RESUMEN
The increasing consumption of cleaning products deteriorates water resources due to harmful components such as phosphorus (P) and nitrogen (N) compounds, oils, bleach, and acids, typical compounds in traditional detergents. The use of biodegradable detergents as an environmentally friendly alternative has been proposed in different regions. In Colombia, resolution 1770/2018 sets a minimum biodegradability rate of 60% for the surfactants present in liquid detergents, which would reduce to a similar extent the impacts on water after their use. However, the environmental impacts of the supply chain of these detergents and their raw materials have not been evaluated so far. This study presents an environmental life cycle assessment of petroleum-based liquid detergents and a comparison to traditional solid detergents, based on the ISO 14040 standard and the ReCiPe-2016 impacts assessment method. A novel bio-detergent containing anionic plant-based surfactants was proposed in this analysis. The impacts of packaging and the distribution of the product to consumers were also considered. Raw materials contributed to 91% of the total of 314 g of CO2 eq generated per liter of liquid detergent, where the production of fatty alcohol sulfate and PET packaging shared 78.8% and 12.2% of the total impact, respectively. It was also determined that 5.4 L of water are consumed and 0.09 g of P eq and 0.1 g of N eq are emitted per liter of detergent. This liquid detergent presented better environmental performance than traditional detergents in all the impact categories, except for the fossil resource scarcity category. The evaluated detergent would significantly mitigate the generation of negative effects on ecosystems. Moreover, the substitution of PET for HDPE packaging could reduce the impacts on freshwater eutrophication by 10%, although the carbon footprint can slightly increase, which could be compensated due to its higher recyclability rate. In contrast, the proposed bio-detergent would not have significant benefits and would negatively affect water consumption and land use in its supply chain.
Asunto(s)
Detergentes , Ambiente , Tensoactivos/química , Detergentes/química , Colombia , Plantas/química , Petróleo , Factores de Tiempo , Embalaje de ProductosRESUMEN
Over the past 10 years we have been developing a multi-attribute analytical platform that allows for the preparation of milligram amounts of functional, high-pure, and stable Torpedo (muscle-type) nAChR detergent complexes for crystallization purpose. In the present work, we have been able to significantly improve and optimize the purity and yield of nicotinic acetylcholine receptors in detergent complexes (nAChR-DC) without compromising stability and functionality. We implemented new methods in the process, such as analysis and rapid production of samples for future crystallization preparations. Native nAChR was extracted from the electric organ of Torpedo californica using the lipid-like detergent LysoFos Choline 16 (LFC-16), followed by three consecutive steps of chromatography purification. We evaluated the effect of cholesteryl hemisuccinate (CHS) supplementation during the affinity purification steps of nAChR-LFC-16 in terms of receptor secondary structure, stability and functionality. CHS produced significant changes in the degree of ß-secondary structure, these changes compromise the diffusion of the nAChR-LFC-16 in lipid cubic phase. The behavior was reversed by Methyl-ß-Cyclodextrin treatment. Also, CHS decreased acetylcholine evoked currents of Xenopus leavis oocyte injected with nAChR-LFC-16 in a concentration-dependent manner. Methyl-ß-Cyclodextrin treatment do not reverse functionality, however column delipidation produced a functional protein similar to nAChR-LFC-16 without CHS treatment.
Asunto(s)
Ésteres del Colesterol/química , Proteínas de Peces/química , Receptores Nicotínicos/química , Acetilcolina/farmacología , Animales , Detergentes/química , Potenciales Evocados/efectos de los fármacos , Proteínas de Peces/aislamiento & purificación , Proteínas de Peces/metabolismo , Oocitos/fisiología , Conformación Proteica en Lámina beta , Receptores Nicotínicos/aislamiento & purificación , Receptores Nicotínicos/metabolismo , Torpedo/metabolismo , Xenopus laevis/crecimiento & desarrollo , Xenopus laevis/metabolismo , beta-Ciclodextrinas/químicaRESUMEN
Lipase based formulations has been a rising interest to laundry detergent industry for their eco-friendly property over phosphate-based counterparts and compatibility with chemical detergents ingredients. A thermo-stable Anoxybacillus sp. ARS-1 isolated from Taptapani Hotspring, India was characterized for optimum lipase production employing statistical model central composite design (CCD) under four independent variables (temperature, pH, % moisture and bio-surfactant) by solid substrate fermentation (SSF) using mustard cake. The output was utilized to find the effect of parameters and their interaction employing response surface methodology (RSM). A quadratic regression with R2 = 0.955 established the model to be statically best fitting and a predicted highest lipase production of 29.4 IU/g at an optimum temperature of 57.5 °C, pH 8.31, moisture 50% and 1.2 mg of bio-surfactant. Experimental production of 30.3 IU/g lipase at above conditions validated the fitness of model. Anoxybacillus sp. ARS-1 produced lipase was found to resist almost all chemical detergents as well as common laundry detergent, proving it to be a prospective additive for incorporation.
