RESUMEN
BACKGROUND: The common weeds Echinochloa crus-galli L. and Setaria glauca L. were studied for their sensitivity to aqueous extracts or dry biomass of the following cover crops (CCs): Brassicaceae (Sinapis alba, Raphanus sativus var. Oleiformis, Camellina sativa), Fagopyrum esculentum and Guizotia abyssinica. RESULTS: Treating E. crus-galli with aqueous extracts of mixed CCs or individual brassica CC significantly reduced germination. Treating S. glauca with aqueous extracts of C. sativa or G. abyssinicia reduced germination. Aqueous extracts of all CCs significantly reduced radicle length of E. crus-galli and S. glauca, with C. sativa and mixed CCs showing the greatest effect. Aqueous extracts of nearly all CCs delayed start and middle germination of E. crus-galli and S. glauca, with S. alba and R. sativus showing the strongest effects. Aqueous extracts of Brassicaceae leaf and flower significantly reduced germination, coleoptile length, radicle length and seedling biomass of E. crus-galli and S. glauca. Brassicaceae leaves and flowers contained higher phenolics than other tissues. Adding 4 or 8% S. alba and R. sativus dry powder to soil significantly reduced growth of E. crus-galli and S. glauca; even concentrations of 1% measurably slowed growth of E. crus-galli. CONCLUSIONS: Brassicaceae may be allelopathic to S. glauca and E. crus-galli. Aqueous extracts of leaves and flowers showed greater phytotoxic activity than other tissues and also contained more phenolics. Therefore Brassicaceae CCs may be most effective against S. glauca and E. crus-galli if incorporated into soil during their flowering stage. © 2020 Society of Chemical Industry.
Asunto(s)
Asteraceae/química , Brassicaceae/química , Echinochloa/efectos de los fármacos , Fagopyrum/química , Herbicidas/farmacología , Extractos Vegetales/farmacología , Setaria (Planta)/efectos de los fármacos , Alelopatía , Echinochloa/crecimiento & desarrollo , Herbicidas/química , Extractos Vegetales/química , Malezas/efectos de los fármacos , Setaria (Planta)/crecimiento & desarrolloRESUMEN
Diterpenoids are the main secondary metabolites of plants and with a range of biological activities. In the present study, 7 compounds were isolated from the hulls of rice (Oryza sativa L.). Among them, 3 diterpenoids are new namely, 3,20-epoxy-3α-hydroxy- 8,11,13-abietatrie-7-one (1), 4,6-epoxy-3ß-hydroxy-9ß-pimara-7,15-diene (2) and 2-((E)-3- (4-hydroxy-3-methoxyphenyl) allylidene) momilactone A (3). While, 4 terpenoids are known, namely momilactone A (4), momilactone B (5), ent-7-oxo-kaur-15-en-18-oic acid (6) and orizaterpenoid (7). The structures of these diterpenoids were elucidated using 1D and 2D NMR in combination with ESI-MS and HR-EI-MS. Furthermore, all isolated compounds displayed antifungal activities against four crop pathogenic fungi Magnaporthe grisea, Rhizoctonia solani, Blumeria graminearum and Fusarium oxysporum, and phytotoxicity against paddy weed Echinochloa crusgalli. The results suggested that rice could produce plenty of secondary metabolites to defense against weeds and pathogens.
