RESUMEN
Ultraviolet radiation (UVr) promotes several well-known molecular changes, which may ultimately impact on health. Some of these effects are detrimental, like inflammation, carcinogenesis and immunosuppression. On the other hand, UVr also promotes vitamin D synthesis and other beneficial effects. We recently demonstrated that exposure to very low doses of UVr on four consecutive days [repetitive low UVd (rlUVd)] does not promote an inflammatory state, nor the recruitment of neutrophils or lymphocytes, as the exposure to a single high UV dose (shUVd) does. Moreover, rlUVd reinforce the epithelium by increasing antimicrobial peptides transcription and epidermal thickness. The aim of this study was to evaluate the adaptive immune response after shUVd and rlUVd, determining T-cell and B-cell responses. Finally, we challenged animals exposed to both irradiation procedures with Staphylococcus aureus to study the overall effects of both innate and adaptive immunity during a cutaneous infection. We observed, as expected, a marked suppression of T-cell and B-cell responses after exposure to an shUVd but a novel and significant increase in both specific responses after exposure to rlUVd. However, the control of the cutaneous S. aureus infection was defective in this last group, suggesting that responses against pathogens cannot be ruled out from isolated stimuli.
Asunto(s)
Inmunidad Adaptativa/efectos de la radiación , Exposición a la Radiación , Rayos Ultravioleta , Animales , Formación de Anticuerpos/inmunología , Formación de Anticuerpos/efectos de la radiación , Linfocitos B/inmunología , Linfocitos B/metabolismo , Linfocitos B/efectos de la radiación , Biomarcadores , Citocinas/metabolismo , Dermatitis/inmunología , Dermatitis/metabolismo , Dermatitis/microbiología , Dermatitis/prevención & control , Modelos Animales de Enfermedad , Inmunización , Inmunofenotipificación , Masculino , Ratones , Dosis de Radiación , Infecciones Cutáneas Estafilocócicas/inmunología , Infecciones Cutáneas Estafilocócicas/microbiología , Infecciones Cutáneas Estafilocócicas/prevención & control , Staphylococcus aureus/inmunología , Staphylococcus aureus/efectos de la radiación , Linfocitos T/inmunología , Linfocitos T/metabolismo , Linfocitos T/efectos de la radiación , Toxoide Tetánico/administración & dosificación , Toxoide Tetánico/inmunologíaAsunto(s)
Anticuerpos Antinucleares/sangre , Enfermedades Autoinmunes/inmunología , Enfermedades Autoinmunes/radioterapia , Enfermedades de la Piel/inmunología , Enfermedades de la Piel/radioterapia , Terapia Ultravioleta/efectos adversos , Adulto , Formación de Anticuerpos/efectos de la radiación , Femenino , Humanos , Estudios Longitudinales , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
Whole body irradiated mice appear to experience a down-regulation of the helper T (Th)1-like immune response, and maintain a persistent immunological imbalance. In the current study, we evaluated the effect of HemoHIM (an herbal product made from Angelica Radix, Cnidium officinale , and Paeonia japonica cultivated in Korea) to ameliorate the immunological imbalance induce in fractionated γ-irradiated mice. The mice were exposed to γ rays twice a week (0.5 Gy fractions) for a total dose of 5 Gy, and HemoHIM was administrated orally from 1 week before the first irradiation to 1 week before the final analysis. All experiments were performed 4 and 6 months after their first exposure. HemoHIM ameliorated the Th1- and Th2-related immune responses normally occur in irradiated mice with or without dinitrophenylated keyhole limpet hemocyanin immunization. HemoHIM also restored the natural killer cell activities without changing the percentage of natural killer cells in irradiated mice. Furthermore, the administration of HemoHIM prevented the reduction in levels of interleukin-12p70 in irradiated mice. Finally, we found that HemoHIM enhanced the phosphorylation of signal transducer and activator of transcription (STAT) 4 that was reduced in irradiated mice. Our findings suggest that HemoHIM ameliorates the persistent down-regulation of Th1-like immune responses by modulating the IL-12p70/pSTAT4 signaling pathway.
