize with the stress markers TIAR-1 and FUS but colocalized with FMRP (35%) and with p62 (65%), suggesting their involvement in transport granules and their clearance by autophagy. A similar, albeit smaller effect, was seen in mutant FUS mice. In the SOD1G93A mouse model, neither increase in pTDP-43 granules nor in SOD1 aggregates were detected. In all cases, pTDP-43 granules were cleared and the number of pTDP-43-positive neurons returned to baseline by 40dpi. Neither injury-related neuronal loss nor motor performance or survival was significantly different in transgenic mice receiving injury vs sham mice. Thus, trauma can trigger ALS-related TDP-43 pathology, the extent of which is modulated by ALS-related mutations. However, the pathological findings prove reversible and do not affect disease progression and neuronal vulnerability.
Asunto(s)
Lesiones Traumáticas del Encéfalo/patología , Proteínas de Unión al ADN/metabolismo , Corteza Motora/patología , Esclerosis Amiotrófica Lateral/patología , Animales , Autofagia/genética , Conducta Animal , Lesiones Traumáticas del Encéfalo/psicología , Gránulos Citoplasmáticos/patología , Proteínas de Unión al ADN/genética , Modelos Animales de Enfermedad , Inmunohistoquímica , Ratones , Ratones Transgénicos , Corteza Motora/lesiones , Neuronas Motoras/patología , Proteína FUS de Unión a ARN/genética , Proteína FUS de Unión a ARN/metabolismo , Superóxido Dismutasa-1/genética , Superóxido Dismutasa-1/metabolismoRESUMEN
BACKGROUND: Motor impairments are among the major complications that develop after cortical damage caused by either stroke or traumatic brain injury. Motor cortex stimulation (MCS) can improve motor functions in animal models of stroke by inducing neuroplasticity. OBJECTIVE: In the current study, the therapeutic effect of chronic MCS was assessed in a rat model of severe cortical damage. METHODS: A controlled cortical impact (CCI) was applied to the forelimb area of the motor cortex followed by implantation of a flat electrode covering the lesioned area. Forelimb function was assessed using the Montoya staircase test and the cylinder test before and after a period of chronic MCS. Furthermore, the effect of MCS on tissue metabolism and lesion size was measured using [18F]-fluorodesoxyglucose (FDG) µPET scanning. RESULTS: CCI caused a considerable lesion at the level of the motor cortex and dorsal striatum together with a long-lasting behavioral phenotype of forelimb impairment. However, MCS applied to the CCI lesion did not lead to any improvement in limb functioning when compared to non-stimulated control rats. Also, MCS neither changed lesion size nor distribution of FDG. CONCLUSION: The use of MCS as a standalone treatment did not improve motor impairments in a rat model of severe cortical damage using our specific treatment modalities.
Asunto(s)
Lesiones Encefálicas/terapia , Terapia por Estimulación Eléctrica/métodos , Miembro Anterior/fisiopatología , Corteza Motora/lesiones , Corteza Motora/fisiopatología , Recuperación de la Función/fisiología , Animales , Conducta Animal/fisiología , Lesiones Encefálicas/fisiopatología , Modelos Animales de Enfermedad , RatasRESUMEN
BACKGROUND: Electrical and magnetic brain stimulation can improve motor function following stroke in humans, rats, and nonhuman primates, especially when paired with rehabilitative training (RT). Previously, we found in rodent stroke models that epidural electrical cortical stimulation (CS) of the ipsilesional motor cortex (MC) combined with motor RT enhances motor function and motor cortical plasticity. It was unknown whether CS following experimental traumatic brain injury (TBI) would have similar effects. OBJECTIVE: To test the effects of CS combined with motor training after moderate/severe TBI on behavioral outcome and motor cortical organization. METHODS: Following unilateral controlled cortical impact (CCI) over the caudal forelimb area of the MC in adult male rats, forelimb reach training was administered daily for 9 weeks concurrently with subthreshold, 100-Hz monopolar CS or no-stimulation control procedures. The rate and magnitude of behavioral improvements and changes in forelimb movement representations in the injured MC as revealed by intracortical microstimulation were measured. RESULTS: CCI resulted in severe motor impairments persisting throughout the 9 weeks of training in both groups, but CS-treated animals had significantly greater behavioral improvements. CS also increased wrist motor cortical representation, one of the main movements used in the training task, when compared with RT alone. However, the overall recovery level was modest, leaving animals still extremely impaired. CONCLUSIONS: These data suggest that CS may be useful for improving rehabilitation efficacy after TBI but also raise the possibility that the CS parameters that are highly effective following stroke are suboptimal after moderate/severe TBI.
