RESUMEN
Presently, a combination of chemotherapy, radiotherapy, thermotherapy, and other treatments has become a hot topic of research for the treatment of cancer, especially lung cancer. In this study, novel hollow gold nanoparticles (HGNPs) were used as drug carriers, and in order to improve the targeting ability of HGNPs to a lung tumor site, polyoxyethylene sorbitol oleate (PSO) was chosen here as a target ligand since it can be specifically recognized by the low-density lipoprotein (LDL) receptor which is usually over expressed on A549 lung cancer cells. In this way, a PSO-modified doxorubicin-loaded HGNP drug delivery system (PSO-HGNPs-DOX) was constructed and its physicochemical properties, photothermal conversion ability, and drug release of PSO-HGNPs-DOX was investigated. Further, the effects of triple combination therapy, the intracellular uptake, and the ability to escape macrophage phagocytosis of PSO-HGNPs-DOX were also studied using A549 cells in vitro. In addition, an in vivo mouse model was also used to study the targeting of PSO-HGNPs-DOX to lung cancer. PSO-HGNPs-DOX demonstrated a good triple therapeutic effect for lung cancer (A549 cell viability was only 10% at 500 µM) by LDL receptor mediated endocytosis and was able to escape macrophage phagocytosis to enhance its accumulation at the target site. Therefore, PSO-HGNPs-DOX is a novel, safe, promising, and targeted drug carrier designed for triple combination lung cancer therapy which should be further studied for such applications.
Asunto(s)
Endocitosis/fisiología , Hexosas/administración & dosificación , Neoplasias Pulmonares/metabolismo , Macrófagos/metabolismo , Fagocitosis/fisiología , Receptores de LDL/metabolismo , Células A549 , Animales , Células Cultivadas , Relación Dosis-Respuesta a Droga , Endocitosis/efectos de los fármacos , Oro/administración & dosificación , Humanos , Neoplasias Pulmonares/terapia , Macrófagos/efectos de los fármacos , Nanopartículas del Metal/administración & dosificación , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Fagocitosis/efectos de los fármacos , Terapia Fototérmica/métodos , Polietilenglicoles/administración & dosificación , Ratas , Receptores de LDL/administración & dosificación , Ensayos Antitumor por Modelo de Xenoinjerto/métodosRESUMEN
The impact of nanomedicine has grown in the current decade; however, only very few clinical translational attempts have been realized. Therefore in the present study, we hypothesized that bergamot oil, a psoralen-containing oil, would produce an optimized melanogenic effect in the clinical treatment of vitiligo when loaded within an elastic nanocarrier (spanlastics) and combined with PUVB for activation of psoralens. Spanlastics were prepared and characterized for particle size, physical stability, in vitro release, thermal behavior, deformability, morphology, and in vitro photostability. The efficacy of the selected formula was tested histopathologically on rat skin and clinically translated in patients suffering from vitiligo. Results revealed that the spanlastics were of reasonable nanosize, deformable, and provided sustained release of bergamot oil. The incorporation of bergamot oil within spanlastics improved its photostability and its photodynamic activity. Spanlastics exhibited promising clinical results in terms of extent and onset of repigmentation in vitiligo patients. Therefore, it can be concluded that spanlastics can be introduced as a promising nanotreatment modality for vitiligo.
Asunto(s)
Portadores de Fármacos/administración & dosificación , Nanoestructuras/administración & dosificación , Aceites de Plantas/administración & dosificación , Terapia Ultravioleta , Vitíligo/terapia , Adulto , Animales , Niño , Portadores de Fármacos/química , Liberación de Fármacos , Hexosas/administración & dosificación , Hexosas/química , Humanos , Nanoestructuras/química , Aceites de Plantas/química , Ratas , Resultado del TratamientoRESUMEN
Synbiotics, a combination of prebiotics and probiotics, produce synergistic effects to promote gastrointestinal health. Herein, we investigated the synbiotic interaction between the Lactobacillus rhamnosus strain GG (LGG; a probiotic strain) and tagatose (a prebiotic) in a dextran sulfate sodium (DSS)-induced colitis murine model. Initially, body weight, food intake, and clinical features were dramatically decreased after treatment with DSS, and the addition of LGG, tagatose, or both ameliorated these effects. In our pyrosequencing analysis of fecal microbiota, DSS treatment increased the abundance of Proteobacteria and decreased that of Firmicutes. When LGG and tagatose were administered as synbiotics, the gut microbiota composition recovered from the dysbiosis caused by DSS treatment. In particular, the abundance of Bacteroides, Lactobacillus, and Akkermansia was significantly associated with probiotic, prebiotic, and synbiotic treatments. Taken together, our results suggest that LGG and tagatose as synbiotics can alleviate colitis, and synbiotics could be applied as dietary supplements in dairy foods such as yogurt and cheese.
