Enamel growth rates of anterior teeth in males and females from modern and ancient British populations.

OBJECTIVE: This study explored biological sex differences in the regional daily growth rates of human anterior enamel from modern and ancient populations in Britain. METHODS: Maxillary permanent incisors (n = 80) and canines (n = 69) from Roman, Anglo-Saxon, Medieval, and Modern day populations were analyzed using histological methods. Daily secretion rates (DSRs) were collected for inner, mid, and outer regions of cuspal and lateral enamel. Modern day samples were of known sex, archeological individuals had sex determined using standard osteological methods. Variation in DSRs between the sexes, both between and within populations, was sought using parametric and nonparametric tests. RESULTS: When all samples were pooled, there was no significant difference between males and females. Similarly no significant differences in DSRs were identified between male and females within each population. When DSRs were compared between the populations, DSRs decreased from the more ancient to the more recent populations for males, and for females. More interpopulation differences were observed in males. DISCUSSION: This study presents evidence for the relative consistency of enamel DSRs between male and female groups within each British population. Interpopulation analyses found DSRs slowed significantly between Roman and modern day populations for both sexes, with male DSRs showing the greatest variation between populations.

Middle Stone Age human teeth from Magubike rockshelter, Iringa Region, Tanzania.

In 2006, six isolated hominin teeth were excavated from Middle Stone Age (MSA) deposits at the Magubike rockshelter in southern Tanzania. They comprise two central incisors, one lateral incisor, one canine, one third premolar, and one fourth premolar. All are fully developed and come from the maxilla. None of the teeth are duplicated, so they may represent a single individual. While there is some evidence of post-depositional alteration, the morphology of these teeth clearly shares features with anatomically modern Homo sapiens. Both metric and non-metric traits are compared to those from other African and non-African dental remains. The degree of biological relatedness between eastern and southern African Stone Age hunter-gatherers has long been a subject of interest, and several characteristics of the Magubike teeth resemble those of the San of southern Africa. Another notable feature is that the three incisors are marked on the labial crown by scratches that are much coarser than microwear striations. These non-masticatory scratches on the Magubike teeth suggest that the use of the front teeth as tools included regularly repeated activities undertaken throughout the life of the individual. The exact age of these teeth is not clear as ESR and radiocarbon dates on associated snail shells give varying results, but a conservative estimate of their minimum age is 45,000 years.

Storing Tooth Segments for Optimal Esthetics.

OBJECTIVE: A fractured whole crown segment can be reattached to its remnant; crowns from extracted teeth may be used as pontics in splinting techniques. We aimed to evaluate the effect of different storage solutions on tooth segment optical properties after different durations. STUDY DESIGN: Sixty central incisor crowns were divided into 6 groups (n = 10); Group 1 was kept dry; Groups 2, 3, 4, 5, and 6 were placed in an isotonic solution, water, milk, saliva, and casein-phosphopeptide-amorphous-calcium-phosphate (CPP-ACP), respectively, for 30 min, 12 h, 1 day, 1 week, and 3 weeks. Color values were measured using a colorimeter. Data were analyzed with Kruskal-Wallis tests, Mann-Whitney U-tests, and Friedman Wilcoxon tests with Bonferroni stepwise corrections (p < 0.05). RESULTS: ΔE* values varied from 0.3 to 15.3 over the 3 week period. Group 1 demonstrated the greatest color changes over all durations; Group 6 exhibited the least. L*, a*, b*, and ΔE* values varied between time periods in all groups; the differences were significant (p < 0.01), except for L* and ΔE* values in Group 2 and a* values in Group 6 (p > 0.01). Comparing ΔE* values, Group 6 was significantly different from the other groups for all durations (p < 0.01), except Group 4. CONCLUSIONS: A CPP-ACP complex solution seems a good choice for tooth fragment storage. Milk and saliva solutions may cause perceptible color changes if tooth fragments are stored for 3 weeks before use.

