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1.
Biochem J ; 478(13): 2481-2497, 2021 07 16.
Artículo en Inglés | MEDLINE | ID: mdl-34198328

RESUMEN

The COVID-19 pandemic has presented itself as one of the most critical public health challenges of the century, with SARS-CoV-2 being the third member of the Coronaviridae family to cause a fatal disease in humans. There is currently only one antiviral compound, remdesivir, that can be used for the treatment of COVID-19. To identify additional potential therapeutics, we investigated the enzymatic proteins encoded in the SARS-CoV-2 genome. In this study, we focussed on the viral RNA cap methyltransferases, which play key roles in enabling viral protein translation and facilitating viral escape from the immune system. We expressed and purified both the guanine-N7 methyltransferase nsp14, and the nsp16 2'-O-methyltransferase with its activating cofactor, nsp10. We performed an in vitro high-throughput screen for inhibitors of nsp14 using a custom compound library of over 5000 pharmaceutical compounds that have previously been characterised in either clinical or basic research. We identified four compounds as potential inhibitors of nsp14, all of which also showed antiviral capacity in a cell-based model of SARS-CoV-2 infection. Three of the four compounds also exhibited synergistic effects on viral replication with remdesivir.


Asunto(s)
Antivirales/farmacología , Evaluación Preclínica de Medicamentos , Exorribonucleasas/antagonistas & inhibidores , Metiltransferasas/antagonistas & inhibidores , Caperuzas de ARN/metabolismo , SARS-CoV-2/enzimología , Bibliotecas de Moléculas Pequeñas/farmacología , Proteínas no Estructurales Virales/antagonistas & inhibidores , Adenosina Monofosfato/análogos & derivados , Adenosina Monofosfato/farmacología , Alanina/análogos & derivados , Alanina/farmacología , Animales , Antivirales/química , Clorobencenos/farmacología , Chlorocebus aethiops , Pruebas de Enzimas , Exorribonucleasas/genética , Exorribonucleasas/aislamiento & purificación , Exorribonucleasas/metabolismo , Transferencia Resonante de Energía de Fluorescencia , Ensayos Analíticos de Alto Rendimiento , Indazoles/farmacología , Indenos/farmacología , Indoles/farmacología , Metiltransferasas/genética , Metiltransferasas/aislamiento & purificación , Metiltransferasas/metabolismo , Nitrilos/farmacología , Fenotiazinas/farmacología , Purinas/farmacología , Reproducibilidad de los Resultados , SARS-CoV-2/efectos de los fármacos , Bibliotecas de Moléculas Pequeñas/química , Especificidad por Sustrato , Trifluperidol/farmacología , Células Vero , Proteínas no Estructurales Virales/genética , Proteínas no Estructurales Virales/aislamiento & purificación , Proteínas no Estructurales Virales/metabolismo , Proteínas Reguladoras y Accesorias Virales/genética , Proteínas Reguladoras y Accesorias Virales/aislamiento & purificación , Proteínas Reguladoras y Accesorias Virales/metabolismo
2.
J Biol Chem ; 295(6): 1598-1612, 2020 02 07.
Artículo en Inglés | MEDLINE | ID: mdl-31914404

RESUMEN

Benzylisoquinoline alkaloids (BIAs) are a major class of plant metabolites with many pharmacological benefits. Sacred lotus (Nelumbo nucifera) is an ancient aquatic plant of medicinal value because of antiviral and immunomodulatory activities linked to its constituent BIAs. Although more than 30 BIAs belonging to the 1-benzylisoquinoline, aporphine, and bisbenzylisoquinoline structural subclasses and displaying a predominant R-enantiomeric conformation have been isolated from N. nucifera, its BIA biosynthetic genes and enzymes remain unknown. Herein, we report the isolation and biochemical characterization of two O-methyltransferases (OMTs) involved in BIA biosynthesis in sacred lotus. Five homologous genes, designated NnOMT1-5 and encoding polypeptides sharing >40% amino acid sequence identity, were expressed in Escherichia coli Functional characterization of the purified recombinant proteins revealed that NnOMT1 is a regiospecific 1-benzylisoquinoline 6-O-methyltransferase (6OMT) accepting both R- and S-substrates, whereas NnOMT5 is mainly a 7-O-methyltransferase (7OMT), with relatively minor 6OMT activity and a strong stereospecific preference for S-enantiomers. Available aporphines were not accepted as substrates by either enzyme, suggesting that O-methylation precedes BIA formation from 1-benzylisoquinoline intermediates. Km values for NnOMT1 and NnOMT5 were 20 and 13 µm for (R,S)-norcoclaurine and (S)-N-methylcoclaurine, respectively, similar to those for OMTs from other BIA-producing plants. Organ-based correlations of alkaloid content, OMT activity in crude extracts, and OMT gene expression supported physiological roles for NnOMT1 and NnOMT5 in BIA metabolism, occurring primarily in young leaves and embryos of sacred lotus. In summary, our work identifies two OMTs involved in BIA metabolism in the medicinal plant N. nucifera.