Asunto(s)
Diterpenos/farmacología , Fungicidas Industriales/farmacología , Herbicidas/farmacología , Oryza/química , Semillas/química , Diterpenos/aislamiento & purificación , Echinochloa/efectos de los fármacos , Fungicidas Industriales/aislamiento & purificación , Herbicidas/aislamiento & purificación , Estructura Molecular , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacologíaRESUMEN
In the present study, the essential oil (EO) of Hyptis suaveolens has been explored for the first time for its phytotoxic and cytotoxic activities. The phytotoxic activity was assessed against rice (Oryza sativa) and its major troublesome weed, Echinochloa crus-galli, under laboratory and screenhouse conditions. GC-MS analysis revealed EO to be monoterpenoid (~ 79% monoterpenes) in nature with α-phellandrene (22.8%), α-pinene (10.1%) and limonene (8.5%) as the major chemical constituents. The laboratory bioassay showed a complete growth inhibitory effect of EO (≥ 2â¯mgâ¯mL-1) towards the germination and seedling growth of E. crus-galli. However, the inhibitory effect on rice was much less (~40% inhibition). EO caused visible injury, reduction in chlorophyll content, cell viability and ultimately led to complete wilting of E. crus-galli plants. In addition, EO altered the cell division in the meristematic cells of Allium cepa as depicted by ~63% decrease in mitotic index. EO exposure induced several aberrations at chromosomal (c-mitosis, anaphase bridges, chromosomal breakage, vagrant chromosomes, and sticky chromosomes) and cytological level (cytoplasm destruction, peripheral nuclei, and bi-nucleate cells). The present study concludes that H. suaveolens EO possesses phytotoxic activity due to its mito-depressive activity, and could serve as a natural herbicide under sustainable agricultural practices.
Asunto(s)
Herbicidas , Hyptis/química , Aceites Volátiles/toxicidad , Aceites de Plantas/toxicidad , Monoterpenos Bicíclicos , División Celular , Aberraciones Cromosómicas , Monoterpenos Ciclohexánicos , Echinochloa/efectos de los fármacos , Echinochloa/crecimiento & desarrollo , Germinación/efectos de los fármacos , Herbicidas/química , Herbicidas/toxicidad , Limoneno/análisis , Monoterpenos/análisis , Aceites Volátiles/química , Oryza/efectos de los fármacos , Aceites de Plantas/químicaRESUMEN
QYR301, 1,3-Dimethyl-1H-pyrazole-4-carboxylic acid 4-[2-chloro-3-(3,5-dimethyl-pyrazol-1-ylmethyl)-4-methanesulfonyl-benzoyl]-2,5-dimethyl-2H-pyrazol-3-yl ester, is a novel HPPD-inhibiting herbicide and was evaluated to provide a reference for post-emergence (POST) application under greenhouse and field conditions. The crop safety (180 and 360 g active ingredient (a.i.) ha-1 treatments) experiment revealed that wheat, paddy, garlic and corn were the only four crops without injury at both examined herbicide rates. The weed control efficacy (60 and 120 g a.i. ha-1) experiment showed that QYR301 exhibited high efficacy against many weeds, especially weeds infesting paddy fields. Furthermore, it is interesting that both susceptible and multiple herbicide resistant Echinochloa crus-galli (L.) Beauv. and Echinochloa phyllopogon (Stapf) Koss, two notorious weed species in paddy field, remained susceptible to QYR301. Further crop tolerance results indicated that 20 tested paddy hybrids displayed different levels of tolerance to QYR301, with the japonica paddy hybrids having more tolerance than indica paddy hybrids under greenhouse conditions. Results obtained from field experiments showed that QYR301 POST at 135 to 180 g a.i. ha-1 was recommended to provide satisfactory full-season control of E. crus-galli and Leptochloa chinensis (L.) Nees and to maximize rice yields. These findings indicate that QYR301 possesses great potential for the management of weeds in paddy fields.