Influência do tratamento de superfície da dentina radicular na resistência ao deslocamento de pino de fibra de vidro / Influence of root dentin surface treatment on bond strength of glass fiber post

Este trabalho avaliou a influência do tratamento de superfície na dentina radicular na resistência de união com pinos de fibra de vidro e cimento resinoso autoadesivo. Coletou-se 120 dentes incisivos inferiores bovinos dos quais 30 foram selecionados e tiveram suas coroas cortadas, permanecendo o remanescente radicular de 16 mm. Foi realizado o tratamento endodôntico e preparo intracanal para o pino, com profundidade de 8 mm para cimentação com cimento resinoso autoadesivo. As amostras foram divididas, randomicamente, em três grupos, segundo o tipo de tratamento de superfície, para limpeza intracanal após o preparo para cimentação: Grupo I - água destilada; Grupo II - EDTA 17% e Grupo III - clorexidina 0,12%. Os corpos de prova formados pelo conjunto dente-pino foram cortados no terço cervical, médio e apical, obtendo espécimes com 2,0 mm de espessura. Os espécimes foram levados à máquina de ensaios universal com velocidade de 0,5 mm/min para teste de resistência ao cisalhamento æpush-outÆ. A análise dos resultados foi feita com o teste ANOVA e Teste de Tukey (p<0,05). Os efeitos da água destilada, clorexidina 0,12% e EDTA 17% foram diferentes no tratamento superficial da dentina radicular, havendo diferença na resistência de união entre pinos de fibra de vidro e cimento resinoso autoadesivo nos terços cervical (p<0,013) e médio (p<0,026), sendo que o grupo da clorexidina 0,12% teve os melhores resultados. Isto permite sugerir novo protocolo de cimentação, com ação desinfetante (clorexidina), sem comprometer a resistência de união...

This study has evaluated the influence of different root canal dentin pre-treatments on the bond strength of radicular glass fiber posts. One hundred twenty bovine lower incisors were collecte. Thirty of them were selected and had their roots sectioned 16 mm from their apices. , Endodontic treatment and post space preparation to 8 mm deep were performed for cementation with self-adhesive resin cement. Samples were randomly divided into three according to surface treatments: Group I - irrigation with distilled water; Group II - irrigation with 17% EDTA; and Group III - irrigation with 0.12% chlorhexidine. Roots were then cut into 3 discs of 2 mm thick each and separated according to the region into cervical, medial and apical discs. A push-out bond strength test was performed by a universal testing machine at a crosshead speed of 0.5 mm/min. Data were analyzed with one way analysis of variance (ANOVA) and Tuckey test (p<0.05). Mean bond strength values showed significant difference between the groups in the cervical region (p<0.013) and medial region (p<0.026), that suggest a new bond protocol with disinfectant effect using 0.12% chlorhexidine without negative impact on bond strength...

The effect of the season of birth and of selected maternal factors on linear enamel thickness in modern human deciduous incisors.

OBJECTIVE: Development of human tooth enamel is a part of a foetus's development; its correctness is the outcome of genetic and maternal factors shaping its prenatal environment. Many authors reported that individuals born in different seasons experience different early developmental conditions during pregnancy. In this study, we investigated the effects of season of birth and selected maternal factors on enamel thickness of deciduous incisors. DESIGN: Dental sample comprises 60 deciduous incisors. The parents who handed over their children's teeth for research fill in questionnaires containing questions about the course of pregnancy. All teeth were sectioned in the labio-linqual plane using diamond blade (Buechler IsoMet 1000). The final specimens were observed by way of scanning electron microscopy at magnifications 80× and 320×. The thickness of total enamel (TE), prenatally (PE) and postnatally (PSE) formed enamel was measured. RESULTS: Children born in summer and in spring (whose first and second foetal life fall on autumn and winter) have the thinnest enamel. Season of birth, number of children in family, diseases and spasmolytic medicines using by mother during pregnancy explained almost 13% of the variability of TE. Regression analysis proved a significant influence of the season of birth and selected maternal factors on the PE thickness - these factors explained over 17% of its variability. Neither of analysed variables had influenced PSE. CONCLUSIONS: Our findings suggests that the thickness of enamel of deciduous incisors depends on the season of birth and some maternal factors. The differences were observed only in the prenatally formed enamel.

Color variation between matched and fabricated shades of different ceramics.