Asunto(s)
Rayos gamma/efectos adversos , Síndromes de Inmunodeficiencia/prevención & control , Interleucina-12/fisiología , Células Asesinas Naturales/efectos de los fármacos , Fitoterapia , Extractos Vegetales/uso terapéutico , Traumatismos Experimentales por Radiación/tratamiento farmacológico , Protectores contra Radiación/uso terapéutico , Factor de Transcripción STAT4/fisiología , Transducción de Señal/efectos de los fármacos , Células TH1/efectos de los fármacos , Irradiación Corporal Total/efectos adversos , Animales , Formación de Anticuerpos/efectos de los fármacos , Formación de Anticuerpos/efectos de la radiación , Citotoxicidad Inmunológica/efectos de los fármacos , Citotoxicidad Inmunológica/efectos de la radiación , Fraccionamiento de la Dosis de Radiación , Regulación hacia Abajo/efectos de los fármacos , Evaluación Preclínica de Medicamentos , Femenino , Inmunización , Síndromes de Inmunodeficiencia/etiología , Células Asesinas Naturales/inmunología , Células Asesinas Naturales/efectos de la radiación , Linfocinas/metabolismo , Ratones , Ratones Endogámicos C57BL , Fosforilación/efectos de los fármacos , Extractos Vegetales/farmacología , Procesamiento Proteico-Postraduccional/efectos de los fármacos , Traumatismos Experimentales por Radiación/inmunología , Protectores contra Radiación/farmacología , Organismos Libres de Patógenos Específicos , Bazo/inmunología , Bazo/patología , Bazo/efectos de la radiación , Células TH1/metabolismo , Células TH1/efectos de la radiación , Células Th2/efectos de los fármacos , Células Th2/metabolismo , Células Th2/efectos de la radiaciónAsunto(s)
Formación de Anticuerpos/efectos de la radiación , Absceso Pulmonar/inmunología , Absceso Pulmonar/radioterapia , Terapia Ultravioleta/métodos , Enfermedad Aguda , Adulto , Anciano , Femenino , Humanos , Absceso Pulmonar/microbiología , Masculino , Persona de Mediana Edad , Resultado del TratamientoRESUMEN
A single dose of 6 Gy irradiation significantly reduced the total WBC count while in herbal formulation (AC II) treated groups it was found to be significantly increased. Similarly bone marrow cellularity and alpha-esterase positive cells, which were lowered by radiation, were partly restored in AC II treated groups. The data indicate that AC II can overcome the immunosuppression produced by irradiation.
Asunto(s)
Adyuvantes Inmunológicos/uso terapéutico , Rayos gamma , Tolerancia Inmunológica , Medicina Ayurvédica , Preparaciones de Plantas/uso terapéutico , Traumatismos Experimentales por Radiación/tratamiento farmacológico , Adyuvantes Inmunológicos/administración & dosificación , Administración Oral , Animales , Formación de Anticuerpos/efectos de los fármacos , Formación de Anticuerpos/efectos de la radiación , Peso Corporal/efectos de los fármacos , Peso Corporal/efectos de la radiación , Células de la Médula Ósea/citología , Células de la Médula Ósea/efectos de los fármacos , Células de la Médula Ósea/efectos de la radiación , Esterasas/metabolismo , Técnica de Placa Hemolítica , Tolerancia Inmunológica/efectos de los fármacos , Tolerancia Inmunológica/efectos de la radiación , Ratones , Ratones Endogámicos BALB C , Preparaciones de Plantas/administración & dosificación , Traumatismos Experimentales por Radiación/inmunología , Bazo/citología , Bazo/efectos de los fármacos , Bazo/efectos de la radiaciónRESUMEN
Laser iridotherapy was carried out simultaneously with irradiation of immuno-competent zones on the integument of the human body in patients with stomach and duodenal ulcers. As a result of such treatment, it was discovered that laser therapy has immunomodulating action leading to the reduction of the ulcer cicatrisation period.