Asunto(s)
Anoxybacillus/enzimología , Proteínas Bacterianas/biosíntesis , Detergentes/química , Lipasa/biosíntesis , Modelos Estadísticos , Anoxybacillus/genética , ADN Bacteriano/genética , Detergentes/farmacología , Estabilidad de Enzimas/efectos de los fármacos , Fermentación , Concentración de Iones de Hidrógeno , India , Planta de la Mostaza/química , Filogenia , Aceites de Plantas/química , ARN Ribosómico 16S/genética , TemperaturaRESUMEN
A newly isolated culture, Pseudomonas guariconesis, is reported for the first time for lipase production. Various process parameters affecting enzyme production were optimized through statistical design experiments. The Plackett-Burman experimental design was used for screening 10 parameters for lipase production, which was further optimized using the central composite design of response surface methodology. Maximum lipase activity of 220 U/ml was obtained after 24 h of incubation in shake-flask cultures with an inoculum concentration of 0.6% v/v, incubation temperature of 30°C, and medium pH 9.0. Castor oil (0.5% v/v) was used as the inducer for lipase production. The enzyme was found to be compatible with five different commercial detergents, indicating its potential to be used in detergent formulations. It also acted as a biocatalyst in a transesterification process. The alkaline enzyme was found to be stable in the presence of bleaching agents, metal ions, and organic solvents as well.
Asunto(s)
Detergentes/química , Lipasa/metabolismo , Pseudomonas/enzimología , Biocatálisis , Aceite de Ricino/metabolismo , Estabilidad de Enzimas , Esterificación , Fermentación , Concentración de Iones de Hidrógeno , Lipasa/biosíntesis , Solventes , TemperaturaRESUMEN
Protein solubility is a critical prerequisite to any proteomics analysis. Combination of urea/thiourea and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) have been routinely used to enhance protein solubilization for oil palm proteomics studies in recent years. The goals of these proteomics analysis are essentially to complement the knowledge regarding the regulation networks and mechanisms of the oil palm fatty acid biosynthesis. Through omics integration, the information is able to build a regulatory model to support efforts in improving the economic value and sustainability of palm oil in the global oil and vegetable market. Our study evaluated the utilization of sodium deoxycholate as an alternative solubilization buffer/additive to urea/thiourea and CHAPS. Efficiency of urea/thiourea/CHAPS, urea/CHAPS, urea/sodium deoxycholate and sodium deoxycholate buffers in solubilizing the oil palm (Elaeis guineensis var. Tenera) mesocarp proteins were compared. Based on the protein yields and electrophoretic profile, combination of urea/thiourea/CHAPS were shown to remain a better solubilization buffer and additive, but the differences with sodium deoxycholate buffer was insignificant. A deeper mass spectrometric and statistical analyses on the identified proteins and peptides from all the evaluated solubilization buffers revealed that sodium deoxycholate had increased the number of identified proteins from oil palm mesocarps, enriched their gene ontologies and reduced the number of carbamylated lysine residues by more than 67.0%, compared to urea/thiourea/CHAPS buffer. Although only 62.0% of the total identified proteins were shared between the urea/thiourea/CHAPS and sodium deoxycholate buffers, the importance of the remaining 38.0% proteins depends on the applications. The only observed limitations to the application of sodium deoxycholate in protein solubilization were the interference with protein quantitation and but it could be easily rectified through a 4-fold dilution. All the proteomics data are available via ProteomeXchange with identifier PXD013255. In conclusion, sodium deoxycholate is applicable in the solubilization of proteins extracted from oil palm mesocarps with higher efficiency compared to urea/thiourea/CHAPS buffer. The sodium deoxycholate buffer is more favorable for proteomics analysis due to its proven advantages over urea/thiourea/CHAPS buffer.
