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1.
Mol Neurobiol ; 57(5): 2206-2219, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-31981074

RESUMEN

Prion diseases are fatal infectious neurodegenerative disorders in human and animals caused by misfolding of the cellular prion protein (PrPC) into the infectious isoform PrPSc. These diseases have the potential to transmit within or between species, and no cure is available to date. Targeting the unfolded protein response (UPR) as an anti-prion therapeutic approach has been widely reported for prion diseases. Here, we describe the anti-prion effect of the chemical compound Sephin1 which has been shown to protect in mouse models of protein misfolding diseases including amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) by selectively inhibiting the stress-induced regulatory subunit of protein phosphatase 1, thus prolonging eIF2α phosphorylation. We show here that Sephin1 dose and time dependently reduced PrPSc in different neuronal cell lines which were persistently infected with various prion strains. In addition, prion seeding activity was reduced in Sephin1-treated cells. Importantly, we found that Sephin1 significantly overcame the endoplasmic reticulum (ER) stress induced in treated cells, as measured by lower expression of stress-induced aberrant prion protein. In a mouse model of prion infection, intraperitoneal treatment with Sephin1 significantly prolonged survival of prion-infected mice. When combining Sephin1 with the neuroprotective drug metformin, the survival of prion-infected mice was also prolonged. These results suggest that Sephin1 could be a potential anti-prion drug selectively targeting one component of the UPR pathway.


Asunto(s)
Guanabenzo/análogos & derivados , Proteínas PrPC/metabolismo , Proteínas PrPSc/metabolismo , Priones/efectos de los fármacos , Scrapie/tratamiento farmacológico , Respuesta de Proteína Desplegada/efectos de los fármacos , Animales , Línea Celular Tumoral , Evaluación Preclínica de Medicamentos , Sinergismo Farmacológico , Estrés del Retículo Endoplásmico/efectos de los fármacos , Factor 2 Eucariótico de Iniciación/metabolismo , Guanabenzo/administración & dosificación , Guanabenzo/farmacología , Guanabenzo/uso terapéutico , Metformina/administración & dosificación , Metformina/farmacología , Metformina/uso terapéutico , Ratones , Neuroblastoma/patología , Fármacos Neuroprotectores/administración & dosificación , Fármacos Neuroprotectores/farmacología , Fármacos Neuroprotectores/uso terapéutico , Fosforilación/efectos de los fármacos , Proteína Fosfatasa 1/antagonistas & inhibidores , Procesamiento Proteico-Postraduccional/efectos de los fármacos , Proteínas Serina-Treonina Quinasas/metabolismo , Scrapie/patología
2.
J Clin Invest ; 123(12): 5119-34, 2013 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-24231350

RESUMEN

A systems pharmacological approach that capitalizes on the characterization of intracellular signaling networks can transform our understanding of human diseases and lead to therapy development. Here, we applied this strategy to identify pharmacological targets for the treatment of Stargardt disease, a severe juvenile form of macular degeneration. Diverse GPCRs have previously been implicated in neuronal cell survival, and crosstalk between GPCR signaling pathways represents an unexplored avenue for pharmacological intervention. We focused on this receptor family for potential therapeutic interventions in macular disease. Complete transcriptomes of mouse and human samples were analyzed to assess the expression of GPCRs in the retina. Focusing on adrenergic (AR) and serotonin (5-HT) receptors, we found that adrenoceptor α 2C (Adra2c) and serotonin receptor 2a (Htr2a) were the most highly expressed. Using a mouse model of Stargardt disease, we found that pharmacological interventions that targeted both GPCR signaling pathways and adenylate cyclases (ACs) improved photoreceptor cell survival, preserved photoreceptor function, and attenuated the accumulation of pathological fluorescent deposits in the retina. These findings demonstrate a strategy for the identification of new drug candidates and FDA-approved drugs for the treatment of monogenic and complex diseases.