Asunto(s)
Bencilisoquinolinas/metabolismo , Metiltransferasas/metabolismo , Nelumbo/enzimología , Proteínas de Plantas/metabolismo , Alcaloides/metabolismo , Secuencia de Aminoácidos , Vías Biosintéticas , Metiltransferasas/química , Metiltransferasas/genética , Metiltransferasas/aislamiento & purificación , Nelumbo/química , Nelumbo/genética , Nelumbo/metabolismo , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/aislamiento & purificación , Alineación de Secuencia
3.
Comput Biol Chem ; 85: 107201, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-31986303

RESUMEN

In this study we isolated and performed in silico analysis of a putative coclaurine N-methyltransferase (CNMT) from the basal angiosperm Aristolochia fimbriata. The Aristolochiaceae plant family produces alkaloids similar to the Papavaraceae family, and CNMTs are central enzymes in biosynthesis pathways producing compounds of ethnopharmacological interest. We used bioinformatics and computational tools to predict a three-dimensional homology model and to investigate the putative function of the protein and its mechanism for methylation. The putative CNMT is a unique (S)-adenosyl-L-methionine (SAM)-dependent N-methyltransferase, catalyzing transfer of a methyl group from SAM to the amino group of coclaurine. The model revealed a mixed α/ß structure comprising seven twisted ß-strands surrounded by twelve α-helices. Sequence comparisons and the model indicate an N-terminal catalytic Core domain and a C-terminal domain, of which the latter forms a pocket for coclaurine. An additional binding pocket for SAM is connected to the coclaurine binding pocket by a small opening. CNMT activity is proposed to follow an SN2-type mechanism as observed for a similarly conformed enzyme. Residues predicted for the methyl transfer reaction are Tyr79 and Glu96, which are conserved in the sequence from A. fimbriata and in homologous N-methyltransferases. The isolated CNMT is the first to be investigated from any basal angiosperm.


Asunto(s)
Aristolochia/enzimología , Biología Computacional , Metiltransferasas/análisis , Metiltransferasas/aislamiento & purificación , Metiltransferasas/metabolismo , Modelos Moleculares , Conformación Proteica
4.
Methods Mol Biol ; 1405: 49-57, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-26843165

RESUMEN

Caffeine synthase (CS) is a methyltransferase responsible for the last two steps of the caffeine biosynthesis pathway in plants. CS is able to convert 7-methylxanthine to theobromine (3,7-dimethylxanthine) and theobromine to caffeine (1,3,7-trimethylxanthine) using S-adenosyl-L-methionine as the methyl donor in both reactions. The production of a recombinant protein is an important tool for the characterization of enzymes, particularly when the enzyme has affinity for different substrates. Guarana has the highest caffeine content among more than a hundred plant species that contain this alkaloid. Different from other plants, in which CS has a higher affinity for paraxanthine (1,7-dimethylxanthine), caffeine synthase from guarana (PcCS) has a higher affinity for theobromine. Here, we describe a method to produce a recombinant caffeine synthase from guarana in Escherichia coli and its purification by affinity chromatography. The recombinant protein retains activity and can be used in enzymatic assays and other biochemical characterization studies.


Asunto(s)
Escherichia coli/genética , Escherichia coli/metabolismo , Metiltransferasas/biosíntesis , Metiltransferasas/genética , Paullinia/genética , Proteínas Recombinantes , Cromatografía de Afinidad , Clonación Molecular , Metiltransferasas/aislamiento & purificación
5.
Plant Physiol ; 169(2): 1127-40, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26297140

RESUMEN

Transcriptome resources for the medicinal plant Glaucium flavum were searched for orthologs showing identity with characterized O-methyltransferases (OMTs) involved in benzylisoquinoline alkaloid biosynthesis. Seven recombinant proteins were functionally tested using the signature alkaloid substrates for six OMTs: norlaudanosoline 6-OMT, 6-O-methyllaudanosoline 4'-OMT, reticuline 7-OMT, norreticuline 7-OMT, scoulerine 9-OMT, and tetrahydrocolumbamine OMT. A notable alkaloid in yellow horned poppy (G. flavum [GFL]) is the aporphine alkaloid glaucine, which displays C8-C6' coupling and four O-methyl groups at C6, C7, C3', and C4' as numbered on the 1-benzylisoquinoline scaffold. Three recombinant enzymes accepted 1-benzylisoquinolines with differential substrate and regiospecificity. GFLOMT2 displayed the highest amino acid sequence identity with norlaudanosoline 6-OMT, showed a preference for the 6-O-methylation of norlaudanosoline, and O-methylated the 3' and 4' hydroxyl groups of certain alkaloids. GFLOMT1 showed the highest sequence identity with 6-O-methyllaudanosoline 4'OMT and catalyzed the 6-O-methylation of norlaudanosoline, but more efficiently 4'-O-methylated the GFLOMT2 reaction product 6-O-methylnorlaudanosoline and its N-methylated derivative 6-O-methyllaudanosoline. GFLOMT1 also effectively 3'-O-methylated both reticuline and norreticuline. GFLOMT6 was most similar to scoulerine 9-OMT and efficiently catalyzed both 3'- and 7'-O-methylations of several 1-benzylisoquinolines, with a preference for N-methylated substrates. All active enzymes accepted scoulerine and tetrahydrocolumbamine. Exogenous norlaudanosoline was converted to tetra-O-methylated laudanosine using combinations of Escherichia coli producing (1) GFLOMT1, (2) either GFLOMT2 or GFLOMT6, and (3) coclaurine N-methyltransferase from Coptis japonica. Expression profiles of GFLOMT1, GFLOMT2, and GFLOMT6 in different plant organs were in agreement with the O-methylation patterns of alkaloids in G. flavum determined by high-resolution, Fourier-transform mass spectrometry.