Asunto(s)
Úlcera Duodenal/inmunología , Infecciones por Helicobacter/inmunología , Terapia por Luz de Baja Intensidad/métodos , Úlcera Gástrica/inmunología , Adulto , Formación de Anticuerpos/efectos de los fármacos , Formación de Anticuerpos/efectos de la radiación , Úlcera Duodenal/tratamiento farmacológico , Úlcera Duodenal/microbiología , Úlcera Duodenal/radioterapia , Duodeno/efectos de los fármacos , Duodeno/microbiología , Duodeno/patología , Duodeno/efectos de la radiación , Endoscopía del Sistema Digestivo , Infecciones por Helicobacter/tratamiento farmacológico , Infecciones por Helicobacter/microbiología , Infecciones por Helicobacter/radioterapia , Helicobacter pylori/efectos de los fármacos , Helicobacter pylori/aislamiento & purificación , Helicobacter pylori/efectos de la radiación , Humanos , Inmunidad Celular/efectos de los fármacos , Inmunidad Celular/efectos de la radiación , Recuento de Linfocitos , Estómago/efectos de los fármacos , Estómago/microbiología , Estómago/patología , Estómago/efectos de la radiación , Úlcera Gástrica/tratamiento farmacológico , Úlcera Gástrica/microbiología , Úlcera Gástrica/radioterapia , Células del Estroma/efectos de los fármacos , Células del Estroma/efectos de la radiación , Linfocitos T/citología , Linfocitos T/efectos de los fármacos , Linfocitos T/efectos de la radiación , Resultado del TratamientoRESUMEN
The aim of our investigation was to study the influence of low-intensity laser therapy at the immunologic parameters and Helicobacter pylori (HP) microbial contamination in patients with exacerbation of peptic and duodenal ulcer. 100 patients aged 18-65 were examined and divided into 2 groups. The experimental group (75 patients) underwent a complex drug and laser therapy of various therapeutic techniques. The control group (25 patients) underwent only drug therapy. The results of this study show the immunocorrective effect of different methods of low-intensity laser therapy in the exacerbation period and more essential decrease of HP microbial contamination in the experimental group.
Asunto(s)
Formación de Anticuerpos/efectos de la radiación , Terapia por Luz de Baja Intensidad/métodos , Neutrófilos/efectos de la radiación , Úlcera Péptica/inmunología , Fagocitosis/efectos de la radiación , Adulto , Antibacterianos/uso terapéutico , Antiulcerosos/uso terapéutico , Infecciones por Helicobacter/tratamiento farmacológico , Infecciones por Helicobacter/inmunología , Infecciones por Helicobacter/microbiología , Infecciones por Helicobacter/radioterapia , Helicobacter pylori/efectos de los fármacos , Helicobacter pylori/aislamiento & purificación , Helicobacter pylori/efectos de la radiación , Humanos , Inmunoglobulinas/análisis , Úlcera Péptica/tratamiento farmacológico , Úlcera Péptica/microbiología , Úlcera Péptica/radioterapia , Resultado del TratamientoRESUMEN
Ultraviolet (UV) light is known to induce skin cancers by causing DNA gene mutations and inducing immunosuppression. Taking advantage of these immunosuppressive capacities, UV light has been used, with different modalities, as an immunosuppressive therapy in a variety of diseases including allograft rejection and graft-versus-host disease. Phototherapy includes UVB irradiation, UVA irradiation, oral psoralen (+)UVA irradiation (PUVA), photodynamic therapy, and extracorporeal photopheresis, which consists of infusion of UVA-irradiated autologous leukocytes collected by apheresis and incubated with 8-methoxypsoralen. According to numerous experimental models and human data, there is increasing evidence that UVB irradiation and extracorporeal photopheresis can induce regulatory T cells and anticlonotypic activity. These therapies induce apoptosis of activated T cells or of extracorporally treated mononuclear cells, and up-regulate the expression of costimulary molecules and adhesion molecules on antigen presenting cells. UVB- or UVA-induced apoptotic cells could secrete immune suppressive cytokines (interleukin (IL)-4, IL-10). The processing and presentation of apoptotic T cell antigens from clones of pathogenic T cells by activated antigen presenting cells might explain the induction of systemic anticlonotypic activity by photopheresis. This induction of cell-mediated suppressive activity opens up future prospects with the aim of expanding regulatory T cells and/or anticlonotypic activity, especially by photopheresis in organ and cell transplantation.