Asunto(s)
Arecaceae/química , Ácido Desoxicólico/química , Detergentes/química , Aceite de Palma/química , Proteínas de Plantas/análisis , Proteómica , Tampones (Química) , Ácidos Cólicos/química , Proteínas de Plantas/químicaRESUMEN
Shampoo is a hair care product designed to clean the skin and hair of the scalp. Among the ingredients that go into the making of a shampoo are detergents, conditioners, thickeners, sequestering agents, pH adjusters, preservatives, and active ingredients such as anti-dandruff agents. The purpose of this study was to identify the composition of 140 shampoos available in pharmacies, in stores of a mass-market chain, or from mail-order retailers. Forty-one shampoos were advertised as "gentle", 12 as specially formulated for infants, 35 as anti-dandruff, and 52 without any particular claim. We analysed the cleansing base, preservatives, and anti-dandruff agents when relevant and identified the allergens regardless of whether or not they are listed under Regulation (EC) No. 1223/2009 as one of the 26 regulated substances. We discovered that unlike shampoos sold in stores of a mass-market chain and those available from mail-order retailers, those sold in pharmacies expose users to some of the 26 substances listed under Regulation (EC) No. 1223/2009. We also determined that baby shampoos sold in pharmacies are allergen-free. Regarding anti-dandruff formulations, the largest variety of active ingredients was found in shampoos sold in pharmacies. Overall, the most common active ingredients were olamines, zinc pyrithione, azoles, selenium disulphide, and plant extracts. Shampoos sold in pharmacies appear to contain fewer allergens listed under Regulation (EC) No. 1223/2009 compared to those sold elsewhere.
Asunto(s)
Fármacos Dermatológicos/química , Detergentes/química , Preparaciones para el Cabello/química , Cabello/efectos de los fármacos , Humanos , Cuero Cabelludo/efectos de los fármacosRESUMEN
Introduction: Liquid laundry detergent capsules (also called single-use detergent sacs; laundry pods; laundry packets) have become an increasingly popular household product worldwide. Objectives: To review the composition and mechanisms of toxicity of liquid laundry detergent, capsules, and the circumstances, routes, clinical features (and impact of packaging changes) and management of exposure. Methods: The databases PubMed and EMBASE were searched using the terms: "detergent" and "capsule", "pod", "pac" or "sac" combined with "poison", "ingest", "expos" but not "animal" or "in vitro" or "bacteria". The searches yielded 289 articles, of which 186 were excluded: 38 duplicates, 133 not relevant, 10 abstracts which had been published as a paper and 5 non-English language articles. The bibliographies of relevant articles were hand-searched which yielded 14 additional citations. Searching of abstracts from scientific meetings produced five additional citations. A total of 122 publications were relevant to the objectives of the review. Capsules and composition: Capsules typically contain anionic surfactants (20-35%), non-ionic surfactants (10-20%), propylene glycol (8-20%) and ethanol (2-5%) within a water-soluble polyvinyl alcohol membrane. Mechanisms of toxicity: Non-ionic surfactants are the primary mechanism, though anionic surfactants, ethanol and propylene glycol may also contribute. Circumstances of exposure: The majority (60%) of children are exposed when the capsule is removed from its original container. Routes of exposure: Ingestion is the most common (>85%); ocular (<15%) and dermal (<8%) exposure account for the remainder. Features following ingestion: Features develop in around half of all exposures, though >90% are minor. In those with features, vomiting occurs in some 50%; coughing and drowsiness are reported in <5%. Respiratory depression (<0.5%), central nervous system depression (<0.1%) esophageal or gastric injury (<0.5%), metabolic acidosis and hyperlactatemia (<0.05%) have been reported rarely. Of 17 deaths reported, 13 were adults and nine were suffering from cognitive impairment. Features following ocular exposure: Conjunctivitis, eye irritation and/or eye pain are commonly experienced; corneal injury is less common but complete recovery typically occurs within one week. Features following dermal exposure: Clinically important dermal toxicity seldom occurs, though skin burns can develop in <5% of cases when skin contact is prolonged. Impact of packaging changes on features: The implementation of packaging changes resulted in a fall in the number of exposures and their severity in the United States and in the number in Italy. Management-ingestion: Gut decontamination is not recommended, though small amounts of fluid can be administered orally to rinse out the mouth. Symptomatic and supportive care should be offered to all patients that develop features of toxicity. Supplemental oxygen should be administered for hypoxemia, and bronchodilators for laryngospasm/bronchospasm. Intubation and assisted ventilation may be required if CNS and respiratory depression develop. A chest radiograph should be performed if respiratory features develop. In patients with swallowing difficulties, drooling or oropharyngeal burns, endoscopy should be performed; if substantial mucosal damage is present MRI should be considered. In addition, intravenous fluids will be required if prolonged vomiting or diarrhea occur and acid-base disturbances should be corrected. Management-eye exposure: Thorough irrigation of the eye with sodium chloride 0.9% is required. Instillation of a local anesthetic will reduce discomfort and help more thorough decontamination. Due to the potential for corneal injury, fluorescein should be instilled. If ocular injury is present, the patient should be referred to an ophthalmologist. Management-skin exposure: Skin should be irrigated thoroughly with soap and water, and burns should be treated as a thermal burn. Conclusions: Accidental ingestion usually produces no or only minor features. Very rarely respiratory depression, central nervous system depression, esophageal or gastric injury, hyperlactatemia and metabolic acidosis occur. Ocular exposure results in corneal injury infrequently and skin burns can develop uncommonly following prolonged dermal contact. Of 17 deaths reported, 13 were adults and nine were suffering from cognitive impairment.