Asunto(s)
Aporfinas/metabolismo , Metiltransferasas/metabolismo , Papaveraceae/metabolismo , Proteínas de Plantas/metabolismo , Bencilisoquinolinas/metabolismo , Alcaloides de Berberina/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Regulación de la Expresión Génica de las Plantas , Isoquinolinas/metabolismo , Metiltransferasas/genética , Metiltransferasas/aislamiento & purificación , Papaveraceae/genética , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/aislamiento & purificación , Raíces de Plantas/metabolismo , Plantas Medicinales/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Especificidad por Sustrato , Tetrahidropapaverolina/metabolismo
6.
Antimicrob Agents Chemother ; 57(8): 3843-56, 2013 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-23733469

RESUMEN

The endosymbiotic organism Wolbachia is an attractive antifilarial drug target. Here we report on the cloning and expression of an rsmD-like rRNA methyltransferase from the Wolbachia endosymbiont of Brugia malayi, its molecular properties, and assays for specific inhibitors. The gene was found to be expressed in all the major life stages of B. malayi. The purified enzyme expressed in Escherichia coli was found to be in monomer form in its native state. The activities of the specific inhibitors (heteroaryl compounds) against the enzyme were tested with B. malayi adult and microfilariae for 7 days in vitro at various concentrations, and NSC-659390 proved to be the most potent compound (50% inhibitory concentration [IC50], 0.32 µM), followed by NSC-658343 (IC50, 4.13 µM) and NSC-657589 (IC50, 7.5 µM). On intraperitoneal administration at 5 mg/kg of body weight for 7 days to adult jirds into which B. malayi had been transplanted intraperitoneally, all the compounds killed a significant proportion of the implanted worms. A very similar result was observed in infected mastomys when inhibitors were administered. Docking studies of enzyme and inhibitors and an in vitro tryptophan quenching experiment were also performed to understand the binding mode and affinity. The specific inhibitors of the enzyme showed a higher affinity for the catalytic site of the enzyme than the nonspecific inhibitors and were found to be potent enough to kill the worm (both adults and microfilariae) in vitro as well as in vivo in a matter of days at micromolar concentrations. The findings suggest that these compounds be evaluated against other pathogens possessing a methyltransferase with a DPPY motif and warrant the design and synthesis of more such inhibitors.


Asunto(s)
Brugia Malayi/microbiología , Filaricidas/farmacología , Metiltransferasas/antagonistas & inhibidores , Metiltransferasas/aislamiento & purificación , Wolbachia/enzimología , Animales , Brugia Malayi/efectos de los fármacos , Brugia Malayi/genética , Clonación Molecular , Culicidae , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Inhibidores Enzimáticos/farmacología , Femenino , Filaricidas/administración & dosificación , Genes Bacterianos , Gerbillinae , Concentración 50 Inhibidora , Masculino , Metiltransferasas/genética , Metiltransferasas/metabolismo , Murinae , Especificidad por Sustrato , Simbiosis , Triptófano/metabolismo , Wolbachia/crecimiento & desarrollo
7.
J Bacteriol ; 194(16): 4161-8, 2012 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-22636775

RESUMEN

The genome of Methanosarcina acetivorans encodes three homologs, initially annotated as hypothetical fused corrinoid/methyl transfer proteins, which are highly elevated in CO-grown cells versus cells grown with alternate substrates. Based only on phenotypic analyses of deletion mutants, it was previously concluded that the homologs are strictly dimethylsulfide:coenzyme M (CoM) methyltransferases not involved in the metabolism of CO (E. Oelgeschlager and M. Rother, Mol. Microbiol. 72:1260 -1272, 2009). The homolog encoded by MA4383 (here designated CmtA) was reexamined via biochemical characterization of the protein overproduced in Escherichia coli. Purified CmtA reconstituted with methylcob(III)alamin contained a molar ratio of cobalt to protein of 1.0 ± 0.2. The UV-visible spectrum was typical of methylated corrinoid-containing proteins, with absorbance maxima at 370 and 420 nm and a band of broad absorbance between 450 and 600 nm with maxima at 525, 490, and 550 nm. CmtA reconstituted with aquocobalamin showed methyl-tetrahydromethanopterin:CoM (CH(3)-THMPT:HS-CoM) methyltransferase activity (0.31 µmol/min/mg) with apparent K(m) values of 135 µM for CH(3)-THMPT and 277 µM for HS-CoM. The ratio of CH(3)-THMPT:HS-CoM methyltransferase activity in the soluble versus membrane cellular fractions was 15-fold greater in CO-grown versus methanol-grown cells. A mutant strain deleted for the CmtA gene showed lower growth rates and final yields when cultured with growth-limiting partial pressures of CO, demonstrating a role for CmtA during growth with this substrate. The results establish that CmtA is a soluble CH(3)-THSPT:HS-CoM methyltransferase postulated to supplement the membrane-bound CH(3)-THMPT:HS-CoM methyltransferase during CO-dependent growth of M. acetivorans. Thus, we propose that the name of the enzyme encoded by MA4384 be CmtA (for cytoplasmic methyltransferase).