Asunto(s)
Productos Agrícolas/crecimiento & desarrollo , Echinochloa/crecimiento & desarrollo , Ésteres/farmacología , Resistencia a los Herbicidas , Herbicidas/farmacología , Malezas/crecimiento & desarrollo , Pirazoles/farmacología , Control de Malezas/métodos , Productos Agrícolas/efectos de los fármacos , Echinochloa/efectos de los fármacos , Ajo/efectos de los fármacos , Ajo/crecimiento & desarrollo , Efecto Invernadero , Oryza/efectos de los fármacos , Oryza/crecimiento & desarrollo , Malezas/efectos de los fármacos , Triticum/efectos de los fármacos , Triticum/crecimiento & desarrollo , Zea mays/efectos de los fármacos , Zea mays/crecimiento & desarrolloRESUMEN
Aglaia odorata Lour. (Meliaceae) was found to have very strong allelopathic activity and a bioherbicide PORGANIC(™) was developed from its leaf extracts. However, the phytotoxic substances causing the strong allelopathic activity of the plants have not yet been determined. Therefore, we investigated allelopathic properties and phytotoxic substances in A. odorata. Aqueous EtOH extracts of A. odorata leaves inhibited root and shoot growth of garden cress (Lepidum sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), timothy (Phleum pratense), ryegrass (Lolium multiflorum), and Echinochloa crus-galli with the extract concentration-dependent manner. The extracts were then purified and a major phytotoxic substance with allelopathic activity was isolated and identified by spectral data as rocaglaol. Rocaglaol inhibited the growth of garden cress and E. crus-galli at concentrations > 0.3 and 0.03 µm, respectively. The concentrations required for 50% inhibition ranged from 0.09 to 2.5 µm. The inhibitory activity of rocaglaol on the weed species, E. crus-galli, was much greater than that of abscisic acid. These results suggest that rocaglaol may be a major contributor to the allelopathic effect of A. odorata and bioherbicide PORGANIC(™) .
Asunto(s)
Aglaia/química , Benzofuranos/farmacología , Echinochloa/efectos de los fármacos , Lepidium sativum/efectos de los fármacos , Extractos Vegetales/química , Hojas de la Planta/química , Benzofuranos/química , Benzofuranos/aislamiento & purificación , Relación Dosis-Respuesta a Droga , Echinochloa/crecimiento & desarrollo , Lepidium sativum/crecimiento & desarrollo , Conformación MolecularRESUMEN
As a continuous effort to find new natural products with potential herbicide activity, flavonoids acacetin (1), chrysin (2) and 4',7-dimethylnarangenin (3) were isolated from a propolis sample collected in the rural area of Mexico City and their effects on the photosynthesis light reactions and on the growth of Lolium perenne, Echinochloa crus-galli and Physalis ixocarpa seedlings were investigated. Acacetin (1) acted as an uncoupler by enhancing the electron transport under basal and phosphorylating conditions and the Mg(2+)-ATPase. Chrysin (2) at low concentrations behaved as an uncoupler and at concentrations up to 100 µM its behavior was as a Hill reaction inhibitor. Finally, 4',7-dimethylnarangenin (3) in a concentration-dependent manner behaved as a Hill reaction inhibitor. Flavonoids 2 and 3 inhibited the uncoupled photosystem II reaction measured from water to 2,5-dichloro-1,4-benzoquinone (DCBQ), and they did not inhibit the uncoupled partial reactions measured from water to sodium silicomolybdate (SiMo) and from diphenylcarbazide (DPC) to diclorophenol indophenol (DCPIP). These results indicated that chrysin and 4',7-dimethylnarangenin inhibited the acceptor side of PS II. The results were corroborated with fluorescence of chlorophyll a measurements. Flavonoids also showed activity on the growth of seedlings of Lolium perenne and Echinochloa crus-galli.
Asunto(s)
Flavonoides/farmacología , Fotosíntesis/efectos de los fármacos , Própolis/química , Plantones/efectos de los fármacos , Arginina/análogos & derivados , Arginina/química , Arginina/farmacología , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos/métodos , Echinochloa/efectos de los fármacos , Echinochloa/crecimiento & desarrollo , Flavonas/química , Flavonas/farmacología , Flavonoides/química , Flavonoides/aislamiento & purificación , Herbicidas/química , Herbicidas/farmacología , Lolium/efectos de los fármacos , Lolium/crecimiento & desarrollo , Espectroscopía de Resonancia Magnética , México , Complejo de Proteína del Fotosistema II/antagonistas & inhibidores , Complejo de Proteína del Fotosistema II/metabolismo , Physalis/efectos de los fármacos , Physalis/crecimiento & desarrollo , Plantones/crecimiento & desarrollo , Spinacia oleracea/efectos de los fármacosRESUMEN
Hyptis suaveolens (Lamiaceae) is an exotic invasive plant in many countries. Earlier studies reported that the aqueous, methanol, and aqueous methanol extract of H. suaveolens and its residues have phytotoxic properties. However, to date, the phytotoxic substances of this plant have not been reported. Therefore, the objectives of this study were isolation and identification of phytotoxic substances of H. suaveolens. Aqueous methanol extract of this plant was purified by several chromatographic runs through bioassay guided fractionation using garden cress (Lepidium sativum) as a test plant. Final purification of a phytotoxic substance was achieved by reverse phase HPLC and characterized as 14α-hydroxy-13ß-abiet-8-en-18-oic acid (suaveolic acid) by high-resolution ESI-MS, (1)H-,(13)C-NMR, CD, and specific rotation. Suaveolic acid inhibited the shoot growth of garden cress, lettuce (Lactuca sativa), Italian ryegrass (Lolium multiflorum), and barnyard grass (Echinochloa crus-galli) at concentrations greater than 30 µM. Root growth of all but lettuce was also inhibited at concentrations greater than 30 µM. The inhibitory activities were concentration dependent. Concentrations required for 50% growth inhibition of suaveolic acid for those test plant species were ranged from 76 to 1155 µM. Therefore, suaveolic acid is phytotoxic and may be responsible for the phytotoxicity of H. suaveolens plant extracts.