Asunto(s)
Detergentes/química , Detergentes/toxicidad , Acidosis/inducido químicamente , Cápsulas/química , Preescolar , Detergentes/envenenamiento , Ingestión de Alimentos , Europa (Continente) , Humanos , Intubación , América del Norte , Intoxicación/mortalidad , Intoxicación/terapia , Convulsiones/inducido químicamente , Tensoactivos/química , Tensoactivos/toxicidadRESUMEN
An extracellular lipase from Aureobasidium pullulans was obtained and purified with a specific activity of 17.7 U/mg of protein using ultrafiltration and a DEAE-Sepharose Fast Flow column. Characterization of the lipase indicated that it is a novel finding from the species A. pullulans. The molecular weight of the lipase was 39.5 kDa, determined by sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme exhibited its optimum activity at 40 °C and pH of 7. It also showed a remarkable stability in some organic solutions (30%, v/v) including n-propanol, isopropanol, dimethyl sulfoxide (DMSO), and hexane. The catalytic activity of the lipase was enhanced by Ca2+ and was slightly inhibited by Mn2+ and Zn2+ at a concentration of 10 mmol/L. The lipase was activated by the anionic surfactant SDS and the non-ionic surfactants Tween 20, Tween 80, and Triton X-100, but it was drastically inhibited by the cationic surfactant cetyl trimethyl ammonium bromide (CTAB). Furthermore, the lipase was able to hydrolyze a wide variety of edible oils, such as peanut oil, corn oil, sunflower seed oil, sesame oil, and olive oil. Our study indicated that the lipase we obtained is a potential biocatalyst for industrial use.
Asunto(s)
Ascomicetos/enzimología , Proteínas Fúngicas/química , Glucanos/química , Lipasa/química , Calcio , Catálisis , Aceite de Maíz/metabolismo , Detergentes/química , Estabilidad de Enzimas , Hexanos/química , Concentración de Iones de Hidrógeno , Hidrólisis , Microbiología Industrial , Manganeso/química , Aceite de Oliva/metabolismo , Aceite de Cacahuete/metabolismo , Aceite de Sésamo/metabolismo , Especificidad por Sustrato , Aceite de Girasol/metabolismo , Tensoactivos , Temperatura , Zinc/químicaRESUMEN
BACKGROUND: Chrysin is a strong inhibitor of breast cancer resistance protein (BCRP) but it is practically insoluble in water. Effective solubilization of chrysin is critical for its pharmaceutical application as an absorption enhancer via inhibition of BCRP-mediated drug efflux. OBJECTIVE: This study aimed to develop an effective oral formulation of chrysin to improve its in vivo effect as an absorption enhancer. METHOD: Solid dispersions (SDs) of chrysin were prepared with hydrophilic carriers having surface acting properties and a pH modulator. In vitro and in vivo characterizations were performed to select the optimal SDs of chrysin. RESULTS: SDs with Brij®L4 and aminoclay was most effective in increasing the solubility of chrysin by 13-53 fold at varying drug-carrier ratios. Furthermore, SDs significantly improved the dissolution rate and extent of drug release. SDs (chrysin: Brij®L4: aminoclay=1:3:5) achieved approximately 60% and 83% drug release within 1 h and 8 h, respectively, in aqueous medium, while the dissolution of the untreated chrysin was less than 13%. XRD patterns indicated the amorphous state of chrysin in SDs. The SD formulation was effective in improving the bioavailability of topotecan, a BCRP substrate in rats. Following oral administration of topotecan with the SDs of chrysin, the Cmax and AUC of topotecan was enhanced by approximately 2.6- and 2-fold, respectively, while the untreated chrysin had no effect. CONCLUSION: The SD formulation of chrysin with Brij®L4 and aminoclay appeared to be promising in improving the dissolution of chrysin and enhancing its in vivo effect as an absorption enhancer.
