RESUMEN
Antibiotics are still the primary therapy for acute pyelonephritis (APN); rarely, natural polyphenols are also used. LM49 is a novel marine bromophenol derivative displaying strong anti-inflammatory effects. We investigated the therapeutic efficacy of LM49 in an experimental rat model of APN. The model was established by injecting 0.5â¯mL Escherichia coli (ATCC 25922, 108â¯CFU/mL) into the urinary bladders of Sprague Dawley rats. This model showed increased kidney viscera indices and renal bacterial growth scores, as well as pathological changes in kidneys. We also performed a broth microdilution antimicrobial susceptibility test of the E. coli strain. Both norfloxacin and LM49 treatment reduced kidney viscera indices and decreased microbial counts in urine cultures and kidney homogenates in APN rats. However, in vitro experiments showed that LM49 did not directly inhibit bacteria. Rather, LM49 treatment inhibited inflammatory cell infiltration or abscess and improved tissue lesions in the renal medullary junction, renal pelvis, and calyx, and high-dose LM49 treatment inhibited the production of inflammatory interleukin-1ß (IL-1ß) and interleukin-6 (IL-6) in serum. CD4+ T cells were higher in the LM49 groups treated with high, medium, and low doses than in the model group, whereas only high-dose LM49 treatment increased the number of CD8+ T cells, as compared with that in the model group. However, LM49 treatment did not influence hematological parameters. Our results show that LM49 therapeutic effects in an APN animal model may be achieved by regulating immune responses and inhibiting inflammatory mediators, suggesting it as a candidate APN treatment.
Asunto(s)
Antibacterianos/uso terapéutico , Antiinflamatorios/uso terapéutico , Benzofenonas/uso terapéutico , Infecciones por Escherichia coli/tratamiento farmacológico , Inmunomodulación/efectos de los fármacos , Fenoles/uso terapéutico , Pielonefritis/tratamiento farmacológico , Enfermedad Aguda , Animales , Citocinas/sangre , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Infecciones por Escherichia coli/sangre , Infecciones por Escherichia coli/inmunología , Riñón/efectos de los fármacos , Riñón/microbiología , Riñón/patología , Masculino , Pruebas de Sensibilidad Microbiana , Pielonefritis/sangre , Pielonefritis/inmunología , Ratas Sprague-Dawley , Orina/microbiologíaRESUMEN
This review article presents the data on the mechanism of action of peloid therapy from the perspective of its defibrosing effect, the structure and functions of the extracellular matrix under the normal and pathological conditions. In addition, role of this treatment modality in the progression of tubulointerstitial fibrosis which determines the severity and prognosis of chronic pyelonephritis is considered. The researchers are currently carry out the extensive studies aimed at the search of the methods for the primary and secondary prevention of chronic pyelonephritis. A wide range of pharmacotherapeutic modalities are currently used for this purpose. Moreover the development of long-acting anti-relapse medications is currently underway along with the further improvement of high-tech reconstructive surgical methods for the intervention on the organs of the urinary system. At the same time, the nephroprotective potential of the natural physical factors, such as therapeutic muds, e.g. peloids, remains poorly explored even though their well apparent thermophysical properties, unique organic and mineral composition, and saturation with biologically active compounds are well known long ago. The systemic response to peloid therapy manifests itself as the changes in the metabolism of the intercellular matrix and collagen of the connective tissue associated with the alterations in the process of fibrogenesis and the development of tubulointerstitial disorders. The direct and indirect influence of peloids on the connective tissue is possible. The indirect effects are attributable to the peloid impact on the antioxidant status, immunity, hormonal regulation, and metabolic processes. These findings suggest the necessity of the relevant experimental and clinical studies for the evaluation of the influence of peloid therapy on the structure and metabolism of the connective tissue in the kidneys including dynamics of the markers of inflammation, proliferation and fibrogenesis, and the hormonal status of the patients suffering from chronic pyelonephritis based on the application of the modern technologies in accordance with the requirements of evidence-based medicine.
