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Métodos Terapéuticos y Terapias MTCI
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1.
Carbohydr Polym ; 267: 118172, 2021 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-34119144

RESUMEN

The fruit of Lycium ruthenicum Murr is used as traditional medicine and functional food. Previously we reported that one RG-I pectin from this fruit might inhibit pancreatic cancer cells growth. We further hypothesized that there might be other type of polysaccharides in this fruit also have anti-tumor effect. Here, we showed novel structure of a homogeneous polysaccharide named LRP1-S2 from this fruit and its anti-pancreatic cancer effect. Structure analyses suggested that LRP1-S2 was a novel arabinogalactan with the molecular weight (Mw) of 17.0 kDa. Bioactivity test showed that LRP1-S2 might attenuate the proliferation of pancreatic cancer cells in vitro and in vivo without significant cytotoxicity to normal pancreatic HPDE6-C7 cells and LO2 liver cells. Mechanism study indicated that it might induce apoptosis of BxPC-3 by inactivating P38 MAPK/NF-κB and GSK-3ß/ß-Catenin signaling pathways. These results suggested that LRP1-S2 could be a potential anti-tumor leading compound for functional food and new drug development. CHEMICAL COMPOUNDS: arabinogalactan, pectin, galactan, arabinan, RN-1, HH1-1, LRP1-S2, LRP3-S1.


Asunto(s)
Antineoplásicos/uso terapéutico , Galactanos/uso terapéutico , Neoplasias Pancreáticas/tratamiento farmacológico , Animales , Antineoplásicos/química , Antineoplásicos/aislamiento & purificación , Antineoplásicos/toxicidad , Apoptosis/efectos de los fármacos , Secuencia de Carbohidratos , Línea Celular , Proliferación Celular/efectos de los fármacos , Frutas/química , Galactanos/química , Galactanos/aislamiento & purificación , Galactanos/toxicidad , Humanos , Lycium/química , Ratones Endogámicos BALB C , Ratones Desnudos , Transducción de Señal/efectos de los fármacos , Ensayos Antitumor por Modelo de Xenoinjerto
2.
Carbohydr Polym ; 206: 362-370, 2019 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-30553333

RESUMEN

A smart wound dressing based on carrageenan (κC), locust bean gum (LBG), and cranberry extract (CB) for monitoring bacterial wound infections was developed and characterized using UV-vis spectroscopy, FT-IR, and SEM. The mechanical, swelling, cytotoxic and pH sensor properties were also investigated. UV-vis spectra demonstrated that the obtained κC:LBG:CB hydrogel film exhibited a visible change of colors as it was immersed in PBS solution pH 5.0, 7.3 and 9.0. The spectra of FT-IR suggested that chemical interactions had occurred between κC and CB extract. The obtained κC:LBG:CB hydrogel film exhibited adequate mechanical properties and a swelling behavior dependent on pH. Cytotoxicity tests indicated that κC:LBG:CB hydrogel film had dose-dependent cytotoxicity against NIH 3T3 fibroblast cells. The in vitro studies using Staphylococcus aureus and Pseudomonas aeruginosa demonstrated that the color changes of the κC:LBG:CB hydrogel film could be observed by naked eyes, confirming the potential use of the obtained hydrogel film as a visual system for monitoring bacterial wound infections.


Asunto(s)
Infecciones Bacterianas/diagnóstico , Vendajes , Hidrogeles/química , Indicadores y Reactivos/farmacología , Extractos Vegetales/farmacología , Infección de Heridas/diagnóstico , Animales , Antocianinas/química , Antocianinas/farmacología , Antocianinas/toxicidad , Antibacterianos/química , Antibacterianos/farmacología , Antibacterianos/toxicidad , Carragenina/química , Carragenina/toxicidad , Color , Módulo de Elasticidad , Galactanos/química , Galactanos/toxicidad , Hidrogeles/toxicidad , Concentración de Iones de Hidrógeno , Indicadores y Reactivos/química , Indicadores y Reactivos/toxicidad , Mananos/química , Mananos/toxicidad , Ratones , Células 3T3 NIH , Extractos Vegetales/química , Extractos Vegetales/toxicidad , Gomas de Plantas/química , Gomas de Plantas/toxicidad , Pseudomonas aeruginosa/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacos , Resistencia a la Tracción , Vaccinium macrocarpon/química
3.
Fitoterapia ; 81(4): 276-83, 2010 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-19825399

RESUMEN

A purified Arabinogalactan-Protein composition (LL-4218) was prepared from the leaves of Argemone mexicana to treat psoriasis. The effect of (LL-4218) was evaluated on reproductive (male and female fertility) and developmental toxicity in rats. LL-4218 was administered orally at the doses of 250, 500 and 1000 mg kg(-1). The results showed that LL-4218 did not produce any significant dose related changes in reproductive and developmental toxicity studies. Therefore, it is concluded that LL-4218 did not produce any significant toxic effect on reproduction and developmental parameters of rats and NOAEL for reproductive and developmental toxicity studies in rats was 1000 mg kg(-1).


