RESUMEN
Un the basis of morphological studies examined the effectiveness of complex pathogenetic treatment of generalized periodontal disease with the use of products based on natural ingredients: dental paste "Fitopasta-3K" and drug "plantain juice" for the local treatment and drugs "Granules quercetin" and "Energoton" for systemic treatment. Established that one of the important mechanisms of complex pathogenetic treatment is the effect on energy metabolism of periodontal tissues, including the reduction of tissue hypoxia, metabolic stabilization was observed structural changes navkolozubnyh tissues. It should be noted positive effects of integrated treatment on the whole body.
Asunto(s)
Enfermedad Coronaria/complicaciones , Enfermedades Periodontales/rehabilitación , Periodoncio/patología , Preparaciones de Plantas/uso terapéutico , Enfermedad Coronaria/metabolismo , Enfermedad Coronaria/patología , Enfermedad Coronaria/rehabilitación , Quimioterapia Combinada , Metabolismo Energético/efectos de los fármacos , Humanos , Consumo de Oxígeno/efectos de los fármacos , Enfermedades Periodontales/complicaciones , Enfermedades Periodontales/metabolismo , Enfermedades Periodontales/patología , Periodoncio/irrigación sanguínea , Periodoncio/efectos de los fármacos , Periodoncio/enzimología , Preparaciones de Plantas/administración & dosificación , Resultado del TratamientoRESUMEN
OBJECTIVE: To investigate the influence of Bushenguchiwan on expression of matrix metalloproteinase-13 (MMP-13) in periodontium of rats with experimental periodontitis. METHODS: The model of experimental periodontitis of rats was established and treated by Bushenguchiwan with different doses. The periodontal tissues from groups of different doses were immunohistochemically stained by antibody of MMP-13. The expression of MMP-13 was examined and semi-quantitative analysis of signals performed by integrated absorbance. RESULTS: MMP-13 was intensely positive in gingival epithelial cells and periodontal fibroblasts in periodontitis models and negative in normal rat periodontal tissues. After 30 days of Bushenguchiwan treatment with high dose, middle dose and low dose, the expression of MMP-13 (2.9103 ± 0.5534, 3.6588 ± 0.4330, 4.4550 ± 0.4255) was down-regulated respectively compared with model rats (5.3233 ± 0.7993), P < 0.05. After 60 days of treatment the expression of MMP-13 (2.1855 ± 0.5381, 2.8558 ± 0.4759, 3.8980 ± 0.5885) was down-regulated more significantly. with model rats (6.2693 ± 0.4538), P < 0.05. CONCLUSIONS: Bushenguchiwan could down-regulate the expression of MMP-13 in rats' periodontium and the high dose group had better effect.
Asunto(s)
Medicamentos Herbarios Chinos/uso terapéutico , Metaloproteinasa 13 de la Matriz/metabolismo , Periodontitis/enzimología , Periodoncio/enzimología , Animales , Relación Dosis-Respuesta a Droga , Medicamentos Herbarios Chinos/administración & dosificación , Células Epiteliales/enzimología , Femenino , Fibroblastos/enzimología , Encía/citología , Encía/enzimología , Masculino , Periodontitis/tratamiento farmacológico , Periodontitis/microbiología , Periodoncio/citología , Porphyromonas gingivalis , Distribución Aleatoria , Ratas , Ratas Sprague-DawleyRESUMEN
This study observed the effect of Gu Chi Wan on the enzyme histochemistry changes of diabetic rats with experimental periodontal disease. The results indicate that the experimental group has not the decrease of oxidase-reductase and alkaline phosphatase as seen in control group and demonstrate the protective effect of Gu Chi Wan perhaps acts through regulating metabolism of periodontium of rats with experimental periodontal disease, and then improving the immune response of the host.