Asunto(s)
Lesiones Traumáticas del Encéfalo/terapia , Terapia por Estimulación Eléctrica/métodos , Corteza Motora/lesiones , Corteza Motora/fisiopatología , Recuperación de la Función/fisiología , Animales , Conducta Animal/fisiología , Modelos Animales de Enfermedad , Terapia por Estimulación Eléctrica/normas , Masculino , Plasticidad Neuronal , Ratas , Ratas Long-EvansRESUMEN
Proline-rich tyrosine kinase 2 (Pyk2) is a calcium-dependent, non-receptor protein-tyrosine kinase of the focal adhesion kinase (FAK) family. Pyk2 is enriched in the brain, especially the forebrain. Pyk2 is highly expressed in neurons but is also present in astrocytes, where its role is not known. We used Pyk2 knockout mice (Pyk2(-/-) ) developed in our laboratory to investigate the function of Pyk2 in astrocytes. Morphology and basic properties of astrocytes in vivo and in culture were not altered in the absence of Pyk2. However, following stab lesions in the motor cortex, astrocytes-mediated wound filling was slower in Pyk2(-/-) than in wild-type littermates. In an in vitro wound healing model, Pyk2(-/-) astrocytes migrated slower than Pyk2(+/+) astrocytes. The role of Pyk2 in actin dynamics was investigated by treating astrocytic cultures with the actin-depolymerizing drug latrunculin B. Actin filaments re-polymerization after latrunculin B treatment was delayed in Pyk2(-/-) astrocytes as compared with wild-type astrocytes. We mimicked wound-induced activation by treating astrocytes in culture with tumor-necrosis factor alpha (TNFα), which increased Pyk2 phosphorylation at Tyr402. TNFα increased PKC activity, and Rac1 phosphorylation at Ser71 similarly in wild-type and Pyk2-deficient astrocytes. Conversely, we found that gelsolin, an actin-capping protein known to interact with Pyk2 in other cell types, was less enriched at the leading edge of migrating Pyk2(-/-) astrocytes, suggesting that its lack of recruitment mediated in part the effects of the mutation. This work shows the critical role of Pyk2 in astrocytes migration during wound healing.
Asunto(s)
Astrocitos/enzimología , Lesiones Encefálicas/enzimología , Movimiento Celular/fisiología , Quinasa 2 de Adhesión Focal/metabolismo , Corteza Motora/enzimología , Corteza Motora/lesiones , Actinas/metabolismo , Animales , Astrocitos/patología , Lesiones Encefálicas/patología , Células Cultivadas , Modelos Animales de Enfermedad , Quinasa 2 de Adhesión Focal/genética , Gelsolina/metabolismo , Ratones Noqueados , Corteza Motora/patología , Neuropéptidos/metabolismo , Fosforilación , Proteína Quinasa C/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo , Cicatrización de Heridas/fisiología , Proteína de Unión al GTP rac1/metabolismoRESUMEN
Ischemic stroke insults may lead to chronic functional limitations that adversely affect patient movements. Partial motor recovery is thought to be sustained by neuronal plasticity, particularly in areas close to the lesion site. It is still unknown if treatments acting exclusively on cortical plasticity of perilesional areas could result in behavioral amelioration. We tested whether enhancing plasticity in the ipsilesional cortex using local injections of chondroitinase ABC (ChABC) could promote recovery of skilled motor function in a focal cortical ischemia of forelimb motor cortex in rats. Using the skilled reaching test, we found that acute and delayed ChABC treatment induced recovery of impaired motor skills in treated rats. vGLUT1, vGLUT2, and vGAT staining indicated that functional recovery after acute ChABC treatment was associated with local plastic modification of the excitatory cortical circuitry positive for VGLUT2. ChABC effects on vGLUT2 staining were present only in rats undergoing behavioral training. Thus, the combination of treatments targeting the CSPG component of the extracellular matrix in perilesional areas and rehabilitation could be sufficient to enhance functional recovery from a focal stroke.