Asunto(s)
Monóxido de Carbono/metabolismo , Corrinoides/metabolismo , Methanosarcina/enzimología , Methanosarcina/crecimiento & desarrollo , Metiltransferasas/metabolismo , Clonación Molecular , Coenzimas/metabolismo , Escherichia coli/genética , Eliminación de Gen , Expresión Génica , Cinética , Mesna/metabolismo , Methanosarcina/metabolismo , Metiltransferasas/genética , Metiltransferasas/aislamiento & purificación , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Espectrofotometría Ultravioleta , Vitamina B 12/análogos & derivados , Vitamina B 12/metabolismo
8.
Biosci Biotechnol Biochem ; 75(1): 107-13, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-21228475

RESUMEN

Carapichea ipecacuanha produces various emetine-type alkaloids, known as ipecac alkaloids, which have long been used as expectorants, emetics, and amebicides. In this study, we isolated an O-methyltransferase cDNA from this medicinal plant. The encoded protein (CiOMT1) showed 98% sequence identity to IpeOMT2, which catalyzes the 7'-O-methylation of 7'-O-demethylcephaeline to form cephaeline at the penultimate step of emetine biosynthesis (Nomura and Kutchan, J. Biol. Chem., 285, 7722-7738 (2010)). Recombinant CiOMT1 showed both 7'-O-methylation and 6'-O-methylation activities at the last two steps of emetine biosynthesis. This indicates that small differences in amino acid residues are responsible for distinct regional methylation specificities between IpeOMT2 and CiOMT1, and that CiOMT1 might contribute to two sequential O-methylation steps from 7'-O-demethylcephaeline to emetine.


Asunto(s)
Metiltransferasas/genética , Raíces de Plantas/enzimología , Rubiaceae/enzimología , Alcaloides/biosíntesis , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , ADN Complementario/genética , Escherichia coli/genética , Evolución Molecular , Metiltransferasas/biosíntesis , Metiltransferasas/química , Metiltransferasas/aislamiento & purificación , Datos de Secuencia Molecular , Raíces de Plantas/genética , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Rubiaceae/genética , Especificidad por Sustrato
9.
Insect Biochem Mol Biol ; 34(8): 785-98, 2004 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-15262283

RESUMEN

Methyl farnesoate (MF) appears to have important roles in the development, morphogenesis, and reproduction of crustaceans. To better understand the regulation of MF synthesis, we studied farnesoic acid O-methyltransferase (FAOMeT, the final enzyme in the MF biosynthetic pathway) in the American lobster (Homarus americanus). FAOMeT purified from mandibular organ (MO) homogenates had a MW of approximately 38,000. The sequences of trypsin fragments of purified FAOMeT were used to design PCR primers to amplify a cDNA fragment, which was used to isolate a full-length cDNA containing a single open reading frame (ORF) of 828 bp encoding a protein of 276 amino acids. The deduced amino acid sequence of this putative FAOMeT protein contained two copies of a conserved approximately 135 amino acid domain we term the CF (CPAMD8/FAOMeT) domain; single copies of this domain also occur in the human CPAMD8 protein (a member of the alpha-2 macroglobulin family) and an uncharacterized Drosophila protein. The recombinant protein had no FAOMeT activity. However, its addition to MO homogenates from eyestalk ablated (ESA) lobsters increased enzyme activity by up to 75%, suggesting that FAOMeT may require an additional factor or modification (e.g., phosphorylation) for its activation. The mRNA for the putative FAOMeT was primarily found in the proximal region of the MO, the predominant site of MF synthesis. FAOMeT transcripts were found in muscle tissue from ESA animals, but not in green gland, hepatopancreas, or in muscle tissue from intact animals. FAOMeT mRNA was also detected in embryos and larval stages. This is the first comprehensive report of this protein in the lobster, and is an important step in elucidating the functions of MF in these animals.


Asunto(s)
Mandíbula/enzimología , Metiltransferasas/genética , Metiltransferasas/aislamiento & purificación , Nephropidae/enzimología , Nephropidae/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Embrión no Mamífero/enzimología , Embrión no Mamífero/metabolismo , Escherichia coli , Larva/enzimología , Larva/genética , Mandíbula/metabolismo , Metiltransferasas/biosíntesis , Metiltransferasas/metabolismo , Datos de Secuencia Molecular , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Análisis de Secuencia de ADN
10.
Phytochemistry ; 64(2): 453-8, 2003 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-12943762

RESUMEN

S-Adenosyl-L-methionine:beta-peltatin 6-O-methyltransferase was isolated and characterized from cell suspension cultures of Linum nodiflorum L. (Linaceae), a Linum species accumulating aryltetralin lignans such as 6-methoxypodophyllotoxin. The enzyme transfers a methyl group from S-adenosyl-L-methionine to the only free OH-group of beta-peltatin in position 6 thus forming beta-peltatin-A methylether. This reaction is a putative biosynthetic step in the biosynthesis of 6-methoxypodophyllotoxin from deoxypodophyllotoxin. The enzyme has a pH-optimum at pH 7.7 and a temperature optimum at 40 degrees C. The enzyme activity is strongly inhibited by MnSO(4), FeCl(3), FeSO(4) and ZnSO(4) as well as S-adenosyl-homocysteine. Mg(2+) and EDTA did not influence the methylation of beta-peltatin. Substrate saturation curves were obtained for S-adenosyl-methionine and beta-peltatin and apparent K(m)-values of 15 microM and 40 microM, respectively, were determined for these substrates. Substrate inhibition was observed for beta-peltatin. No other lignan substrate tested nor caffeic acid were accepted. The suspension cell line of Linum nodiflorum was characterized with respect to growth, medium alterations and lignan production as well as activity of SAM:beta-peltatin 6-O-methyltransferase. Highest specific activities of beta-peltatin 6-O-methyltransferase were determined on day 7 of the culture period corresponding to the highest levels of 6-methoxypodophyllotoxin on days 7 to 12.


