RESUMEN
Aqueous extracts of green and black tea are shown to quench reactive oxygen species such as singlet oxygen, superoxide and hydroxyl radicals, prevent the oxidative cross-linking of test proteins and inhibit single strand breakage of DNA in whole cells. They are also seen to be able to counteract the oxidative insult mounted by cigarette smoke. In rats in which cataract was induced by subcutaneous injection of selenite, administration of green or black tea extracts led to a retardation of the progression of lens opacity, suggesting the potential cataracto-static ability of tea.
Asunto(s)
Antioxidantes/uso terapéutico , Catarata/prevención & control , Fitoterapia , Té/uso terapéutico , Animales , Ensayo Cometa , Espectroscopía de Resonancia por Spin del Electrón/métodos , Electroforesis en Gel de Poliacrilamida , Escherichia coli/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Estrés Oxidativo/efectos de los fármacos , Plantas Tóxicas , Pseudomonas aeruginosa/efectos de los fármacos , Ratas , Ratas Wistar , Especies Reactivas de Oxígeno/fisiología , Humo , Espectrometría de Fluorescencia/métodos , Staphylococcus aureus/efectos de los fármacos , NicotianaRESUMEN
Oxidative stress is involved in the aetiology of defective embryo development. Reactive oxygen species (ROS) may originate from embryo metabolism and/or embryo surroundings. Embryo metabolism generates ROS via several enzymatic mechanisms. The relative contribution of each source seems different depending on the species, the stage of development, and the culture conditions. Several exogenous factors and culture conditions can enhance the production of ROS by embryos. ROS can alter most types of cellular molecules, and also induce development block and retardation. Multiple mechanisms of embryo protection against ROS exist, and these have complementary actions. External protection, present in follicular and tubal fluids, mainly comprises non-enzymatic antioxidants such as hypotaurine, taurine and ascorbic acid. Internal protection mainly comprises antioxidant enzymes: superoxide dismutase, glutathione peroxidase and gamma-glutamylcysteine synthetase. Transcripts encoding for these enzymes are present in the oocyte, embryo and oviduct. It may be important that these transcripts are stored during oocyte maturation in order to allow the embryo to acquire the aptitude to develop. It is now common to add antioxidant compounds to culture media. Nevertheless, maintaining the pro-oxidant-antioxidant equilibrium in embryos through such supplementation is a complex problem. Further studies are necessary to limit oxidative stress during embryo culture.
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Blastocisto/fisiología , Estrés Oxidativo/fisiología , Especies Reactivas de Oxígeno/fisiología , Animales , Antioxidantes/metabolismo , Blastocisto/enzimología , Desarrollo Embrionario y Fetal/fisiología , Femenino , Fertilización In Vitro , Humanos , Masculino , Embarazo , Especies Reactivas de Oxígeno/metabolismoRESUMEN
For the first time in history, populations in affluent countries may concomitantly indulge in rich food and physical idleness. Various combinations of obesity, diabetes, and hypertriglyceridemia, with insulin resistance as the common feature, cause hepatic steatosis, which can trigger necroinflammation and fibrosis. Patients with "primary" steatohepatitis exhibit ultrastructural mitochondrial lesions, decreased activity of respiratory chain complexes, and have impaired ability to resynthesize ATP after a fructose challenge. Mitochondria play a major role in fat oxidation and energy production but also leak reactive oxygen species (ROS) and are the main cellular source of ROS. In patients with steatosis, mitochondrial ROS may oxidize hepatic fat deposits, as suggested in animal models. Lipid peroxidation products impair the flow of electrons along the respiratory chain, which may cause overreduction of respiratory chain components, further increasing mitochondrial ROS formation and lipid peroxidation. Another vicious circle could involve ROS-induced depletion of antioxidants, impairing ROS inactivation. Blood vitamin E is decreased in some obese children with steatohepatitis, and serum transaminases improve after vitamin E supplementation. Steatohepatitis is also caused by alcohol abuse, drugs, and other causes. In "secondary" steatohepatitis, mitochondrial ROS formation is further increased as the causative disease itself directly increases ROS or first impairs respiration, which secondarily increases mitochondrial ROS formation. This "second hit" could cause more lipid peroxidation, cytokine induction, Fas ligand induction, and fibrogenesis than in primary steatohepatitis.