Asunto(s)
Peloterapia/métodos , Pielonefritis/rehabilitación , Prevención Secundaria/métodos , Enfermedad Crónica , Humanos , Pielonefritis/inmunología , Pielonefritis/patología , Resultado del TratamientoRESUMEN
The present paper reports results of a comparative study of 48 patients with latent chronic pyelonephritis and 3-18 years of duration of the disease. Patients assigned to group 1 (n=23) received standard treatment. In patients of group 2 (n=25) standard treatment was supplemented by the administration of 30-day course of Kanferon N 2 dragees twice daily. Kanferon N supplementation was found to be effective as an anti-inflammatory-measure due to its positive impact on renal microcirculation and urinary bacterial composition. It reduces the level of proinflammatory cytokines, stimulates humoral and cellular immunity thus preventing further disease progression.
Asunto(s)
Fitoterapia , Pielonefritis/tratamiento farmacológico , Adolescente , Niño , Preescolar , Enfermedad Crónica , Citocinas/inmunología , Femenino , Humanos , Inmunidad Celular/efectos de los fármacos , Inmunidad Humoral/efectos de los fármacos , Riñón/irrigación sanguínea , Riñón/inmunología , Riñón/patología , Masculino , Microcirculación/efectos de los fármacos , Pielonefritis/inmunología , Pielonefritis/fisiopatologíaRESUMEN
The purpose of this study was to increase the effectiveness of treatment of patients with acute pyelonephritis by incorporating of phytopreparation "Prolit Super Septo" in complex therapy. The study included 38 patients with acute primary pyelonephritis. All patients have received standard medical therapy, 21 of them (Group 2) have additionally received "Prolit Super Septo" 2 capsules 2 times a day for 1 month. Outcome measures included basic laboratory parameters, and results of bacteriological examination and immunological assessment of patients 12-14 and 28-30 days after initiation of therapy. Against the background of the therapy, positive dynamics of the level of leukocyturia and bacteriuria was detected in both groups; therapeutic effect, however, was more pronounced in the Group 2 treated with complex therapy: after the end of treatment, the proportion of sterile cultures was 85.7%, whereas in Group 1--70.6%. In addition, complex treatment had a positive effect on the immune status of patients, as evidenced by normalization of blood immunoglobulins, circulating immune complexes and C-reactive protein. The inclusion of "Prolit Super Septo" in the complex conservative treatment of patients with acute pyelonephritis promotes the development of an adequate immune response and the rapid elimination of the pathogens, thus preventing the development of purulent forms of the disease, or chronization of process.
Asunto(s)
Antibacterianos/uso terapéutico , Fitoterapia/métodos , Preparaciones de Plantas/uso terapéutico , Pielonefritis/tratamiento farmacológico , Pielonefritis/inmunología , Enfermedad Aguda , Adolescente , Adulto , Antibacterianos/administración & dosificación , Quimioterapia Combinada , Femenino , Humanos , Persona de Mediana Edad , Preparaciones de Plantas/administración & dosificación , Pielonefritis/diagnóstico , Resultado del Tratamiento , Adulto JovenRESUMEN
Studies have shown that complicated chronic pyelonephritis in the active phase is characterized by structural and functional instability cytomembranes and decreased immunological resistance of the patient man. Supplement standard antibiotic treatment with ozone therapy antioxidant immunomodulation drug thus received immunobiochemical study.