Asunto(s)
Isquemia Encefálica/terapia , Condroitina ABC Liasa/uso terapéutico , Terapia por Ejercicio , Recuperación de la Función/efectos de los fármacos , Accidente Cerebrovascular/terapia , Animales , Isquemia Encefálica/tratamiento farmacológico , Condroitina ABC Liasa/farmacología , Terapia Combinada , Corteza Motora/efectos de los fármacos , Corteza Motora/lesiones , Corteza Motora/patología , Plasticidad Neuronal/efectos de los fármacos , Ratas , Ratas Long-Evans , Accidente Cerebrovascular/tratamiento farmacológico , Sinapsis/efectos de los fármacos , Sinapsis/metabolismoRESUMEN
Traumatic brain injury (TBI) results in enduring functional deficits. Strategies aimed at promoting plasticity within the injured brain may aid in enhancing functional outcome. We have previously shown that spontaneous pericontusional axon sprouting occurs within 7-14 days after controlled cortical impact injury in the adult rat, but ultimately fails due to an increasingly growth-inhibitory environment. We therefore sought to determine whether acute infusion of chondroitinase ABC into the site of the cortical contusion, to further reduce pericontusional growth-inhibitory chondroitin sulfate proteoglycans (CSPGs), would enhance and prolong the sprouting response. We also wanted to determine if chondroitinase-enhanced sprouting would ameliorate the behavioral deficits in forelimb function that occur in this model. Acute chondroitinase infusion decreased intact CSPGs and significantly increased pericontusional cortical grey and white matter growth-associated protein 43 (GAP43)-positive axon sprouting at 7 days post-injury. A return of intact CSPGs at later time points likely contributed to the absence of persistently increased levels of axon sprouting by 14-21 days post-injury. There was no overall benefit on forelimb function during the time of maximal sprouting or at any subsequent times in three of four behavioral outcome measures. However, there was a chondroitinase-induced improvement in recovery from unskilled limb use deficits on the staircase forelimb reaching test toward sham-injured values at 28 days, which was not achieved by the vehicle-treated rats, indicating that there is some minor functional benefit of the increased sprouting induced by chondroitinase treatment. The current results, together with data from spinal cord injury models after chondroitinase intervention, suggest that a combinatorial approach with the addition of neurotrophins and rehabilitation would result in more robust axon sprouting and consequently improve behavioral outcome.
Asunto(s)
Axones/efectos de los fármacos , Conducta Animal/fisiología , Lesiones Encefálicas/tratamiento farmacológico , Lesiones Encefálicas/patología , Condroitina ABC Liasa/farmacología , Animales , Atrofia , Lesiones Encefálicas/psicología , Recuento de Células , Proteoglicanos Tipo Condroitín Sulfato/metabolismo , Proteína GAP-43/metabolismo , Procesamiento de Imagen Asistido por Computador , Inmunohistoquímica , Masculino , Corteza Motora/lesiones , Corteza Motora/patología , Procedimientos Neuroquirúrgicos , Desempeño Psicomotor/fisiología , Ratas , Ratas Sprague-Dawley , Corteza Somatosensorial/lesiones , Corteza Somatosensorial/patología , Caminata/fisiologíaRESUMEN
When neurons within the motor cortex are fatally injured, their axons, many of which project into the spinal cord, undergo wallerian degeneration. Pathological processes occurring downstream of the cortical damage have not been extensively studied. We created a focal forelimb motor cortex injury in rats and found that axons from cell bodies located in the hindlimb motor cortex (spared by the cortical injury) become secondarily damaged in the spinal cord. To assess axonal degeneration in the spinal cord, we quantified silver staining in the corticospinal tract (CST) at 1 week and 4 weeks after the injury. We found a significant increase in silver deposition at the thoracic spinal cord level at 4 weeks compared to 1 week post-injury. At both time points, no degenerating neurons could be found in the hindlimb motor cortex. In a separate experiment, we showed that direct injury of neurons within the hindlimb motor cortex caused marked silver deposition in the thoracic CST at 1 week post-injury, and declined thereafter. Therefore, delayed axonal degeneration in the thoracic spinal cord after a focal forelimb motor cortex injury is indicative of secondary damage at the spinal cord level. Furthermore, immunolabeling of spinal cord sections showed that a local inflammatory response dominated by partially activated Iba-1-positive microglia is mounted in the CST, a viable mechanism to cause the observed secondary degeneration of fibers. In conclusion, we demonstrate that following motor cortex injury, wallerian degeneration of axons in the spinal cord leads to secondary damage, which is likely mediated by inflammatory processes.