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Hígado Graso/etiología , Mitocondrias/fisiología , Especies Reactivas de Oxígeno/fisiología , Envejecimiento/metabolismo , Animales , Enfermedad Crónica , Metabolismo Energético , Ácidos Grasos/metabolismo , Hígado Graso/metabolismo , Glucosa/metabolismo , Humanos , Peroxidación de Lípido , Ratones , Oxidación-ReducciónRESUMEN
The generation of reactive oxygen species (free radicals) is an important factor in the development and maintenance of rheumatoid arthritis in humans and animal models. One source of free radicals is nitric oxide produced within the synoviocytes and chondrocytes and giving rise to the highly toxic radical peroxynitrite. Several cytokines, including tumour necrosis factor-alpha (TNFalpha) are involved in the formation of free radicals, partly by increasing the activity of nitric oxide synthase. Indeed, nitric oxide may mediate some of the deleterious effects of cytokines on bone resorption. Aspirin, tetracyclines, steroids and methotrexate can suppress nitric oxide synthase. Dietary antioxidants include ascorbate and the tocopherols and beneficial effects of high doses have been reported especially in osteoarthritis. There is also evidence for beneficial effects of beta-carotene and selenium, the latter being a component of the antioxidant enzyme glutathione peroxidase. The polyunsaturated fatty acids (PUFA) include the n-3 compounds, some of which are precursors of eicosanoid synthesis, and the n-6 group which can increase formation of the pro-inflammatory cytokines TNFalpha and interleukin-6, and of reactive oxygen species. Some prostaglandins, however, suppress cytokine formation, so that n-3 PUFA often oppose the inflammatory effects of some n-6-PUFA. gamma-linolenic acid (GLA) is a precursor of prostaglandin E1, a fact which may account for its reported ability to ameliorate arthritic symptoms. Fish oil supplements, rich in n-3 PUFA such as eicosapentaenoic acid have been claimed as beneficial in rheumatoid arthritis, possibly by suppression of the immune system and its cytokine repertoire. Some other oils of marine origin (e.g. from the green-lipped mussel) and a range of vegetable oils (e.g. olive oil and evening primrose oil) have indirect anti-inflammatory actions, probably mediated via prostaglandin E1. Overall, there is a growing scientific rationale for the use of dietary supplements as adjuncts in the treatment of inflammatory disorders such as rheumatoid arthritis and osteoarthritis.
Asunto(s)
Antioxidantes/uso terapéutico , Artritis Reumatoide/dietoterapia , Artritis Reumatoide/tratamiento farmacológico , Suplementos Dietéticos , Ácidos Grasos Insaturados/uso terapéutico , Artritis Reumatoide/fisiopatología , Citocinas/fisiología , Aceites de Pescado/uso terapéutico , Humanos , Osteoartritis/dietoterapia , Osteoartritis/tratamiento farmacológico , Especies Reactivas de Oxígeno/fisiología , Vitaminas/uso terapéuticoRESUMEN
Epidemiologic data suggest that the environment is responsible for most prostate cancers (PCA). One major mechanism by which the environment can influence carcinogenesis is oxidative damage. This refers to the generation of reactive oxygen species (ROS) that then damage important biomolecules, including DNA, protein, and lipids. Experimental observations suggest that oxidative damage is associated with PCA. These include: a) the association of PCA and dietary fat consumption (a major substrate for oxidative stress), b) oxidative biomarker data (suggesting increased oxidative stress among patients with PCA), c) ubiquitous defects in the glutathione-s-transferase pi pathway (a major endogenous antioxidant mechanism), and d) evidence that androgens (an important promoter of PCA growth) work in part via generation of ROS. Perhaps the best indirect evidence for oxidative stress comes from randomized double-blind prevention trials of antioxidants. Vitamin E and selenium have both been shown to reduce prostate cancer incidence. Although PCA prevention was not the primary endpoint of these studies, the statistical likelihood that both would prove beneficial by chance alone is 1 in 400. These data suggest that antioxidants may be beneficial in preventing PCA. Further research including randomized trials is warranted.
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Anticarcinógenos/uso terapéutico , Antioxidantes/uso terapéutico , Estrés Oxidativo/fisiología , Neoplasias de la Próstata/prevención & control , Andrógenos/fisiología , Carotenoides/uso terapéutico , Grasas de la Dieta/efectos adversos , Predicción , Humanos , Licopeno , Masculino , Neoplasias de la Próstata/etiología , Especies Reactivas de Oxígeno/fisiología , Selenio/uso terapéutico , Vitamina E/uso terapéuticoRESUMEN
BACKGROUND: Skeletal injury and associated ischemia and inflammation induce the generation of pro-oxidants such as peroxynitrite (ONOO-), which has been demonstrated to induce apoptosis in several cell lines. Fibroblast growth factor (FGF-1) is important for coordinating osteogenesis and angiogenesis of osseous repair. In vitro studies were performed examining the effect of FGF-1 on human osteoblast progenitor stromal stem (HSS) cell proliferation, differentiation, and response to ONOO-. METHODS: HSS cells were isolated and growth kinetics determined in the presence and absence of FGF-1. The effect of FGF-1 on HSS cell expression of osteoblast-specific osteopontin and osteocalcin mRNA and protein was examined by reverse transcriptase polymerase chain reaction and Western blot techniques. To determine the sensitivity of HSS cells to ONOO- in the absence and presence of FGF-1 pretreatment, cells were exposed to varying concentrations of the oxidant and examined for cell death using quantitative fluorescence staining with fluorescein diacetate and propidium diacetate. RESULTS: Treatment of HSS cells with FGF-1 significantly enhanced cellular growth rates by 5 days (4.6 x 105 cells/mL vs. 3.1 x 105 cells/mL) and induced expression of both osteopontin and osteocalcin mRNA and protein. Exposure of HSS cells to ONOO- resulted in a dose- and time-dependent delayed cell death that was more characteristic of apoptosis than necrosis. Pretreatment of HSS cells with FGF-1 prevented ONOO- mediated apoptosis. CONCLUSION: In vitro, treatment of HSS cells with FGF-1 stimulates cell growth and induces expression of differentiation markers specific to osteoblasts. FGF-1 treatment renders osteoblast precursors resistant to the cytotoxic effects of ONOO-. These results suggest that FGF-1 promotes the progression of bone repair mechanisms by increasing the population of osteoblasts and imparting protection to the cell line from the hostile inflammatory environment.