Asunto(s)
Antibacterianos/uso terapéutico , Antioxidantes/uso terapéutico , Luminol/análogos & derivados , Ozono/uso terapéutico , Pielonefritis , Antibacterianos/administración & dosificación , Antioxidantes/administración & dosificación , Enfermedad Crónica , Terapia Combinada , Quimioterapia Combinada , Membrana Eritrocítica/efectos de los fármacos , Membrana Eritrocítica/enzimología , Membrana Eritrocítica/metabolismo , Femenino , Humanos , Fenómenos del Sistema Inmunológico/efectos de los fármacos , Peroxidación de Lípido/efectos de los fármacos , Luminol/administración & dosificación , Luminol/uso terapéutico , Masculino , Persona de Mediana Edad , Pielonefritis/sangre , Pielonefritis/complicaciones , Pielonefritis/inmunología , Pielonefritis/terapia , Resultado del TratamientoRESUMEN
The peculiarities of cytokines as compounds of immunogenesis are shown in the patients having acute (A) and chronic (Ch) pyelonephritis (PN). The combination of antibacterial therapy with Nukleinat and Galavit promotes the positive changes of cytokin-producing ability of immunocompetent cells and decrease in the level of proinflammation cytokines in blood and urine, secretory leucocyte protease inhibitor (SLPI) in urine. In children with PN and adult patients with diagnostically elevated titres of antibodies (IgG) to Herpes simplex virus, Cytomegalovirus are shown the positive effects of Kanephron® H and Proteflazidum, accordingly. Clinico-immunological effects of immunomodulators testify to the expediency of this usage in complex therapy with the aim to modulate the cytokine link of immunity for improvement of the effective treatment in APN and the protection against aggravation of kidney functioning in ChPN.
Asunto(s)
Infecciones Bacterianas/tratamiento farmacológico , Infecciones por Citomegalovirus/tratamiento farmacológico , Herpes Simple/tratamiento farmacológico , Factores Inmunológicos/uso terapéutico , Inductores de Interferón/uso terapéutico , Pielonefritis/tratamiento farmacológico , Enfermedad Aguda , Adulto , Antibacterianos/uso terapéutico , Antioxidantes/uso terapéutico , Infecciones Bacterianas/complicaciones , Infecciones Bacterianas/inmunología , Infecciones Bacterianas/microbiología , Niño , Enfermedad Crónica , Citocinas/genética , Citocinas/inmunología , Infecciones por Citomegalovirus/complicaciones , Infecciones por Citomegalovirus/inmunología , Infecciones por Citomegalovirus/virología , Femenino , Expresión Génica , Herpes Simple/complicaciones , Herpes Simple/inmunología , Herpes Simple/virología , Humanos , Riñón/efectos de los fármacos , Riñón/inmunología , Riñón/patología , Luminol/análogos & derivados , Luminol/uso terapéutico , Masculino , Persona de Mediana Edad , Ácidos Nucleicos/uso terapéutico , Preparaciones de Plantas/uso terapéutico , Pielonefritis/complicaciones , Pielonefritis/inmunología , Pielonefritis/microbiología , Inhibidor Secretorio de Peptidasas Leucocitarias/genética , Inhibidor Secretorio de Peptidasas Leucocitarias/inmunologíaRESUMEN
Recent studies showed prominent antimicrobial activity of various plant extracts on certain pathogenic microorganisms, therefore we prepared crude aqueous extracts of black pepper, ginger and thyme and carried out an in vitro study by measuring antimicrobial activity of these extracts using the agar well diffusion method. An in vivo study was carried out on 50 adult healthy male albino rats which were divided into 5 groups, 10 rats each. Group 1: negative control group which received saline solution intragastrically daily; Group 2: Positive control group, injected with mixed bacterial suspension of S.aureus and E.coli as a model of pyelonephritis, then received saline solution intragastrically daily; Group 3: injected with the same dose of mixed bacterial suspension, then received 100 mg/kg/day black pepper extract intragastrically; Group 4: injected with mixed bacterial suspension then received 500 mg/kg/day ginger extract intragastrically. Group 5: injected with mixed bacterial suspension then received 500 mg/kg/day thyme extract intragastrically. All groups were sacrificed after either 1 or 4 weeks. Serum and blood samples were collected for lysozyme activity estimation using agarose lysoplate, measurement of nitric oxide production, and lymphocyte transformation test as well as for counting both total and differential leukocytes and erythrocytes. Kidney samples were tested histopathologically. Both in vivo and in vitro results confirm the efficacy of these extracts as natural antimicrobials and suggest the possibility of using them in treatment procedures.