Asunto(s)
Abietanos/toxicidad , Hyptis/química , Raíces de Plantas/efectos de los fármacos , Plantas Tóxicas/química , Toxinas Biológicas/toxicidad , Abietanos/aislamiento & purificación , Echinochloa/efectos de los fármacos , Echinochloa/crecimiento & desarrollo , Hyptis/fisiología , Concentración 50 Inhibidora , Lepidium sativum/efectos de los fármacos , Lepidium sativum/crecimiento & desarrollo , Lactuca/efectos de los fármacos , Lactuca/crecimiento & desarrollo , Lolium/efectos de los fármacos , Lolium/crecimiento & desarrollo , Metanol , Extractos Vegetales/química , Raíces de Plantas/crecimiento & desarrollo , Plantas Tóxicas/fisiología , Solventes , Toxinas Biológicas/aislamiento & purificación , AguaRESUMEN
Phytotoxic activity of Ocimum tenuiflorum (Lamiaceae) plant extracts was investigated against the germination and seedling growth of cress (Lepidium sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), Italian ryegrass (Lolium multiflorum), barnyard grass (Echinochloa crus-galli), and timothy (Phleum pratense) at four different concentrations. The plant extracts at concentrations greater than 30 mg dry weight equivalent extract mL(-1) reduced significantly the total germination percent (GP), germination index (GI), germination energy (GE), speed of emergence (SE), seedling vigour index (SVI), and coefficient of the rate of germination (CRG) of all test species except barnyard grass and GP of lettuce. In contrast, time required for 50% germination (T 50) and mean germination time (MGT) were increased at the same or higher than this concentration. The increasing trend of T 50 and MGT and the decreasing trend of other indices indicated a significant inhibition or delay of germination of the test species by O. tenuiflorum plant extracts and vice versa. In addition, the shoot and root growth of all test species were significantly inhibited by the extracts at concentrations greater than 10 mg dry weight equivalent extract mL(-1). The I 50 values for shoot and root growth were ranged from 26 to 104 mg dry weight equivalent extract mL(-1). Seedling growth was more sensitive to the extracts compared to seed germination. Results of this study suggest that O. tenuiflorum plant extracts have phytotoxic properties and thus contain phytotoxic substances. Isolation and characterization of those substances from this plant may act as a tool for new natural, biodegradable herbicide development to control weeds.