Asunto(s)
Citotoxicidad Inmunológica/fisiología , Factor 1 de Crecimiento de Fibroblastos/fisiología , Factor 1 de Crecimiento de Fibroblastos/uso terapéutico , Curación de Fractura/efectos de los fármacos , Curación de Fractura/inmunología , Fracturas Óseas/tratamiento farmacológico , Fracturas Óseas/inmunología , Neovascularización Fisiológica/fisiología , Nitratos/fisiología , Osteogénesis/fisiología , Oxidantes/fisiología , Especies Reactivas de Oxígeno/fisiología , Células Madre/fisiología , Células del Estroma/fisiología , Apoptosis/fisiología , Diferenciación Celular/fisiología , División Celular/fisiología , Células Cultivadas , Evaluación Preclínica de Medicamentos , Humanos , Inflamación , Osteocalcina , Osteopontina , SialoglicoproteínasRESUMEN
Spermatogenesis is inhibited in Balb/C mice as a result of oxyradical insult. However, mammalian spermatocytes and synaptonemal complexes retain their structure and function after oxyradical insult due to protection afforded by the antioxidant vitamin E. Control groups were compared with experimental groups which were fed various vitamin E-deficient diets and subjected to varying times in an humidified 100% oxygen (hyperoxia) chamber. Measurements were made of sex body volume (SBV), nuclear envelope aberrations (NEA), and synaptonemal complex structure in spermatocytes during pachytene of meiosis prophase I. Changes in the volume of the sex body were positively correlated with increased oxyradical insult. The structure of the synaptonemal complex was not altered in any of the experimental groups which is a significant observation. It is suggested that vitamin E affords antioxidant protection and inhibits the alteration of membranes and sex chromosomes in mice during meiosis.
Asunto(s)
Especies Reactivas de Oxígeno/fisiología , Espermatozoides/efectos de los fármacos , Espermatozoides/ultraestructura , Complejo Sinaptonémico/efectos de los fármacos , Vitamina E/farmacología , Animales , Antioxidantes/farmacología , Núcleo Celular/efectos de los fármacos , Núcleo Celular/ultraestructura , Suplementos Dietéticos , Masculino , Ratones , Ratones Endogámicos BALB C , Membrana Nuclear/efectos de los fármacos , Membrana Nuclear/ultraestructura , Oxígeno/administración & dosificación , Oxígeno/efectos adversos , Superóxidos/farmacología , Tasa de SupervivenciaRESUMEN
Apoptosis is a form of programmed cell death that occurs in neurons during development of the nervous system and may also be a prominent form of neuronal death in chronic neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Recent findings also implicate apoptosis in neuronal degeneration after ischemic brain injury in animal models of stroke. Activation of both apoptotic and antiapoptotic signaling cascades occurs in neurons in animal and cell culture models of stroke. Apoptotic cascades involve: increased levels of intracellular oxyradicals and calcium; induction of expression of proteins such as Par-4 (prostate apoptosis response-4), which act by promoting mitochondrial dysfunction and suppressing antiapoptotic mechanisms; mitochondrial membrane depolarization, calcium uptake, and release of factors (e.g., cytochrome c) that ultimately induce nuclear DNA condensation and fragmentation; activation of cysteine proteases of the caspase family; activation of transcription factors such as AP-1 that may induce expression of "killer genes." Antiapoptotic signaling pathways are activated by neurotrophic factors, certain cytokines, and increases in oxidative and metabolic stress. Such protective pathways include: activation of the transcription factors (e.g., nuclear factor-kappa B, NF-kappa B) that induce expression of stress proteins, antioxidant enzymes, and calcium-regulating proteins; phosphorylation-mediated modulation of ion channels and membrane transporters; cytoskeletal alterations that modulate calcium homeostasis; and modulation of proteins that stabilize mitochondrial function (e.g., Bcl-2). Intervention studies in experimental stroke models have identified a battery of approaches of potential benefit in reducing neuronal death in stroke patients, including administration of antioxidants, calcium-stabilizing agents, caspase inhibitors, and agents that activate NF-kappa B. Interestingly, recent studies suggest novel dietary approaches (e.g., food restriction and supplementation with antioxidants) that may reduce brain damage following stroke.