PURPOSE: The aim of this study was to evaluate and compare the total color difference (ΔE) between natural teeth and fabricated crowns from three ceramic systems with different thicknesses. MATERIALS AND METHODS: The color of ninety maxillary central incisors was measured from the middle third of the labial surface with a Vita Easyshade spectrophotometer. All-ceramic crown preparations with different thicknesses (0.8, 1.2, 1.5 mm) were done on selected teeth (n = 30). Prepared teeth were randomly divided into three equal groups to fabricate ceramic crowns from three ceramic systems, Duceram LFC (DLFC), In-Ceram SPINELL (ICS), and IPS Empress (IPSE). Colors of cemented crowns were measured and compared with their corresponding measurements before preparations. Data were statistically analyzed using two-way ANOVA at 5% significance level. RESULTS: A significant difference of ΔE was detected between natural teeth and different thicknesses of crowns constructed from the all-ceramic materials investigated. Comparing the three materials at 0.8 mm thickness revealed that the lowest ΔE was recorded for DLFC, which was significantly different from the other ceramic systems while IPSE showed the highest ΔE. At higher thicknesses there was no difference between natural tooth shade and crowns constructed from different ceramic materials. CONCLUSIONS: Reinforcement of ceramics by alumina for In-Ceram and leucite for Empress decreases color production. Level of acceptance between the different ceramic materials and thicknesses varied. DLFC showed the highest color matching at all thicknesses followed by ICS and IPSE in descending order. In general, increasing the thickness of fabricated crowns enhances color match.

Clinical evaluation of the effectiveness of different bleaching therapies in vital teeth.

The aims of this in vivo study were to compare the effectiveness and color stability of at-home and in-office bleaching techniques and to evaluate whether the use of light sources can alter bleaching results. According to preestablished criteria, 40 patients were selected and randomly divided into four groups according to bleaching treatment: (1) at-home bleaching with 10% carbamide peroxide, (2) in-office bleaching with 35% hydrogen peroxide (HP) without a light source, (3) in-office bleaching with 35% HP with quartz-tungsten-halogen light, and (4) in-office bleaching with 35% HP with a light-emitting diode/laser. Tooth shade was evaluated using the VITA Classical Shade Guide before bleaching as well as after the first and third weeks of bleaching. Tooth shade was evaluated again using the same guide 1 and 6 months after the completion of treatment. The shade guide was arranged to yield scores that were used for statistical comparison. Statistical analysis using the Kruskal-Wallis test showed no significant differences among the groups for any time point (P > .01). There was no color rebound in any of the groups. The bleaching techniques tested were equally effective. Light sources are unnecessary to bleach teeth.

Morphological features of the maxillary incisors roots and relationship with neighbouring anatomical structures: possible implications in endodontic surgery.

The purpose of this study was to investigate the relationship between the root apex of the upper incisors and neighbouring anatomical structures as well as the morphology of the root-end foramen after apicoectomy. Fifty-seven patients requiring endodontic surgical treatment for a maxillary anterior root were enrolled. A preoperative diagnostic computed tomography (CT) scan was analysed to determine: the distance between the anterior wall of the nasopalatine duct and the central (CI-ND) incisor root 4mm from the apex; and the distance between the floor of the nasal cavity and the tip of either the central (CI-NF) or the lateral (LI-NF) incisor root. After apicoectomy, root-end foramen endoscopic pictures were taken in order to characterize their morphology. Fifty-nine central and 26 lateral incisors were evaluated. The average CI-ND was 4.71 ± 1.26 (SD) mm. The average CI-NF was 10.62 ± 2.25 mm. The average LI-NF was 13.05 ± 2.43 mm. The foramen shape after apicoectomy was ovoid to circular in about 90% of cases in both central and lateral incisors. A sound knowledge of the anatomical relationships at the surgical site is essential for the clinician to perform a safe endodontic surgical procedure.

Effect of physical and physicochemical surface treatment methods on the tensile strength of CAD/CAM-fabricated zirconia posts and cores luted to root canals.

This study investigated the effect of surface treatments on the retentive strength of zirconia posts and cores to root canals. Maxillary central incisors (n = 40) were treated endodontically, obturated, and post spaces were prepared. Zirconia posts and cores (n = 40) were obtained and assigned randomly to four groups (n = 10 per group; control, sandblasting, tribochemical silica coating, and tribochemical silica coating + silanization). Posts were cemented adhesively, and tensile force was applied. All treatment methods increased the tensile strength of zirconia posts and cores compared to the control group (P = .034). No significant difference was found between treatment methods. Failure types varied depending on the surface treatment method.

