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1.
World J Microbiol Biotechnol ; 37(4): 66, 2021 Mar 19.
Artículo en Inglés | MEDLINE | ID: mdl-33740144

RESUMEN

Pseudomonas aeruginosa is one of the vulnerable opportunistic pathogens associated with nosocomial infections, cystic fibrosis, burn wounds and surgical site infections. Several studies have reported that quorum sensing (QS) systems are controlled the P. aeruginosa pathogenicity. Hence, the targeting of QS considered as an alternative approach to control P. aeruginosa infections. This study aimed to evaluate the anti-quorum sensing and antibiofilm inhibitory potential of Musa paradisiaca against Chromobacterium violaceum (ATCC 12472) and Pseudomonas aeruginosa. The methanol extract of M. paradisiacsa exhibits that better antibiofilm potential against P. aeruginosa. Then, the crude methanol extract was subjected to purify by column chromatography and collected the fractions. The mass-spectrometric analysis of a methanol extract of M. paradisiaca revealed that 1,8-cineole is the major compounds. 1, 8-cineole significantly inhibited the QS regulated violacein production in C. violaceum. Moreover, 1,8-cineole significantly inhibited the QS mediated virulence production and biofilm formation of P. aeruginosa without affecting their growth. The real-time PCR analysis showed the downregulation of autoinducer synthase and transcriptional regulator genes upon 1,8-cineole treatment. The findings of the present study strongly suggested that metabolite of M. paradisiaca impedes P. aeruginosa QS system and associated virulence productions.


Asunto(s)
Antibacterianos/farmacología , Biopelículas/efectos de los fármacos , Eucaliptol/química , Eucaliptol/farmacología , Musa/química , Pseudomonas aeruginosa/efectos de los fármacos , Percepción de Quorum/efectos de los fármacos , Alginatos/metabolismo , Biopelículas/crecimiento & desarrollo , Chromobacterium/efectos de los fármacos , Eucaliptol/aislamiento & purificación , Expresión Génica/efectos de los fármacos , Glucolípidos/biosíntesis , India , Pruebas de Sensibilidad Microbiana , Extractos Vegetales/farmacología , Polisacáridos Bacterianos , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/crecimiento & desarrollo , Piocianina/biosíntesis , Virulencia/efectos de los fármacos , Factores de Virulencia
2.
Eur J Pharmacol ; 890: 173669, 2021 Jan 05.
Artículo en Inglés | MEDLINE | ID: mdl-33098832

RESUMEN

Glioma is the most common primary intracranial tumor, in which glioblastoma (GBM) is the most malignant and lethal. However, the current chemotherapy drugs are still unsatisfactory for GBM therapy. As the natural products mainly extracted from Eucalyptus species, phloroglucinol-terpene adducts have the potential to be anti-cancer lead compounds that attracted increasing attention. In order to discover the new lead compounds with the anti-GBM ability, we isolated Eucalyptal A with a phloroglucinol-terpene skeleton from the fruit of E. globulus and investigated its anti-GBM activity in vitro and in vivo. Functionally, we verified that Eucalyptal A could inhibit the proliferation, growth and invasiveness of GBM cells in vitro. Moreover, Eucalyptal A had the same anti-GBM activity in tumor-bearing mice as in vitro and prolonged the overall survival time by maintaining mice body weight. Further mechanism research revealed that Eucalyptal A downregulated SRSF1 expression and rectified SRSF1-guided abnormal alternative splicing of MYO1B mRNA, which led to anti-GBM activity through the PDK1/AKT/c-Myc and PAK/Cofilin axes. Taken together, we identified Eucalyptal A as an important anti-GBM lead compound, which represents a novel direction for glioma therapy.


Asunto(s)
Neoplasias Encefálicas/metabolismo , Carcinogénesis/efectos de los fármacos , Eucaliptol/uso terapéutico , Glioma/metabolismo , Miosina Tipo I/metabolismo , Empalme de Proteína/efectos de los fármacos , Factores de Empalme Serina-Arginina/biosíntesis , Animales , Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/uso terapéutico , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/prevención & control , Carcinogénesis/metabolismo , Carcinogénesis/patología , Línea Celular Tumoral , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Medicamentos Herbarios Chinos/uso terapéutico , Eucaliptol/aislamiento & purificación , Eucaliptol/farmacología , Regulación Neoplásica de la Expresión Génica , Glioma/genética , Glioma/prevención & control , Humanos , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Miosina Tipo I/genética , Empalme de Proteína/fisiología , ARN Mensajero/genética , ARN Mensajero/metabolismo , Factores de Empalme Serina-Arginina/antagonistas & inhibidores , Factores de Empalme Serina-Arginina/genética , Ensayos Antitumor por Modelo de Xenoinjerto/métodos
3.
Am J Chin Med ; 48(3): 597-613, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32308013