Asunto(s)
Germinación/efectos de los fármacos , Ocimum/química , Extractos Vegetales/toxicidad , Plantones/efectos de los fármacos , Brassicaceae/efectos de los fármacos , Brassicaceae/crecimiento & desarrollo , Echinochloa/efectos de los fármacos , Echinochloa/crecimiento & desarrollo , Lactuca/efectos de los fármacos , Lactuca/crecimiento & desarrollo , Lolium/efectos de los fármacos , Lolium/crecimiento & desarrolloRESUMEN
Leucas aspera (Lamiaceae), an aromatic herbaceous plant, is well known for many medicinal properties and a number of bioactive compounds against animal cells have been isolated. However, phytotoxic substances from L. aspera have not yet been documented in the literature. Therefore, current research was conducted to explore the phytotoxic properties and substances in L. aspera. Aqueous methanol extracts of L. aspera inhibited the germination and growth of garden cress (Lepidum sativum) and barnyard grass (Echinochloa crus-galli), and the inhibitory activities were concentration dependent. These results suggest that the plant may have phytotoxic substances. The extracts were then purified by several chromatographic runs. The final purification was achieved by reversed-phase HPLC to give an equilibrium (or inseparable) 3:2 mixture of two labdane type diterpenes (compounds 1 and 2). These compounds were characterized as (rel 5S,6R,8R,9R,10S,13S,15S,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (1) and (rel 5S,6R,8R,9R,10S,13S,15R,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (2) by spectroscopic analyses. A mixture of the two compounds inhibits the germination and seedling growth of garden cress and barnyard grass at concentrations greater than 30 and 3 µM, respectively. The concentration required for 50% growth inhibition (I50) of the test species ranges from 31 to 80 µM, which suggests that the mixture of these compounds, are responsible for the phytotoxic activity of L. aspera plant extract.
Asunto(s)
Lamiaceae/química , Extractos Vegetales/química , Extractos Vegetales/farmacología , Diterpenos/química , Diterpenos/farmacología , Echinochloa/efectos de los fármacos , Germinación/efectos de los fármacos , Lepidium sativum/efectos de los fármacosRESUMEN
Aqueous methanol extracts of the traditional rice (Oryza sativa) variety Awaakamai, which is known to have the greatest allelopathic activity among Japanese traditional rice varieties, inhibited the growth of roots and shoots of cress (Lepidium sativum), lettuce (Lactuca sativa), timothy (Phleum pratense), Digitaria sanguinalis, Lolium multiflorum and Echinochloa crus-galli. Increasing the extract concentration increased the inhibition, suggesting that the extract of Awaakamai contains growth inhibitory substances. The extract of Awaakamai was purified and two main growth inhibitory substances were isolated and determined by spectral data as blumenol A and grasshopper ketone. Blumenol A and grasshopper ketone, respectively, inhibited the growth of cress shoots and roots at concentrations greater than 10 and 30 µmol/L. The concentrations required for 50% growth inhibition on cress roots and shoots were 84 and 27 µmol/L, respectively, for blumenol A, and 185 and 76 µmol/L, respectively, for grasshopper ketone. These results suggest that blumenol A and grasshopper ketone may contribute to the growth inhibitory effect of Awaakamai and may play an important role in the allelopathy of Awaakamai.
Asunto(s)
Cetonas/aislamiento & purificación , Oryza/química , Extractos Vegetales/química , Reguladores del Crecimiento de las Plantas/aislamiento & purificación , Alcadienos/química , Alcadienos/aislamiento & purificación , Alcadienos/farmacología , Bioensayo , Ciclohexanoles/química , Ciclohexanoles/aislamiento & purificación , Ciclohexanoles/farmacología , Ciclohexanonas/química , Ciclohexanonas/aislamiento & purificación , Ciclohexanonas/farmacología , Relación Dosis-Respuesta a Droga , Echinochloa/efectos de los fármacos , Echinochloa/crecimiento & desarrollo , Cetonas/química , Cetonas/farmacología , Lepidium sativum/efectos de los fármacos , Lepidium sativum/crecimiento & desarrollo , Oryza/clasificación , Extractos Vegetales/aislamiento & purificación , Reguladores del Crecimiento de las Plantas/farmacología , Hojas de la Planta/química , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/crecimiento & desarrollo , Tallos de la Planta/química , Control de MalezasRESUMEN
We have isolated five endophytic fungi from the roots of Capsicum annuum, Cucumis sativus and Glycine max. The culture filtrates (CF) of these endophytes were screened on dwarf mutant rice (Waito-C) and normal rice (Dongjin-byeo). Endophyte CAC-1A significantly inhibited the growth of Waito-C and Dongjin-byeo. Endophyte CAC-1A was identified as Paraconiothyrium sp. by sequencing the ITS rDNA region and phylogenetic analysis. The ethyl acetate fraction of Paraconiothyrium sp. suppressed the germination of Lactuca sativa and Echinochloa crus-galli seeds. The ethyl acetate fraction of the endophyte was subjected to bioassay-guided isolation and we obtained the phytotoxic compound ascotoxin (1) which was characterized through NMR and GC/MS techniques. Ascotoxin revealed 100% inhibitory effects on seed germination of Echinochloa crus-galli. Compound (1) was isolated for the first time from Paraconiothyrium sp.