Asunto(s)
Apoptosis/fisiología , Péptidos y Proteínas de Señalización Intracelular , Accidente Cerebrovascular/fisiopatología , Animales , Proteínas Reguladoras de la Apoptosis , Encéfalo/anatomía & histología , Encéfalo/metabolismo , Encéfalo/fisiopatología , Isquemia Encefálica/genética , Isquemia Encefálica/patología , Isquemia Encefálica/fisiopatología , Calcio/fisiología , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Núcleo Celular/genética , Núcleo Celular/fisiología , Núcleo Celular/ultraestructura , Citocinas/fisiología , Expresión Génica , Humanos , Ratones , Mitocondrias/fisiología , Modelos Biológicos , Factores de Crecimiento Nervioso/fisiología , Neuronas/citología , Neuronas/metabolismo , Neuronas/fisiología , Ratas , Especies Reactivas de Oxígeno/fisiología , Transducción de Señal , Accidente Cerebrovascular/genética , Accidente Cerebrovascular/patologíaRESUMEN
Exercise appears to increase reactive oxygen species, which can result in damage to cells. Exercise results in increased amounts of malondialdehyde in blood and pentane in breath; both serve as indirect indicators of lipid peroxidation. However, not all studies report increases; these equivocal results may be due to the large intersubject variability in response or the nonspecificity of the assays. Some studies have reported that supplementation with vitamins C and E, other antioxidants, or antioxidant mixtures can reduce symptoms or indicators of oxidative stress as a result of exercise. However, these supplements appear to have no beneficial effect on performance. Exercise training seems to reduce the oxidative stress of exercise, such that trained athletes show less evidence of lipid peroxidation for a given bout of exercise and an enhanced defense system in relation to untrained subjects. Whether the body's natural antioxidant defense system is sufficient to counteract the increase in reactive oxygen species with exercise or whether additional exogenous supplements are needed is not known, although trained athletes who received antioxidant supplements show evidence of reduced oxidative stress. Until research fully substantiates that the long-term use of antioxidants is safe and effective, the prudent recommendation for physically active individuals is to ingest a diet rich in antioxidants.
Asunto(s)
Antioxidantes/normas , Suplementos Dietéticos/normas , Ejercicio Físico/fisiología , Resistencia Física/fisiología , Especies Reactivas de Oxígeno/fisiología , Antioxidantes/química , Antioxidantes/metabolismo , Ácido Ascórbico/metabolismo , Ácido Ascórbico/normas , Estrógenos/metabolismo , Estrógenos/fisiología , Femenino , Glutatión/sangre , Humanos , Masculino , Malondialdehído/sangre , Estrés Oxidativo/fisiología , Pentanos/análisis , Especies Reactivas de Oxígeno/metabolismo , Selenio/metabolismo , Selenio/normas , Vitamina E/metabolismo , Vitamina E/normasRESUMEN
Exercise increases the generation of oxygen free radicals and lipid peroxidation. Strenuous exercise in a person who is unconditioned or unaccustomed to exercise will induce oxidative damage and result in muscle injury. However, aerobic exercise training strengthens the antioxidant defense system by increasing superoxide dismutase. Vitamin C and, especially, vitamin E are shown to decrease the exercise-induced increase in the rate of lipid peroxidation. No ergogenic effects of either vitamin C or E have been shown. Vitamin E was shown to significantly increase circulating neutrophils in older, but not younger, subjects performing eccentric exercise that causes an increase in skeletal muscle damage. In addition to its effect in augmenting the neutrophil response to eccentric exercise, vitamin E causes a greater increase in circulating creatine kinase activity, perhaps indicating increased skeletal muscle repair. Increased vitamin E intake has been associated with enhanced glucose tolerance and insulin action as well as improved lipoprotein status. Future research should examine the combined effects of exercise training and vitamins E and C on these health-related outcomes.
Asunto(s)
Antioxidantes/metabolismo , Ácido Ascórbico/fisiología , Suplementos Dietéticos/normas , Ejercicio Físico/fisiología , Vitamina E/fisiología , Adulto , Factores de Edad , Anciano , Antioxidantes/normas , Ácido Ascórbico/metabolismo , Ácido Ascórbico/normas , Creatina Quinasa/sangre , Citocinas/biosíntesis , Femenino , Humanos , Masculino , Persona de Mediana Edad , Músculo Esquelético/fisiología , Neutrófilos/química , Consumo de Oxígeno , Especies Reactivas de Oxígeno/metabolismo , Especies Reactivas de Oxígeno/fisiología , Vitamina E/metabolismo , Vitamina E/normasRESUMEN
The effects of cellular antioxidant capacity on hyperthermia (HT)-induced apoptosis and production of antiapoptotic heat shock proteins (HSPs) were investigated in HL-60 cells and in HL-60AR cells that are characterized by an elevated endogenous catalase activity. Exposure of both cell lines to 43 degrees C for 1 h initiated apoptosis. Apoptosis peaked at 3-6 h after heat exposure in the HL-60 cells. Whereas HL-60AR cells were partially protected against HT-induced apoptosis at these early time points, maximal levels of apoptosis were detected later, i.e. 12-18 h after heat exposure. This differential induction of apoptosis was directly correlated to the induction of the antiapoptotic HSP27 and HSP70. In particular, in the HL-60 cells HSP27 was significantly induced at 12-18 h after exposure to 43 degrees C when apoptosis dropped. In contrast, coinciding with the late onset of apoptosis in HL-60AR cells at that time HL-60AR cells lacked a similar HSP response. In line with the higher antioxidant capacity HL-60AR cells accumulated reactive oxygen species to a lesser degree than HL-60 cells after heat treatment. Protection from HT-induced apoptosis as well as diminished heat-induced HSP27 expression was also observed after cotreatment of HL-60 cells with 43 degrees C and catalase but not with superoxide dismutase. These data emphasize the pivotal role of reactive oxygen species for HT induced pro- and antiapoptotic pathways.
