RESUMEN
In the present study, the effect of alcoholic stem extract of Gymnema montanum (GMSt) on blood glucose, plasma insulin, and carbohydrate metabolic enzymes were studied in experimental diabetes. Diabetes mellitus was induced by a single intraperitoneal injection of STZ (60 mg/kg bw). Five days after STZ induction, diabetic rats received GMSt orally at the doses of 25, 50, 100 and 200mg/kg daily for 3 weeks. Graded doses of stem extract showed a significant reduction in blood glucose levels and improvement in plasma insulin levels. The effect was more pronounced in 100 and 200mg/kg than 50mg/kg. GMSt showed significant increase in hexokinase, Glucose-6-phosphate dehydrogenase and glycogen content in liver of diabetic rats while there was significant reduction in the levels of glucose-6-phosphatase and fructose-1,6-bisphosphatase. The present study clearly indicated significant antidiabetic effect with the stem extract of G. montanum and lends support for its traditional usage.
Asunto(s)
Diabetes Mellitus Experimental/tratamiento farmacológico , Gymnema/química , Hipoglucemiantes/administración & dosificación , Fitoterapia , Extractos Vegetales/administración & dosificación , Animales , Glucemia/efectos de los fármacos , Diabetes Mellitus Experimental/patología , Relación Dosis-Respuesta a Droga , Fructosa-Bifosfatasa/análisis , Fructosa-Bifosfatasa/efectos de los fármacos , Fructosa-Bifosfatasa/metabolismo , Glucosa-6-Fosfatasa/análisis , Glucosa-6-Fosfatasa/efectos de los fármacos , Glucosa-6-Fosfatasa/metabolismo , Glucosafosfato Deshidrogenasa/análisis , Glucosafosfato Deshidrogenasa/efectos de los fármacos , Glucosafosfato Deshidrogenasa/metabolismo , Glucógeno/análisis , Glucógeno/metabolismo , Insulina/sangre , Hígado/efectos de los fármacos , Hígado/enzimología , Masculino , Hojas de la Planta/química , Tallos de la Planta/química , Plantas Medicinales , Ratas , Ratas WistarRESUMEN
High intake of dietary fructose has been shown to exert a number of adverse metabolic eff ects in humans and experimental animals. The present study was designed to investigate the eff ect of the aqueous extract of Tinospora cordifolia stem (TCAE) on the adverse eff ects of fructose loading toward carbohydrate and lipid metabolism in rats. Adult male Wistar rats of body weight around 200 g were divided into four groups, two of which were fed with starch diet and the other two with high fructose (66 %) diet. Plant extract of TC (400 mg/kg/day) was administered orally to each group of the starch fed rats and the highfructose fed rats. At the end of 60 days of experimental period, biochemical parameters related to carbohydrate and lipid metabolism were assayed. Hyperglycemia, hyperinsulinemia, hypertriglyceridemia, insulin resistance, and elevated levels of hepatic total lipids, cholesterol, triglycerides, and free fatty acids (p < 0.05) observed in fructose-fed rats were completely prevented with TCAE treatment. Alterations in the activities of enzymes of glucose metabolism (hexokinase, phosphofructokinase, pyruvate kinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, and glucose-6-phosphate dehydrogenase) and lipid metabolism (fatty acid synthetase, lipoprotein lipase, and malic enzyme) as observed in the high fructose-fed rats were prevented with TCAE administration. In conclusion, our fi ndings indicate improvement of glucose and lipid metabolism in high-fructose fed rats by treatment with Tinospora cordifolia, and suggest that the plant can be used as an adjuvant for the prevention and/or management of insulin resistance and disorders related to it.