Asunto(s)
Linfocitos T Reguladores/fisiología , Linfocitos T Reguladores/efectos de la radiación , Rayos Ultravioleta , Animales , Formación de Anticuerpos/efectos de la radiación , Apoptosis/efectos de la radiación , División Celular/efectos de la radiación , Dermatitis por Contacto/prevención & control , Humanos , Hipersensibilidad Tardía/prevención & control , Neoplasias Cutáneas/patología , Tolerancia al TrasplanteAsunto(s)
Balneología , Rayos Infrarrojos/uso terapéutico , Terapia por Luz de Baja Intensidad , Úlcera Péptica/radioterapia , Adolescente , Adulto , Formación de Anticuerpos/inmunología , Formación de Anticuerpos/efectos de la radiación , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neutrófilos/enzimología , Neutrófilos/efectos de la radiación , Úlcera Péptica/inmunología , Úlcera Péptica/terapia , Fagocitosis/efectos de la radiación , Resultado del TratamientoRESUMEN
The authors present a technique of treating chronic calculous pyelonephritis with laser radiation and sinusoidal modulated currents which promotes a complete elimination of the calculus fragments in 100, 70 and 50% of the patients in the stone size 0.2-0.5 cm, 0.5-0.7 cm and > 7 cm, respectively. This combined therapy had also antiinflammatory, and immunity-stimulating effects.
Asunto(s)
Terapia por Estimulación Eléctrica/métodos , Terapia por Láser , Pielonefritis/rehabilitación , Cálculos Urinarios/rehabilitación , Formación de Anticuerpos/efectos de la radiación , Enfermedad Crónica , Terapia Combinada , Electrólitos/sangre , Electrólitos/efectos de la radiación , Humanos , Inmunidad Celular/efectos de la radiación , Inmunoglobulinas/sangre , Inmunoglobulinas/efectos de la radiación , Pielonefritis/sangre , Pielonefritis/inmunología , Cálculos Urinarios/sangre , Cálculos Urinarios/inmunologíaRESUMEN
Microwave puncture (MP) effects were compared to effects of 4-component basic medication in 63 patients with gastric ulcer. The study was made of nonspecific defense factors, serum levels of IgA, IgM, IgG, gastric juice levels of IgA, pool structure of circulating lymphocytes (DR+, CD3+, CD22+, CD4+, CD8+), spontaneous and LPS-stimulated Ig-synthetic function of B-lymphocytes. Mean time of the ulcer healing in MP usage made up 14.6 +/- 1.6 days, in standard therapy--27.2 +/- 3.1 days (P < 0.001). Immunomodulating effect shown by MP consisted in activation of nonspecific defense factors and activation of immunoglobulin-synthetizing function of B-lymphocytes.
Asunto(s)
Microondas/uso terapéutico , Úlcera Gástrica/inmunología , Úlcera Gástrica/rehabilitación , Puntos de Acupuntura , Antiulcerosos/uso terapéutico , Formación de Anticuerpos/efectos de los fármacos , Formación de Anticuerpos/efectos de la radiación , Quimioterapia Combinada , Humanos , Inmunidad Celular/efectos de los fármacos , Inmunidad Celular/efectos de la radiación , Inmunidad Innata/efectos de los fármacos , Inmunidad Innata/efectos de la radiación , Úlcera Gástrica/tratamiento farmacológicoRESUMEN
Study of titers of specific antibacterial antibodies in the plasma by passive hemagglutination test and of the number of antibody-producing cells (APC) by local hemolysis test in patients with purulent diseases of soft tissues showed that both values increased after UV exposure of autoblood. This increase was more expressed after differentiated exposure of erythrocytic and leukocytic mass than after common UV exposure, which is explained by more complete exposure of leukocytes participating in immunological reactions. Study of APC during a UV session showed an increase in the count of these cells 6-8 h after exposure.