Asunto(s)
Apoptosis/fisiología , Regulación de la Expresión Génica/fisiología , Proteínas HSP70 de Choque Térmico/genética , Proteínas de Choque Térmico , Proteínas de Neoplasias/genética , Especies Reactivas de Oxígeno/fisiología , Catalasa/genética , Catalasa/metabolismo , Cumarinas/metabolismo , Colorantes Fluorescentes , Genes bcl-2 , Células HL-60 , Proteínas de Choque Térmico HSP27 , Calor , Humanos , Hipertermia Inducida , Cinética , Chaperonas Moleculares , Oligopéptidos/metabolismo , Biosíntesis de Proteínas , Proteínas Proto-Oncogénicas c-bcl-2/genética , ARN Mensajero/genética , Transcripción Genética , Proteína bcl-XRESUMEN
Over 12 consecutive years, hyperthermia was induced by immersion in hot baths 1576 times in one subject with a long history of labile hypertension. In year 13 (1996), following hyperthermia induction, peripheral lymphocytes were analyzed by Western blot analysis for heat shock protein 72 (HSP72) gene expression. It was demonstrated that rectal and oral fevers maintained for 15 mins at 38.2 C and 38. 6 C or higher, respectively, triggered HSP72 expression. Because this subject showed no evidence of organ damage secondary to hypertension, and because both hyperthermia and acute hypertension upregulate HSP72 expression, a role for HSP72 in protection against the adverse effects of essential hypertension disease is proposed and discussed.
Asunto(s)
Proteínas de Choque Térmico/metabolismo , Hipertensión/metabolismo , Western Blotting , Expresión Génica , Proteínas del Choque Térmico HSP72 , Humanos , Hipertensión/prevención & control , Hipertermia Inducida , Especies Reactivas de Oxígeno/fisiologíaRESUMEN
Although fluoroquinolone antibacterials have a broad therapeutic use, with a relatively low incidence of severe side effects, they have been reported to induce lesions in the cartilage of growing animals by a mechanism that remains unclear. This study was undertaken to determine the potentially deleterious effect of a high dose of pefloxacin (400 mg/kg of body weight) on two main constituents of cartilage in mice, i.e., proteoglycans and collagen. Variations in levels of proteoglycan anabolism measured by in vivo [(35)S]sulfate incorporation into cartilage and oxidative modifications of collagen assessed by detection of carbonyl derivatives were monitored after administration of pefloxacin. Treatment of mice with 1 day of pefloxacin treatment significantly decreased the rate of biosynthesis of proteoglycan for the first 24 h. However, no difference was observed after 48 h. The decrease in proteoglycan synthesis was accompanied by a marked drop in serum sulfate concentration and a concomitant increase in urinary sulfate excretion. The decrease in proteoglycan synthesis, also observed ex vivo, may suggest a direct effect of pefloxacin on this process, rather than it being a consequence of a low concentration of sulfate. On the other hand, treatment with pefloxacin for 10 days induced oxidative damage to collagen. In conclusion, this study demonstrates, for the first time, that pefloxacin administration to mice leads to modifications in the metabolism and integrity of extracellular proteins, such as collagen and proteoglycans, which may account for the side effects observed. These results offer new insights to explain quinolone-induced disorders in growing articular cartilage.