Asunto(s)
Colitis/inducido químicamente , Colitis/terapia , Hexosas/uso terapéutico , Lacticaseibacillus rhamnosus , Simbióticos , Animales , Sulfato de Dextran/toxicidad , Heces/microbiología , Hexosas/administración & dosificación , Quelantes del Hierro/administración & dosificación , Quelantes del Hierro/farmacología , Lactobacillus , Lacticaseibacillus rhamnosus/clasificación , Ratones , MicrobiotaRESUMEN
Nowadays, million women live with the human immunodeficiency virus (HIV) worldwide and many of them are dying per year, particularly in Sub-Saharan Africa. The development of systems that can be accessed by this population group to prevent the sexual transmission of the virus is therefore necessary. The aim of this work was the formulation of freeze-dried bioadhesive vaginal bigels releasing Tenofovir in a controlled manner. Systems containing three different proportions of guar gum hydrogel and sesame oil were prepared, adding Span®60 or Span®60 and Tween®60 as surfactants. Drug and excipients were evaluated by cytotoxicity assays, showing no toxicity at the concentrations tested neither for the drug nor any of the excipients. Fresh formulations were characterised through texture analyses and confocal laser microcopy. The system with the lowest guar gum hydrogel/sesame oil proportion and containing Span®60 and Tween®60 (batch ST1) had the highest consistency and adhesion capacity according to texture analyses. Furthermore, a genuine bigel microstructure was observed. After freeze-drying, swelling, bioadhesion and drug release tests were performed on the resulting systems. ST1 showed the longest bioadhesion time and the most controlled release, as well as a low swelling grade, becoming an interesting option for preventing HIV sexual transmission in women.
Asunto(s)
Antivirales , Hidrogeles , Tenofovir , Adhesividad , Antivirales/administración & dosificación , Antivirales/química , Línea Celular , Preparaciones de Acción Retardada/administración & dosificación , Preparaciones de Acción Retardada/química , Liberación de Fármacos , Femenino , Galactanos/administración & dosificación , Galactanos/química , Infecciones por VIH/prevención & control , Hexosas/administración & dosificación , Hexosas/química , Humanos , Hidrogeles/administración & dosificación , Hidrogeles/química , Mananos/administración & dosificación , Mananos/química , Membrana Mucosa , Gomas de Plantas/administración & dosificación , Gomas de Plantas/química , Polisorbatos/administración & dosificación , Polisorbatos/química , Aceite de Sésamo/administración & dosificación , Aceite de Sésamo/química , Tenofovir/administración & dosificación , Tenofovir/química , VaginaRESUMEN
Docetaxel has demonstrated extraordinary anticancer effects on lung cancer. However, lack of optimal bioavailability due to poor solubility and high toxicity at its therapeutic dose has hampered the clinical use of this anticancer drug. Development of nanoemulsion formulation along with biocompatible excipients aimed for pulmonary delivery is a potential strategy to deliver this poorly aqueous soluble drug with improved bioavailability and biocompatibility. In this work, screening and selection of pharmaceutically acceptable excipients at their minimal optimal concentration have been conducted. The selected nanoemulsion formulations were prepared using high-energy emulsification technique and subjected to physicochemical and aerodynamic characterizations. The formulated nanoemulsion had mean particle size and ζ-potential in the range of 90 to 110 nm and - 30 to - 40 mV respectively, indicating high colloidal stability. The pH, osmolality, and viscosity of the systems met the ideal requirement for pulmonary application. The DNE4 formulation exhibited slow drug release and excellent stability even under the influence of extreme environmental conditions. This was further confirmed by transmission electron microscopy as uniform spherical droplets in nanometer range were observed after storage at 45 ± 1 °C for 3 months indicating high thermal stability. The nebulized DNE4 exhibited desirable aerosolization properties for pulmonary delivery application and found to be more selective on human lung carcinoma cell (A549) than normal cell (MRC-5). Hence, these characteristics make the formulation a great candidate for the potential use as a carrier system for docetaxel in targeting lung cancer via pulmonary delivery.