RESUMEN

Magnoliae Flos is a commonly used traditional medicinal material in Asia. It is used to treat sinusitis, nasal congestion, and hypersensitive skin. Because Magonlia Flos was described as an aromatic material in ancient Chinese texts, we hypothesized that its essential oil may be used to treat immune disorders. Dendritic cells (DCs), regarded as a major target of immunomodulators to control immune responses, play a critical role in the adaptive immune response. In this study, Magnoliae Flos essential oil (MFEO) decreased the production of the cytokines TNF-α, IL-6, and IL-12p70 in lipopolysaccharide (LPS)-stimulated DCs. It also suppressed the surface markers MHC II, CD80, and CD86 in LPS-stimulated DCs. Animal models demonstrated that the 2,4-Dinitro-1-fluorobenzene (DNFB) inducing a contact hypersensitivity response was inhibited following treatment with MFEO. In addition, MFEO inhibited the infiltration of T cells in the ears of DNFB-induced mice. To explore its bioactive compounds, the components of MFEO were analyzed using gas chromatography (GC) and GC-mass spectrometry. The results revealed that the major compounds in MFEO are camphor and 1,8-cineole. Additional DC bioassays confirmed that these compounds substantially suppressed cytokine production in LPS-induced DCs. Therefore, we demonstrated that MFEO exhibits an immunosuppressive effect both in vivo and in vitro, and camphor and 1,8-cineole may be the major components responsible for its immunosuppressive ability. The findings indicate that MFEO has the potential to be developed as a new immunosuppressant for excessive diseases.


Asunto(s)
Inmunidad Adaptativa/efectos de los fármacos , Células Dendríticas/inmunología , Dermatitis por Contacto/tratamiento farmacológico , Dermatitis por Contacto/inmunología , Inmunosupresores , Magnoliaceae/química , Aceites Volátiles/farmacología , Aceites Volátiles/uso terapéutico , Fitoterapia , Animales , Alcanfor/análisis , Alcanfor/aislamiento & purificación , Células Cultivadas , Citocinas/metabolismo , Células Dendríticas/metabolismo , Modelos Animales de Enfermedad , Eucaliptol/análisis , Eucaliptol/aislamiento & purificación , Ratones , Aceites Volátiles/química , Linfocitos T/inmunología
4.
Molecules ; 23(11)2018 Oct 30.
Artículo en Inglés | MEDLINE | ID: mdl-30380739

RESUMEN

To highlight the importance of the spices in the Mediterranean diet, the aim of the paper was to study the essential oil compositions and to clarify the potential differences in the biological activities of the three cardamom species. In the study, we compared the phytochemical profiles and biological activities of essential oils from Elettaria cardamomum, Aframomum corrorima and Amomum subulatum. The oils were analyzed using the GC and GC/MS techniques and were mainly constituted of the oxygenated monoterpenes which represents 71.4%, 63.0%, and 51.0% of all compounds detected in E. cardamomum, A. corrorima and A. subulatum essential oils, respectively, 1,8-cineole was the main common compound between the tree tested volatile oil. The essential oils showed significant antimicrobial activity against Gram-positive and Gram-negative microorganisms tested especially the fungal strains. The Ethiopian cardamom was the most active essential oil with fungal growth inhibition zone ranging from 12.67 to 34.33 mm, MICs values ranging from 0.048 to 0.19 mg/mL, and MBCs values from 0.19 to 1.75 mg/mL. The three tested essential oils and their main component (1,8-cineole) significantly increased the production of elastase and protease production, and motility in P. aeruginosa PAO1 in a dose dependent manner. In fact, at 10 mg/mL concentration, the three essential oils showed more than 50% of inhibition of elastolytic and proteolytic activities in P. aeruginosa PAO1. The same oils inhibited also the violacein production in C. violaceum strain. It was also noticed that at high concentrations, the A. corrorima essential oil significantly inhibited the germination of radish. A thorough knowledge of the biological and safety profiles of essential oils can produce applications of economic importance.


Asunto(s)
Amomum/química , Antiinfecciosos/química , Elettaria/química , Aceites Volátiles/química , Antiinfecciosos/farmacología , Eucaliptol/química , Eucaliptol/aislamiento & purificación , Eucaliptol/farmacología , Hongos/efectos de los fármacos , Cromatografía de Gases y Espectrometría de Masas , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Aceites de Plantas/química , Aceites de Plantas/farmacología
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