Asunto(s)
Antiinfecciosos/toxicidad , Artritis/inducido químicamente , Artritis/metabolismo , Cartílago Articular/metabolismo , Colágeno/metabolismo , Proteoglicanos/metabolismo , Animales , Artritis/patología , Cartílago Articular/efectos de los fármacos , Cartílago Articular/patología , Colágeno/biosíntesis , Electroforesis en Gel de Poliacrilamida , Masculino , Ratones , Oxidación-Reducción , Pefloxacina/administración & dosificación , Pefloxacina/farmacología , Proteoglicanos/biosíntesis , Especies Reactivas de Oxígeno/fisiología , Sulfatos/metabolismo , Radioisótopos de Azufre , Factores de TiempoRESUMEN
Extract from Scutellaria baicalensis Georgi Attenuates Oxidant Stress in Cardiomyocytes. Journal of Molecular and Cellular Cardiology (1999) 31, 1885-1895. Scutellaria baicalensis Georgi is a Chinese herbal medicine used to treat allergic and inflammatory diseases. The medicinal effects of S. baicalensis root may result, in part, from its constituent flavones reported to have antioxidant properties. Since oxidants play multiple roles in cells, we tested whether S. baicalensis could confer protection in a cardiomyocyte model of ischemia and reperfusion. The intracellular fluorescent probes 2',7'-dichlorofluorescin diacetate (DCFH-DA, sensitive to H(2)O(2) and hydroxyl radicals) and dihydroethidium (DHE, sensitive to superoxide) were used to assess intracellular reactive oxygen species (ROS), and propidium iodide (PI) was used to assess viability in cultured embryonic cardiomyocytes. S. baicalensis extract (SbE) quickly attenuated levels of oxidants generated during transient hypoxia and during exposure to the mitochondrial site III inhibitor antimycin A, as measured by DCFH oxidation or by DHE oxidation. These attenuated oxidant levels were associated with improved survival and function. Cell death after ischemia/reperfusion decreased from 47+/-3 % in untreated to 26+/-2 % in S. baicalensis treated cells (P<0.001). After antimycin A exposure, S. baicalensis decreased cell death from 49+/-6 % in untreated to 23+/-4 % in treated cells. Return of contraction occurred in S. baicalensis-treated cells but was not observed in control cells. Other in vitro studies revealed that baicalein, a major flavone component of SbE can directly scavenge superoxide, hydrogen peroxide, and hydroxyl radicals. Collectively, these findings indicate that SbE and its constituent flavones such as baicalein can attenuate oxidant stress and protect cells from lethal oxidant damage in an ischemia-reperfusion model.
Asunto(s)
Hipoxia de la Célula , Flavanonas , Flavonoides/farmacología , Corazón/efectos de los fármacos , Miocardio/citología , Extractos Vegetales/farmacología , Especies Reactivas de Oxígeno/fisiología , Animales , Hipoxia de la Célula/efectos de los fármacos , Supervivencia Celular , Células Cultivadas , Embrión de Pollo , Medicamentos Herbarios Chinos , Ventrículos Cardíacos , Microscopía por Video , Raíces de Plantas , Plantas MedicinalesRESUMEN
BACKGROUND AND OBJECTIVE: Whole-body infrared-A irradiation (WBIAI) according to Ardenne is a newly developed version of hyperthermia. In clinical use benefits for patients with chronic infections have been reported. In order to find out more about the immunological background of the method we studied the question whether hyperthermia leads to priming effects in human polymorphonuclear leukocytes (PMN). MATERIAL AND METHODS: We therefore investigated the production of reactive oxygen radical species (ROS) measured by lucigenin-dependent chemiluminescence after stimulation with N-formylpeptide (FMLP, 10(-6) and 10(-7) M), C5a complement (10(-7) and 10(-8) M) or phorbolester (PMA, 10(-7) and 10(-8) M) in isolated PMN of 8 volunteers undergoding a 60-min hyperthermia treatment with the WBIAI. Blood was drawn 0/60/240/510 min after the start of hyperthermia treatment. In addition, we measured blood pressure, pulse, and temperature. RESULTS: In 5 cases a significant increase in ROS (p < 0.05) could be measured beginning 240 min after start of hyperthermia and further increasing until the 510-min time point. These results suggest a priming effect in PMN lasting far beyond the actual treatment period. The increase of ROS production following stimulation with FMLP, C5a or PMA was 60.4 +/- 21.6, 86.0 +/- 23.3, and 63.3 +/- 15.9% (SEM), respectively. Moreover, in all probands the maximal ROS production in PMN was observed 510 min after the beginning of WBIAI treatment. In 3 cases no difference in ROS could be observed. There was no difference in temperature, blood pressure, and pulse between responders and nonresponders. CONCLUSIONS: Our results show a hyperthermia-dependent priming effect of ROS production in PMN, suggesting an increase in immune reaction within the observation period of 510 min. Further investigations are necessary in order to specify responders and nonresponders and to characterize the results in specific diseases and the constitutions of the patients.