Pilot in vivo image spectro-photometric evaluation of optical properties of pure enamel and enamel-dentin complex.

OBJECTIVES: The aim of this in vivo study is to investigate the L*a*b*and the opacity (CR) of front teeth by means of an image spectrophotometer and to evaluate the eventual influence of the background color on the results. The second aim is to investigate if there is a relationship between tea, coffee, red wine drinking habits or smoking habits of the test subjects and tooth color. METHODS: A novel image based spectro-photometric approach was developed and applied on a Swiss Army recruits group quantifying L*a*b* of pure enamel as well as of enamel-dentin complex against black and white background together with CR. RESULTS: When 2mm thick pure enamel was considered, the values obtained were (mean (SD)) L*(76.3 (3.4)), a*(3.4 (1.2)) and b*(17.2 (2.45)) against white background and L*(63.5 (4.2)), a*(0.8 (1.3)) and b*(10.7 (2.7)) against black background. The opacity (CR) of 2mm thick pure enamel was (64.4 (0.1)). When 3mm thick enamel-dentin complex was considered, the values obtained were L*(79.0 (2.6)), a*(3.9 (1.3)) and b*(20.4 (3.0)) against a white background and L*(74.9 (3.0)), a*(1.8 (1.2)) and b*(16.7 (3.1)) against a black background. The opacity (CR) of 3mm thick enamel-dentin complex was (87.4 (0.1)). SIGNIFICANCE: The application of this method on a larger group of subjects of different ages may serve as a database for a more exact characterization of optical properties of natural enamel and dentin.

Nonlinear visco-elastic finite element analysis of porcelain veneers: a submodelling approach to strain and stress distributions in adhesive and resin cement.

PURPOSE: To assess under load the biomechanical behavior of the cementing system of feldspathic vs alumina porcelain veneers. MATERIALS AND METHODS: A 3D model of a maxillary central incisor, the periodontal ligament (PDL) and the alveolar bone was generated. Incisors restored with alumina and feldspathic porcelain veneers were compared to a natural sound tooth. Enamel, cementum, cancellous and cortical bone were considered isotropic elastic materials; conversely, dentin was designated as orthotropic. The nonlinear visco-elatic behavior of the PDL was considered. The adhesive layers were modelled using spring elements. A 50-N load at a 60-degree angle to the tooth's longitudinal axis was applied and validated. Stress concentration in the interfacial volumes of the main models was identified and submodelled in a new environment. RESULTS: Regarding tooth structure, strain concentrations were observed in the root dentin below the CEJ. As to the cement layer, tensile stresses concentrated in the palatal margin of the adhesive complex. CONCLUSION: Despite the effects on tooth deformation, the rigidity of the veneer did not affect the stress distributions in the cement layer or in the adhesive layers. In both cases, the palatal and cervical margins seemed to be the most stressed areas.

Freqüência clínica dos biótipos periodontais considerando o formato geométrico dos incisivos centrais superiores / Clinical frequency of periodontal biotypes among different geometric forms of maxillary incisors

O objetivo deste estudo clínico foi avaliar a freqüência dos biótipos periodontais, considerando os diferentes formatos geométricos dos incisivos centrais superiores, através de parâmetros anatômicos dentários e gengivais. Quarenta voluntários foram agrupados de acordo com a forma dos incisivos centrais superiores (ICS, n = 10): retangular (grupo 1), triangular (grupo 2), quadrado (grupo 3) ou oval (grupo 4). Para cada voluntário, foi determinado o biótipo periodontal através de consenso entre 3 examinadores calibrados e foram medidos: o comprimento (CC) e a largura (LC) da coroa; a razão entre a largura e o comprimento da coroa (PLC); a altura do ponto de contato entre os ICS (APC); a distância entre os zênites gengivais dos ICS (ZG1); a distância entre os zênites gengivais do incisivo central e do incisivo lateral superiores (ZG2); a altura da papila entre os ICS (AP1); e a altura da papila entre o incisivo central e o incisivo lateral superiores (AP2). Os dentes quadrados obtiveram os menores valores de comprimento coronário (9,36 ± 0,97, p = 0,0012) e a maior proporção entre a largura e o comprimento da coroa (0,93 ± 0,06, p = 0,0001), quando comparados aos dos demais grupos. Os dentes triangulares apresentaram significativamente a maior distância (8,44 ± 0,89, p = 0,0438) considerando o parâmetro ZG2. O biótipo periodontal fino/festonado foi predominante no total de voluntários desse estudo (60%), e mais freqüente em indivíduos com dentes retangulares. Voluntários com dentes quadrados apresentaram maior freqüência de periodonto plano e espesso (60%), embora o teste exato de Fisher não tenha encontrado diferenças estatisticamente significantes entre os grupos.