Asunto(s)
Corteza Motora/lesiones , Corteza Motora/patología , Médula Espinal/patología , Animales , Benzoxazinas , Proteínas de Unión al Calcio/biosíntesis , Proteínas de Unión al Calcio/genética , Circulación Cerebrovascular/fisiología , Colorantes , Vías Eferentes/lesiones , Fluoresceínas , Colorantes Fluorescentes , Miembro Anterior/inervación , Miembro Posterior/inervación , Inmunohistoquímica , Masculino , Proteínas de Microfilamentos , Microglía/patología , Corteza Motora/irrigación sanguínea , Degeneración Nerviosa/patología , Fibras Nerviosas/patología , Compuestos Orgánicos , Oxazinas , Perfusión , Ratas , Ratas Sprague-Dawley , Tinción con Nitrato de PlataRESUMEN
Axonal injury is a major hallmark of traumatic brain injury (TBI), and it seems likely that therapies directed toward enhancing axon repair could potentially improve functional outcomes. One potential target is chondroitin sulfate proteoglycans (CSPGs), which are major axon growth inhibitory molecules that are generally, but not always, up-regulated after central nervous system injury. The current study was designed to determine temporal changes in cerebral cortical mRNA or protein expression levels of CSPGs and to determine their regional localization and cellular association by using immunohistochemistry in a controlled cortical impact model of TBI. The results showed significant increases in versican mRNA at 4 and 14 days after TBI but no change in neurocan, aggrecan, or phosphacan. Semiquantitative Western blot (WB) analysis of cortical CSPG protein expression revealed a significant ipsilateral decrease of all CSPGs at 1 day after TBI. Lower CSPG protein levels were sustained until at least 14 days, after which the levels began to normalize. Immunohistochemistry data confirm previous reports of regional increases in CSPG proteins after CNS injury, seen primarily within the developing glial scar after TBI, but also corroborate the WB data by revealing wide areas of pericontusional tissue that are deficient in both extracellular and perineuronal net-associated CSPGs. Given the evidence that CSPGs are largely inhibitory to axonal growth, we interpret these data to indicate a potential for regional spontaneous plasticity after TBI. If this were the case, the gradual normalization of CSPG proteins over time postinjury would suggest that this may be temporally as well as regionally limited.
Asunto(s)
Lesiones Encefálicas/metabolismo , Proteoglicanos Tipo Condroitín Sulfato/biosíntesis , Regulación de la Expresión Génica , Proteínas del Tejido Nervioso/biosíntesis , Animales , Lesiones Encefálicas/genética , Lesiones Encefálicas/patología , Proteoglicanos Tipo Condroitín Sulfato/genética , Cicatriz/etiología , Cicatriz/genética , Cicatriz/metabolismo , Cicatriz/patología , Gliosis/etiología , Gliosis/genética , Gliosis/metabolismo , Gliosis/patología , Masculino , Corteza Motora/lesiones , Corteza Motora/metabolismo , Proteínas del Tejido Nervioso/genética , Plasticidad Neuronal , ARN Mensajero/biosíntesis , ARN Mensajero/genética , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Corteza Somatosensorial/lesiones , Corteza Somatosensorial/metabolismo , Factores de Tiempo , Versicanos/biosíntesis , Versicanos/genética , Cicatrización de HeridasRESUMEN
Functional reorganization of brain cortical areas occurs following stroke in humans, and many instances of this plasticity are associated with recovery of function. Rodent studies have shown that following a cortical stroke, neurons in uninjured areas of the brain are capable of sprouting new axons into areas previously innervated by injured cortex. The pattern and extent of structural plasticity depend on the species, experimental model, and lesion localization. In this study, we examined the pattern of axon sprouting in spinal cord after a localized lesion which selectively targeted the primary motor cortex in adult mice. We subjected mice to a stereotaxic-guided photothrombotic stroke of the left motor cortex, followed 2 weeks later by an injection of the neuronal tracer biotinylated dextran amine (BDA) into the uninjured right motor cortex. BDA-positive axons originating from the uninjured motor cortex were increased in the gray matter of the right cervical spinal cord in stroke mice, compared to sham control mice. These results show that axon sprouting can occur in the spinal cord of adult wild-type mice after a localized stroke in motor cortex.
