RESUMEN
Asthma is a high-incidence disease in the world. Oxysophocarpine (OSC), a quinolizidine alkaloid displays various pharmacological functions including anti-inflammation, neuroprotective, anti-virus and antioxidant. Here, we established mice and cell asthmatic model to explore the effects of OSC for asthma treatment. Mice were sensitized and challenged with ovalbumin (OVA) and treated with OSC before challenge. Enzyme-linked immuno sorbent assay (ELISA), hematoxylin and eosin (H&E), periodic acid-schiff (PAS), tolonium chloride staining and immunohistochemical assay were performed. OSC treatment inhibited inflammatory cell infiltration and mucus secretion in the airway, reduced IgE level in mouse serum and decreased IL-4, IL-5 production in bronchoalveolar lavage fluid (BALF). OSC also reduced the spleen index to regulate immune function. Meanwhile, NCI-H292 cells were induced by lipopolysaccharide (LPS) to simulate airway epithelial injury. OSC pretreatment decreased the IL-6 and IL-8 cytokine levels, mucin 5 AC expression, and mucin 5 AC mRNA level in the cell model. Further, OSC suppressed the phosphorylation of c-Jun N-terminal kinase (JNK), and activator protein 1 (AP-1, Fos and Jun). These findings revealed that OSC alleviated bronchial asthma associated with JNK/AP-1 signaling pathway.
Asunto(s)
Alcaloides , Asma , Quinolizidinas , Alcaloides/metabolismo , Alcaloides/farmacología , Animales , Antioxidantes/farmacología , Asma/tratamiento farmacológico , Citocinas/metabolismo , Modelos Animales de Enfermedad , Eosina Amarillenta-(YS)/metabolismo , Eosina Amarillenta-(YS)/farmacología , Eosina Amarillenta-(YS)/uso terapéutico , Hematoxilina/metabolismo , Hematoxilina/farmacología , Hematoxilina/uso terapéutico , Inmunoglobulina E , Interleucina-4/metabolismo , Interleucina-4/farmacología , Interleucina-4/uso terapéutico , Interleucina-5/metabolismo , Interleucina-5/farmacología , Interleucina-5/uso terapéutico , Interleucina-6/metabolismo , Interleucina-8/metabolismo , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Lipopolisacáridos/farmacología , Pulmón , Ratones , Ratones Endogámicos BALB C , Estructura Molecular , Mucinas/metabolismo , Mucinas/farmacología , Mucinas/uso terapéutico , Moco/metabolismo , Ovalbúmina/metabolismo , Ácido Peryódico/metabolismo , Ácido Peryódico/farmacología , Ácido Peryódico/uso terapéutico , Quinolizidinas/farmacología , ARN Mensajero/metabolismo , Cloruro de Tolonio/metabolismo , Cloruro de Tolonio/farmacología , Cloruro de Tolonio/uso terapéutico , Factor de Transcripción AP-1/metabolismo , Factor de Transcripción AP-1/farmacología , Factor de Transcripción AP-1/uso terapéuticoRESUMEN
Fusarium oxysporum is an aggressive phytopathogen that affects various plant species, resulting in extensive local and global economic losses. Therefore, the search for competent alternatives is a constant pursuit. Quinolizidine alkaloids (QA) are naturally occurring compounds with diverse biological activities. The structural diversity of quinolizidines is mainly contributed by species of the family Fabaceae, particularly the genus Lupinus. This quinolizidine-based chemo diversity can be explored to find antifungals and even mixtures to address concomitant effects on F. oxysporum. Thus, the antifungal activity of quinolizidine-rich extracts (QREs) from the leaves of eight greenhouse-propagated Lupinus species was evaluated to outline promising QA mixtures against F. oxysporum. Thirteen main compounds were identified and quantified using an external standard. Quantitative analysis revealed different contents per quinolizidine depending on the Lupinus plant, ranging from 0.003 to 32.8 mg/g fresh leaves. Bioautography showed that all extracts were active at the maximum concentration (5 µg/µL). They also exhibited >50% mycelium growth inhibition. All QREs were fungistatic except for the fungicidal QRE of L. polyphyllus Lindl. Angustifoline, matrine, 13α-hydroxylupanine, and 17-oxolupanine were ranked to act jointly against the phytopathogen. Our findings constitute reference information to better understand the antifungal activity of naturally afforded QA mixtures from these globally important plants.
