RESUMEN
Activities of succinate dehydrogenase (SDH) and alpha-glycerophosphate dehydrogenase (hyaloplasmic and mitochondrial: alpha-GPDHH and alpha-GPDHM) in peripheral blood lymphocytes, and the response of SDH activity to exogenous epinephrine in vitro (the epinephrine test) were studied in 20 healthy subjects and 46 patients with hypertensive neurocirculatory dystonia. Activities of SDH, alpha-GPDHH, and alpha-GPDHM in blood lymphocytes and SDH adrenoreactivity in epinephrine test were higher in patients than in healthy controls. Treatment with hypotensive agents (isradipin and pyrroxan) moderated adrenoreactivity. Phytotherapy normalized the baseline activities of succinate oxidase system and alpha-glycerophosphate pathway in blood lymphocytes.
Asunto(s)
Epinefrina/farmacología , Leucocitos Mononucleares/enzimología , Mitocondrias/enzimología , Astenia Neurocirculatoria/enzimología , Oxidorreductasas/sangre , Adulto , Antihipertensivos/farmacología , Células Cultivadas , Dioxanos/farmacología , Relación Dosis-Respuesta a Droga , Activación Enzimática , Glicerolfosfato Deshidrogenasa/sangre , Humanos , Isradipino/farmacología , Leucocitos Mononucleares/efectos de los fármacos , Leucocitos Mononucleares/patología , Masculino , Succinato Deshidrogenasa/sangre , Factores de TiempoAsunto(s)
Linfocitos/enzimología , Úlcera Péptica/enzimología , Úlcera Péptica/terapia , Adulto , Femenino , Glicerolfosfato Deshidrogenasa/sangre , Antagonistas de los Receptores H2 de la Histamina/uso terapéutico , Humanos , Oxigenoterapia Hiperbárica , Masculino , Persona de Mediana Edad , Succinato Deshidrogenasa/sangreRESUMEN
Selenium (Se) deficiency for 5 weeks in rats produced changes in the activity of a number of hepatic, renal and plasma enzymes. In animals whose food intake was restricted to 75% of normal for 2 weeks, Se deficiency produced significant increases in the activity of hepatic cytosolic 'malic' enzyme and mitochondrial alpha-glycerophosphate dehydrogenase (GPD), two enzymes that are particular sensitive to the thyroid-hormone concentrations in tissue. Propylthiouracil-induced hypothyroidism produced significant decreases in 'malic' enzyme and GPD activities. The effect of hypothyroidism on the activity of 'malic' enzyme, GPD and other enzymes studied in liver and plasma was often opposite to that seen in Se deficiency. Glutathione S-transferase (GST) activity was increased by both Se deficiency and hypothyroidism, but in hypothyroid animals further significant increases in GST were produced by Se deficiency. These data suggest that the changes in enzyme expression observed in Se deficiency are not caused by decreased tissue exposure to thyroid hormones.
Asunto(s)
Hipotiroidismo/enzimología , Mitocondrias Hepáticas/enzimología , Oxidorreductasas/metabolismo , Selenio/deficiencia , Animales , Glutatión Peroxidasa/sangre , Glutatión Peroxidasa/metabolismo , Glicerolfosfato Deshidrogenasa/sangre , Glicerolfosfato Deshidrogenasa/metabolismo , Riñón/enzimología , Malato Deshidrogenasa/sangre , Malato Deshidrogenasa/metabolismo , Masculino , Tamaño de los Órganos , Oxidorreductasas/sangre , Propiltiouracilo , Ratas , Glándula Tiroides/enzimología , Tiroxina/sangre , Tiroxina/metabolismo , Triyodotironina/sangre , Triyodotironina/metabolismoRESUMEN
Plant polysaccharide palyustran inhibited the growth of human lung carcinoma P-1 transplanted to athymic mice by 60% but failed to do so in human rhabdomyosarcoma. Treatment with palyustran was followed by a 2-fold decrease in lympho- and granulocyte levels, selective inhibition of succinate dehydrogenase and alpha-glycerophosphate dehydrogenase activity in tumor cells and--in single cases--metastatic involvement of the liver.