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1.
Life Sci ; 144: 49-53, 2016 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-26631504

RESUMEN

AIMS: Acne is a common skin disease that originates in the sebaceous gland. The pathogenesis of acne is very complex, involving the increase of sebum production and perifollicular inflammation. In this study, we screened the anti-lipogenic material and demonstrated its effect using cultured human sebocytes. MAIN METHODS: Normal human sebocytes were cultured by explanting the sebaceous glands. To evaluate the anti-lipogenic effect, sebocytes were treated with test materials and (14)C-acetate incorporation assay was performed. KEY FINDINGS: To screen the anti-lipogenic materials, we tested the effect of many herbal plant extracts. We found that Angelica dahurica extract inhibited the insulin-like growth factor-1 (IGF-1)-induced sebum production in terms of squalene synthesis in sebocytes. Furthermore, imperatorin isolated from A. dahurica showed remarkable inhibitory effect on squalene production as well as squalene synthase promoter activity. To investigate the putative action mechanism, we tested the effect of imperatorin on intracellular signaling. The results showed that imperatorin inhibited IGF-1-induced phosphorylation of Akt. In addition, imperatorin significantly down-regulated PPAR-γ and SREBP-1, the important transcription factors for lipid synthesis. SIGNIFICANCE: These results suggest that imperatorin has a potential for reducing sebum production in sebocytes, and can be applicable for acne treatment.


Asunto(s)
Furocumarinas/farmacología , Factor I del Crecimiento Similar a la Insulina/antagonistas & inhibidores , Factor I del Crecimiento Similar a la Insulina/farmacología , Sebo/metabolismo , Angelica/química , Células Cultivadas , Farnesil Difosfato Farnesil Transferasa/antagonistas & inhibidores , Farnesil Difosfato Farnesil Transferasa/efectos de los fármacos , Farnesil Difosfato Farnesil Transferasa/genética , Humanos , Lipogénesis/efectos de los fármacos , Fosforilación/efectos de los fármacos , Extractos Vegetales/farmacología , Proteínas Proto-Oncogénicas c-akt/efectos de los fármacos , Proteínas Proto-Oncogénicas c-akt/metabolismo , Glándulas Sebáceas/citología , Glándulas Sebáceas/efectos de los fármacos , Glándulas Sebáceas/metabolismo , Sebo/efectos de los fármacos , Piel/citología , Piel/efectos de los fármacos , Piel/metabolismo , Escualeno/metabolismo
2.
Plant Cell Physiol ; 43(12): 1502-9, 2002 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-12514247

RESUMEN

Pectin administered to Uncaria tomentosa cell suspension cultures, was found to increase the production of triterpene acids (ursolic and oleanolic acid), however, neither growth nor sterol accumulation were affected. Cell cultures showed that pectin treatment caused a rapid threefold increase in the activities of enzymes involved in the biosynthesis of C(5) and C(30 )isoprenoid, such as isopentenyl diphosphate isomerase and squalene synthase. The activity of a farnesyl diphosphatase, which could divert the flux of farnesyl diphosphate to farnesol, was two times lower in elicited than in control cells. Elicited cells also transformed more rapidly a higher percentage of [5-(3)H]mevalonic acid into triterpene acids. Interestingly, addition of terbinafine, an inhibitor of squalene epoxidase, to elicited cell cultures inhibited sterol accumulation while triterpene production was not inhibited. These results suggest that in U. tomentosa cells, both the previously mentioned enzymes and those involved in squalene 2,3-oxide formation play an important regulatory role in the biosynthesis of sterols and triterpenes.


Asunto(s)
Uña de Gato/metabolismo , Fitosteroles/biosíntesis , Triterpenos/metabolismo , Isomerasas de Doble Vínculo Carbono-Carbono/efectos de los fármacos , Isomerasas de Doble Vínculo Carbono-Carbono/metabolismo , Uña de Gato/citología , Uña de Gato/efectos de los fármacos , Células Cultivadas , Inhibidores Enzimáticos/farmacología , Farnesil Difosfato Farnesil Transferasa/efectos de los fármacos , Farnesil Difosfato Farnesil Transferasa/metabolismo , Hemiterpenos , Ácido Mevalónico/metabolismo , Naftalenos/farmacología , Ácido Oleanólico/metabolismo , Oxigenasas/efectos de los fármacos , Pectinas/farmacología , Monoéster Fosfórico Hidrolasas/efectos de los fármacos , Monoéster Fosfórico Hidrolasas/metabolismo , Sitoesteroles/antagonistas & inhibidores , Escualeno/antagonistas & inhibidores , Escualeno-Monooxigenasa , Terbinafina , Tritio/metabolismo , Ácido Ursólico
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