RESUMEN
Hedyotis diffusa Willd. (Rubiaceae) is an important folk herb used to prevent and cure hepatitis and liver cancer in Taiwan. For differentiation of H. diffusa from counterfeits, macroscopic and microscopic characters of H. diffusa, H. corymbosa and H. tenelliflora were examined in this study. According to Trypan blue exclusion assay and Western blot analysis, H. diffusa had a significant inhibition of cell growth and induction of cell apoptosis in COLO 205 (colon cancer), Hep 3B (hepatocellular carcinoma) and H460 (lung cancer) cell lines. This study also used high-performance liquid chromatography (HPLC) to determine the quality control of H. diffusa. The HPLC data showed that ursolic and oleanolic acid are the components of the H. diffusa, consisting of approximately 4.66-4.80% and 1.86-1.96%, respectively. Our study also demonstrated that ursolic acid has significant anti-tumor activity in COLO 205, Hep 3B and H460 cancer cells.
Asunto(s)
Antineoplásicos Fitogénicos/uso terapéutico , Contaminación de Medicamentos/prevención & control , Hedyotis/química , Neoplasias/tratamiento farmacológico , Fitoterapia , Extractos Vegetales/uso terapéutico , Triterpenos/uso terapéutico , Antineoplásicos Fitogénicos/análisis , Antineoplásicos Fitogénicos/farmacología , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Hedyotis/clasificación , Humanos , Ácido Oleanólico/análisis , Fenotipo , Extractos Vegetales/química , Extractos Vegetales/farmacología , Control de Calidad , Especificidad de la Especie , Triterpenos/análisis , Triterpenos/farmacología , Ácido UrsólicoRESUMEN
An HPLC-DAD-ESI-MS/MS method was developed for analysis of iridoid glucosides (IGs) from Hedyotis diffusa Willd. The optimized separation condition was achieved with the Complex Sample Analysis Software System (CSASS) software, under which the whole analytes were achieved complete resolution especially for some isomeric IGs. Based on the UV and fragmentations, eleven IGs were detected. According to the fragmentation patterns of the three standard IGs, especially those of the isomeric standards, seven IGs including three pairs of isomers were unambiguous/tentatively identified. For the isomeric IGs with methyl ester or carboxyl group at C-4, the extents of the losses of CH3OH and/or H2O from their molecular and/or the aglycone adducts are useful for the differentiation of the stereoisomers in positive ion (PI) mode, which depends on the stereochemistry of the hydroxyl group on the cyclopentanoid unit.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Hedyotis/química , Hedyotis/clasificación , Iridoides/análisis , Espectrometría de Masa por Ionización de Electrospray/métodos , Espectrometría de Masas en Tándem/métodos , Iridoides/química , Estructura Molecular , Componentes Aéreos de las Plantas/química , Extractos Vegetales/análisis , Extractos Vegetales/química , Polvos , Estándares de Referencia , Reproducibilidad de los Resultados , Programas Informáticos , Espectrofotometría Ultravioleta/métodos , EstereoisomerismoRESUMEN
OBJECTIVE: To establish a new identification and analysis method of Herba Hedyotidis. METHOD: HPLC fingerprint pattern method. RESULT: Experiment and analysis were carried out on seven samples, the standard HPLC fingerprint pattern method and characterisitic diffraction peaks of Herba Hedyotidis were obtained. CONCLUSION: This method can be used for the identification of Herba Hedyotidis.