Asunto(s)
Antifúngicos/farmacología , Lupinus/química , Extractos Vegetales/farmacología , Quinolizidinas/farmacología , Antifúngicos/química , Cromatografía de Gases y Espectrometría de Masas , Efecto Invernadero , Lupinus/crecimiento & desarrollo , Pruebas de Sensibilidad Microbiana , Fitoquímicos/química , Fitoquímicos/farmacología , Extractos Vegetales/química , Quinolizidinas/químicaRESUMEN
Type 1 diabetes (T1D) is a metabolic dysfunction characterized by the selective destruction of islet ß-cells, with oxidative stress playing an essential role in the manifestation of this disease state. Aloperine (ALO) represents the main active alkaloid extracted from the traditional Chinese herbal Sophora alopecuroides L. and features outstanding antioxidative properties. In this study, T1D was induced by a single high dose streptozotocin (STZ, 150 mg/kg, intraperitoneal) in mice. Diabetic animals were intragastrically administered ALO at a dose of 50 mg/kg/day. Notably, treatment of ALO (50 mg/kg/day) for seven consecutive days could observably reverse the onset of diabetes induced by STZ accompanied by weight gain, lower blood glucose levels, and relief of ß-cells damage. Our in vitro study further demonstrated that ALO protected ß-cells from STZ/hydrogen peroxide-induced oxidative damage as manifested by increased expression of MnSOD and CAT. Furthermore, a network pharmacology study revealed that NOS1 represented the main target of ALO. Mechanistic studies subsequently showed that treatment of ALO increased the expression of NOS1, whereas NOS2 was decreased. Moreover, a docking study carried out suggested that ALO could fit into the binding pocket of human NOS1 and molecular dynamics simulation further validated this docking event. Collectively, the administration of ALO prior to diabetes could be a viable approach to the prevention of ß-cell injury. This study may offer a novel potential herbal medicine against T1D and may further help improve the understanding of the underlying molecular mechanisms of ALO-mediated protection against oxidative stress.
Asunto(s)
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 1 , Células Secretoras de Insulina , Óxido Nítrico Sintasa de Tipo I , Quinolizidinas , Animales , Glucemia/metabolismo , Citoprotección , Diabetes Mellitus Experimental/tratamiento farmacológico , Diabetes Mellitus Tipo 1/tratamiento farmacológico , Células Secretoras de Insulina/efectos de los fármacos , Células Secretoras de Insulina/patología , Ratones , Óxido Nítrico Sintasa de Tipo I/metabolismo , Estrés Oxidativo , Piperidinas/farmacología , Quinolizidinas/administración & dosificación , Quinolizidinas/farmacología , Quinolizidinas/uso terapéutico , EstreptozocinaRESUMEN
Cervical cancer (CC) is recognized as the most common neoplasm in the female reproductive system worldwide. The lack of chemotherapeutic agents with outstanding effectiveness and safety severely compromises the anti-cipated prognosis of patients. Aloperine (ALO) is a natural quinolizidine alkaloid with marked anti-cancer effects on multiple malignancies as well as favorable activity in relieving inflammation, allergies and infection. However, its therapeutic efficacy and underlying mechanism in CC are still unclear. In the current study, MTT assay was employed to evaluate the viability of HeLa cells exposed to ALO to preliminarily estimate the effectiveness of ALO in CC. Then, the effects of ALO on the proliferation and apoptosis of HeLa cells were further investigated by plate colony formation and flow cytometry, respectively, while the migration and invasion of ALO-treated HeLa cells were evaluated using Transwell assay. Moreover, nude mice were subcutaneously inoculated with HeLa cells to demonstrate the anti-CC properties of ALO in vivo. The molecular mechanisms underlying these effects of ALO were evaluated by Western blot and immunohistochemical analysis. This study experimentally demonstrated that ALO inhibited the proliferation of HeLa cells via G2 phase cell cycle arrest. Simultaneously, ALO promoted an increase in the percentage of apoptotic HeLa cells by increasing the Bax/Bcl-2 ratio. Additionally, the migration and invasion of HeLa cells were attenuated by ALO treatment, which was considered to result from inhibition of epithelial-to-mesenchymal transition. For molecular mechanisms, the expression and activation of the IL-6-JAK1-STAT3 feedback loop were markedly suppressed by ALO treatment. This study indicated that ALO markedly suppresses the proliferation, migration and invasion and enhances the apoptosis of HeLa cells. In addition, these prominent anti-CC properties of ALO are associated with repression of the IL-6-JAK1-STAT3 feedback loop.