Asunto(s)
Transportador de Casetes de Unión a ATP, Subfamilia G, Miembro 2/antagonistas & inhibidores , Protocolos de Quimioterapia Combinada Antineoplásica/farmacología , Flavonoides/farmacología , Absorción Intestinal/efectos de los fármacos , Neoplasias/tratamiento farmacológico , Transportador de Casetes de Unión a ATP, Subfamilia G, Miembro 2/metabolismo , Administración Oral , Animales , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Disponibilidad Biológica , Arcilla/química , Detergentes/química , Composición de Medicamentos/métodos , Evaluación Preclínica de Medicamentos , Flavonoides/química , Flavonoides/uso terapéutico , Células HT29 , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Masculino , Polidocanol/química , Ratas , Ratas Sprague-Dawley , Solubilidad , Topotecan/farmacología , Topotecan/uso terapéutico , Agua/químicaRESUMEN
This article addresses the social and environmental aspects related to the presence of phosphorus in surface water bodies, assessing the impacts caused by the utilization of powder detergents on the quality and availability of water resources, with particular respect to the occurrence of eutrophication. Based on data concerning the composition and consumption of the products currently sold in Brazil, the evolution of this phenomenon leading to the current situation is reported. Indications are that Brazilian detergents have a low phosphorus content, thus it is possible to infer that these products offer a small risk of impacting the trophic levels of surface waters. There is, however, a need to adjust the content of legislation to market practices, as well as improve public policies related to environmental education. By means of an intensive process of communication and education, awareness about the issue can be raised, keeping this major source of phosphorus in the environment under control and contributing to the increase in the availability of water, through the prevention of eutrophication processes.
Este artigo aborda a interface social e ambiental da presença de fósforo nas águas de abastecimento, dimensionando os impactos ocasionados pelo uso de detergentes fosfatados em pó sobre a qualidade e a disponibilidade dos recursos hídricos, no tocante à ocorrência da eutrofização. Com base em dados a respeito da formulação e consumo dos produtos comercializados no Brasil, relata a evolução do assunto e apresenta o retrato da situação atual. Indica que os detergentes brasileiros possuem, hoje, baixa concentração de fósforo, de forma que representam um reduzido risco de impacto sobre o nível trófico das águas. Pontua, entretanto, a necessidade de ajustar o conteúdo da legislação à prática do mercado e de aprimorar as políticas públicas relacionadas com a educação ambiental. Mediante um processo efetivo de comunicação e educação, será possível conscientizar a população acerca do assunto, contribuindo para a manutenção do controle dessa importante fonte de fósforo no meio ambiente e para o aumento da disponibilidade hídrica, por meio da prevenção dos processos de eutrofização.
Asunto(s)
Detergentes/química , Monitoreo del Ambiente/métodos , Fósforo/análisis , Salud Pública , Brasil , Eutrofización , Humanos , Contaminantes Químicos del Agua/análisisRESUMEN
Antimalarial drug discovery expands on targeted and phenotype-based screening of potential inhibitory molecules to ascertain overall efficacy, phenotypic characteristics and toxicity, prior to exploring pharmacological optimizations. Candidate inhibitors may have varying chemical properties, thereby requiring specific reconstitution conditions to ensure solubility, stability or bioavailability. Hence, a variety of solvents, buffers, detergents and stabilizers become part of antimalarial efficacy assays, all of which, above certain threshold could interfere with parasite viability, invasion or red blood cell properties leading to misinterpretation of the results. Despite their routine use across malaria research laboratories, there is no documentation on non-toxic range for common constituents including DMSO, glycerol, ethanol and methanol. We herein constructed a compatibility reference guide for 14 such chemicals and estimated their Permissible Limit against P. falciparum asexual stages at which viability and replication of parasites are not compromised. We also demonstrate that at the estimated Permissible Limit, red blood cells remain healthy and viable for infection by merozoites. Taken together, this dataset provides a valuable reference tool for the acceptable concentration range for common chemicals during in vitro antimalarial tests.
Asunto(s)
Antimaláricos/toxicidad , Detergentes/toxicidad , Descubrimiento de Drogas/métodos , Eritrocitos/efectos de los fármacos , Excipientes/toxicidad , Plasmodium falciparum/efectos de los fármacos , Solventes/toxicidad , Antimaláricos/química , Antimaláricos/farmacología , Tampones (Química) , Detergentes/química , Evaluación Preclínica de Medicamentos , Eritrocitos/parasitología , Excipientes/química , Humanos , Malaria/tratamiento farmacológico , Solventes/químicaRESUMEN
Curcumin, the principal curcuminoid in the food spice turmeric, is insoluble in water at room temperature. We have previously solubilized curcumin in water with the application of heat (100 °C) and found that this solubilized curcumin could be used as a gel protein stain. However, heat solubilization in water solubilized only a small fraction of the curcuminoid (1.5%), making it relatively expensive to stain one gel ($1.5-2 per gel). Heat/pressure solubilization (121 °C/15 psi) helps increase the solubility of curcumin in water to only 5%. Therefore, we ventured into increasing curcumin solubility further by using detergents (Tween 20, Tween 80, or Triton X-100) combined with heat/pressure treatment. Curcumin solubilized with Tween 20, Tween 80 or Triton X-100 stained proteins efficiently as before. We could dramatically increase the solubility of curcumin by solubilizing in detergents such that we had to dilute the solubilized curcumin 1:100 with water to stain proteins. However, we had to use the soluble curcumin (extracted with heat/pressure alone) straight without dilution. Thus, the amount of curcumin solubilized with detergents was at least 100-fold, with 0.5% Tween 80 being the most efficient. Staining can be carried out in 30 min, and sensitivity of staining is similar or better than that obtained with Coomassie Brilliant Blue (CBB). It is unnecessary to destain the gel, and excess curcumin can be discarded into the sink, unlike CBB. Curcumin solubilized in Tween 20 has been found to be stable for a period of over 4 years. Therefore, curcumin solubilized in Tween 80 or Triton X-100 can serve as an efficient nontoxic and environment-friendly protein stain. Tween 80 has been used in cough syrups to make a colloidal suspension, and also in foods. Solubilizing curcumin with Tween 80 would be a useful alternative to increase curcumin bioavailability in clinical studies.