Asunto(s)
Lino/enzimología , Metiltransferasas/aislamiento & purificación , Metiltransferasas/metabolismo , Podofilotoxina/análogos & derivados , Podofilotoxina/metabolismo , Medicamentos Herbarios Chinos , Inhibidores Enzimáticos/farmacología , Lino/citología , Lino/crecimiento & desarrollo , Concentración de Iones de Hidrógeno , Lignanos/análisis , Lignanos/biosíntesis , Metiltransferasas/antagonistas & inhibidores , Podofilotoxina/química , Podofilotoxina/farmacología , S-Adenosilmetionina/metabolismo , Especificidad por Sustrato , Temperatura , Factores de Tiempo
11.
Phytochemistry ; 56(7): 649-55, 2001 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-11314949

RESUMEN

S-Adenosyl-L-methionine (SAM): coclaurine N-methyltransferase (CNMT), which catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the amino group of the tetrahydrobenzylisoquinoline alkaloid coclaurine. was purified 340-fold from Coptis japonica cells in 1% yield to give an almost homogeneous protein. The purified enzyme, which occurred as a homotetramer with a native Mr of 160 kDa (gel-filtration chromatography) and a subunit Mr of 45 kDa (SDS-polyacrylamide gel electrophoresis), had an optimum pH of 7.0 and a pI of 4.2. Whereas (R)-coclaurine was the best substrate for enzyme activity, Coptis CNMT had broad substrate specificity and no stereospecificity CNMT methylated norlaudanosoline, 6,7-dimethoxyl-1,2,3,4-tetrahydroisoquinoline and 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline. The enzyme did not require any metal ion. p-Chloromercuribenzoate and iodoacetamide did not inhibit CNMT activity, but the addition of Co2+, Cu2+ or Mn2+ at 5 mM severely inhibited such activity by 75, 47 and 57%, respectively. The substrate-saturation kinetics of CNMT for norreticuline and SAM were of the typical Michaelis-Menten-type with respective Km values of 0.38 and 0.65 mM.


Asunto(s)
Metiltransferasas/aislamiento & purificación , Metiltransferasas/metabolismo , Plantas Medicinales/enzimología , Alcaloides/metabolismo , Células Cultivadas , Cromatografía en Gel , Cromatografía por Intercambio Iónico , Isoquinolinas/metabolismo , Cinética , Metiltransferasas/química , Peso Molecular , Plantas Medicinales/citología , Estereoisomerismo , Especificidad por Sustrato
12.
Mol Cell ; 8(6): 1207-17, 2001 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-11779497

RESUMEN

Methylation of histone H3 at lysine 9 by SUV39H1 and subsequent recruitment of the heterochromatin protein HP1 has recently been linked to gene silencing. In addition to lysine 9, histone H3 methylation also occurs at lysines 4, 27, and 36. Here, we report the purification, molecular identification, and functional characterization of an H3-lysine 4-specific methyltransferase (H3-K4-HMTase), SET7. We demonstrate that SET7 methylates H3-K4 in vitro and in vivo. In addition, we found that methylation of H3-K4 and H3-K9 inhibit each other. Furthermore, H3-K4 and H3-K9 methylation by SET7 and SUV39H1, respectively, have differential effects on subsequent histone acetylation by p300. Thus, our study provides a molecular explanation to the differential effects of H3-K4 and H3-K9 methylation on transcription.


Asunto(s)
N-Metiltransferasa de Histona-Lisina , Histonas/química , Histonas/metabolismo , Lisina/metabolismo , Metiltransferasas/metabolismo , Acetilación , Acetiltransferasas/metabolismo , Secuencia de Aminoácidos , Animales , Unión Competitiva , Proteínas de Ciclo Celular/metabolismo , ADN Complementario/genética , Silenciador del Gen , Células HeLa , Heterocromatina/química , Heterocromatina/metabolismo , Histona Acetiltransferasas , Histona Metiltransferasas , Humanos , Metilación , Metiltransferasas/química , Metiltransferasas/genética , Metiltransferasas/aislamiento & purificación , Ratones , Datos de Secuencia Molecular , Peso Molecular , Nucleosomas/química , Nucleosomas/metabolismo , Proteína Metiltransferasas , Estructura Terciaria de Proteína , Especificidad por Sustrato , Factores de Transcripción , Factores de Transcripción p300-CBP
13.
Plant Physiol ; 121(1): 281-90, 1999 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-10482684