The aim of this clinical study was to evaluate the frequency of periodontal phenotypes among different geometric forms of maxillary central incisors. Some clinical parameters such as tooth and papillae dimension, localization of contact points and distances between gingival vertices were also evaluated. Forty patients were selected and divided into 4 groups (n=10) according to geometric form of maxillary central incisors: rectangular (group 1), triangular (group 2), squared (group 3) or ovoid (group 4). Squared teeth showed the lowest coronal length values (9.36±0.97; p=0.0012 and the highest coronal width/length ratio (0.93±0.06; p=0.0001), when compared to the other groups. Thin and scalloped gingival phenotype was predominant in this clinical study (60%), and was frequent in groups 1, 2 and 4. In contrast, squared teeth (group 3) presented predominantly thick and plan gingiva. Nevertheless, Fisher’s exact test did not found any statistical difference among groups.

Non-masticatory uses of anterior teeth of Sima de los Huesos individuals (Sierra de Atapuerca, Spain).

In this study we examine the labial and occlusal surfaces of incisors and canines of hominins recovered from the Sima de los Huesos (SH), middle Pleistocene site, in order to establish the possible extra-masticatory use of anterior teeth. We have compared the microwear of these fossils with microwear from the anterior teeth of Australian Aborigines, a population characterized by ethnographic evidence of the use of their teeth as a third hand. These two samples of teeth were microscopically analyzed using Scanning Electron Microscopy (SEM). Our results support the "cultural" origin of microwear observed on fossil teeth: we conclude that the SH hominins used their anterior teeth as a "third hand" for para- or extra-masticatory activities.

Involvement of the klotho protein in dentin formation and mineralization.

Klotho-deficient mice exhibit multiple pathological conditions resembling human aging. Our previous study showed alterations in the distribution of osteocytes and in the bone matrix synthesis in klotho-deficient mice. Although the bone and tooth share morphological features such as mineralization processes and components of the extracellular matrix, little information is available on how klotho deletion influences tooth formation. The present study aimed to elucidate the altered histology of incisors of klotho-deficient mice-comparing the findings with those from their wild-type littermates, by using immunohistochemistry for alkaline phosphatase (ALP), osteopontin, and dentin matrix protein-1 (DMP-1), terminal deoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick end-labeling (TUNEL) detection for apoptosis, and electron probe microanalyzer (EPMA) analysis on calcium (Ca), phosphate (P), and magnesium (Mg). Klotho-deficient incisors exhibited disturbed layers of odontoblasts, predentin, and dentin, resulting in an obscure dentin-predentinal border at the labial region. Several odontoblast-like cells without ALP activity were embedded in the labial dentin matrix, and immunopositivity for DMP-1 and osteopontin was discernible in the matrix surrounding these embedded odontoblast-like cells. TUNEL detection demonstrated an apoptotic reaction in the embedded odontoblast-like cells and pulpal cells in the klotho-deficient mice. EPMA revealed lower concentrations of Ca, P, and Mg in the klotho-deficient dentin, except for the dentin around abnormal odontoblast-like cells. These findings suggest the involvement of the klotho gene in dentinogenesis and its mineralization.

Prevention of brick tea fluorosis in rats with low-fluoride brick tea on laboratory observation.

To test whether low-fluoride brick tea can prevent the occurrence of fluorosis, rats had access only to a specially prepared low-fluoride brick tea for 1 year. The daily fluoride intake, fluoride metabolism, tissue distribution and development of tooth fluorosis were observed at 4-monthly intervals, at the end of months 4, 8 and 12, respectively. Rats drinking ordinary brick tea (F- 503.5 mg/kg) served as control. The daily intake of fluoride in the ordinary brick tea group was 0.3 mg, and this group developed dental fluorosis characterized as brown and white horizontal marks at the end of month 8, and white chalky dental fluorosis developed at the end of month 12. The total incidence was 75%. In contrast, the daily fluoride intake of the low-fluoride brick tea (F- 210 mg/kg) group was 0.19 mg, and this group did not develop any signs of dental fluorosis. Fluoride distribution was mainly retained in the bone tissue, and about half of the absorbed fluoride was excreted via urine and feces. The results suggest that this low-fluoride brick tea did not induce fluorosis in rats and can be used as an effective control measure for humans.

