An outbreak of trichomonosis in European greenfinches Chloris chloris and European goldfinches Carduelis carduelis wintering in Northern France.

Avian trichomonosis is a common and widespread disease, traditionally affecting columbids and raptors, and recently emerging among finch populations mainly in Europe. Across Europe, finch trichomonosis is caused by a single clonal strain of Trichomonas gallinae and negatively impacts finch populations. Here, we report an outbreak of finch trichomonosis in the wintering populations of Chloris chloris (European greenfinch) and Carduelis carduelis (European goldfinch) from the Boulonnais, in northern France. The outbreak was detected and monitored by bird ringers during their wintering bird ringing protocols. A total of 105 records from 12 sites were collected during the first quarter of 2017, with 46 and 59 concerning dead and diseased birds, respectively. Fourteen carcasses from two locations were necropsied and screened for multiple pathogens; the only causative agent identified was T. gallinae. Genetic characterization was performed by four markers (small subunit ribosomal RNA, hydrogenosomal iron-hydrogenase, and RNA polymerase II subunit 1 genes, and the internal transcribed spacers (ITS) region) and confirmed the T. gallinae strain to be A1, which affects the finch populations of Europe. This was also confirmed by an ITS-based phylogenetic analysis which further illustrated the diversity of the Trichomonas infecting birds. Preliminary data on the survival and dispersion of infected birds were obtained from ring-returns of diseased individuals. The anthropogenic spread of diseases through bird feeding practices is highlighted and some suggestions to prevent pathogen transmission via backyard supplementary feeders for garden birds are given.

Effects of carotenoids, immune activation and immune suppression on the intensity of chronic coccidiosis in greenfinches.

Allocation trade-offs of carotenoids between their use in the immune system and production of integumentary colouration have been suggested as a proximate mechanism maintaining honesty of signal traits. We tested how dietary carotenoid supplementation, immune activation and immune suppression affect intensity of coccidian infection in captive greenfinches Carduelis chloris, a passerine with carotenoid-based plumage. Immune activation with phytohaemagglutinin (PHA) decreased body mass among birds not supplemented with lutein, while among the carotenoid-fed birds, PHA had no effect on mass dynamics. Immune suppression with dexamethasone (DEX) induced loss of body mass and reduced the swelling response to PHA. DEX and PHA increased the concentration of circulating heterophils. Lutein supplementation increased plasma carotenoid levels but had no effect on the swelling response induced by PHA. PHA and DEX treatments did not affect plasma carotenoids. Immune stimulation by PHA suppressed the infection, but only among carotenoid-supplemented birds. Priming of the immune system can thus aid in suppressing chronic infection but only when sufficient amount of carotenoids is available. Our experiment shows the importance of carotenoids in immune response, but also the complicated nature of this impact, which could be the reason for inconsistent results in studies investigating the immunomodulatory effects of carotenoids. The findings about involvement of carotenoids in modulation of an immune response against coccidiosis suggest that carotenoid-based ornaments may honestly signal individuals' ability to manage chronic infections.

A multifactorial test of the effects of carotenoid access, food intake and parasite load on the production of ornamental feathers and bill coloration in American goldfinches.

It has been well established that carotenoid and melanin pigmentation are often condition-dependent traits in vertebrates. Expression of carotenoid coloration in birds has been shown to reflect pigment intake, food access and parasite load; however, the relative importance of and the potential interactions among these factors have not been previously considered. Moreover, carotenoid and melanin pigmentation have been proposed to signal fundamentally different aspects of individual condition but few data exist to test this idea. We simultaneously manipulated three environmental conditions under which American goldfinches (Cardeulis tristis) grew colorful feathers and developed carotenoid pigmentation of their bills. Male goldfinches were held with either high or low carotenoid supplementation, pulsed or continuous antimicrobial drug treatment, or restricted or unlimited access to food. Carotenoid supplementation had an overriding effect on yellow feather coloration. Males given more lutein and zeaxanthin grew yellow feathers with hue shifted toward orange and with higher yellow chroma than males supplemented with fewer carotenoids. Parasites and food access did not significantly affect yellow feather coloration, and there were only minor interaction effects for the three treatments. By contrast, bill coloration was significantly affected by all three treatments. Carotenoid supplementation had a significant effect on yellow chroma of bills, drug treatment and food access both had a significant effect on bill hue, and food access had a significant effect on the yellow brightness of bills. Neither the size nor blackness of the black caps of male goldfinches was affected by any treatment. These results indicate that pigment intake, food access and parasite load can have complex and variable effects on color displays, and that feather and bill coloration signal different aspects of male condition.