Asunto(s)
Argemone/química , Fertilidad/efectos de los fármacos , Desarrollo Fetal/efectos de los fármacos , Galactanos/toxicidad , Extractos Vegetales/toxicidad , Proteínas de Plantas/toxicidad , Animales , Femenino , Galactanos/aislamiento & purificación , Masculino , Extractos Vegetales/química , Proteínas de Plantas/aislamiento & purificación , Embarazo , Ratas , Ratas Wistar
4.
Carbohydr Res ; 308(3-4): 349-57, 1998 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-9711828

RESUMEN

The two main exocellular polysaccharides produced in vitro by Phomopsis foeniculi, a fungal pathogen of fennel, were isolated and characterized by chemical and spectroscopic methods as a galactan with the known structure [-->6)-beta-D-Galf-(1-->5)-beta-D- Galf-(1-->5)-beta-D-Galf-(1-->]n and a mannan. The latter consists of a backbone of alpha-(1-->6)-linked mannopyranose units. Almost all of these are branched at the 2 position with arms containing 2- and 3-linked mannopyranose units. The crude polysaccharide fraction and its components, galactan and mannan, showed phytotoxic effects, i.e. chlorosis, necrosis and/or wilting, on fennel and on two non-host plants, tobacco and tomato.


Asunto(s)
Ascomicetos/química , Micotoxinas/química , Plantas/efectos de los fármacos , Polisacáridos/química , Bioensayo , Secuencia de Carbohidratos , Ferula/parasitología , Galactanos/química , Galactanos/toxicidad , Mananos/química , Mananos/toxicidad , Datos de Secuencia Molecular , Micotoxinas/toxicidad , Plantas Medicinales , Plantas Tóxicas , Polisacáridos/toxicidad , Pruebas de Toxicidad
5.
Am J Clin Nutr ; 41(5): 891-4, 1985 May.
Artículo en Inglés | MEDLINE | ID: mdl-2986444

RESUMEN

The use of diets rich in unabsorbable carbohydrate ("fiber") has been advocated for the treatment of noninsulin-dependent diabetes mellitus (NIDDM). The soluble viscous fibers such as guar gum are most effective in normalizing carbohydrate intolerance in such patients; particulate fibers such as cellulose have little or no effect. While the latter are known to affect many aspects of nutrition when consumed in great quantity, little is known of the toxicity of guar gum. Eight adults with NIDDM are reported here who consumed at least 30 grams of guar gum for at least 16 weeks without any change in hematologic, hepatic, or renal function. Serologic screening revealed no change in lipid, protein or mineral metabolism, and no change in electrolyte balance. It is concluded that consumption of 30 grams of guar gum per day for prolonged periods is without serious consequences.


Asunto(s)
Diabetes Mellitus Tipo 2/dietoterapia , Fibras de la Dieta/toxicidad , Galactanos/toxicidad , Mananos/toxicidad , Células Sanguíneas/efectos de los fármacos , Coagulación Sanguínea/efectos de los fármacos , Diabetes Mellitus Tipo 2/sangre , Fibras de la Dieta/uso terapéutico , Femenino , Alimentos Fortificados , Galactanos/uso terapéutico , Humanos , Masculino , Mananos/uso terapéutico , Persona de Mediana Edad , Gomas de Plantas , Estudios Prospectivos , Factores de Tiempo
6.
Food Chem Toxicol ; 22(6): 415-8, 1984 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-6539730

RESUMEN

Agar, carboxymethylcellulose, carrageenan, guar gum, gum acacia, locust-beam gum or pectin (50 g/kg diet), given to weanling rats for 4 wk, increased the weight of the caecal wall and the caecal contents. Feeding carboxymethylcellulose, guar gum or pectin significantly increased, and feeding carrageenan decreased, the total bacterial population of the caecum. Feeding carboxymethylcellulose significantly increased in vitro activity of bacterial azoreductase, beta-glucosidase, beta-glucuronidase, nitrate reductase, nitroreductase and urease. Guar gum, gum acacia and locust-bean gum each increased at least three of these activities. In contrast, feeding carrageenan greatly decreased all microbial enzyme activities, while agar decreased beta-glucosidase, beta-glucuronidase and nitroreductase activities.


Asunto(s)
Ciego/microbiología , Coloides/toxicidad , Aditivos Alimentarios/toxicidad , Agar/toxicidad , Animales , Carboximetilcelulosa de Sodio/toxicidad , Carragenina/toxicidad , Ciego/efectos de los fármacos , Ciego/enzimología , Galactanos/toxicidad , Goma Arábiga/toxicidad , Masculino , Mananos/toxicidad , Pectinas/toxicidad , Gomas de Plantas , Polisacáridos/toxicidad , Ratas , Ratas Endogámicas
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