Asunto(s)
Axones/fisiología , Corteza Motora/patología , Médula Espinal/patología , Accidente Cerebrovascular/patología , Accidente Cerebrovascular/fisiopatología , Animales , Biotina/análogos & derivados , Biotina/metabolismo , Dextranos/metabolismo , Modelos Animales de Enfermedad , Ratones , Corteza Motora/lesiones , Vías Nerviosas/patologíaRESUMEN
After motor cortex damage, the unaffected homotopic cortex shows changes in motor output. The present experiments were designed to clarify the nature of these interhemispheric effects. We investigate the vibrissa motor cortex (VMC) output after activity suppression of the homotopic area in adult rats. Comparison was made of VMC output after lidocaine inactivation (L-group) or quinolinic acid lesion (Q-group) of the homotopic cortex. In the Q-group, VMC mapping was performed 3 days (Q3Ds group), 2 weeks (Q2Ws group) and 4 weeks (Q4Ws group) after cortical lesion. In each animal, VMC output was assessed by mapping movements induced by intracortical microstimulation (ICMS) in both hemispheres (hemisphere ipsilateral and contralateral to injections). Findings demonstrated that, in the L-group, the size of vibrissal representation was 39.5% smaller and thresholds required to evoke vibrissa movement were 46.3% higher than those in the Control group. There was an increase in the percentage of ineffective sites within the medial part of the VMC and an increase in the percentage of forelimb sites within the lateral part. Both the Q3Ds group and the L-group led to a similar VMC reorganization (Q3Ds vs. L-group, P > 0.05). In the Q2Ws group the VMC representation showed improvement in size (83.4% recovery compared with controls). The VMC showed recovery to normal output at 4 weeks after lesion (Control vs. Q4Ws group, P > 0.05). These results suggest that the VMC of the two hemispheres continuously interact through excitatory influences, preserving the normal output and inhibitory influences defining the border with the forelimb representation.
Asunto(s)
Mapeo Encefálico , Corteza Motora/fisiología , Red Nerviosa/fisiología , Plasticidad Neuronal/fisiología , Vibrisas/inervación , Anestésicos Locales/farmacología , Animales , Conducta Animal , Extremidades/inervación , Extremidades/fisiología , Lateralidad Funcional/efectos de los fármacos , Lateralidad Funcional/fisiología , Lidocaína/farmacología , Modelos Biológicos , Corteza Motora/efectos de los fármacos , Corteza Motora/lesiones , Red Nerviosa/efectos de los fármacos , Red Nerviosa/lesiones , Plasticidad Neuronal/efectos de los fármacos , Ácido Quinolínico/toxicidad , Ratas , Ratas Wistar , Factores de Tiempo , Vibrisas/fisiologíaRESUMEN
Clinical and laboratory studies have suggested that amphetamine treatment when paired with rehabilitation results in improved recovery of function after stroke or traumatic brain injury. In the present study, we investigated whether new anatomical pathways developed in association with improved motor function after brain damage and amphetamine treatment linked with rehabilitation. Following a unilateral sensorimotor cortex lesion in the adult rat, amphetamine (2 mg/kg) was administered in conjunction with physiotherapy sessions on postoperative days two and five. Physiotherapy was continued twice daily for the first 3 weeks after injury, and then once daily until week six. Performance on skilled forelimb reaching and ladder rung walking was used to assess motor improvement. Our results show that animals with sensorimotor cortical lesions receiving amphetamine treatment linked with rehabilitation had significant improvement in both tasks. Neuroanatomical tracing of efferent pathways from the opposite, non-damaged cortex resulted in the novel finding that amphetamine treatment linked with rehabilitation, significantly increased axonal growth in the deafferented basilar pontine nuclei. These results support the notion that pharmacological interventions paired with rehabilitation can enhance neuronal plasticity and thereby improve functional recovery after CNS injury.
Asunto(s)
Anfetamina/farmacología , Lesiones Encefálicas/tratamiento farmacológico , Lesiones Encefálicas/rehabilitación , Corteza Motora/efectos de los fármacos , Plasticidad Neuronal/efectos de los fármacos , Recuperación de la Función/efectos de los fármacos , Anfetamina/uso terapéutico , Animales , Axones/efectos de los fármacos , Axones/fisiología , Axones/ultraestructura , Biotina/análogos & derivados , Lesiones Encefálicas/fisiopatología , Estimulantes del Sistema Nervioso Central/farmacología , Estimulantes del Sistema Nervioso Central/uso terapéutico , Dextranos , Modelos Animales de Enfermedad , Vías Eferentes/efectos de los fármacos , Vías Eferentes/fisiología , Conos de Crecimiento/efectos de los fármacos , Conos de Crecimiento/fisiología , Conos de Crecimiento/ultraestructura , Masculino , Corteza Motora/lesiones , Corteza Motora/fisiopatología , Regeneración Nerviosa/efectos de los fármacos , Regeneración Nerviosa/fisiología , Plasticidad Neuronal/fisiología , Paresia/tratamiento farmacológico , Paresia/fisiopatología , Paresia/rehabilitación , Modalidades de Fisioterapia , Tractos Piramidales/efectos de los fármacos , Tractos Piramidales/fisiología , Ratas , Ratas Long-Evans , Recuperación de la Función/fisiología , Resultado del TratamientoRESUMEN
Chronically implanted microelectrodes have been an important tool used by neuroscientists for many years and are critical for the development of neural prostheses designed to restore function after traumatic central nervous system (CNS) injury. It is well established that a variety of mammals, including non-human primates (NHP), tolerate noble metal electrodes in the cortex for extended periods of time, but little is known about the long-term effects of electrode implantation at the cellular level. While data from rodents have clearly shown gliosis around such implants, there have been difficulties in demonstrating these reactions in NHP. Glial reactions are to be expected in NHP, since any trauma to the mammalian CNS is believed to result in the formation of a glial scar consisting of reactive astrocytes and microglia around the injury site. Because a glial scar can potentially affect the quality of recordings or stimulations from implanted electrodes, it is important to determine the extent of gliosis around implants in NHP. We studied the response of cortical glial cells to chronic electrode implantation in the motor cortices of Rhesus macaques (Macaca mulatta) after 3 months and 3 years duration. Antibodies specific for astrocytes and microglia were used to detect the presence of glial reactions around electrode implant sites. Reactive glia were found within the cortical neuropil surrounding the chronically implanted noble metal electrodes. Reactive gliosis persisted over the time periods studied and demonstrates the importance of developing strategies to minimize this event, even around noble metal implants.