Asunto(s)
Brefeldino A/metabolismo , Endófitos/aislamiento & purificación , Herbicidas/metabolismo , Extractos Vegetales/metabolismo , Saccharomycetales/aislamiento & purificación , Brefeldino A/farmacología , Echinochloa/efectos de los fármacos , Echinochloa/fisiología , Endófitos/metabolismo , Germinación/efectos de los fármacos , Herbicidas/farmacología , Lactuca/efectos de los fármacos , Lactuca/fisiología , Oryza/efectos de los fármacos , Oryza/fisiología , Filogenia , Extractos Vegetales/farmacología , Raíces de Plantas/microbiología , ARN Ribosómico 18S/genética , Saccharomycetales/genética , Saccharomycetales/metabolismo , Análisis de Secuencia de ADNRESUMEN
Here, we investigated chemical-mediated interaction between crop and weeds. Allelopathic activity of rice seedlings exhibited 5.3-6.3-fold increases when rice and barnyard grass seedlings were grown together, where there may be the competitive interference between rice and barnyard grass for nutrients. Barnyard grass is one of the most noxious weeds in rice cultivation. The momilactone B concentration in rice seedlings incubated with barnyard grass seedlings was 6.9-fold greater than that in rice seedlings incubated independently. Low nutrient growth conditions also increased allelopathic activity and momilactone B concentrations in rice seedlings. However, the increases in the low nutrient-induced allelopathic activity and momilactone B concentration were much lower than those in barnyard grass-induced allelopathic activity and momilactone B concentration. Root exudates of barnyard grass seedlings increased allelopathic activity and momilactone B concentration in rice seedlings at concentrations greater than 30 mg/L of the root exudates, and increasing the exudate concentration increased the activity and momilactone B concentration. Therefore, barnyard grass-induced allelopathic activity of rice seedlings may be caused not only by nutrient competition between two species, but also by components in barnyard grass root exudates. As momilactone B shows strong allelopathic activities, barnyard grass-induced allelopathic activity of rice may be due to the increased concentration of momilactone B in rice seedlings. The present research suggests that rice may respond to the presence of neighboring barnyard grass by sensing the components in barnyard grass root exudates and increasing allelopathic activity by production of elevated concentration of momilactone B. Thus, rice allelopathy may be one of the inducible defense mechanisms by chemical-mediated plant interaction between rice and barnyard grass, and the induced-allelopathy may provide a competitive advantage for rice through suppression of the growth of barnyard grass.
Asunto(s)
Diterpenos/metabolismo , Echinochloa/efectos de los fármacos , Lactonas/metabolismo , Oryza/metabolismo , Feromonas/metabolismo , Extractos Vegetales/farmacología , Alelos , Diterpenos/análisis , Diterpenos/farmacología , Echinochloa/química , Echinochloa/crecimiento & desarrollo , Lactonas/análisis , Lactonas/farmacología , Oryza/química , Oryza/efectos de los fármacos , Oryza/crecimiento & desarrollo , Feromonas/farmacología , Extractos Vegetales/química , Extractos Vegetales/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/crecimiento & desarrollo , Malezas , Plantones/química , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Plantones/metabolismo , Transducción de Señal , Factores de TiempoRESUMEN
Heavy metal uptake and growth by Echinochloa crus-galli were investigated to determine if the use of root exudates enhanced phytoextraction. E. crus-galli were planted in soils contaminated with 600 mg kg(-1) Pb, 40 mg kg(-1) Cd and 100 mg kg(-1) Cu. E. crus-galli were then cultivated with and without root exudates from Belamcanda chinensis for 4 weeks. The growth of E. crus-galli in metal-contaminated soils that contained root exudates showed increased roots and shoots when compared to E. crus-galli grown without root exudates (p<0.05). In addition, the accumulation of metal in E. crus-galli that was cultivated with the root exudates was two- to fourfold higher than in plants that were cultivated without the root exudates. The exchangeable soil fraction in the rhizosphere of E. crus-galli grown with root exudates was greater than when E. crus-galli was grown without root exudates. Finally, the BCF and TF values of Cd, Cu and Pb were greater when the root exudates were added (p<0.05). Taken together, these results indicate that root exudates can be used as a natural chelating agent to enhance phytoextraction.