RESUMEN

The transfer of a methyl group from S-adenosyl-L-methionine onto the carboxyl group of alpha-1,4-linked-galactosyluronic acid residues in the pectic polysaccharide homogalacturonan (HGA) is catalyzed by an enzyme commonly referred to as pectin methyltransferase. A pectin methyltransferase from microsomal membranes of tobacco (Nicotiana tabacum) was previously characterized (F. Goubet, L.N. Council, D. Mohnen [1998] Plant Physiol 116: 337-347) and named HGA methyltransferase (HGA-MT). We report the solubilization of HGA-MT from tobacco membranes. Approximately 22% of the HGA-MT activity in total membranes was solubilized by 0.65% (w/v) 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid containing 1 mM dithioerythritol. The addition of phosphatidylcholine and the methyl acceptors HGA or pectin (30% degree of esterification) to solubilized enzyme increased HGA-MT activity to 35% of total membrane-bound HGA-MT activity. Solubilized HGA-MT has a pH optimum of 7.8, an apparent K(m) for S-adenosyl-L-methionine of 18 microM, and an apparent V(max) of 0. 121 pkat mg(-1) of protein. The apparent K(m) for HGA and for pectin is 0.1 to 0.2 mg mL(-1). Methylated product was solubilized with boiling water and ammonium oxalate, two conditions used to solubilize pectin from the cell wall. The release of 75% to 90% of the radioactivity from the product pellet by mild base treatment showed that the methyl group was incorporated as a methyl ester rather than a methyl ether. The fragmentation of at least 55% to 70% of the radiolabeled product by endopolygalacturonase, and the loss of radioactivity from the product by treatment with pectin methylesterase, demonstrated that the bulk of the methylated product produced by the solubilized enzyme was pectin.


Asunto(s)
Membranas Intracelulares/enzimología , Metiltransferasas/aislamiento & purificación , Metiltransferasas/metabolismo , Microsomas/enzimología , Nicotiana/enzimología , Pectinas/metabolismo , Plantas Tóxicas , Hidrolasas de Éster Carboxílico/metabolismo , Cationes Bivalentes/farmacología , Células Cultivadas , Ácidos Cólicos/metabolismo , Ditioeritritol , Activación Enzimática/efectos de los fármacos , Estabilidad de Enzimas , Concentración de Iones de Hidrógeno , Cinética , Metilación , Metiltransferasas/antagonistas & inhibidores , Oxalatos/metabolismo , Poligalacturonasa/metabolismo , Solubilidad , Temperatura , Nicotiana/citología , Agua/metabolismo
14.
Plant Physiol ; 120(2): 579-86, 1999 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-10364410

RESUMEN

Caffeine synthase (CS), the S-adenosylmethionine-dependent N-methyltransferase involved in the last two steps of caffeine biosynthesis, was extracted from young tea (Camellia sinensis) leaves; the CS was purified 520-fold to apparent homogeneity and a final specific activity of 5.7 nkat mg-1 protein by ammonium sulfate fractionation and hydroxyapatite, anion-exchange, adenosine-agarose, and gel-filtration chromatography. The native enzyme was monomeric with an apparent molecular mass of 61 kD as estimated by gel-filtration chromatography and 41 kD as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme displayed a sharp pH optimum of 8.5. The final preparation exhibited 3- and 1-N-methyltransferase activity with a broad substrate specificity, showing high activity toward paraxanthine, 7-methylxanthine, and theobromine and low activity with 3-methylxanthine and 1-methylxanthine. However, the enzyme had no 7-N-methyltransferase activity toward xanthosine and xanthosine 5'-monophosphate. The Km values of CS for paraxanthine, theobromine, 7-methylxanthine, and S-adenosylmethionine were 24, 186, 344, and 21 microM, respectively. The possible role and regulation of CS in purine alkaloid biosynthesis in tea leaves are discussed. The 20-amino acid N-terminal sequence for CS showed little homology with other methyltransferases.


Asunto(s)
Cafeína/biosíntesis , Metiltransferasas/aislamiento & purificación , Té/enzimología , Marcadores de Afinidad , Secuencia de Aminoácidos , Electroforesis en Gel de Poliacrilamida , Concentración de Iones de Hidrógeno , Cinética , Metiltransferasas/genética , Metiltransferasas/metabolismo , Datos de Secuencia Molecular , Peso Molecular , Hojas de la Planta/enzimología , Especificidad por Sustrato , Té/genética
15.
Phytochemistry ; 45(7): 1407-14, 1997 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-9237402

RESUMEN

Caffeine biosynthesis comprises sequential methylations at N-7, N-3 and N-1 of the xanthine ring catalysed by S-adenosyl-L-methionine (SAM)-dependent methyltransferase activities that, to date, have not been resolved. Enzyme extracts were prepared from young, emerging coffee leaflets and following anion exchange chromatography, chromatofocusing facilitated the clear separation of the N-7-methyltransferase from the N-3- and N-1-methyltransferase activities. All three N-methyltransferases co-eluted when analysed by gel filtration chromatography and their native molecular mass was ca 67 kDa. Photoaffinity labelling with [methyl-3H]SAM followed by SDS-PAGE of a chromatofocusing-purified preparation containing only N-7-methyltransferase activity demonstrated the presence of a single labelled band of 40 kDa. Similar analysis of a gel filtration purified preparation containing all three N-methyltransferase activities revealed the presence of three labelled bands at 49, 43 and 40 kDa. It remains to be determined whether the 49 and 43 kDa bands are associated with the N-3 and N-1-methyltransferases or whether they are unrelated SAM-dependent methyltransferases or other SAM-binding proteins.