Asunto(s)
Antineoplásicos , Docetaxel , Portadores de Fármacos , Excipientes , Nanopartículas , Tensoactivos , Antineoplásicos/administración & dosificación , Antineoplásicos/química , Línea Celular , Supervivencia Celular/efectos de los fármacos , Docetaxel/administración & dosificación , Docetaxel/química , Portadores de Fármacos/administración & dosificación , Portadores de Fármacos/química , Liberación de Fármacos , Emulsiones , Ésteres , Excipientes/administración & dosificación , Excipientes/química , Hexosas/administración & dosificación , Hexosas/química , Humanos , Nanopartículas/administración & dosificación , Nanopartículas/química , Aceite de Palma , Aceites de Plantas/administración & dosificación , Aceites de Plantas/química , Polisorbatos/administración & dosificación , Polisorbatos/química , Aceite de Cártamo/administración & dosificación , Aceite de Cártamo/química , Tensoactivos/administración & dosificación , Tensoactivos/químicaRESUMEN
Polyphenols, which are secondary plant metabolites, gain increasing research interest due to their therapeutic potential. Among them, resveratrol and curcumin are two agents showing antioxidant, anti-inflammatory, antimicrobial as well as anticarcinogenic effects. In addition to their individual therapeutic effect, increased activity was reported upon co-delivery of the two compounds. However, due to the poor water solubility of resveratrol and curcumin, their clinical application is currently limited. In this context, lipid-core nanocapsules (LNC) composed of an oily core surrounded by a polymeric shell were introduced as drug carrier systems with the potential to overcome this obstacle. Furthermore, the encapsulation of polyphenols into LNC can increase their photostability. As the attributes of the polyphenols make them excellent candidates for skin treatment, the aim of this study was to investigate the effect of co-delivery of resveratrol and curcumin by LNC upon topical application on excised human skin. In contrast to the formulation with one polyphenol, resveratrol penetrated into deeper skin layers when the co-formulation was applied. Based on vibrational spectroscopy analysis, these effects are most likely due to interactions of curcumin and the stratum corneum, facilitating the skin absorption of the co-administered resveratrol. Furthermore, the interaction of LNC with primary human skin cells was analyzed encountering a cellular uptake within 24h potentially leading to intracellular effects of the polyphenols. Thus, the simultaneous delivery of resveratrol and curcumin by LNC provides an intelligent way for immediate and sustained polyphenol delivery for skin disease treatment.
Asunto(s)
Curcumina/administración & dosificación , Portadores de Fármacos/administración & dosificación , Nanocápsulas/administración & dosificación , Absorción Cutánea , Estilbenos/administración & dosificación , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Curcumina/química , Portadores de Fármacos/química , Liberación de Fármacos , Fibroblastos/efectos de los fármacos , Extracto de Semillas de Uva/administración & dosificación , Extracto de Semillas de Uva/química , Hexosas/administración & dosificación , Hexosas/química , Humanos , Técnicas In Vitro , Nanocápsulas/química , Aceites/administración & dosificación , Aceites/química , Poliésteres/administración & dosificación , Poliésteres/química , Polifenoles/administración & dosificación , Polifenoles/química , Resveratrol , Estilbenos/químicaRESUMEN
BACKGROUND: There are two components to the clinical efficacy of pediculicides: (i) efficacy against the crawling-stages (lousicidal efficacy); and (ii) efficacy against the eggs (ovicidal efficacy). Lousicidal efficacy and ovicidal efficacy are confounded in clinical trials. Here we report on a trial that was specially designed to rank the clinical ovicidal efficacy of pediculicides. Eggs were collected, pre-treatment and post-treatment, from subjects with different types of hair, different coloured hair and hair of different length. METHOD: Subjects with at least 20 live eggs of Pediculus capitis (head lice) were randomised to one of three treatment-groups: a melaleuca oil (commonly called tea tree oil) and lavender oil pediculicide (TTO/LO); a eucalyptus oil and lemon tea tree oil pediculicide (EO/LTTO); or a "suffocation" pediculicide. Pre-treatment: 10 to 22 live eggs were taken from the head by cutting the single hair with the live egg attached, before the treatment (total of 1,062 eggs). TREATMENT: The subjects then received a single treatment of one of the three pediculicides, according to the manufacturers' instructions. Post-treatment: 