Asunto(s)
Antiinfecciosos/farmacología , Piper nigrum , Extractos Vegetales/farmacología , Pielonefritis/tratamiento farmacológico , Thymus (Planta) , Zingiber officinale , Enfermedad Aguda , Animales , Modelos Animales de Enfermedad , Activación de Linfocitos/efectos de los fármacos , Masculino , Muramidasa/sangre , Óxido Nítrico/biosíntesis , Fitoterapia , Extractos Vegetales/uso terapéutico , Pielonefritis/inmunología , RatasRESUMEN
The present study included 70 patients (mean age 36.0 +- 1.5 years) presenting with chronic pyelonephritis. Thirty of them were given magnetolaserotherapy in addition to the conventional treatment. Their immune status as well as the levels of proinflammatory cytokines (interleukin-1 bets, interleukin-6, and tumour necrosis factor-alpha) were determined. It is shown that the combined treatment (basal therapy and magnetolaserotherapy) caused a significant decrease in the duration of the main clinical manifestations of the disease and normalization of the immune system and cytokine production.
Asunto(s)
Terapia por Láser/métodos , Magnetoterapia/métodos , Pielonefritis/terapia , Adulto , Enfermedad Crónica , Citocinas/inmunología , Femenino , Humanos , Masculino , Pielonefritis/inmunologíaRESUMEN
Of 59 patients with chronic calculous pyelonephritis (CCP) taking preparation therapy for extracorporeal lithotripsy, 29 patients received combined basic treatment (antibacterial drugs, phytotherapy, physiotherapy) plus polyoxidonium (a course of 10 intramuscular 6 mg injections each other day). The rest 30 patients (controls) received basic therapy alone. Polyoxidonium efficacy was assessed by the results of clinical, device and immunological investigations, content of the main phospholipid fractions and cholesterol in red cell membranes. The results of the tests show that polyoxidonium has anti-inflammatory and immunomodulating effects, exhibits activity of peroxidation processes, contributes to normalization of a lipid phase of cell membranes and can be recommended as a component of combined treatment of patients with chronic pyelonephritis and urolithiasis.
Asunto(s)
Antiinflamatorios/administración & dosificación , Factores Inmunológicos/administración & dosificación , Cálculos Renales/inmunología , Cálculos Renales/terapia , Piperazinas/administración & dosificación , Polímeros/administración & dosificación , Pielonefritis/inmunología , Pielonefritis/terapia , Antibacterianos/administración & dosificación , Enfermedad Crónica , Evaluación de Medicamentos , Femenino , Humanos , Inyecciones Intramusculares , Litotricia , Masculino , Lípidos de la Membrana/inmunología , FitoterapiaRESUMEN
Investigations performed by the authors proved pathogenetic validity of interference therapy of chronic pyelonephritis in children. A course of interference currents has a positive effect on partial renal functions, excretion of beta2-microglobulin, urodynamics of the lower urinary tract, humoral and cell links of immunity, lipid peroxidation in red cell membranes. Advantages of interference currents impact on the reflex-segmentary zone and urinary bladder projection area in children with concomitant neurogenic dysfunction of the urinary bladder are shown.
Asunto(s)
Terapia por Estimulación Eléctrica , Pielonefritis/terapia , Adolescente , Niño , Preescolar , Enfermedad Crónica , Terapia Combinada , Eritrocitos/inmunología , Eritrocitos/fisiología , Humanos , Inmunoglobulinas/inmunología , Peróxidos Lipídicos/metabolismo , Recuento de Linfocitos , Pielonefritis/tratamiento farmacológico , Pielonefritis/inmunología , Pielonefritis/fisiopatología , Linfocitos T/citología , Linfocitos T/inmunología , Resultado del Tratamiento , Vejiga Urinaria/fisiopatología , Micción/fisiologíaRESUMEN
Revealed in the wake of balneotherapy at the Truskavets health-resort were two opposite types of changes--those in the level of uriacidemia and in the content of theophylline-sensitive lymphocytes that are probably caused by central and peripheral effects of uric acid. In this setting, parameters characterizing the phagocytic link of immunity and unspecific defense are noted to change unidirectionally, in other words, they remain unaffected by changes in uriacidemia.