Asunto(s)
Cafeína/biosíntesis , Café/enzimología , Metiltransferasas/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación , Proteínas de Plantas/aislamiento & purificación , Marcadores de Afinidad/metabolismo , Marcadores de Afinidad/farmacología , Cromatografía de Afinidad , Metiltransferasas/metabolismo , Extractos Vegetales/metabolismo , Hojas de la Planta/enzimología , Proteínas de Plantas/metabolismo , S-Adenosilmetionina/metabolismo , S-Adenosilmetionina/farmacología , Sensibilidad y Especificidad , Tritio
16.
J Biol Chem ; 272(5): 2744-52, 1997 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-9006913

RESUMEN

Siroheme, the prosthetic group for both nitrite and sulfite reductases, is a methylated, iron-containing modified tetrapyrrole. Here we report the first molecular characterization of the branch point enzyme in higher plants, which directs intermediates toward siroheme synthesis. A cDNA was cloned from Arabidopsis thaliana (UPM1) that functionally complements an Escherichia coli cysG mutant, a strain that is unable to catalyze the conversion of uroporphyrinogen III (Uro'gen-III) to siroheme. UPM1 is 1484 base pairs and encodes a 369-amino acid, 39.9-kDa protein. The UPM1 product contains two regions that are identical to consensus sequences found in bacterial Uro'gen-III and precorrin methyltransferases. Recombinant UPM1 protein was found to catalyze S-adenosyl-L-methionine-dependent transmethylation by UPM1 in a multistep process involving the formation of a covalently linked complex with S-adenosyl-L-methionine. The UPM1 product has a sequence at the amino terminus that resembles a transit peptide for localization to mitochondria or plastids. The protein produced by in vitro expression is able to enter isolated intact chloroplasts but not mitochondria. Genomic blot analysis showed that UPM1 is encoded in the A. thaliana genome. The genomic DNA corresponding to UPM1 was cloned and sequenced and found to contain at least five introns.


Asunto(s)
Arabidopsis/enzimología , Hemo/análogos & derivados , Metiltransferasas/metabolismo , Secuencia de Aminoácidos , Arabidopsis/genética , Secuencia de Bases , Mapeo Cromosómico , Clonación Molecular , Cartilla de ADN , ADN Complementario , Escherichia coli , Genes de Plantas , Hemo/biosíntesis , Cinética , Metiltransferasas/química , Metiltransferasas/aislamiento & purificación , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Proteínas Recombinantes/química , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , S-Adenosilmetionina/metabolismo , Homología de Secuencia de Aminoácido , Espectrofotometría
17.
J Biol Chem ; 271(37): 22831-8, 1996 Sep 13.
Artículo en Inglés | MEDLINE | ID: mdl-8798461

RESUMEN

Porcine liver betaine-homocysteine methyltransferase (BHMT; EC) was purified to homogeneity, and the Michaelis constants for betaine, dimethylacetothetin, and L-homocysteine are 23, 155, and 32 microM, respectively. The maximum rate of catalysis is 47-fold greater using dimethylacetothetin as a methyl donor compared with betaine. Partial amino acid sequence of porcine BHMT was obtained, and inosine-containing redundant oligonucleotide primers were used to amplify an 815-base pair sequence of the porcine cDNA by polymerase chain reaction (PCR). Nondegenerate oligonucleotide primers based on the porcine cDNA were synthesized and used to isolate a 463-base pair fragment of the human cDNA by PCR. The human PCR DNA product was then used to screen a cDNA library by plaque hybridization, and cDNAs encoding human BHMT were isolated. The primary structure of the human cDNA is reported here, and the open reading frame encodes a 406-residue protein of Mr 44,969. The deduced amino acid sequence of human BHMT shows limited homology to bacterial vitamin B12-dependent methionine synthases (EC). A plasmid containing the human BHMT cDNA fused in frame to the N terminus of beta-galactosidase was transformed into Escherichia coli, and transformants expressed BHMT activity, an activity that is absent from wild type E. coli.


Asunto(s)
Metiltransferasas/aislamiento & purificación , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Betaína-Homocisteína S-Metiltransferasa , Clonación Molecular , ADN Complementario , Humanos , Cinética , Hígado/enzimología , Metiltransferasas/genética , Metiltransferasas/metabolismo , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Proteínas Recombinantes de Fusión/química , Porcinos
18.
J Biol Chem ; 270(39): 22968-73, 1995 Sep 29.
Artículo en Inglés | MEDLINE | ID: mdl-7559434

RESUMEN

Although Rhizobium sp. NGR234 and Rhizobium fredii USDA257 share many traits, dysfunctional nodSU genes in the latter prohibit nodulation of Leucaena species. Accordingly, we used R. fredii transconjugants harboring the nodS and nodU genes of NGR234 to study their role in the structural modification of the lipo-oligosaccharide Nod factors. Differences between the Nod factors mainly concern the length of the oligomer (three to five glucosamine residues in USDA257 and five residues only in NGR234) and the presence of additional substituents in NGR234 (N-linked methyl, one or two carbamoyl groups on the non-reducing moiety, acetyl or sulfate groups on the fucose). R. fredii(nodS) transconjugants produce chitopentamer Nod factors with a N-linked methyl group on the glucosaminyl terminus. Introduction of nodU into USDA257 results in the formation of 6-O-carbamoylated factors. Co-transfer of nodSU directs N-methylation, mono-6-O-carbamoylation, and production of pentameric Nod factors. Mutation of nodU in NGR234 suppresses the formation of bis-carbamoylated species. Insertional mutagenesis of nodSU drastically decreases Nod factor production, but with the exception of sulfated factors (which are partially N-methylated and mono-carbamoylated), they are identical to those of the wild-type strain. Thus, Nod factor levels, their degree of oligomerization, and N-methylation are linked to the activity encoded by nodS.


