RESUMEN
The objective of the study was to investigate the protective effect of Apium graveolens (AP) against di-(2-ethylhexyl) phthalate (DEHP)-induced testes injury in rats. Adult rats were divided into nine groups: (1) control group (no treatment); (2) corn oil (60 µg/kg body weight - bwt); (3) AP (50 µg/kg bwt); (4) 300 mg DEHP/kg bwt; (5) 500 mg DEHP/kg bwt; (6) 1000 mg DEHP/kg bwt; (7) 300 mg DEHP/kg bwt+AP; (8) 500 mg DEHP/kg bwt+AP; and (9) 1000 mg DEHP/kg bwt+AP. Oral administration of treatments was performed daily for 6 weeks. DEHP decreased (p<0.01) body weight, testis weight and serum concentrations of testosterone, cholesterol and total proteins. Moreover, DEHP increased (p<0.001) total antioxidant capacity in the testis and plasma DEHP level. In addition, DEHP decreased mRNA expression of two testicular steroidogenic enzymes: 3ß-hydroxysteroid dehydrogenase and 17ß-hydroxysteroid dehydrogenase. DEHP also caused atrophy, vacuolar degeneration and aspermia of the seminiferous tubules. AP administered concurrently with DEHP effectively alleviated most of the DEHP-induced effects. In conclusion, in male rats, DEHP had adverse effects on the testis including inhibition of androgen production. A concurrent administration of A. graveolens (celery oil) protected the testis against DEHP-induced toxicity.
Asunto(s)
Apium/química , Dietilhexil Ftalato/antagonistas & inhibidores , Disruptores Endocrinos/química , Infertilidad Masculina/prevención & control , Aceites Volátiles/uso terapéutico , Fitoterapia , Testículo/efectos de los fármacos , 17-Hidroxiesteroide Deshidrogenasas/antagonistas & inhibidores , 17-Hidroxiesteroide Deshidrogenasas/genética , 17-Hidroxiesteroide Deshidrogenasas/metabolismo , 3-Hidroxiesteroide Deshidrogenasas/antagonistas & inhibidores , 3-Hidroxiesteroide Deshidrogenasas/genética , 3-Hidroxiesteroide Deshidrogenasas/metabolismo , Andrógenos/efectos adversos , Andrógenos/uso terapéutico , Animales , Antioxidantes/efectos adversos , Antioxidantes/uso terapéutico , Dietilhexil Ftalato/administración & dosificación , Dietilhexil Ftalato/farmacocinética , Dietilhexil Ftalato/toxicidad , Relación Dosis-Respuesta a Droga , Egipto , Disruptores Endocrinos/administración & dosificación , Disruptores Endocrinos/farmacocinética , Disruptores Endocrinos/toxicidad , Etnofarmacología , Frutas/química , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Infertilidad Masculina/inducido químicamente , Infertilidad Masculina/metabolismo , Infertilidad Masculina/patología , Masculino , Medicinas Tradicionales Africanas , Aceites Volátiles/efectos adversos , Tamaño de los Órganos/efectos de los fármacos , Fitoterapia/efectos adversos , Ratas Wistar , Semillas/química , Testículo/metabolismo , Testículo/patologíaRESUMEN
The release behavior of diethylhexyl phthalate (DEHP) from polyvinyl chloride (PVC) tubing, which composes materials in an intravenous administration set (IAS), was investigated using polyoxyethylated hydrogenated castor oil (HCO60) in physiological saline (PS), distilled water for injection (DWI), and ribose, fructose, and glucose (TZ) solutions. The amount of DEHP released increased with increasing HCO60 concentration, and the cumulative amount of DEHP released after 4h increased in the following order: 50% TZAsunto(s)
Aceite de Ricino/análogos & derivados
, Aceite de Ricino/química
, Dietilhexil Ftalato/farmacocinética
, Cloruro de Polivinilo/química
, Cromatografía Líquida de Alta Presión
, Dietilhexil Ftalato/química
, Fructosa/química
, Glucosa/química
, Infusiones Intravenosas/métodos
, Micelas
, Ribosa/química
, Factores de Tiempo
RESUMEN
The administration of 1 g/kg di(2-ethylhexyl)phthalate (DEHP) or 5 mg/kg testosterone for 1 week did not affect the testicular and prostatic gland weights in rats. However, co-administration of DEHP and testosterone induced severe testicular atrophy accompanied by a decrease of zinc concentration in the testis and reduction of the activity of testicular specific lactate dehydrogenase isozyme. These changes were similar to the results of high dose administration of DEHP alone. Values of biological half-life and area under the concentration-time curve (AUC) of mono(2-ethylhexyl)phthalate, the main metabolite of DEHP, in testes after a single co-administration of DEHP (p.o.) and testosterone (i.p.) were higher than those after DEHP administration alone. Results suggest that the co-administration of DEHP and testosterone enhanced the adverse effects of DEHP on testes as the result of changes in pharmacokinetic values of MEHP.