Dental variation among four prehispanic Mexican populations.

In this paper, the dental morphology of prehispanic Meso-american populations is described, compared, and examined within the context of New World dental variation. Twenty-eight morphological dental traits were studied and compared in four samples of prehispanic Mexican populations. After eliminating intra- and interobserver error, the dental morphological characteristics observed show evidence of heterogeneity among the populations. In particular, the oldest population, Tlatilco (1300-800 BC), was significantly different from the other three groups, Cuicuilco (800-100 BC), Monte Albán (500 BC-700 AD) and Cholula (550-750 AD). When the four samples were compared to other Mongoloid populations, either univariately or multivariately, it was observed that the Mexican groups did not follow a strict Sinodont (characteristic of Northeast Asia)/Sundadont (characteristic of Southeast Asia) classification (Turner [1979] Am. J. Phys. Anthropol. 51:619-636). From the traits examined, 27% presented frequencies consistent with Sinodont variation, while 73% of the traits showed similar incidence to Southeast Asian groups. Multivariately, the Mexican populations were found to fit an overall Sundadont classification. These results indicate that there is more dental morphological variation among American Indian populations than previously shown.

Deciduous dental morphology of the prehistoric Jomon people of Japan: comparison of nonmetric characters.

Morphological variations of the deciduous dentition are as useful as those of the permanent dentition for determining the biological affinities of human populations. This paper provides material on morphological variations of deciduous teeth of the prehistoric Japanese population from the Late and the Latest Jomon Period (ca. 2000-ca. 300 B.C.). The expression of nonmetric traits of the deciduous teeth in the Jomon sample shows a closer affinity with modern Japanese and Native American samples than with American White, Asiatic Indian, and African samples. However, the frequency of shoveling in deciduous upper incisors in the Jomon sample is lower than those in modern Japanese and Native American samples. The Jomon sample also expresses a much higher frequency of cusp 6 in deciduous lower second molars than seen in modern Japanese, Ainu, and Native American samples. The frequency in the Jomon sample is equal to that in the Australian Aboriginal sample, which shows cusp 6 most frequently among the samples compared. A somewhat low incidence of incisor shoveling in the Jomon sample was also reported in the permanent dentition (Turner [1976] Science 193:911-913, [1979] Am. J. Phys. Anthropol. 51:619-635, [1987] Am. J. Phys. Anthropol. 73:305-321, [1990] Am. J. Phys. Anthropol. 82:295-317; T. Hanihara [1992] Am. J. Phys. Anthropol. 88:163-182, 88:183-196). However, the frequency of cusp 6 in the Jomon sample shows no significant difference from those of Northeast Asian or Native American samples in the permanent dentition (Turner [1987] Am. J. Phys. Anthropol. 73:305-321; T. Hanihara [1992] Am. J. Phys. Anthropol. 88:163-182, 88:183-196). Evidently, some nonmetric traits express an inter-group difference only in the deciduous dentition.

More lingual surface attrition of the maxillary anterior teeth in American Indians: prehistoric Panamanians.

Lingual surface attrition of the maxillary anterior teeth (LSA-MAT) has been found in additional prehistoric Latin American Indian skeletons. LSAMAT was first observed in crania from an Archaic Brazilian site. This second finding occurs in teeth from Venado Beach, a late prehistoric site in Panama. LSAMAT is also present in some fragmentary specimens from the Archaic Cerro Mangote site in Panama. LSAMAT at Venado Beach is present in 57% of 28 adult crania. As in the Brazilian study, LSAMAT is associated with a high caries rate (82% of 50 adults; 11.7% of 852 permanent teeth). As first suggested, eating and processing of some type of abrasive carbohydrate food, such as manioc, is the possible cause of LSAMAT. However, other possible causes relating to habitation on or near marine coasts cannot be totally ruled out.