Asunto(s)
Balneología , Hiperuricemia/inmunología , Fagocitosis/inmunología , Ácido Úrico , Colonias de Salud , Humanos , Hiperuricemia/sangre , Hiperuricemia/etiología , Hiperuricemia/terapia , Inmunidad Celular/inmunología , Aguas Minerales , Pielonefritis/complicaciones , Pielonefritis/inmunología , Linfocitos T/inmunología , Ucrania , Ácido Úrico/sangre , Ácido Úrico/metabolismo , Cálculos Urinarios/complicaciones , Cálculos Urinarios/inmunologíaRESUMEN
OBJECTIVE: To investigate the relationship between chronic pyelonephritis (CPN) and immune function, and the therapeutic mechanism of Yishenkang granule (YSKG). METHODS: One hundred and twenty patients of CPN were divided into 3 groups randomly, the YSKG group (treated with YSKG), the control A group (treated with Sanjin tablet) and the control B group (treated with western medicine). Serum levels of immunoglobulin (Ig), complement 3 (C3), interleukin-2 (IL-2), peripheral T-lymphocyte subsets, and urinary secretory immunoglobulin A (sIgA) were determined before and after treatment with monoclonal antibody assay, agar diffusion method, radioimmunoassay (RIA), and radioimmuno-equilibrium method, and compared with normal control. RESULTS: There were disorders of T-lymphocyte subsets in CPN, lowering of serum Ig, C3 and urinary sIgA, and increase of blood IL-2 (P < 0.05 or P < 0.01). These abnormalities could be normalized after YSKG treatment. CONCLUSION: Functional disorders of cellular and humoral immunity exist in CPN patients of chronic stage, YSKG could correct the immune functional disorder, control the recurrence of CPN effectively and alleviate the immunopathological damage.
Asunto(s)
Medicamentos Herbarios Chinos/uso terapéutico , Interleucina-2/sangre , Pielonefritis/tratamiento farmacológico , Subgrupos de Linfocitos T/efectos de los fármacos , Adulto , Enfermedad Crónica , Complemento C3/metabolismo , Femenino , Humanos , Inmunoglobulina G/sangre , Masculino , Persona de Mediana Edad , Pielonefritis/inmunología , Pielonefritis/prevención & controlRESUMEN
The employing of Velikobagachanskaia mineral water internally by drinking and externally by taking a bath with it adds to the effectiveness of treatment of chronic pyelonephritis, modulates parameters associated with immune homeostasis, reduces the number of carriers of antibodies to M. hominis, facilitates prolongation of clinical and laboratory remission.
Asunto(s)
Baños , Aguas Minerales/uso terapéutico , Pielonefritis/rehabilitación , Adolescente , Adulto , Anciano , Anticuerpos Antibacterianos/sangre , Enfermedad Crónica , Colonias de Salud , Humanos , Inmunoglobulinas/sangre , Persona de Mediana Edad , Mycoplasma hominis/inmunología , Pielonefritis/inmunología , Factores de Tiempo , UcraniaAsunto(s)
Aguas Minerales/administración & dosificación , Pielonefritis/rehabilitación , Adolescente , Formación de Anticuerpos , Niño , Enfermedad Crónica , Ritmo Circadiano , Colonias de Salud , Humanos , Inmunidad Celular , Inmunidad Innata , Fagocitosis , Pielonefritis/inmunología , Pielonefritis/metabolismoRESUMEN
The authors present a technique of treating chronic calculous pyelonephritis with laser radiation and sinusoidal modulated currents which promotes a complete elimination of the calculus fragments in 100, 70 and 50% of the patients in the stone size 0.2-0.5 cm, 0.5-0.7 cm and > 7 cm, respectively. This combined therapy had also antiinflammatory, and immunity-stimulating effects.
