Growing up in Ancient Sardinia: Infant-toddler dietary changes revealed by the novel use of hydrogen isotopes (δ2H).

Detailed information about the lives and deaths of children in antiquity is often in short supply. Childhood dietary histories are, however, recorded and maintained in the teeth of both juveniles and adults. Primary tooth dentinal collagen does not turn over, preserving a sequential record of dietary changes. The use of nitrogen (δ15N) and carbon (δ13C) isotope values of incrementally sampled dentin are used in the study of breastfeeding practices but evidence for the addition of weaning foods, both in terms of mode and, particularly, duration, has remained analytically inaccessible to date. Here, we demonstrate how the novel use hydrogen isotope (δ2H) values of sequentially micro-sampled dentin collagen, measured from individuals excavated from a Punic cemetery, in Sardinia, Italy, can serve as a proxy for weaning food type and duration in ancient childhood diet. The weaning rate and age, based on the decline in δ15N and δ13C values of permanent first molars and the concomitant increase in δ2H, appears to be broadly similar among six individuals. Hydrogen isotopes vary systematically from a low value soon after birth, rising through early childhood. The early post-birth values can be explained by the influence of 2H-depleted lipids from mother's breastmilk and the later δ2H rise is consistent with, among other things, a substantial portion of boiled foodstuffs, such as the higher δ2H values observed in porridge. Overall δ2H in dentin shows great promise to elucidate infant and childhood feeding practices, and especially the introduction of supplementary foods during the weaning process.

Multi-proxy stable isotope analyses of dentine microsections reveal diachronic changes in life history adaptations, mobility, and tuberculosis-induced wasting in prehistoric Liguria (Finale Ligure, Italy, northwestern Mediterranean).

OBJECTIVE: To reconstruct breastfeeding and weaning practices, metabolic stress including tuberculosis-induced wasting, and residential mobility of children in Neolithic and Metal Ages to infer their local ecologies. MATERIALS: Seven permanent teeth from individuals dated to the Neolithic, Copper, Bronze, and Iron Ages buried in nearby caves in western Liguria, Italy. METHODS: Carbon, nitrogen, and sulfur stable isotope analyses on dentine microsections. Tooth maturation was used to calculate age at death. RESULTS: Two Neolithic children present longer pattern of weaning and appear to have been weaned using animal protein in contrast to the earlier weaning of Metal Ages children, which were probably weaned with vegetable resources. Sulfur isotopes suggest local origin of Neolithic and Cooper Age children, and non-local origins for Bronze and Iron Age children. Intense catabolism in the last two years is apparent in the adolescent with tuberculosis. CONCLUSIONS: Shortening in weaning patterns during the Metal Ages are likely driven by the intensification of agricultural practices and cultivation of new crops during Bronze and Iron Ages. Neolithic food choices and delayed weaning patterns may represent one of the strategies to maximize growth and immune potential in a local economy/ecology with high-infectious load. Tuberculosis was a chronic and long-lasting disease. SIGNIFICANCE: The first combined carbon, nitrogen, and sulfur analysis on prehistoric dentine microsections revealing changing human life history adaptations within the same region. LIMITATIONS: Small sample size. SUGGESTIONS FOR FURTHER RESEARCH: Increase the sulfur isotope dataset, use new EA-IRMS equipment, and provide data on amino acid to better define weaning food composition.

Let's talk about stress, baby! Infant-feeding practices and stress in the ancient Atacama desert, Northern Chile.