Asunto(s)
Curcumina , Detergentes , Calor , Presión , Proteínas , Animales , Curcumina/química , Detergentes/química , Electroforesis en Gel de Poliacrilamida , Octoxinol , Polisorbatos , Estabilidad Proteica , Proteínas/análisis , Proteínas/química , Colorantes de Rosanilina , Solubilidad , Coloración y Etiquetado/métodosRESUMEN
Membrane proteins (MP) are stable in their native lipid environment. To enable structural and functional investigations, MP need to be extracted from the membrane. This is a critical step that represents the main obstacle for MP biochemistry and structural biology. General guidelines and rules for membrane protein solubilization remain difficult to establish. This review aims to provide the reader with a comprehensive overview of the general concepts of MP solubilization and stabilization as well as recent advances in detergents innovation. Understanding how solubilization and stabilization are intimately linked is key to facilitate MP isolation toward fundamental structural and functional research as well as drug discovery applications. How to manage the tour de force of destabilizing the lipid bilayer and stabilizing MP at the same time is the holy grail of successful isolation and investigation of such a delicate and fascinating class of proteins.
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Proteínas de la Membrana/química , Yin-Yang , Detergentes/química , Proteínas de la Membrana/aislamiento & purificación , Estabilidad Proteica , Proteínas Recombinantes/química , SolubilidadRESUMEN
Psychrotolerant yeast Rhodotorula sp. Y-23 was isolated from the sediment core sub-samples of Nella Lake, East Antarctica. Isolate was screened for lipase production using plate assay method followed by submerged fermentation. Production optimization revealed the maximum lipase production by using palmolein oil (5% v/v), pH 8.0 and inoculum size of 2.5% v/v at 15 °C. The potential inducers for lipase were 1% w/v of galactose and KNO3 , and MnCl2 (0.1% w/v). Final productions with optimized conditions gave 5.47-fold increase in lipase production. Dialyzed product gave a purification fold of 5.63 with specific activity of 26.83 U mg-1 and 15.67% yields. This lipase was more stable at pH 5.0 and -20 °C whereas more activity was found at pH 8.0 and 35 °C. Stability was more in 50 mM Fe3+ , EDTA-Na (20 mM), sodium deoxycholate (20 mM), H2 O2 (1% v/v), and almost all organic solvents (50% v/v). Tolerance capacity at wider range of pH and temperature with having lower Km value i.e., 0.08 mg ml-1 and higher Vmax 385.68 U mg-1 at 15 °C make the studied lipase useful for industrial applications. Besides this, the lipase was compatible with commercially available detergents, and its addition to them increases lipid degradation performances making it a potential candidate in detergent formulation.
Asunto(s)
Frío , Proteínas Fúngicas/metabolismo , Sedimentos Geológicos/microbiología , Lipasa/metabolismo , Rhodotorula/enzimología , Solventes/farmacología , Regiones Antárticas , Detergentes/química , Estabilidad de Enzimas/efectos de los fármacos , Proteínas Fúngicas/biosíntesis , Proteínas Fúngicas/aislamiento & purificación , Proteínas Fúngicas/fisiología , Concentración de Iones de Hidrógeno , Cinética , Lipasa/biosíntesis , Lipasa/aislamiento & purificación , Lipasa/fisiología , Aceites de Plantas/metabolismo , Rhodotorula/clasificación , Rhodotorula/genética , Rhodotorula/aislamiento & purificación , Especificidad por Sustrato , TemperaturaRESUMEN
This work seeks to discuss the presence of phosphorus in raw sewage considering the formulation of the powder detergent for cleaning fabrics currently sold in Brazil. Based on the results of laboratory analyses performed in the sewage inflows to three wastewater treatment plants (WWTPs) located in São Paulo state, it evaluates the different fractions of this element and sizes the impact caused by the product. The average concentration of total phosphorus (total-P) in sewage inflows has shown a reduction trend over the years, and it is currently between 5.3 and 7.6â mg/L. The participation of organic phosphorus (org-P) tends to be higher than that of the inorganic phosphorus (inorg-P) with average around 64% of total-P. This situation indicates a change from the default and it may be influenced by the reduced contribution of phosphorus in the powder detergents. It was concluded that the formulation of the Brazilian products, which have a very low phosphate content, less than 0.01% by weight, may have altered the phosphorus dynamics in sewage. In other respects, results have shown the need to enhance actions aimed at controlling the sources of phosphorus in sewage, with a view to assure preventive measures to water pollution processes.
