RESUMEN
Lymphocytes and their function in patients with pernicious anaemia (PA) have been studied. E-rosette forming cells (E-RFC) and surface immunoglobulin positive (S-Ig) lymphocytes in peripheral blood, PHA responses of peripheral blood lymphocytes and tuberculin skin test (Mantoux) were studied before and during specific treatment with cyanocobalamin. First in order to test possible alterations in the metabolism of lymphocytes in PA, the effect of culture conditions was studied; the optimal conditions proved to be the same as for normal cells. The serum of PA patients as a supplement for culture medium did not differ from normal. When the patients were in remission, responses in a folate poor thymine free medium (TC 199 special) were significantly greater than those obtained using a folate rich medium (RPMI 1640) (P less than 0.01), possibly indicating activation of the salvage pathway for DNA synthesis and, therefore, increased incorporation of 125IUdR in the folate poor medium. In vitro tests did not indicate any disturbances in the functions of lymphocytes either before or during appropriate treatment as studied in 23 PA patients. However, the tuberculin skin test changed from negative to positive in 5 out of 7 cases tested.
Asunto(s)
Anemia Perniciosa/inmunología , Vitamina B 12/uso terapéutico , Adulto , Anciano , Anemia Perniciosa/complicaciones , Anemia Perniciosa/tratamiento farmacológico , Células Cultivadas , Medios de Cultivo , Disgammaglobulinemia/inmunología , Femenino , Humanos , Deficiencia de IgA , Activación de Linfocitos/efectos de los fármacos , Masculino , Persona de Mediana Edad , Fitohemaglutininas/farmacología , Formación de RosetaRESUMEN
Total IgG4 levels were determined in 27 colostrum and 27 plasma paired samples by using RIA techniques, and total IgG was determined on the same pairs by using radial immunodiffusion. In colostrum, the mean IgG4 level was 4.6 micrograms/ml (0.6 to 19.0), and in the plasma the mean IgG4 level was 170.5 micrograms/ml (30 to 920). IgG averaged 42.3 micrograms/ml (12 to 240) in colostrum and 7980.9 micrograms/ml (3250 to 16,000) in plasma. Of colostral IgG, 15.3% was IgG4, whereas only 3.5% of plasma IgG was IgG4. Specific IgG4 antibodies to beta-lactoglobulin, bovine serum albumin, bermuda grass, and alpha-gliadin were also assayed. In six patients, strong evidence was found for local mammary production of IgG4-specific antibodies.
Asunto(s)
Calostro/inmunología , Inmunoglobulina G/biosíntesis , Alérgenos/inmunología , Animales , Bovinos , Disgammaglobulinemia/inmunología , Femenino , Gliadina/inmunología , Humanos , Deficiencia de IgG , Inmunoglobulina G/análisis , Lactoglobulinas/inmunología , Albúmina Sérica Bovina/inmunología , PorcinosAsunto(s)
Lactancia Materna , Inmunoglobulina A Secretora , Inmunoglobulina A , Adulto , Calostro/inmunología , Disgammaglobulinemia/inmunología , Femenino , Humanos , Inmunidad , Inmunoglobulina A/análisis , Inmunoglobulina A Secretora/análisis , Inmunoglobulina A Secretora/biosíntesis , Recién Nacido , Leche Humana/inmunologíaRESUMEN
Immunoglobulin (Ig) M purified from bovine colostrum was examined by an immunodiffusion analysis with antisecretory IgA serum and was found to be associated with a secretory component. Some of the combined proteins were dissociated if treated with 5 M guanidine-HCl and others were not. Another immunodiffusion analysis of 23 specimens of colostrum led to the finding that certain colostrums were deficient in IgA, even though they contained IgG and IgM.