Asunto(s)
Echinochloa , Restauración y Remediación Ambiental/métodos , Iridaceae/química , Metales Pesados/metabolismo , Extractos Vegetales/farmacología , Contaminantes del Suelo/metabolismo , Quelantes/farmacología , Echinochloa/efectos de los fármacos , Echinochloa/metabolismo , Micorrizas , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , Brotes de la Planta/crecimiento & desarrolloRESUMEN
Aqueous methanol extracts of red pine (Pinus densiflora) needles inhibited the growth of roots and shoots of cress (Lepidium sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), ryegrass (Lolium multiflorum), timothy (Pheleum pratense), Digitaria sanguinalis and Echinochloa crus-galli. Increasing the extract concentration increased inhibition, suggesting that the pine needles may have growth inhibitory substances and possess allelopathic potential. The aqueous methanol extract of the pine needles was purified, and a main inhibitory substance was isolated and determined by spectral data as 9alpha,13beta-epidioxyabeit-8(14)en-18-oic acid. This substance inhibited root and shoot growth of cress and Echinochloa crus-galli seedlings at concentrations greater than 0.1 mM. The endogenous concentration of the substance was 0.13 mmol/kg pine needle. These results suggest that 9alpha,13beta-epidioxyabeit-8(14)en-18-oic acid may contribute to the growth inhibitory effect of the pine needles and may play an important role in the allelopathy of red pine.
Asunto(s)
Feromonas/aislamiento & purificación , Pinus/química , Hojas de la Planta/química , Echinochloa/efectos de los fármacos , Echinochloa/crecimiento & desarrollo , Lepidium sativum/efectos de los fármacos , Lepidium sativum/crecimiento & desarrollo , Feromonas/química , Feromonas/farmacología , Extractos Vegetales , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/crecimiento & desarrollo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrolloRESUMEN
Methyl jasmonate (MeJA) and methyl salicylate (MeSA) are important signaling molecules that induce plant defense against insect herbivores and microbial pathogens. We tested the hypothesis that allelopathy is an inducible defense mechanism, and that the JA and SA signaling pathways may activate allelochemicals release. Exogenous application of MeJA and MeSA to rice (Oryza sativa L.) enhanced rice allelopathic potential and led to accumulation of phenolics, an increase in enzymatic activities, and gene transcription of phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H), two key enzymes in the phenylpropanoid pathway. Aqueous extracts of the leaves of rice IAC165, a putative allelopathic variety, treated with MeSA (5 mM) or MeJA (0.05 mM), showed increased inhibitory effects (25 and 21%, respectively) on root growth of barnyardgrass (Echinochloa crus-galli L.), and increased inhibitory effects (18 and 23%, respectively) on shoot growth. Aqueous extracts from leaves of Huajingxian 1 rice, a putative nonallelopathic variety treated with MeJA and MeSA, caused 63 and 24% inhibition of root growth in barnyardgrass seedlings. The root exudates of both IAC165 and Huajingxian 1 plants treated with MeJA and MeSA for 48 hr also showed significant increases in their inhibitory effects on root growth of barnyardgrass seedlings. At the four-leaf stage, levels of 3,4-hydroxybenzoic acid, vanillic acid, coumaric acid, and ferulic acid that accumulated in the leaves were 5.3-, 31.3-, 2.2-, and 1.7-fold higher in response to MeJA exposure, and 3.3-, 13.1-, 2.0-, and 2.2-fold higher in response to MeSA. Treatments of MeSA and MeJA enhanced the PAL activity in the rice leaves up to 52.3 and 80.1%, respectively, whereas C4H activity was increased by 40.2 and 67%. Gene transcription of PAL and C4H in rice leaves significantly increased after the plants were subjected to treatment with MeJA and MeSA. These results suggest that allelopathy may be an active defense mechanism, and that plant signaling compounds are potentially valuable in its regulation.