Asunto(s)
Terapia por Estimulación Eléctrica/efectos adversos , Gliosis/etiología , Macaca mulatta/cirugía , Corteza Motora/lesiones , Corteza Motora/patología , Técnicas Estereotáxicas/efectos adversos , Animales , Astrocitos/citología , Astrocitos/fisiología , Biomarcadores/metabolismo , Cicatriz/etiología , Cicatriz/fisiopatología , Terapia por Estimulación Eléctrica/instrumentación , Electrodos Implantados/efectos adversos , Gliosis/fisiopatología , Metales/toxicidad , Microglía/citología , Microglía/fisiología , Corteza Motora/cirugía , Degeneración Nerviosa/etiología , Degeneración Nerviosa/fisiopatología , Proteínas del Tejido Nervioso/metabolismo , Neuronas/patología , Prótesis e Implantes/efectos adversos , Prótesis e Implantes/normas , Técnicas Estereotáxicas/instrumentación , Tiempo , Factores de TiempoRESUMEN
Dehydroepiandrosterone and its sulfate (DHEAS) are sex hormone precursors that exert marked neurotrophic and/or neuroprotective activity in the central nervous system. The present study evaluated the effects of DHEAS and 17beta-estradiol (E2) in a focal cortical cold lesion model, in which DHEAS (50 mg/kg, sc) and E2 (35 mg/kg, sc) were administered either as pretreatment (two subsequent injections 1 d and 1 h before lesion induction) or posttreatment (immediately after lesion induction). The focal cortical cold lesion was induced in the primary motor cortex by means of a cooled copper cylinder placed directly onto the cortical surface. One hour later, the animals were killed, the brains cut into 0.4-mm-thick slices, and the sections stained with 1% triphenyltetrazolium chloride. The volume of the hemispheric lesion was calculated for each animal. The results demonstrated that the lesion area was significantly attenuated in both the DHEAS- and E2- pre- and posttreated groups and that in the presence of letrozole, a nonsteroidal aromatase inhibitor, no neuroprotection was observed, suggesting that the beneficial effect of DHEAS on the cold injury might depend on the conversion of DHEAS to E2 within the brain. It is concluded that even a single posttraumatic administration of DHEAS may be of substantial therapeutic benefit in the treatment of focal brain injury with vasogenic edema.
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Lesiones Encefálicas/prevención & control , Frío , Deshidroepiandrosterona/administración & dosificación , Estradiol/fisiología , Corteza Motora/lesiones , Fármacos Neuroprotectores/administración & dosificación , Animales , Lesiones Encefálicas/etiología , Deshidroepiandrosterona/metabolismo , Esquema de Medicación , Estradiol/administración & dosificación , Masculino , Modelos Animales , Corteza Motora/efectos de los fármacos , Corteza Motora/metabolismo , Fármacos Neuroprotectores/metabolismo , Ratas , Ratas WistarRESUMEN
The learning and recalling of a lever-press task (LPT) after brief unilateral extradural compression (EC) of the right sensorimotor cortex was studied in Wistar rats. All rats, regardless of the time-point for EC, were trained to lever press for food from D(day)1 to D6. On D8, the position of the active lever was changed to the right side of the operant box and performance was tested until D14. Total and active lever presses, as well as % errors were used to analyse the performance. Rats submitted to EC 24 h before initiating the LPT schedule (naïve-compressed group) showed delayed task acquisition and impaired performance until D10. No significant impairments were detected by D3 on a beam-walking test, excluding paresis as the cause to the delay. Rats submitted to EC after they learned the LPT (trained-compressed group) showed only mildly impaired post-compression performance with no effects on the recalling of the task. Using a progressive ratio LPT, the maximum number of presses to obtain a food-pellet (breaking point) was significantly reduced 24h after EC suggesting reduced motivation for the task early after brain injury. The delayed acquisition of the LPT in naïve-compressed rats was accompanied by consistent cortical, striatal and thalamic degeneration detected by Fluoro-Jade and anti-glial fibrillary acidic protein (GFAP) staining, whereas the improvement in the performance of this group was accompanied by a reduction of the cortical damage on D10. Recall of the LPT in trained-compressed rats was not altered by EC, suggesting the contribution of compensatory mechanisms.