Asunto(s)
Quinolizidinas/farmacología , Neoplasias del Cuello Uterino , Animales , Apoptosis , Línea Celular Tumoral , Movimiento Celular , Proliferación Celular , Retroalimentación , Femenino , Células HeLa , Humanos , Interleucina-6/genética , Janus Quinasa 1/genética , Ratones , Ratones Desnudos , Factor de Transcripción STAT3/genética , Transducción de Señal , Neoplasias del Cuello Uterino/tratamiento farmacológicoRESUMEN
Presently, postmenopausal osteoporosis mainly caused by excessive activation of in vivo osteoclasts has become a global public health burden. Natural compounds have gradually become the potential drugs for the treatment of postmenopausal osteoporosis. Aloperine is a new alkaloid extracted from the leaves and seeds of sophora bean. The current studies have proved that aloperine has many biological activities, including anti-inflammatory, antiviral and anticancer activities. This study shows that aloperine can inhibit activity and formation of osteoclast mediated by RANKL in a dose-dependent manner without affecting the activity of bone marrow macrophages (BMM). In addition, it is found that aloperine can inhibit the expression of osteoclast specific marker genes, including nuclear factor of activated T cells cytoplasmic 1 (NFATc1), tartrate resistant acid phosphatase (TRAcP), matrix metallopeptidase 9 (MMP9), cathepsin K (Ctsk), V-ATPase d2 and calcitonin receptor. The in vitro experiment of aloperine proved that aloperine can inhibit the degradation of IκBα and the phosphorylation of P65, ERK and JNK. Additionally, aloperine improves bone loss in ovariectomized (OVX) mice by inhibiting osteoclast activity. This project proved that aloperine can affect the formation of osteoclasts by inhibiting RANKL signaling channel, and it is indicated that aloperine has the potential to be developed as a new drug for the prevention and treatment of postmenopausal osteoporosis.
Asunto(s)
Osteogénesis/efectos de los fármacos , Osteoporosis Posmenopáusica/tratamiento farmacológico , Quinolizidinas/farmacología , Animales , Modelos Animales de Enfermedad , Evaluación Preclínica de Medicamentos , Femenino , Fémur/efectos de los fármacos , Fémur/patología , Humanos , Quinasas Janus/antagonistas & inhibidores , Quinasas Janus/metabolismo , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Ratones , FN-kappa B/antagonistas & inhibidores , FN-kappa B/metabolismo , Osteoclastos/efectos de los fármacos , Osteoporosis Posmenopáusica/diagnóstico , Osteoporosis Posmenopáusica/etiología , Osteoporosis Posmenopáusica/patología , Ovariectomía , Quinolizidinas/uso terapéutico , Ligando RANK/antagonistas & inhibidores , Ligando RANK/metabolismo , Microtomografía por Rayos XRESUMEN
The discovery of novel, effective, and botanical pesticides is one of the main strategies for modern plant protection and insect pest control. During the search for novel botanical pesticides from natural sources, the seeds of Sophora tonkinensis were systematically investigated to obtain 11 new matrine-type alkaloids (1-11), including one novel matrine-type alkaloid featuring an unprecedented 5/6/6/6 tetracyclic skeleton (1), along with 16 known compounds (12-27). Their structures were elucidated by comprehensive spectroscopic data analysis (IR, UV, NMR, and HRESIMS), ECD calculations, and single-crystal X-ray diffraction. The anti-tobacco mosaic virus (TMV) activity and insecticidal activities against Aphis fabae and Tetranychus urticae of the compounds were also respectively screened using the half-leaf method and spray method. Biological tests indicated that compounds 2, 4, 6, and 26 displayed significant anti-TMV biological activities compared with the positive control ningnanmycin. Compounds 7, 17, and 26 presented moderate activities against A. fabae with LC50 values of 38.29, 18.63, and 23.74 mg/L, respectively. Moreover, compounds 13 and 26 exhibited weak activities against T. urticae.