AIMS AND OBJECTIVES: The transition to an agricultural economy is often presumed to involve an increase in female fertility related to changes in weaning practice. In particular, the availability of staple crops as complementary foods is hypothesized to allow earlier weaning in agricultural populations. In this study, our primary aim is to explore whether this model fits the agricultural transition in the Atacama Desert using incremental isotopic analysis. A secondary aim of this study is to identify isotopic patterns relating to weaning, and assess how these may be differentiated from those relating to early life stress. MATERIALS AND METHODS: We use incremental isotopic analysis of dentine to examine changes in δ15 N and δ13 C values from infancy and childhood in sites of the Arica region (n = 30). We compare individuals from pre-agricultural and agricultural phases to establish isotopic patterns and relate these patterns to maternal diet, weaning trajectory and physiological stress. RESULTS: We find that there is no evidence for systematic temporal or geographic variation in incremental isotopic results. Instead, results from all time periods are highly variable, with weaning completed between 1.5 and 3.5 years. Characteristics of the incremental profiles indicate that both in utero and postnatal stress were a common part of the infant experience in the Atacama. DISCUSSION: In the Atacama Desert it appears that the arrival of agricultural crops did not result in uniform shifts in weaning behavior. Instead, infant and child diet seems to have been dictated by the broad-spectrum diets of the mothers, perhaps as a way of mitigating the stresses of the harsh desert environment.

Breastfeeding, weaning, and dietary practices during the Western Zhou Dynasty (1122-771 BC) at Boyangcheng, Anhui Province, China.

OBJECTIVES: Here we investigate breastfeeding and weaning practices and adult dietary habits at the Western Zhou Dynasty (1122-771 BC) site of Boyangcheng () located in Anhui Province, China. In addition, we utilize the differences in bone collagen turnover rates between rib and long bones from the same individual to examine past life histories, such as changes in diet or residence. MATERIALS AND METHODS: Bone collagen from both the rib and long bones (either femora or humeri) of 42 individuals was measured for stable isotope ratios of carbon (δ13 C) and nitrogen (δ15 N). In addition, δ13 C and δ15 N values are reported for 35 animals (dogs, cows, horses, pigs, and deer). RESULTS: The human δ13 C values range from -20.7‰ to -12.0‰ with a mean value of -18.8 ± 1.6‰. The human δ15 N values range from 9.1‰ to 13.4‰ with a mean value of 10.9 ± 1.0‰. The animals display a wide range of δ13 C (-21.5‰ to -8.2‰; -15.8 ± 4.5‰) and δ15 N values (4.0‰ to 9.5‰; 6.5 ± 1.8‰). CONCLUSIONS: The adult δ13 C and δ15 N results indicate that mixed C3 (rice) and C4 (millet) terrestrial diets with varying levels of animal protein (mostly pigs and deer) were consumed. The elevated subadult δ15 N results return to adult levels by approximately 3-4 years of age, indicating that the weaning process was completed during this period. Individuals between 2 and 10 years old, with lower δ13 C and δ15 N results than the adult mean, possibly consumed more plant-based diets, and this is consistent with Chinese medical teachings ∼1500 years later during the Tang Dynasty (AD 618-907). The isotopic offsets between the ribs and long bones revealed that five adults experienced dramatic dietary shifts in their later lives, switching from predominately C3 /C4 to C3 diets. This research provides the first isotopic information about ancient Chinese breastfeeding and weaning practices and establishes a foundation for future studies to examine diachronic trends.

Infant feeding practices and childhood diet at Apollonia Pontica: Isotopic and dental evidence.

OBJECTIVE: This study analyzes deciduous dental pathology and stable isotopes to investigate the relationship between diet, feeding practices, and oral health in a subadult skeletal sample from the Greek colonial site of Apollonia Pontica, Bulgaria (mid-5th to mid-3rd Centuries BC). METHODS: Stable carbon and nitrogen isotope analysis was conducted on 74 bone collagen samples, and the deciduous dentitions of 85 individuals aged 8.5 months to 11 years were examined for evidence of caries, calculus, antemortem tooth loss, abscesses, and occlusal tooth wear. RESULTS: δ(13) C and δ(15) N values of the collagen samples indicate that weaning began between the ages of 6 months and 1 year, and was complete for most individuals by the age of 3. The isotopic data are consistent with a mixed diet of primarily terrestrial C3 resources. The dental pathology data indicate that complementary foods provided to young children had an impact on their oral health early on. Four outliers exhibited elevated δ(15) N values compared with the adult female range and lower levels of tooth wear than other members of their age groups. Possible explanations include prolonged breastfeeding, the consumption of diets elevated in (15) N, and physiological/nutritional stress. CONCLUSIONS: The deciduous dental data correlate well with the isotopic data and are consistent with later textual sources regarding the timing and duration of weaning, and the composition of complementary foods. The results of this research demonstrate the value of combining isotopic and dental evidence to investigate the dietary practices of infants and young children and the impact of these practices on oral health.

