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Métodos Terapéuticos y Terapias MTCI
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1.
Dalton Trans ; 45(21): 8845-53, 2016 Jun 07.
Artículo en Inglés | MEDLINE | ID: mdl-27147084

RESUMEN

Presently most bacteria are becoming antibiotic resistant. Due to this there is a deficiency of potent antibiotics, therefore we have to preserve and improve the efficiency of existing antibiotics by mitigating the side effects. Enrofloxacin (EFX) is an important antimicrobial used in veterinary practice but it is known to exert immune suppression antioxidant stress. In the present study, we report on: (a) the biosynthesis of selenium nanoparticles (Se NPs), and (b) their protective effect in reducing adverse effects of EFX on broiler chicken. A potent bacterial strain, isolated from farm soil, has been identified as Pantoea agglomerans (GenBank: KU500622). It tolerates a high concentration of selenium dioxide (9 mM) and produces Se NPs under aerobic conditions. The obtained Se NPs are amorphous in structure and spherical in shape with sizes of less than 100 nm. The activity of cellular, humoral immune response and enzymatic and non-enzymatic antioxidants, has significantly been decreased as a result of EFX treatment. We investigated that Se NP supplementation greatly restores these values towards the control, and to even higher than those of the control. Adverse effects of EFX are prevented by simultaneous exposure to Se NPs (0.6 mg per kg of feed) in the diet of poultry chicken.


Asunto(s)
Fluoroquinolonas/toxicidad , Inmunidad Celular/efectos de los fármacos , Nanopartículas/química , Estrés Oxidativo/efectos de los fármacos , Sustancias Protectoras/farmacología , Óxidos de Selenio/química , Animales , Antioxidantes/metabolismo , Pollos , Suplementos Dietéticos , Enrofloxacina , Fluoroquinolonas/aislamiento & purificación , Inmunidad Humoral/efectos de los fármacos , Nanopartículas/administración & dosificación , Nanopartículas/metabolismo , Pantoea/metabolismo , Tamaño de la Partícula , Fitohemaglutininas/metabolismo , Sustancias Protectoras/química
2.
Electrophoresis ; 33(19-20): 2978-86, 2012 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23018278

RESUMEN

An easy, selective, and sensitive method has been developed for the determination of enrofloxacin (ENR) and its main active metabolite, ciprofloxacin (CIP), in raw bovine milk using CE with UV detection at 268 nm. Milk samples were prepared by a clean-up/extraction procedure based on protein precipitation with hydrochloride acid followed by being defatted by centrifugation and SPE using a hydrophilic-lipophilic balance cartridge. Optimum separation was obtained using a 50 mM phosphoric acid at pH 8.4 and the total electrophoretic run time was 6 min. Sample preparation by this method yielded clean extracts with quantitative and consistent mean recoveries from 89 to 97% for CIP and from 93 to 98% for ENR. LODs obtained were lower to the maximum residue limits for these fluoroquinolones. The precision of the ensuing method is acceptable; thus, the RSD for peak area and migration time was less than 8.5 and 0.5% for CIP and 9.9 and 0.9% for ENR, respectively. The results showed that the proposed method was efficient showing good recoveries, sensitivity, and precision for the studied compounds and could be satisfactorily applied in routine analysis for the monitoring of ENR and CIP residues in milk, due to its ruggedness and feasibility demonstrated.


Asunto(s)
Ciprofloxacina/análisis , Residuos de Medicamentos/análisis , Fluoroquinolonas/análisis , Leche/química , Animales , Bovinos , Fraccionamiento Químico , Ciprofloxacina/aislamiento & purificación , Residuos de Medicamentos/aislamiento & purificación , Electroforesis Capilar/métodos , Enrofloxacina , Fluoroquinolonas/aislamiento & purificación , Límite de Detección , Reproducibilidad de los Resultados , Temperatura
3.
Ann Biol Clin (Paris) ; 63(1): 89-92, 2005.
Artículo en Francés | MEDLINE | ID: mdl-15689317

RESUMEN

Infrared spectrophotometric analysis (FTIR) was performed on a crystalline deposit developed in a corneal ulcer by an old woman who received ciprofloxacine ophthalmic drops. We collected the data of the literature on the subject. After in vitro crystallization experiments, we conclude that ciprofloxacin and norfloxacin corneal precipitates occur at physiological lachrymal pH with magnesium.


Asunto(s)
Ciprofloxacina/aislamiento & purificación , Ciprofloxacina/uso terapéutico , Úlcera de la Córnea/patología , Úlcera de la Córnea/fisiopatología , Norfloxacino/uso terapéutico , Anciano , Ciprofloxacina/administración & dosificación , Cristalización , Femenino , Fluoroquinolonas/aislamiento & purificación , Fluoroquinolonas/uso terapéutico , Humanos , Concentración de Iones de Hidrógeno , Magnesio/farmacología , Norfloxacino/administración & dosificación , Norfloxacino/aislamiento & purificación , Soluciones Oftálmicas , Espectroscopía Infrarroja por Transformada de Fourier , Lágrimas/efectos de los fármacos , Lágrimas/fisiología
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