Asunto(s)
Acetatos/farmacología , Ciclopentanos/farmacología , Oryza/efectos de los fármacos , Feromonas/metabolismo , Salicilatos/farmacología , Ácidos Carboxílicos/metabolismo , Echinochloa/efectos de los fármacos , Echinochloa/crecimiento & desarrollo , Oryza/metabolismo , Oxilipinas , Fenilanina Amoníaco-Liasa/metabolismo , Extractos Vegetales/farmacología , Hojas de la Planta/química , Raíces de Plantas/química , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/crecimiento & desarrollo , Tallos de la Planta/química , Transcinamato 4-Monooxigenasa/metabolismoRESUMEN
Rice is one of the most interesting crops in the world from both the social and the economic point of views. The monoculture practices along with the heavy use of herbicides are characteristic of modern agriculture and are inducing the appearance of tolerant and/or herbicide resistant weed biotypes. This is the case the world's main weed of rice barnyardgrass (Echinochloa crus-galli). Alternative strategies for weed suppression consist of the use of chemicals from rice due to necessity of obtaining new herbicides with new modes of action that could prevent resistance phenomena. In order to carry out a study that guides to the isolation of the most active compounds from rice, different extracts were achieved, and their activities evaluated. So, all the plant material was divided into three parts: fresh plant, dried plant, and fresh plant from Pluviotron. The aerial part was separated from roots in all cases and extracted in water, in organic solvents as well as with the Pluviotron device. The activity of the 12 extracts obtained was evaluated using a generalist bioassay, wheat etiolated coleoptiles bioassay, and a phytotoxic bioassay on barnyardgrass as target species. The bioactive extracts were fractionated and 15 compounds were isolated and identified by spectroscopic methods. Eight of these compounds were isolated for the first time in Oryza sativa. The most phytotoxic compounds on E. crus-galli were ergosterol peroxide and 7-oxo-stigmasterol. In the case of ergosterol peroxide the activity was higher than the commercial herbicide Logran. This is the first report of potential allelopathic activity of steroids on weeds based on their phytotoxicity.
Asunto(s)
Oryza/química , Esteroides/farmacología , Cotiledón/citología , Cotiledón/efectos de los fármacos , Echinochloa/efectos de los fármacos , Echinochloa/crecimiento & desarrollo , Ergosterol/análogos & derivados , Ergosterol/farmacología , Herbicidas/química , Herbicidas/farmacología , Oryza/citología , Extractos Vegetales/farmacología , Esteroides/química , Esteroles/farmacología , Triticum/citología , Triticum/efectos de los fármacosRESUMEN
Bioassay-directed fractionation of a CH(2)Cl(2)-MeOH (1:1) extract of the aerial parts of Flourensia cernua led to the isolation of three phytotoxic compounds, namely, dehydroflourensic acid (1), flourensadiol (2) and methyl orsellinate (3). Dehydroflourensic acid is a new natural product whose structure was established by spectral means. In addition, the known flavonoid ermanin and seven hitherto unknown gamma-lactones were obtained, these being tetracosan-4-olide, pentacosan-4-olide, hexacosan-4-olide, heptacosan-4-olide, octacosan-4-olide, nonacosan-4-olide, and triacontan-4-olide. Compounds 1-3 caused significant inhibition of radicle growth of Amaranthus hypochondriacus and Echinochloa crus-galli, interacted with bovine-brain calmodulin and inhibited the activation of the calmodulin-dependent enzyme cAMP phosphodiesterase.