10 to 41 treated live eggs were taken from the head by cutting the single hair with the egg attached (total of 1,183 eggs). Eggs were incubated for 14 days. The proportion of eggs that had hatched after 14 days in the pre-treatment group was compared with the proportion of eggs that hatched in the post-treatment group. The primary outcome measure was % ovicidal efficacy for each of the three pediculicides. RESULTS: 722 subjects were examined for the presence of eggs of head lice. 92 of these subjects were recruited and randomly assigned to: the "suffocation" pediculicide (n = 31); the melaleuca oil and lavender oil pediculicide (n = 31); and the eucalyptus oil and lemon tea tree oil pediculicide (n = 30 subjects). The group treated with eucalyptus oil and lemon tea tree oil had an ovicidal efficacy of 3.3% (SD 16%) whereas the group treated with melaleuca oil and lavender oil had an ovicidal efficacy of 44.4% (SD 23%) and the group treated with the "suffocation" pediculicide had an ovicidal efficacy of 68.3% (SD 38%). CONCLUSION: Ovicidal efficacy varied substantially among treatments, from 3.3% to 68.3%. The "suffocation" pediculicide and the melaleuca oil and lavender oil pediculicide (TTO/LO) were significantly more ovicidal than eucalyptus oil and lemon tea tree oil pediculicide (EO/LTTO) (P < 0.0001). Ranking: 1. "Suffocation" pediculicide (68.3% efficacy against eggs); 2. Melaleuca oil and lavender oil (44.4%) pediculicide; 3. Eucalyptus oil and lemon tea tree oil (3.3%) pediculicide. The "suffocation" pediculicide and TTO/LO are also highly efficacious against the crawling-stages. Thus, the "suffocation" pediculicide and TTO/LO should be recommended as first line treatments.
Asunto(s)
Insecticidas/uso terapéutico , Leptospermum , Infestaciones por Piojos/tratamiento farmacológico , Melaleuca , Aceites Volátiles/uso terapéutico , Óvulo/efectos de los fármacos , Pediculus/efectos de los fármacos , Fitoterapia , Aceites de Plantas/uso terapéutico , Dermatosis del Cuero Cabelludo/tratamiento farmacológico , Aceite de Árbol de Té/uso terapéutico , Acrilatos/administración & dosificación , Acrilatos/farmacología , Acrilatos/uso terapéutico , Animales , Alcohol Bencilo/administración & dosificación , Alcohol Bencilo/farmacología , Alcohol Bencilo/uso terapéutico , Niño , Preescolar , Método Doble Ciego , Combinación de Medicamentos , Etilaminas/administración & dosificación , Etilaminas/farmacología , Etilaminas/uso terapéutico , Eucalyptus , Aceite de Eucalipto , Hexosas/administración & dosificación , Hexosas/farmacología , Hexosas/uso terapéutico , Humanos , Insecticidas/administración & dosificación , Insecticidas/farmacología , Lavandula , Infestaciones por Piojos/parasitología , Aceite Mineral/administración & dosificación , Aceite Mineral/farmacología , Aceite Mineral/uso terapéutico , Monoterpenos/administración & dosificación , Monoterpenos/farmacología , Monoterpenos/uso terapéutico , Aceites Volátiles/administración & dosificación , Aceites Volátiles/farmacología , Óvulo/crecimiento & desarrollo , Pediculus/crecimiento & desarrollo , Aceites de Plantas/administración & dosificación , Aceites de Plantas/farmacología , Polisorbatos/administración & dosificación , Polisorbatos/farmacología , Polisorbatos/uso terapéutico , Dermatosis del Cuero Cabelludo/parasitología , Aceite de Árbol de Té/administración & dosificación , Aceite de Árbol de Té/farmacologíaRESUMEN
Vaccine therapies are increasingly being used for the treatment of various diseases, and the antigen molecules themselves are being expanded from whole microorganisms to fine molecules such as peptides. Accordingly, there is a need for new adjuvants to support these new applications. In this paper, we used pharmaceutical grade mineral oil and sorbitan monooleate to develop a new oil adjuvant formula, NH(2) , and investigated its effects on peptide vaccination at both the pre-clinical and clinical levels. The adjuvant effect of NH(2) on peptide-induced cellular immunity in mice was superior to that of Montanide ISA51VG, a commercially available incomplete Freund's adjuvant for clinical use, although no significant difference was observed between the two adjuvants on peptide-induced humoral immunity. The adjuvant effects of NH(2) were also confirmed in a Phase-I clinical trial of peptide vaccines for patients with advanced cancers. These results suggest that NH(2) is a suitable adjuvant for peptide vaccination, particularly for cancer vaccines (Phase-I clinical trial of pan-HLA type personalized peptide vaccine for advanced cancer patients, UMIN clinical trial registry number: UMIN 000000619).