Asunto(s)
Alcaloides/farmacología , Antivirales/farmacología , Insecticidas/farmacología , Extractos Vegetales/farmacología , Quinolizidinas/farmacología , Sophora/química , Alcaloides/química , Animales , Antivirales/química , Insectos/efectos de los fármacos , Insectos/crecimiento & desarrollo , Insecticidas/química , Extractos Vegetales/química , Quinolizidinas/química , Semillas/química , Virus del Mosaico del Tabaco/efectos de los fármacos , Virus del Mosaico del Tabaco/crecimiento & desarrolloRESUMEN
Two new quinolizidine-based alkaloids (2 and 12), along with ten known ones (1, 3-11) were isolated from the roots of S. tonkinensis. Their structures were determined by spectroscopic data (including NMR, MS, IR, and UV), X-ray single crystal diffraction, electronic circular dichroism analyses (ECD), and comparing with related literatures. Compounds 1, 3-12 at non-toxic concentrations exhibited potent anti-inflammatory activities according to in vitro and in vivo anti-inflammatory tests. Among them, (-)-anagyrine (4), sophocarpine (8), 14ß-hydroxymatrine (10), and 7ß-sophoramine (12) showed more potent in vitro anti-inflammatory activities, and 5α,14ß-dihydroxymatrine (2), (-)-anagyrine (4), sophocarpine (8), and 5α-hydroxymatrine (9) exhibited better in vivo anti-inflammatory effects.
Asunto(s)
Alcaloides/farmacología , Antiinflamatorios/farmacología , Quinolizidinas/farmacología , Sophora/química , Alcaloides/aislamiento & purificación , Animales , Animales Modificados Genéticamente , Antiinflamatorios/aislamiento & purificación , Azocinas , China , Ratones , Estructura Molecular , Fitoquímicos/aislamiento & purificación , Fitoquímicos/farmacología , Raíces de Plantas/química , Quinolizidinas/aislamiento & purificación , Quinolizinas , Células RAW 264.7 , Pez CebraRESUMEN
Quinolizidine alkaloids, a main form of alkaloids found in the genus Sophora, have been shown to have many pharmacological effects. This review aims to summarize the photochemical reports and biological activities of quinolizidine alkaloids in Sophora. The collected information suggested that a total of 99 quinolizidine alkaloids were isolated and detected from different parts of Sophora plants, represented by lupinine-type, cytisine-type, sparteine-type, and matrine-type. However, quality control needs to be monitored because it could provide basic information for the reasonable and efficient use of quinolizidine alkaloids as medicines and raw materials. The nonmedicinal parts may be promising to be used as a source of quinolizidine alkaloid raw materials and to reduce the waste of resources and environmental pollution. In addition, the diversity of chemical compounds based on the alkaloid scaffold to make a biological compound library needs to be extended, which may reduce toxicity and find new bioactivities of quinolizidine alkaloids. The bioactivities most reported are in the fields of antitumor activity along with the effects on the cardiovascular system. However, those studies rely on theoretical research, and novel drugs based on quinolizidine alkaloids are expected.
