Asunto(s)
Arachis/inmunología , Técnicas y Procedimientos Diagnósticos/normas , Glycine max/inmunología , Isotretinoína/uso terapéutico , Hipersensibilidad al Cacahuete/tratamiento farmacológico , Anafilaxia/inmunología , Anafilaxia/prevención & control , Antígenos de Plantas/inmunología , Arachis/efectos adversos , Bases de Datos Factuales , Fármacos Dermatológicos/administración & dosificación , Fármacos Dermatológicos/uso terapéutico , Humanos , Inmunoglobulina E/inmunología , Isotretinoína/administración & dosificación , Monitoreo Fisiológico/métodos , Hipersensibilidad a la Nuez/tratamiento farmacológico , Hipersensibilidad a la Nuez/inmunología , Hipersensibilidad a la Nuez/prevención & control , Hipersensibilidad al Cacahuete/inmunología , Aceite de Soja/metabolismo , Glycine max/efectos adversosRESUMEN
The study investigated the influence of atmospheric plasma processing on cashew nut composition as well as on its allergenicity. The cashew nuts were processed by low-pressure plasma, using glow discharge plasma (80â¯W and 50â¯kHz power supply). Anacardic acids and allergens were quantified by HPLC and immunoassay, respectively. Additionally, the overall composition was evaluated by 1H qNMR. Increases in amounts of anacardic acids (15:1, 15:2, and 15:3) and fatty acids (oleic, linoleic, palmitic and stearic) were detected after all process conditions, with 70.92% of total variance captured using 2 LVs. The total amount of anacardic acids increased from 0.7 to 1.2⯵g·mg-1 of nut. The major change was observed for anacardic acid (C15:3) with an increase from 0.2 to 0.55⯵g/mg of nut for the samples treated with a flow of 10â¯mL·min-1 and 30â¯min of processing. On the other hand, the amount of sucrose decreased, from 33 to 18â¯mg·g-1 of nut, after all processing conditions. Plasma processing of cashew nuts did not affect binding of either the rabbit anti-cashew or human cashew allergic IgE binding. Among the treatments, 10â¯min of plasma processing at flow rate of 30â¯mL·min-1 of synthetic air followed by 20â¯min at flow rate 5.8â¯mL·min-1 had the least effect on nut composition as a whole.