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Lesiones Encefálicas/fisiopatología , Condicionamiento Operante/fisiología , Recuerdo Mental/fisiología , Corteza Motora/fisiología , Desempeño Psicomotor/fisiología , Análisis de Varianza , Animales , Lesiones Encefálicas/patología , Duramadre , Lateralidad Funcional , Hipocampo/patología , Aprendizaje/fisiología , Masculino , Corteza Motora/lesiones , Corteza Motora/patología , Corteza Motora/fisiopatología , Neostriado/patología , Neuronas/patología , Ratas , Ratas Wistar , Estadísticas no Paramétricas , Tálamo/patologíaRESUMEN
We have treated undifferentiated mouse embryonic stem (ES) cells with all-trans retinoic acid (RA) to induce differentiation in vitro into neuron-like cells with good cell viability for use as a graft. Furthermore, we asked whether the RA-induced neuron-like cells restored neurological dysfunction. To this end, the cells were transplanted into right hemiplegia model of mice, developed by a cryogenic injury of motor cortex. Motor function of the recipients was gradually improved, whereas little improvement was observed in control mice. The lesion showed clustering of mature and almost mature neuron-like cells in mice transplanted with the RA-treated cells. The grafted cells had synaptic vesicles. This finding may suggest their maturation and synaptic connection in the recipient brain. Even though further study is necessary to elucidate molecular and cellular mechanisms responsible for the functional recovery, we consider that the ES cells may have advantage for use as a donor source in various neurological disorders including motor dysfunction.
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Lesiones Encefálicas/terapia , Trasplante de Tejido Encefálico , Neuronas/trasplante , Trasplante de Células Madre , Animales , Antineoplásicos/farmacología , Lesiones Encefálicas/patología , Diferenciación Celular/efectos de los fármacos , Endotelio Vascular/citología , Hemiplejía/patología , Hemiplejía/terapia , Ratones , Ratones Endogámicos C57BL , Corteza Motora/lesiones , Corteza Motora/patología , Movimiento , Neuronas/citología , Recuperación de la Función , Células Madre/citología , Células Madre/efectos de los fármacos , Tretinoina/farmacologíaRESUMEN
Loss of huntingtin-mediated brain-derived neurotrophic factor (BDNF) gene transcription has been described in Huntington's disease (HD) [Zuccato et al. (2001) Science, 293, 493-498]. It has been shown that BDNF is synthesized in the pyramidal layer of cerebral cortex and released in the striatum [Altar et al. (1997) Nature, 389, 856-860; Conner et al. (1997) J. Neurosci., 17, 2295-2313]. Here we show the cellular localization of BDNF in huntingtin-containing neurons in normal rat brain; our double-label immunofluorescence study shows that huntingtin and BDNF are co-contained in approximately 99% of pyramidal neurons of motor cortex. In the striatum, huntingtin is expressed in 75% of neurons containing BDNF. In normal striatum we also show that BDNF is contained in cholinergic and in NOS-containing interneurons, which are relatively resistant to HD degeneration. Furthermore, we show a reduction in huntingtin and in BDNF immunoreactivity in cortical neurons after striatal excitotoxic lesion. Our data are confirmed by an ELISA study of BDNF and by a Western blot analysis of huntingtin in cortex of quinolic acid (QUIN)-lesioned hemispheres. In the lesioned striatum we describe that the striatal subpopulation of cholinergic neurons, surviving degeneration, contain BDNF. The finding that BDNF is contained in nearly all neurons that contain huntingtin in the normal cortex, along with the reduced expression of BDNF after QUIN injection of the striatum, shows that huntingtin may be required for BDNF production in cortex.