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Detergentes/química , Fósforo/química , Aguas Residuales , Brasil , Aguas del Alcantarillado , Purificación del AguaRESUMEN
A simple nonionic detergent - based vortex- synchronized matrix solid-phase dispersion (ND-VSMSPD) method was developed to extract bioactive compounds in Forsythiae Fructus coupled with ultra high-performance liquid chromatography (UHPLC). Nonionic detergent Triton 114 was firstly used as a green elution reagent in vortex- synchronized MSPD procedure. The optimum parameters were investigated to attain the best results, including Florisil as sorbent, 2mL 10% (v/v) nonionic detergent Triton X-114 as the elution reagent, 1:1 of sample/sorbent ratio, grinding for 3min, and whirling for 2min. The recoveries of the six compounds in Forsythiae Fructus were in the range of 95-104% (RSD <4.6%) and the method displayed a good linearity within the range of 0.08-20µgmL-1 for caffeic acid, 0.6-150µgmL-1 for forsythoside A, 0.4-100µgmL-1 phillyrin, 0.2-50µgmL-1 for quercetin, isorhamnetin and arctigenin (r≥0.999). It was proved that the extraction yields of almost all compounds attained by the established vortex- synchronized MSPD, which required lower sample, reagent and time, were higher than the normal MSPD and the traditional ultrasonic-assisted extraction. Consequently, this developed vortex- synchronized MSPD coupled with simple UHPLC method could be efficiently applies to extract and analyze the target compounds in real Forsythiae Fructus samples.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Forsythia/química , Extractos Vegetales/química , Polietilenglicoles/química , Detergentes/química , Frutas , Octoxinol , Extractos Vegetales/análisis , Extracción en Fase Sólida/métodos , Factores de TiempoRESUMEN
BACKGROUND: Camellia sinensis var. assamica seed cake (a by-product of tea-seed oil) is an abundant resource with poor utilisation. C. sinensis var. assamica seed saponin (CSS) is one kind of non-ionic surfactant. In this study, the CSS extraction conditions were optimised by response surface methodology (RSM) and then the CSS detergent was developed. Additionally, the safety and decontamination ability of the developed detergent were evaluated. RESULTS: The optimised extraction conditions were including the extracting temperature of 40.04 °C, extraction time of 4.97 h, ethanol concentration of 64.11% and liquid-solid ratio of 14.57:1 mL g-1 . The formula of the CSS detergent was as follows: 20% crude CSS, 0.3% oxidised tea polyphenols (OTPs), 0.2% nisin, 0.3% sodium dehydroacetate, 0.7% sodium alginate and 0.5% sodium polyacrylate. The LD50 of the CSS detergent exceeds 14 g kg-1 in mice, indicating the detergent was non-toxic. Both of the emulsifying and the pesticide residues removal abilities of the CSS detergent were significantly stronger than the commercial detergent. CONCLUSION: A natural tea seed saponin detergent with good safety and decontamination ability was successfully developed. This can make better use of the tea seed cake, thereby creating added value in the tea seed oil industry. © 2017 Society of Chemical Industry.
Asunto(s)
Camellia sinensis/química , Detergentes/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación , Saponinas/aislamiento & purificación , Detergentes/química , Extractos Vegetales/química , Polifenoles/química , Saponinas/análisis , Saponinas/química , Semillas/química , Residuos/análisisRESUMEN
Resumo Este artigo aborda a interface social e ambiental da presença de fósforo nas águas de abastecimento, dimensionando os impactos ocasionados pelo uso de detergentes fosfatados em pó sobre a qualidade e a disponibilidade dos recursos hídricos, no tocante à ocorrência da eutrofização. Com base em dados a respeito da formulação e consumo dos produtos comercializados no Brasil, relata a evolução do assunto e apresenta o retrato da situação atual. Indica que os detergentes brasileiros possuem, hoje, baixa concentração de fósforo, de forma que representam um reduzido risco de impacto sobre o nível trófico das águas. Pontua, entretanto, a necessidade de ajustar o conteúdo da legislação à prática do mercado e de aprimorar as políticas públicas relacionadas com a educação ambiental. Mediante um processo efetivo de comunicação e educação, será possível conscientizar a população acerca do assunto, contribuindo para a manutenção do controle dessa importante fonte de fósforo no meio ambiente e para o aumento da disponibilidade hídrica, por meio da prevenção dos processos de eutrofização.
