RESUMEN
The development of a new generation of biomaterials with high osteogenic ability for treatment of osteoporotic fractures is being intensively investigated. The objective of this paper was to investigate new bone formation in an ovariectomized rat (OVX rat) calvarial model of critical size bone defects filled with Sr-containing α-calcium sulfate hemihydrate (SrCSH) cement compared to an α-calcium sulfate hemihydrate (α-CSH) cement and empty defect. X-ray diffraction analysis verified the partial substitution of Sr2+ for Ca2+ did not change the phase composition of α-CSH. Scanning electron microscopy showed that Sr-substituted α-CSH significantly increased the surface roughness. The effects of Sr substitution on the biological properties of SrCSH cement were evaluated by adhesion, proliferation, alkaline phosphatase (ALP) activity of osteoblast-like cells MC3T3-E1. The results showed that SrCSHs enhanced MC3T3-E1 cell proliferation, differentiation, and ALP activity. Furthermore, SrCSH cement was used to repair critical-sized OVX rat calvarial defects. The in vivo results revealed that SrCSH had good osteogenic capability and stimulated new blood vessel formation in a critical sized OVX calvarial defect within 12 weeks, suggesting that SrCSH cement has more potential for application in bone tissue regeneration.
Asunto(s)
Sustitutos de Huesos/síntesis química , Sustitutos de Huesos/uso terapéutico , Sulfato de Calcio/química , Osteogénesis , Fracturas Osteoporóticas/terapia , Fracturas Craneales/terapia , Estroncio/química , Células 3T3 , Animales , Femenino , Ensayo de Materiales , Ratones , Fracturas Osteoporóticas/patología , Fracturas Osteoporóticas/fisiopatología , Ovariectomía , Ratas , Ratas Sprague-Dawley , Fracturas Craneales/patología , Fracturas Craneales/fisiopatología , Resultado del TratamientoAsunto(s)
Hueso Nasal/lesiones , Nariz/lesiones , Accidentes de Tránsito , Intoxicación Alcohólica/complicaciones , Anestesia Local , Rinorrea de Líquido Cefalorraquídeo/patología , Niño , Contusiones/etiología , Equimosis/patología , Edema/patología , Epistaxis/patología , Hueso Frontal/lesiones , Hematoma/patología , Humanos , Tabique Nasal/lesiones , Fracturas Orbitales/patología , Fracturas Craneales/etiología , Fracturas Craneales/patología , Fracturas Craneales/fisiopatología , Fracturas Craneales/terapia , ViolenciaRESUMEN
PURPOSE: This study evaluated the effect of insulin on fracture in fetal rat intramembranous bone using an organ culture system in which bone mineralizes in vitro. MATERIALS AND METHODS: Twenty-day-old fetal rat parietal bones were fractured parallel to the frontal plane and cultured in a serum-free medium with or without insulin for 4 to 12 days. They were then processed for light microscopy. RESULTS: In the specimens not treated with insulin, bony bridges were observed in less than 10% on day 4 and 8, and only 45% on day 12. In bones treated with insulin at 10(-6) mol/L, bony bridges were observed in 73% on day 4 and 100% on day 8 and 12. No callus or cartilage formation was observed during the wound repair process, indicating that intramembranous ossification occurred. CONCLUSION: These findings suggest that insulin has a direct effect on bone cells, which enhances the healing of fractures in intramembranous bone.