Asunto(s)
Inhibidores de Adenilato Ciclasa , Agonistas alfa-Adrenérgicos/uso terapéutico , Antagonistas Adrenérgicos alfa/uso terapéutico , Degeneración Macular/congénito , Terapia Molecular Dirigida , Proteínas del Tejido Nervioso/biosíntesis , Células Fotorreceptoras de Vertebrados/efectos de los fármacos , Receptor de Serotonina 5-HT2A/biosíntesis , Receptores Adrenérgicos alfa 2/biosíntesis , Antagonistas de la Serotonina/uso terapéutico , Transportadoras de Casetes de Unión a ATP/deficiencia , Transportadoras de Casetes de Unión a ATP/genética , Adenina/análogos & derivados , Adenina/farmacología , Adenina/uso terapéutico , Agonistas alfa-Adrenérgicos/farmacología , Antagonistas Adrenérgicos alfa/farmacología , Oxidorreductasas de Alcohol/deficiencia , Oxidorreductasas de Alcohol/genética , Animales , Supervivencia Celular , Modelos Animales de Enfermedad , Doxazosina/farmacología , Doxazosina/uso terapéutico , Evaluación Preclínica de Medicamentos , Guanabenzo/farmacología , Guanabenzo/uso terapéutico , Humanos , Luz/efectos adversos , Macaca fascicularis , Degeneración Macular/tratamiento farmacológico , Degeneración Macular/genética , Degeneración Macular/prevención & control , Ratones , Ratones Endogámicos BALB C , Ratones Noqueados , Proteínas del Tejido Nervioso/genética , Células Fotorreceptoras de Vertebrados/patología , Células Fotorreceptoras de Vertebrados/fisiología , Células Fotorreceptoras de Vertebrados/efectos de la radiación , Especies Reactivas de Oxígeno , Receptor de Serotonina 5-HT2A/genética , Receptores Adrenérgicos alfa 2/genética , Receptores Acoplados a Proteínas G/biosíntesis , Receptores Acoplados a Proteínas G/genética , Antagonistas de la Serotonina/farmacología , Transducción de Señal , Enfermedad de Stargardt
3.
J Clin Hypertens ; 3(4): 397-404, 1987 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-3453382

RESUMEN

Sodium retention may partially offset the therapeutic action of some antihypertensive agents. To assess the effects of guanabenz on sodium balance, six men with mild to moderate hypertension were placed on diets with constant sodium intake (120 mEq/day) for approximately 4 weeks. After achieving sodium balance, the subjects received guanabenz (16-24 mg daily) for approximately 2 weeks. Mean supine blood pressure decreased from 144/93 to 133/86 mmHg during guanabenz treatment (p less than 0.001). Guanabenz therapy was associated with a decrease in body weight (mean +/- SE) from 85.4 +/- 7.0 to 84.4 +/- 6.8 kg (p less than 0.01). Sodium balance, glomerular filtration rate, plasma renin activity, mean maximal urine osmolality, fluid intake, urine volume, and serum sodium concentration were unchanged during guanabenz therapy. Three additional balance studies were performed during a period of greater sodium intake (180 mEq/day). Although higher doses of guanabenz were required to achieve blood pressure control, sodium balance still was not affected by the drug. Thus, an effective therapeutic dose of guanabenz administered for 2 weeks had no clinically significant effects on sodium or water homeostasis in patients with mild to moderate hypertension.


Asunto(s)
Guanabenzo/uso terapéutico , Guanidinas/uso terapéutico , Hipertensión/tratamiento farmacológico , Equilibrio Hidroelectrolítico/efectos de los fármacos , Adulto , Presión Sanguínea/efectos de los fármacos , Creatinina/sangre , Humanos , Capacidad de Concentración Renal/efectos de los fármacos , Masculino , Persona de Mediana Edad , Sodio en la Dieta/administración & dosificación
5.
Artículo en Inglés | MEDLINE | ID: mdl-6679138

RESUMEN

I should like to preface my concluding remarks by noting that our observations are still of a preliminary nature. Guanabenz, an acute central adrenergic inhibitor produces in human subjects (previously volume expanded with saline) changes similar to those previously described in animals, namely a rise or no change in GFR, an enhancement of water diuresis, and a significant natriuresis. Within one week of the acute study and during chronic administration of guanabenz, all of these changes were reversed: GFR, the magnitude of the water diuresis and the enhanced sodium excretion had returned to the baseline pre-guanabenz levels, indicating that certain adaptive, restorative mechanism were operative. One may conclude, however, that the potential natriuretic properties of guanabenz (even during chronic administration), counter-balance the sodium retaining side effects commonly seen with other centrally acting and vasodilating antihypertensive drugs. This property, therefore, makes this agent a scientifically interesting and potentially useful therapeutic drug in the treatment of high blood pressure.


Asunto(s)
Diuresis/efectos de los fármacos , Guanabenzo/uso terapéutico , Guanidinas/uso terapéutico , Natriuresis/efectos de los fármacos , Adulto , Evaluación de Medicamentos , Hemodinámica/efectos de los fármacos , Humanos , Hipertensión/tratamiento farmacológico , Hipertensión/fisiopatología , Riñón/efectos de los fármacos , Riñón/fisiopatología , Masculino , Persona de Mediana Edad , Factores de Tiempo
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