Lethal Lullabies: A History of Opium Use in Infants.

Poppy extract accompanied the human infant for more than 3 millenia. Motives for its use included excessive crying, suspected pain, and diarrhea. In antiquity, infantile sleeplessness was regarded as a disease. When treatment with opium was recommended by Galen, Rhazes, and Avicenna, baby sedation made its way into early medical treatises and pediatric instructions. Dabbing maternal nipples with bitter substances and drugging the infant with opium were used to hasten weaning. A freerider of gum lancing, opiates joined the treatment of difficult teething in the 17th century. Foundling hospitals and wet-nurses used them extensively. With industrialization, private use was rampant among the working class. In German-speaking countries, poppy extracts were administered in soups and pacifiers. In English-speaking countries, proprietary drugs containing opium were marketed under names such as soothers, nostrums, anodynes, cordials, preservatives, and specifics and sold at the doorstep or in grocery stores. Opium's toxicity for infants was common knowledge; thousands of cases of lethal intoxication had been reported from antiquity. What is remarkable is that the willingness to use it in infants persisted and that physicians continued to prescribe it for babies. Unregulated trade, and even that protected by governments, led to greatly increased private use of opiates during the 19th century. Intoxication became a significant factor in infant mortality. As late as 1912, the International Hague Convention forced governments to implement legislation that effectively curtailed access to opium and broke the dangerous habit of sedating infants.

Pioneros catalanes en la maternización de la leche de vaca / Catalan pioneers in humanizing cow´s milk

Se reúnen datos de carácter histórico relacionados con los inicios de la introducción y desarrollo de leches maternizadas en nuestro país, que tuvieron lugar durante los años de transición del siglo XIX al XX. En este proceso, destacan de forma muy especial las aportaciones de Luis Soldevila Casas y de los médicos Pau Ferrer Piera i Salvador Soler Farnés. Los productos diseñados y registrados por estos personajes estaban inspirados, básicamente, en los métodos denominados entonces laboratory milk (mezclas) y top milk (fraccionamiento). La vida comercial de estos productos fue muy corta o, incluso, inexistente (AU)

Historic data on the introduction and development of humanized milk that took place in our country during the transition from 19th to 20th century has been compiled. In this process, the contributions of Luis Soldevila Casas and the doctors Pau Ferrer Piera and Salvador Soler Farnés are highly remarkable. The products designed and registered by them were mainly inspired in the processes then known as «Laboratory milk» (blends) and «Top milk» (fractionation). But the shelf life of these products was very short, or they never existed (AU)

Weaning at Anglo-Saxon Raunds: Implications for changing breastfeeding practice in Britain over two millennia.

This study investigated stable-isotope ratio evidence of weaning for the late Anglo-Saxon population of Raunds Furnells, Northamptonshire, UK. δ(15)N and δ(13)C values in rib collagen were obtained for individuals of different ages to assess the weaning age of infants within the population. A peak in δ(15) N values at about 2-year-old, followed by a decline in δ(15) N values until age three, indicates a change in diet at that age. This change in nitrogen isotope ratios corresponds with the mortality profile from the site, as well as with archaeological and documentary evidence on attitudes towards juveniles in the Anglo-Saxon period. The pattern of δ(13) C values was less clear. Comparison of the predicted age of weaning to published data from sites dating from the Iron Age to the 19th century in Britain reveals a pattern of changing weaning practices over time, with increasingly earlier commencement and shorter periods of complementary feeding in more recent periods. Such a change has implications for the interpretation of socioeconomic changes during this period of British history, since earlier weaning is associated with decreased birth spacing, and could thus have contributed to population growth.

Barium distributions in teeth reveal early-life dietary transitions in primates.

