RESUMEN
Medicinal plants are playing an imperative role in the therapy for treating various chronic ailments including arthritis. The present study was focused on finding in-vitro and in-vivo anti-arthritic potential of P. braunii roots. In vitro protein denaturation, membrane stabilization and anti-trypsinase assays were carried out to demonstrate anti-arthritic activity of the extracts. Furthermore, the extracts exerting promising in vitro anti-arthritic potential were tested orally at 150, 300 and 600mg/kg/day against formaldehyde induced arthritis in Wistar rats. The methanolic, aqueous and ethyl acetate extracts of the plant revealed noteworthy in vitro anti-arthritic activities while mitigating formaldehyde induced paw edema in dose dependent manner. Methanolic and aqueous extracts showed the highest inhibition (p<0.05) of paw edema, arthritic indices, reduced elevated level of platelets and leukocytes while increasing hemoglobin and body weight of arthritic rats. Anti-arthritic activity of the plant extracts may be due to inhibition of protein denaturation and lysosomal membrane stabilization. The plant exhibited good anti-arthritic potential.
Asunto(s)
Artritis Experimental/tratamiento farmacológico , Extractos Vegetales/farmacología , Plantas Medicinales/química , Polystichum/química , Albúminas/química , Albúminas/efectos de los fármacos , Animales , Artritis Experimental/inducido químicamente , Evaluación Preclínica de Medicamentos , Membrana Eritrocítica/efectos de los fármacos , Femenino , Formaldehído/toxicidad , Humanos , Masculino , Medicina Tradicional de Asia Oriental , Pakistán , Extractos Vegetales/química , Raíces de Plantas/química , Desnaturalización Proteica/efectos de los fármacos , Ratas Wistar , Albúmina Sérica Bovina/efectos de los fármacosRESUMEN
The functionality of spring mountain herbs, which were collected in the Kajigamori mountain area of Shikoku area in Japan, was investigated in the course of our studies for utilizing local plant resources. The radical scavenging activity of the extracts from seventeen herbs was measured. Among these herbs, two extracts from Polystichym ovato-paleaceum (Japanese name: Tsuyanashiinode) and Sambucus racemosa subsp. sieboldiana (Japanese name: Niwatoko) showed potent DPPH radical scavenging activity. The material evidence for the potent activity of the extracts was studied by a combination of our developed method for detecting antiradical compounds, LC-MS/MS, and enzymatic hydrolysis.