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Lesiones Encefálicas/metabolismo , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Neuronas/metabolismo , Proteínas Nucleares/metabolismo , Ácido Quinolínico , Animales , Western Blotting , Lesiones Encefálicas/inducido químicamente , Lesiones Encefálicas/patología , Proteínas de Unión al Calcio/metabolismo , Recuento de Células , Colina O-Acetiltransferasa/metabolismo , Cuerpo Estriado/citología , Cuerpo Estriado/lesiones , Cuerpo Estriado/metabolismo , Modelos Animales de Enfermedad , Fosfoproteína 32 Regulada por Dopamina y AMPc , Ensayo de Inmunoadsorción Enzimática , Lateralidad Funcional , Humanos , Proteína Huntingtina , Enfermedad de Huntington/metabolismo , Enfermedad de Huntington/patología , Inmunohistoquímica , Masculino , Microscopía Confocal/instrumentación , Microscopía Confocal/métodos , Corteza Motora/citología , Corteza Motora/lesiones , Corteza Motora/metabolismo , Óxido Nítrico Sintasa/metabolismo , Fosfoproteínas/metabolismo , Ratas , Ratas WistarRESUMEN
Changes in somatosensory input can remodel human cortical motor organization, yet the input characteristics that promote reorganization and their functional significance have not been explored. Here we show with transcranial magnetic stimulation that sensory-driven reorganization of human motor cortex is highly dependent upon the frequency, intensity, and duration of stimulus applied. Those patterns of input associated with enhanced excitability (5 Hz, 75% maximal tolerated intensity for 10 min) induce stronger cortical activation to fMRI. When applied to acutely dysphagic stroke patients, swallowing corticobulbar excitability is increased mainly in the undamaged hemisphere, being strongly correlated with an improvement in swallowing function. Thus, input to the human adult brain can be programmed to promote beneficial changes in neuroplasticity and function after cerebral injury.
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Lesiones Encefálicas/rehabilitación , Terapia por Estimulación Eléctrica/métodos , Magnetismo/uso terapéutico , Corteza Motora/lesiones , Trastornos del Movimiento/rehabilitación , Plasticidad Neuronal/fisiología , Recuperación de la Función/fisiología , Adulto , Vías Aferentes/fisiología , Anciano , Anciano de 80 o más Años , Lesiones Encefálicas/patología , Lesiones Encefálicas/fisiopatología , Mapeo Encefálico , Tronco Encefálico/fisiología , Deglución/fisiología , Trastornos de Deglución/etiología , Trastornos de Deglución/fisiopatología , Trastornos de Deglución/rehabilitación , Femenino , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Corteza Motora/patología , Corteza Motora/fisiopatología , Trastornos del Movimiento/etiología , Trastornos del Movimiento/fisiopatología , Faringe/inervación , Faringe/fisiopatología , Estimulación Física , Tractos Piramidales/fisiología , Accidente Cerebrovascular/patología , Accidente Cerebrovascular/fisiopatología , Rehabilitación de Accidente CerebrovascularRESUMEN
Previous studies have utilized a lesion model of cortical injury that produces transient behavioral impairments to investigate the recovery of function process. To better understand the recovery process, it would be beneficial to use a lesion model that produces more severe, enduring, behavioral impairments. The purpose of experiment 1 was to validate whether large lesions of the sensorimotor cortex (SMC), which included the rostral forelimb and caudal forelimb regions, produced enduring behavioral deficits. Rats were given large unilateral electrolytic lesions of the SMC, administered either the N-methyl-D-aspartate (NMDA) antagonist, MK-801 or saline 16 h after injury, and tested on a battery of behavioral tests. Enduring behavioral deficits were observed, for at least 6 months, on two tests of forelimb placing while transient deficits were observed on the foot-fault and somatosensory neutralization tests. Administration of MK-801 facilitated recovery on the somatosensory neutralization test; however, it did not induce recovery on either forelimb placing test. A second experiment was performed to determine if earlier administration of MK-801, the NMDA antagonist magnesium chloride (MgCl(2)), or the anti-oxidant N-tert-butyl-alpha-phenylnitrone (PBN) could induce behavioral recovery in this chronic model. Treatment with these drugs induced behavioral recovery on the forelimb placing tests, whereas, the saline-treated rats did not show any signs of behavioral recovery for at least 3 months. Anatomical analysis of the striatum showed that MK-801 and MgCl(2) but not PBN reduced the extent of lesion-induced striatal atrophy. These results suggest that administration of MK-801, MgCl(2), or PBN shortly after cortical injury can induce recovery of function when recovery is otherwise not expected in un-treated rats.