Early-life dietary transitions reflect fundamental aspects of primate evolution and are important determinants of health in contemporary human populations. Weaning is critical to developmental and reproductive rates; early weaning can have detrimental health effects but enables shorter inter-birth intervals, which influences population growth. Uncovering early-life dietary history in fossils is hampered by the absence of prospectively validated biomarkers that are not modified during fossilization. Here we show that large dietary shifts in early life manifest as compositional variations in dental tissues. Teeth from human children and captive macaques, with prospectively recorded diet histories, demonstrate that barium (Ba) distributions accurately reflect dietary transitions from the introduction of mother's milk through the weaning process. We also document dietary transitions in a Middle Palaeolithic juvenile Neanderthal, which shows a pattern of exclusive breastfeeding for seven months, followed by seven months of supplementation. After this point, Ba levels in enamel returned to baseline prenatal levels, indicating an abrupt cessation of breastfeeding at 1.2 years of age. Integration of Ba spatial distributions and histological mapping of tooth formation enables novel studies of the evolution of human life history, dietary ontogeny in wild primates, and human health investigations through accurate reconstructions of breastfeeding history.

Conformity in diversity? Isotopic investigations of infant feeding practices in two iron age populations from Southern Öland, Sweden.

This article presents the results of a study of infant diet at two Iron Age sites on the island of Öland, Sweden. The cemetery at Bjärby contained a large number of subadults who had survived the earliest years of life, whereas most individuals at Triberga had died by 6 months of age. To investigate whether differences in infant feeding could explain the different mortality rates, the carbon, nitrogen, and sulfur stable isotope ratios of bone and tooth dentin collagen from the two sites were analyzed. Twenty-two samples from Triberga and 102 from Bjärby yielded data that could be included in the carbon and nitrogen analysis. Twelve samples from Triberga and 42 from Bjärby were included in the sulfur analysis. The results for carbon (δ(13) C: Triberga X = -18.8, s.d. = 1.1; Bjärby X = -19.8, s.d. = 0.4), nitrogen (δ(15) N: Triberga X = 12.9, s.d. = 1.5; Bjärby X = 13.4, s.d. = 1.4), and sulfur (δ(34) S: Triberga X = 8.1, s.d. = 1.1; Bjärby X = 5.8, s.d. = 1.3) suggest that diet was broadly similar at both sites and based on terrestrial resources. At Bjärby, females and high-status individuals consumed higher-trophic level protein than other males from early childhood onward. There was some indication that the contribution of marine resources to the diet may also have differed between the sexes at Triberga. No consistent differences in breast milk intake were observed between the two sites, but there was substantial variation at each. This variation may reflect an influence of gender and social status on infant feeding decisions.

Cultural practices and medical beliefs in pre-revolutionary Russia compared to modern textbook advice: did Russian women breastfeed the "wrong" way?

BACKGROUND AND AIMS: Historical accounts of infant feeding practices can inform our understanding of current-day practices and the ways in which cultural traditions are incorporated into infant care. Pre-revolutionary Russian feeding practices have not previously been summarized, to our knowledge. The purpose of this study is to collect information about pre-revolutionary feeding practices. We may then be able to better understand the motivation for suboptimal practices and tailor feeding messages to the specific population. METHODS: Materials were collected from libraries and from the Internet regarding medical, demographic, and ethnographic literature of the 19(th) century and early 21(st) century, primarily in Russian. RESULTS: Breastfeeding was pervasive in pre-revolutionary Russia, but suboptimal patterns such as withholding colostrum and early introduction of other foods and liquids were common. Breast problems were treated with folk remedies and comfort measures, some of which are similar to modern-day treatments. Around 1906, child rearing and infant feeding recommendations were subsumed by male physicians espousing the "scientific approach." CONCLUSIONS: Many of these medical recommendations were detrimental to the previously successful breastfeeding practices that, despite barriers, had allowed Russian women to continue breastfeeding for 2 years or longer.

[The traditional obstetrics, puerperal rites and child nursing among Vainakhs].

Already in the beginning of XX century the traditional healing among Vainakhs consisted ofsynthesis of rational and religious magic methods, especially in obstetrics and healing of children. Some of these methods survived in time and even nowadays keep their medical hygienic significance. Nevertheless, a lot in traditional healing of Vainakhs, the rite side in particular has today only a historical value.