Asunto(s)
Proteínas Bacterianas/metabolismo , Transferasas de Carboxilo y Carbamoilo , Genes Bacterianos , Lipopolisacáridos/biosíntesis , Metiltransferasas/metabolismo , Rhizobium/genética , Rhizobium/metabolismo , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/aislamiento & purificación , Secuencia de Carbohidratos , Células Cultivadas , Conjugación Genética , Fabaceae/microbiología , Glucosamina/análisis , Glucosamina/metabolismo , Lipopolisacáridos/química , Solanum lycopersicum , Metilación , Metiltransferasas/biosíntesis , Metiltransferasas/aislamiento & purificación , Datos de Secuencia Molecular , Plantas Medicinales , Plásmidos , Regiones Promotoras Genéticas , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/metabolismo , Mapeo Restrictivo , Simbiosis
19.
Eur J Biochem ; 225(1): 125-31, 1994 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-7925429

RESUMEN

S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase (norcoclaurine 6-O-methyltransferase), which catalyzes the transfer of the S-methyl group of S-adenosyl-L-methionine to the 6-hydroxyl group of 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl)methyl]-6,7- isoquinolinediol (norcoclaurine), was purified from cultured Coptis japonica cells and its enzymic properties were characterized. Purified norcoclaurine 6-O-methyltransferase had apparent pI 4.7, a native molecular mass of 95 kDa (determined by gel filtration) and subunit molecular mass of 40 kDa (SDS/PAGE). The enzyme did not require a divalent cation for activity, and the addition of Fe2+, Cu2+, Co2+, Zn2+, Mn2+, or Ni2+ at 5 mM severely inhibited enzyme activity. Neither p-chloromercuribenzoate, N-methylmaleimide nor iodoacetamide inhibited enzyme activity at 1 mM. 5,6-Dihydro-9,10-dimethoxybenzo[g]-1,3-benzodioxolo[5,6-a]qu inolizinium (berberine, the end-product of the biosynthetic pathway in which norcoclurine 6-O-methyltransferase catalyzes an intermediate step) also inhibited the activity by 50% at 10 mM. Norcoclaurine 6-O-methyltransferase methylated both (S)-norcoclaurine and (R)-norcoclaurine and (R,S)-norlaudanosoline. Further characterization of substrate-saturation kinetics and product inhibition of the purified enzyme indicated that norcoclaurine 6-O-methyltransferase follows a bi-bi ping-pong mechanism with Km values of 2.23 mM and 3.95 mM for (R,S)-norlaudanosoline and S-adenosyl-L-methionine, respectively, while Ki values for S-adenosylhomocysteine versus S-adenosyl-L-methionine and (R,S)-norlaudanosoline were 2.1 mM and 0.18 mM, respectively.


Asunto(s)
Metiltransferasas/aislamiento & purificación , Metiltransferasas/metabolismo , Plantas Medicinales/enzimología , Secuencia de Aminoácidos , Células Cultivadas , Cromatografía , Cromatografía por Intercambio Iónico , Durapatita , Cinética , Metiltransferasas/química , Datos de Secuencia Molecular , Peso Molecular , Fragmentos de Péptidos/química , Fragmentos de Péptidos/aislamiento & purificación , Especificidad por Sustrato
20.
Arch Biochem Biophys ; 302(1): 222-7, 1993 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-8470900

RESUMEN

In the presence of rat liver cytosol and homocysteine, trimethylselenonium ion (TMSe+) underwent time-dependent demethylation to dimethylselenide with the concurrent formation of methionine. Convenient methods were developed for assay of this activity using either radioactive methods based on the gamma emitting isotope 75Se or nonradioactive HPLC assay of methionine. The rate of demethylation was linear with protein concentration and dependent on homocysteine, which could not be replaced by cysteine, glutathione, or dithiothreitol. The TMSe+ demethylation rate was inhibited by the addition of betaine, sulfobetaine (dimethylthetin), or dimethylglycine. The Km for TMSe+ was 8 mM compared to 0.04 mM for betaine, but the rate of TMSe+ demethylation was approximately 50-fold that of betaine when both were assayed at 25 mM. Methionine was also produced from selenobetaine, selenobetaine methylester, and sulfobetaine. The selenium analogues of betaine inhibited the demethylation of TMSe+ with only minor decreases in methionine production, indicating substrate competition. In preliminary studies aimed at the partial purification of the TMSe+:homocysteine methyltransferase activity, the enzyme was found to have chromatographic and heat stability characteristics similar to betaine:homocysteine methyltransferase. The data indicate that betaine:homocysteine methyltransferase, or a very similar enzyme, is involved in the demethylation of TMSe+ and show that TMSe+, an in vivo urinary selenium metabolite of many selenium compounds, is not biologically inert.


Asunto(s)
Betaína/análogos & derivados , Homocisteína/farmacología , Metionina/metabolismo , Compuestos de Organoselenio/metabolismo , Compuestos de Selenio , Selenio/metabolismo , Animales , Betaína/metabolismo , Unión Competitiva , Citosol/enzimología , Homocisteína S-Metiltransferasa , Cinética , Hígado/enzimología , Masculino , Metilación , Metiltransferasas/aislamiento & purificación , Metiltransferasas/metabolismo , Ratas , Ratas Sprague-Dawley
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