Asunto(s)
Hipertermia Inducida , Rayos Infrarrojos , Neutrófilos/efectos de la radiación , Especies Reactivas de Oxígeno/fisiología , Complemento C5a/fisiología , Humanos , Técnicas In Vitro , N-Formilmetionina Leucil-Fenilalanina/farmacología , Neutrófilos/efectos de los fármacos , Neutrófilos/fisiología , Acetato de Tetradecanoilforbol/farmacología , Irradiación Corporal TotalRESUMEN
Receptors for the Fc region of IgG (Fc gamma R) mediate internalization of opsonized particles by human neutrophils (PMN) and mononuclear phagocytes. Cross-linking of Fc gamma R leads to activation of protein tyrosine kinases and phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) within Fc gamma R subunits, both obligatory early signals for phagocytosis. Human PMN constitutively express two structurally distinct Fc gamma R, Fc gamma RIIa and Fc gamma RIIIb, and can be induced to express Fc gamma RI by IFN-gamma. We have previously shown that stimulation of PMN through Fc gamma RIIIb results in enhanced Fc gamma RIIa-mediated phagocytic activity that is inhibited by catalase. In the present study, we have tested the hypothesis that reactive oxygen intermediates (ROI) have the capacity to regulate Fc gamma R responses and defined a mechanism for this effect. We show that H2O2 augmented phagocytosis mediated by Fc gamma RIIa and Fc gamma RI in PMN and amplified receptor-triggered tyrosine phosphorylation of Fc gamma R-associated ITAMs and signaling elements. Generation of endogenous oxidants in PMN by cross-linking Fc gamma RIIIb similarly enhanced phosphorylation of Fc gamma RIIa and Syk, a tyrosine kinase required for phagocytic function, in a catalase-sensitive manner. Our results provide a mechanism for priming phagocytes for enhanced responses to receptor-driven effects. ROI generated in an inflammatory milieu may stimulate quiescent cells to rapidly increase the magnitude of their effector function. Indeed, human monocytes incubated in the presence of stimulated PMN showed oxidant-induced increases in Fc gamma RIIa-mediated phagocytosis. Definition of the role of oxidants as amplifiers of Fc gamma R signaling identifies a target for therapeutic intervention in immune complex-mediated tissue injury.
Asunto(s)
Fagocitosis/inmunología , Especies Reactivas de Oxígeno/fisiología , Receptores de IgG/fisiología , Transducción de Señal/inmunología , Adyuvantes Inmunológicos/metabolismo , Adyuvantes Inmunológicos/fisiología , Línea Celular , Precursores Enzimáticos/metabolismo , Humanos , Péptidos y Proteínas de Señalización Intracelular , Monocitos/inmunología , Neutrófilos/inmunología , Neutrófilos/metabolismo , Oxidantes/metabolismo , Oxidantes/farmacología , Oxidantes/fisiología , Fosforilación/efectos de los fármacos , Proteínas Tirosina Quinasas/metabolismo , Especies Reactivas de Oxígeno/inmunología , Especies Reactivas de Oxígeno/metabolismo , Receptores de IgG/metabolismo , Quinasa Syk , Tirosina/metabolismo , Células U937RESUMEN
Hearing loss induced by ototoxicity is a worldwide problem despite the development of newer antibiotics and chemotherapy agents. The cellular mechanisms responsible for aminoglycoside-induced hearing loss are still poorly understood. We have developed two different methods of studying the dynamic cellular and subcellular changes in the chick auditory sensory epithelium that occur during hair cell death. The first study was performed in mature chicks after a single, high dose injection of gentamicin, which results in the rapid loss of all hair cells in the basal third of the cochlea. Chicks were sacrificed at discrete time points after drug treatment, and transmission electron microscopy was performed to study the ultrastructural changes in basal hair cells during the course of cell death. We noted various changes in the cell morphology including accumulation of cytoplasmic inclusion bodies, dispersion of the cytoplasmic polyribosomes, mitochondrial swelling, and cellular extrusion by 24 h after injection. The next two studies were performed using tissue cultures from mature avian auditory sensory epithelium. Cultured cells were labeled using vital fluorescent markers, and levels of intracellular calcium and reactive oxygen species within hair cells were studied following aminoglycoside exposure. We identified a dose-dependent increase in the levels of intracellular calcium, which was blocked by an inhibitor of voltage-gated calcium channels. We also found that levels of reactive oxygen species in hair cells greatly increased after exposure to gentamicin, and this response was blocked by two different antioxidants. These studies serve to identify key cellular and molecular changes in hair cells in response to ototoxic drugs. Further study of these processes may lead to a better understanding of how ototoxicity is induced and to potential preventative interventions.
Asunto(s)
Antibacterianos/efectos adversos , Calcio/metabolismo , Gentamicinas/efectos adversos , Células Ciliadas Auditivas/efectos de los fármacos , Animales , Muerte Celular/efectos de los fármacos , Células Cultivadas , Pollos , Femenino , Radicales Libres/metabolismo , Células Ciliadas Auditivas/ultraestructura , Especies Reactivas de Oxígeno/fisiologíaRESUMEN
The evidence that the generation of reactive oxygen intermediates (ROI) plays an important role for the increased cardiovascular risk in diabetes is summarised. In addition to the well known parameters of oxidative stress as lipid hydroperoxides and thiobarbituric acid substances (TBARS), recent observations indicate that isoprostanes which can be taken as a more specific parameter of oxidative stress, are generated in higher amounts by diabetic patients. This increased formation of isoprostanes can be inhibited by an installment of a close metabolic control or the supplementation with tocopherol. The cause for the elevated oxidative stress is not yet fully understood, however the autoxidation of glucose, the formation of advanced glycation endproducts and the activation of NADPH-oxidase seem to be relevant processes. Since ROI are able to quench nitric oxide and to inhibit the synthesis of prostacyclin, the antithrombotic, vasodilating and antiatherosclerotic properties of endothelium are impaired in diabetes. Additionally, the balance of endothelial mediators released by endothelium is shifted to angiotensin II and endothelin, compounds which enhance the proliferation of smooth muscle cells and may limit the coronary reserve of myocardium. The activation of the transcription factor NF-kappa B by glucose and its autoxidative products is regarded as a key event in the transformation of the vasculature in diabetes. Epidemiological observations and very recent clinical studies underlie the impact of ROI for the development of cardiovascular complications in diabetes and suggest that an antioxidative treatment might be helpful to reduce the cardiac risk in diabetes.