Asunto(s)
Adyuvantes Inmunológicos/administración & dosificación , Antígenos de Neoplasias/inmunología , Vacunas contra el Cáncer/administración & dosificación , Hexosas/administración & dosificación , Proteínas de Unión al ARN/inmunología , Vacunación , Animales , Emulsiones , Femenino , Humanos , Inmunidad Humoral , Interferón gamma/biosíntesis , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos ICR , Linfocitos T Citotóxicos/inmunologíaRESUMEN
MSS, a comprising mixture of maesil (Prunus mume Sieb. et Zucc) concentrate, disodium succinate and Span80 (3.6:4.6 :1 ratio) showed a significant improvement of memory when daily administered (460 mg/kg day, p.o.) into the normal rats for 3 weeks. During the spatial learning of 4 days in Morris water maze test, both working memory and short-term working memory index were significantly increased when compared to untreated controls. We investigated a molecular signal transduction mechanism of MSS on the behaviors of spatial learning and memory. MSS treatment increased hippocampal mRNA levels of NR2B and TrkB without changes of NR1, NR2A, ERK1, ERK2 and CREB. However, the protein levels of pERK/ERK and pCREB/CREB were all significantly increased to 1.5+/-0.17 times. These results suggest that the improving effect of spatial memory for MSS is linked to MAPK/ERK signaling pathway that ends up in the phosphorylation of CREB through TrkB and/or NR2B of NMDA receptor.
Asunto(s)
Hexosas/farmacología , Hipocampo/efectos de los fármacos , Hipocampo/metabolismo , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Memoria/efectos de los fármacos , Extractos Vegetales/farmacología , Prunus/metabolismo , Animales , Antidepresivos/administración & dosificación , Antidepresivos/farmacología , Activación Enzimática/efectos de los fármacos , Hexosas/administración & dosificación , Hipocampo/enzimología , Masculino , Aprendizaje por Laberinto , Extractos Vegetales/administración & dosificación , Ratas , Ratas Sprague-DawleyRESUMEN
No anthelmintic sugars have yet been identified. Eight ketohexose stereoisomers (D- and L-forms of psicose, fructose, tagatose and sorbose), along with D-galactose and D-glucose, were examined for potency against L1 stage Caenorhabditis elegans fed Escherichia coli. Of the sugars, D-psicose specifically inhibited the motility, growth and reproductive maturity of the L1 stage. D-Psicose probably interferes with the nematode nutrition. The present results suggest that D-psicose, one of the rare sugars, is a potential anthelmintic.
Asunto(s)
Antinematodos/farmacología , Caenorhabditis elegans/efectos de los fármacos , Fructosa/farmacología , Animales , Antinematodos/administración & dosificación , Antinematodos/química , Fructosa/administración & dosificación , Fructosa/química , Galactosa/administración & dosificación , Galactosa/química , Galactosa/farmacología , Glucosa/administración & dosificación , Glucosa/química , Glucosa/farmacología , Hexosas/administración & dosificación , Hexosas/química , Hexosas/farmacología , Larva/efectos de los fármacos , Larva/crecimiento & desarrollo , Pruebas de Sensibilidad Parasitaria , Sorbosa/administración & dosificación , Sorbosa/química , Sorbosa/farmacología , Estereoisomerismo , Relación Estructura-ActividadRESUMEN
Tagatose, a low-calorie, full-bulk natural sugar, has just attained GRAS (Generally Recognized As Safe) status under U.S. Food and Drug Administration (FDA) regulations, thereby permitting its use as a sweetener in foods and beverages. This paper presents all current aspects of tagatose with respect to demonstrated food and beverage applications and the potential health and medical benefits of this unique substance. Summarized studies are referenced to detailed peer-reviewed papers. The safety studies followed the recommendations in the FDA "Red Book." Results were submitted to an Expert Panel for determination of GRAS status under FDA regulation. Small phase 2 clinical trials showed tagatose to be effective in treating type 2 diabetes. The results, buttressed by the references cited, support the efficacy of the various applications disclosed for tagatose. Tagatose has been found to be safe and efficacious for use as a low-calorie, full-bulk sweetener in a wide variety of foods, beverages, health foods, and dietary supplements. It fills broad, heretofore unmet needs for a low-calorie sweetener in products in which the bulk of sugar is important, such as chocolates, chewing gum, cakes, ice cream, and frosted cereals. Its synergism with high-intensity sweeteners also makes it useful in sodas. Various health and medical benefits are indicated, including the treatment of type 2 diabetes, hyperglycemia, anemia, and hemophilia and the improvement of fetal development.