Asunto(s)
Infecciones Bacterianas/inmunología , Infecciones Bacterianas/terapia , Transfusión de Sangre Autóloga/métodos , Sangre/efectos de la radiación , Infecciones de los Tejidos Blandos/inmunología , Infecciones de los Tejidos Blandos/terapia , Terapia Ultravioleta/métodos , Adolescente , Adulto , Anciano , Anticuerpos Antibacterianos/sangre , Anticuerpos Antibacterianos/efectos de la radiación , Formación de Anticuerpos/efectos de la radiación , Especificidad de Anticuerpos/efectos de la radiación , Femenino , Humanos , Masculino , Persona de Mediana Edad , Factores de TiempoRESUMEN
The narrow-band UVB (TL01) lamp (311 nm emission) was developed for use in phototherapy, as an alternative to a broad-band UVB source and to photochemotherapy, both of which have significant side effects and carry a risk of carcinogenesis. This new lamp has proved to be particularly effective at clearing psoriasis. It is now acknowledged that the TL01 lamp is probably 2-3 times more carcinogenic per minimum erythema dose than broad-band UVB, but the cumulative dose required in therapy is considerably less than when using broad-band UVB sources. In terms of irradiation dose, the TL01 lamp is about 5-10-fold less potent than broad-band UVB for erythema induction, hyperplasia, oedema, sunburn cell formation and Langerhans cell depletion from skin. However, the broad-band UVB to TL01 potency ratio for cis-urocanic acid formation in the skin is approximately unity. In addition, the TL01 lamp, as used in phototherapy, has relatively more suppressive effects than broad-band UVB on systemic immune responses as judged by natural killer cell activity, lymphoproliferation and cytokine responses. However, the TL01 lamp is less effective at reducing epidermal antigen presentation, inducing dendritic cell migration to lymph nodes draining irradiated sites and suppressing contact hypersensitivity at the doses tested. Therefore the use of the TL01 lamp in phototherapy should be considered carefully after weighing up its diverse effects on the skin and immune system.
Asunto(s)
Rayos Ultravioleta , Terapia Ultravioleta/instrumentación , Animales , Formación de Anticuerpos/efectos de la radiación , Dermatitis por Contacto , Humanos , Inmunidad Celular/efectos de la radiación , Piel/efectos de la radiación , Neoplasias Cutáneas/etiología , Rayos Ultravioleta/efectos adversos , Terapia Ultravioleta/efectos adversosRESUMEN
Con A stimulated lymphocytes proliferation was measured as [3H]thymidine incorporation and IgG was quantified by single radial immunodiffusion to study recovering or protecting effect of selenium (Se) on immunity attacked by exogenous active oxygen species, H2O2 and 60Co-radiation, respectively. Lipid peroxidation was also determined to observe the relation between antioxidation ability and protecting ability of Se. It was found that H2O2 injured lymphocytes immunocompetence deeply and 60Co-radiation decreased immune response capacity greatly, but that administration of Se counteracts this damage. The antioxidative ability of Se was correlated with its protecting ability.
Asunto(s)
Antioxidantes/farmacología , Peróxido de Hidrógeno/antagonistas & inhibidores , Peróxido de Hidrógeno/toxicidad , Inmunidad/efectos de los fármacos , Inmunidad/efectos de la radiación , Selenio/farmacología , Animales , Formación de Anticuerpos/efectos de los fármacos , Formación de Anticuerpos/efectos de la radiación , Radioisótopos de Cobalto , Concanavalina A/farmacología , Rayos gamma , Inmunidad Celular/efectos de los fármacos , Inmunidad Celular/efectos de la radiación , Peróxidos Lipídicos/metabolismo , Ratones , Oxidantes/inmunología , Especies Reactivas de Oxígeno/inmunologíaRESUMEN
Among 33 patients with Stage II hypertension, 25 persons were inhabitants of the Russian radionuclide-contaminated areas and 8 patients had no prior contacts with ionizing radiation (control). All the patients examined were maintained within 4 weeks on a diet, radioprotective effect of which was realized due to the elevated content of sulfur containing amino acids, antioxidants and complexes. In the patients who lived in the radionuclide-contaminated areas, a moderate activation of lipid peroxidation was detected simultaneously with marked alterations in the humoral component of the immunity system, which involved a considerable increase in the levels of IgG and complements C3 and C4. A decrease in content of malonic dialdehyde and diene conjugates as well as normalization of IgG and decrease in content of IgA, IgM and C3 were observed in blood plasma of the patients on diet. Supplementation of omega 3 polyunsaturated fatty acids (omega 3-PUFA), 5 g/day and alpha-tocopherol, 12.5 mg/day, to the diet elevated the serum concentrations of all lg classes, which suggests the presence of an immunomodulating effect of omega 3-PUFA. Moreover, the content of malonic dialdehyde was not increased in blood plasma if 3-PUFA was used. Hence, increased alpha-tocopherol levels should be used in the treatment of these patients with omega 3-PUFA (up to 5-7 mg per g PUFA) in order to stabilize lipid peroxidation.