Asunto(s)
Cardiomiopatías/metabolismo , Cardiomiopatías/prevención & control , Angiopatías Diabéticas/metabolismo , Angiopatías Diabéticas/prevención & control , Especies Reactivas de Oxígeno/fisiología , Vitamina E/uso terapéutico , Animales , Cardiomiopatías/fisiopatología , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/fisiopatología , Angiopatías Diabéticas/fisiopatología , Radicales Libres/metabolismo , HumanosRESUMEN
It seems clear that the abundance of potential treatment options reflects the dearth of proved, effective options. Thus, although we appear to be on the brink of many potentially major breakthroughs in treatment, there currently remains a multitude of unanswered questions and the need for further study. At this point clinical recommendations must be limited to supportive care with moderation: oxygenation without hyperoxia; ventilation without hypocarbia; avoiding extremes of blood pressure, hematocrit, blood glucose, and body temperature. Unfortunately, data from human trials are extremely limited and often poorly controlled. Furthermore, even those few existing human studies have rarely--if ever--dealt with newborns infants (Table 2). In addition, many of the existing studies do not relate to generalized asphyxia but rather to single-organ reperfusion insults. Finally, there is the critical issue of timing. Unfortunately, much of the existing experimental data relate to prophylaxis rather than treatment, severely limiting their potential for clinical applicability. Interventions may have quite different effects when administered at different phases of this most intricate process. Hyperglycemia, for example, may be neuroprotective before an insult but detrimental if induced after an asphyxial episode. Conversely, the NMDA blocker MK-801 can adversely affect outcome when given before a global asphyxial insult but can reduce seizure-related damage when given during the hyperexcitability phase. Insulin-like growth factor is also neuroprotective only when given after an insult, but it is not helpful if given before. An intimate understanding of the pathophysiologic processes involved is essential before any attempts at applying the diverse data derived from numerous animal studies to the human situation in an intelligent manner. Future studies may focus on cocktails of different mixtures of the compounds discussed or on single multipotential drugs, which would make possible a multipronged approach. However, it is essential to investigate fully the potential for toxic drug interactions, as some combinations may be produce serious consequences. For example, Gluckman and Williams evaluated the potential of combining calcium channel blockers with NMDA receptor antagonists in hypoxic-ischemic rats and found that this combination led to rapid cardiovascular collapse. Other enticing approaches for future investigations will probably include some genetic-engineering-related studies in attempt to enhance endogenous antioxidant defenses with regulon stimulation or the administration of neurotrophic growth factors. Unavoidably, the trip from the laboratory to the bedside must of necessity be an arduous and rigorous one.
Asunto(s)
Asfixia Neonatal/terapia , Daño por Reperfusión/etiología , Daño por Reperfusión/fisiopatología , Animales , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Humanos , Recién Nacido , Inflamación , Ratas , Especies Reactivas de Oxígeno/fisiología , Daño por Reperfusión/tratamiento farmacológico , Factores de RiesgoRESUMEN
Recent research has indicated that increased vulnerability to oxidative stress may be the major factor involved in CNS functional declines in aging and age-related neurodegenerative diseases, and that antioxidants, e.g., vitamin E, may ameliorate or prevent these declines. Present studies examined whether long-term feeding of Fischer 344 rats, beginning when the rats were 6 months of age and continuing for 8 months, with diets supplemented with a fruit or vegetable extract identified as being high in antioxidant activity, could prevent the age-related induction of receptor-mediated signal transduction deficits that might have a behavioral component. Thus, the following parameters were examined: (1) oxotremorine-enhanced striatal dopamine release (OX-K+-ERDA), (2) cerebellar beta receptor augmentation of GABA responding, (3) striatal synaptosomal 45Ca2+ clearance, (4) carbachol-stimulated GTPase activity, and (5) Morris water maze performance. The rats were given control diets or those supplemented with strawberry extracts (SE), 9.5 gm/kg dried aqueous extract (DAE), spinach (SPN 6.4 gm/kg DAE), or vitamin E (500 IU/kg). Results indicated that SPN-fed rats demonstrated the greatest retardation of age-effects on all parameters except GTPase activity, on which SE had the greatest effect, whereas SE and vitamin E showed significant but equal protection against these age-induced deficits on the other parameters. For example, OX-K+-ERDA enhancement was four times greater in the SPN group than in controls. Thus, phytochemicals present in antioxidant-rich foods such as spinach may be beneficial in retarding functional age-related CNS and cognitive behavioral deficits and, perhaps, may have some benefit in neurodegenerative disease.