Abstract This article addresses the social and environmental aspects related to the presence of phosphorus in surface water bodies, assessing the impacts caused by the utilization of powder detergents on the quality and availability of water resources, with particular respect to the occurrence of eutrophication. Based on data concerning the composition and consumption of the products currently sold in Brazil, the evolution of this phenomenon leading to the current situation is reported. Indications are that Brazilian detergents have a low phosphorus content, thus it is possible to infer that these products offer a small risk of impacting the trophic levels of surface waters. There is, however, a need to adjust the content of legislation to market practices, as well as improve public policies related to environmental education. By means of an intensive process of communication and education, awareness about the issue can be raised, keeping this major source of phosphorus in the environment under control and contributing to the increase in the availability of water, through the prevention of eutrophication processes.
Asunto(s)
Humanos , Fósforo/análisis , Monitoreo del Ambiente/métodos , Salud Pública , Detergentes/química , Contaminantes Químicos del Agua/análisis , Brasil , EutrofizaciónRESUMEN
Liquid detergents are more convenient than powdered detergents as they dissolve readily in water, generate less dust and dosing is easy. However, the stability of liquid detergents is an issue of concern. Therefore, the objective of this research is to study the formulation requirement to produce heavy-duty liquid detergents based on palm-based methyl esters sulphonate (MES) with desirable properties and performance. MES is produced from renewable and sustainable feedstock suitable to replace the conventional fossil-based surfactant, linear alkyl benzene sulphonates (LAS). Five palm-based liquid detergents (PBLDs) were formulated using C16/18 MES as the primary surfactant. The physical properties, washing performance, stability and biodegradability of PBLDs were evaluated. Performance of the PBLDs was evaluated against two commercial liquid detergents which use LAS and alcohol glucoside as surfactant (benchmark product) and it was found that the PBLDs exhibited excellent performance. PBLDs can be formulated with or without phosphates and still demonstrate good detergency. The stability study of PBLDs indicated that no appreciable hydrolysis occurred. PBLDs exhibited better biodegradability profiles compared to commercial detergent containing LAS. PBLDs passed the 60% biodegradability level within 3 to 8 d, while commercial detergent took 24 d. It was shown that palm-based C16/18 MES could be potentially formulated into liquid detergents and gave better performance than LAS based liquid detergent. Attributes of C16/18 MES should not be overlooked, which include an abundant and naturally derived palm stearin as raw material and environmental safety profiles that are superior to most synthetic surfactants.
Asunto(s)
Alcanosulfonatos/química , Detergentes/química , Ésteres/química , Aceites de Plantas/química , Biodegradación Ambiental , Fenómenos Químicos , Química Farmacéutica , Estabilidad de Medicamentos , Aceite de Palma , Polvos , Tensoactivos , Factores de Tiempo , AguaRESUMEN
P-glycoprotein (P-gp) is a multidrug transporter that uses energy from ATP hydrolysis to export many structurally dissimilar hydrophobic and amphipathic compounds, including anticancer drugs from cells. Several structural studies on purified P-gp have been reported, but only limited and sometimes conflicting information is available on ligand interactions with the isolated transporter in a dodecyl-maltoside detergent environment. In this report we compared the biochemical properties of P-gp in native membranes, detergent micelles, and when reconstituted in artificial membranes. We found that the modulators zosuquidar, tariquidar, and elacridar stimulated the ATPase activity of purified human or mouse P-gp in a detergent micelle environment. In contrast, these drugs inhibited ATPase activity in native membranes or in proteoliposomes, with IC50 values in the 10-40 nm range. Similarly, a 30-150-fold decrease in the apparent affinity for verapamil and cyclic peptide inhibitor QZ59-SSS was observed in detergent micelles compared with native or artificial membranes. Together, these findings demonstrate that the high-affinity site is inaccessible because of either a conformational change or binding of detergent at the binding site in a detergent micelle environment. The ligands bind to a low-affinity site, resulting in altered modulation of P-gp ATPase activity. We, therefore, recommend studying structural and functional aspects of ligand interactions with purified P-gp and other ATP-binding cassette transporters that transport amphipathic or hydrophobic substrates in a detergent-free native or artificial membrane environment.