Asunto(s)
Contaminantes Radiactivos del Aire/toxicidad , Formación de Anticuerpos/efectos de la radiación , Peroxidación de Lípido/efectos de la radiación , Contaminantes Radiactivos del Suelo/toxicidad , Accidentes , Adyuvantes Inmunológicos/uso terapéutico , Adulto , Complemento C3/metabolismo , Complemento C4/metabolismo , Femenino , Humanos , Inmunoglobulina A/metabolismo , Inmunoglobulina G/metabolismo , Inmunoglobulina M/metabolismo , Masculino , Malondialdehído/metabolismo , Persona de Mediana Edad , Reactores Nucleares , UcraniaRESUMEN
A study is presented of the effect of reinfusions of ultraviolet-irradiated autologous blood on the number of lymphocytes, titer of circulating immune complexes and theophylline-sensitive cells, number of immunoglobulins A, G and M, number and form of erythrocytes in 30 patients with stable and progressive exertion stenocardia. Thirty control patients were treated with nitrates, calcium antagonists and acetylsalicylic acid. It was established that in patients with stenocardia quantum hemotherapy produces an immunocorrective action reduces the titer of circulating immune complexes, the content of T-lymphocytes, the number of immunoglobulins A and G, normalizes the morphological and functional activity of erythrocytes. Use of this method in the treatment of ischemic heart disease is pathogenetically justified and prevents development of myocardial infarction.
Asunto(s)
Transfusión de Sangre Autóloga/métodos , Sangre/efectos de la radiación , Eritrocitos/efectos de la radiación , Isquemia Miocárdica/terapia , Terapia Ultravioleta/métodos , Formación de Anticuerpos/efectos de la radiación , Enfermedad Crónica , Deformación Eritrocítica/efectos de la radiación , Eritrocitos/patología , Femenino , Humanos , Inmunidad Celular/efectos de la radiación , Masculino , Isquemia Miocárdica/sangre , Isquemia Miocárdica/inmunologíaRESUMEN
The authors have carried on an investigation of humoral immunity in 64 patients with thyrotoxicosis of different degrees. It was found that patients with different degrees of thyrotoxicosis had an elevated level of immunoglobulins and that autotransfusion of UV irradiated blood considerably normalized the content of immunoglobulins in blood serum, especially in patients with mild and mean degrees of thyrotoxicosis.
Asunto(s)
Transfusión de Sangre Autóloga , Sangre/efectos de la radiación , Tirotoxicosis/inmunología , Terapia Ultravioleta , Adolescente , Adulto , Formación de Anticuerpos/inmunología , Formación de Anticuerpos/efectos de la radiación , Terapia Combinada , Femenino , Humanos , Inmunoglobulinas/sangre , Inmunoglobulinas/efectos de la radiación , Masculino , Persona de Mediana Edad , Tirotoxicosis/terapiaRESUMEN
The levels of IgG, IgA and IgM were measured in the blood serum of uranium miners in a minin-district, where after a geological disturbance exposure to a high level of ionizing radiation took place. The mean exposure to 222-Rn daughters after first year was 7.35 WLM, after the second year 0.13 WLM only. Serum was sampled before miners started their occupation, for the second time after the first year and for the third time after the second year. The levels of IgG and IgM significantly decreased after one year of exposure, while the IgA changed only slightly. After the second year the levels of IgG and IgM significantly rose. IgG exceeded the initial levels: a regenerative hypercompensation took place. On the other hand IgA at the same time decreased. The IgG levels are only slightly correlated with radiation dose rate; for IgA and IgM the correlation is lacking. Individual Ig levels appear to be very variable. The reduction of IgG reached hypogammaglobulinemic levels in some miners after the first year and was followed by a propensity to infections of the respiratory tract. The significance of these observations is discussed from the immunological, radiological and radiohygienical point of view.