Asunto(s)
Terapia por Estimulación Eléctrica/métodos , Terapia por Láser , Pielonefritis/rehabilitación , Cálculos Urinarios/rehabilitación , Formación de Anticuerpos/efectos de la radiación , Enfermedad Crónica , Terapia Combinada , Electrólitos/sangre , Electrólitos/efectos de la radiación , Humanos , Inmunidad Celular/efectos de la radiación , Inmunoglobulinas/sangre , Inmunoglobulinas/efectos de la radiación , Pielonefritis/sangre , Pielonefritis/inmunología , Cálculos Urinarios/sangre , Cálculos Urinarios/inmunologíaRESUMEN
51 patients with urolithiasis complicated by pyelonephritis in the active phase of inflammation were studied for the condition of local immunity by determining the urine content of secretory immunoglobulin A (SIgA) under conditions of combined treatment with making use of phlogenzyme, a drug of II-generation systemic enzymotherapy (SE). Recordable in this patients population was a marked increase in the urine level of SIgA. Incorporation in a combined treatment of phlogenzyme results in normalizing the status of the urinary system local immunity. Evidence has been obtained on the lack of parallelism in the dynamics between the serum IgA content and urine concentration of SIgA, which fact suggests independence of local immunity. Our theory is that an appreciable increase in the urine level of SIgA in patients with urolithiasis concurrent with pyelonephritis may have an important part to play in the genesis of nephrolithiasis.
Asunto(s)
Cálculos Urinarios/inmunología , Sistema Urinario/inmunología , Adulto , Anciano , Bromelaínas/uso terapéutico , Terapia Combinada , Combinación de Medicamentos , Femenino , Humanos , Inmunidad , Inmunoglobulina A Secretora/orina , Litotricia , Masculino , Persona de Mediana Edad , Pielonefritis/etiología , Pielonefritis/inmunología , Pielonefritis/terapia , Recurrencia , Rutina/análogos & derivados , Rutina/uso terapéutico , Tripsina/uso terapéutico , Cálculos Urinarios/complicaciones , Cálculos Urinarios/terapia , Sistema Urinario/cirugíaAsunto(s)
Bicarbonatos/uso terapéutico , Compuestos de Calcio/uso terapéutico , Colonias de Salud , Compuestos de Magnesio/uso terapéutico , Aguas Minerales/uso terapéutico , Pielonefritis/inmunología , Pielonefritis/rehabilitación , Enfermedad Crónica , Humanos , Inmunidad Celular , Inducción de Remisión , SiberiaRESUMEN
The exposure of the lumbar region to interference currents in combination with radon baths conducted in 37 patients with chronic pyelonephritis inhibited activity of the inflammation, hypercalciuria and hyperoxaluria, improved function of the kidneys. The same clinical effects were achieved in 32 patients who had received interference therapy in combination with intake of radon water.
Asunto(s)
Baños/métodos , Terapia por Estimulación Eléctrica/métodos , Pielonefritis/rehabilitación , Radón/uso terapéutico , Adolescente , Adulto , Anciano , Formación de Anticuerpos , Enfermedad Crónica , Terapia Combinada , Humanos , Inmunidad Celular , Riñón/fisiopatología , Persona de Mediana Edad , Pielonefritis/inmunología , Pielonefritis/fisiopatología , Pielonefritis/orina , Inducción de RemisiónRESUMEN
30 patients were exposed to interference currents (IC) alone (group 1) versus 34 patients treated with IC plus iodobromine baths (group 2). After IC action on the region of the kidney projection patients of group 1 experienced positive changes in cellular and humoral immunity providing an antiinflammatory effect. In group 2, the improvement was seen in renal and urinary functions, 24-h urinary excretion of oxalates and calcium diminished. Interference current proved beneficial in chronic pyelonephritis patients with latent inflammation. The combined therapy was effective in patients at high risk of lithogenesis.
Asunto(s)
Baños/métodos , Bromuros/uso terapéutico , Terapia por Estimulación Eléctrica/métodos , Yoduros/uso terapéutico , Pielonefritis/rehabilitación , Adolescente , Adulto , Anciano , Formación de Anticuerpos , Enfermedad Crónica , Terapia Combinada , Estudios de Evaluación como Asunto , Humanos , Inmunidad Celular , Persona de Mediana Edad , Pielonefritis/inmunología , Pielonefritis/metabolismoRESUMEN
The authors have developed combined therapy of secondary chronic pyelonephritis with immune and urodynamic dysfunctions. The treatment includes physical factors: decimeter microwaves, gangleron-electrophoresis with sinusoidal modulated currents, sodium chloride baths, oral mineral water Moskovskaia. The adjuvant use of the above physical factors proved advantageous.