Asunto(s)
Alcaloides/farmacología , Extractos Vegetales/farmacología , Quinolizidinas/farmacología , Sophora/química , Alcaloides/aislamiento & purificación , Alcaloides/normas , Alcaloides/uso terapéutico , Analgésicos/aislamiento & purificación , Analgésicos/farmacología , Analgésicos/uso terapéutico , Antiinfecciosos/aislamiento & purificación , Antiinfecciosos/farmacología , Antiinfecciosos/uso terapéutico , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/farmacología , Antiinflamatorios/uso terapéutico , Antimetabolitos/aislamiento & purificación , Antimetabolitos/farmacología , Antimetabolitos/uso terapéutico , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Antineoplásicos/normas , Antineoplásicos/uso terapéutico , Fármacos Cardiovasculares/aislamiento & purificación , Fármacos Cardiovasculares/farmacología , Fármacos Cardiovasculares/uso terapéutico , Desarrollo de Medicamentos , Descubrimiento de Drogas , Humanos , Insecticidas , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/normas , Extractos Vegetales/uso terapéutico , Control de Calidad , Quinolizidinas/aislamiento & purificación , Quinolizidinas/normas , Quinolizidinas/uso terapéuticoRESUMEN
Quinolizidine alkaloids are the main bioactive components in Sophora alopecuroides L. This study reports a novel ultrasound-assisted enzymatic hydrolysis method for the extraction of these important alkaloids. Box-Behnken design, a widely used response surface methodology, was used to investigate the effects of process variables on ultrasound bath-assisted enzymatic hydrolysis (UAEH) extraction. Four independent variables, pH, extraction temperature (°C), extraction time (min) and solvent-to-material ratio (mL/g), were studied. For the extraction of sophocarpine, oxysophocarpine, oxymatrine, matrine, sophoramine, sophoridine and cytisine, the optimal UAEH condition was found to be a pH of 5, extraction temperature of 54 °C, extraction time of 60 min and solvent-to-material ratio of 112 mL/g. The experimental values obtained under optimal conditions were fairly consistent with the predicted values. UAEH extraction was then compared with reflux heating, enzymatic extraction and ultrasound-assisted extraction. Of these extraction methods, UAEH extraction under optimal conditions produced the highest yield for seven types of alkaloids. In addition, UAEH extraction resulted in lower ingredient degradation than reflux heating extraction.
Asunto(s)
Quinolizidinas/uso terapéutico , Semillas/química , Sophora/química , Ultrasonografía/métodos , Alcaloides , Hidrólisis , Quinolizidinas/farmacologíaRESUMEN
Alzheimer's disease (AD) is a progressive and neurodegenerative disorder and one of the current therapies involves strengthening the cholinergic tone in central synapses. Neuroprotective properties for nicotine have been described in AD, through its actions on nicotinic receptors and the further activation of the PI3K/Akt/Bcl-2 survival pathway. We have tested a quinolizidine alkaloid extract (TM0112) obtained from Teline monspessulanna (L.) K. Koch seeds to evaluate its action on nicotinic acetylcholine receptor (nAChR) in a neuronal AD model. Our data show that PC-12 cells pretreated with amyloid-ß (Aß) peptide for 24 h in presence of TM0112 modified Aß-reduction on cellular viability (Aß = 80 ± 3%; +TM0112 = 113 ± 4%, n = 15). In addition, this effect was blocked with atropine, MLA, and α-BTX (+TM0112+atropine = 87 ± 4%; +TM0112+MLA = 86 ± 4%; +TM0112+α-BTX = 92 ± 3%). Furthermore, similar protective effects were observed in rat cortical neurons (Aß = 63 ± 6%; +TM0112 = 114 ± 8%), but not in HEK293T cells (Aß = 61.4 ± 6.1%; +TM0112 = 62.8 ± 5.2) that do not express α7 nAChR. Moreover, the frequency of synaptic activity in the neuronal network (Aß = 51.6 ± 16.9%; +TM0112 = 210.8 ± 47.9%, n > 10), as well as the intracellular Ca2+ transients were recovered by TM0112 (Aß = 61.4 ± 6.9%; +TM0112 = 112.0 ± 5.7%; n = 3) in rat hippocampal neurons. TM0112 increased P-Akt, up to 250% with respect to control, and elevated Bcl-2/Bax percentage (Aß = 61.0 ± 8.2%; +TM0112 = 105.4 ± 19.5%, n = 4), suggesting a coupling between nAChR activation and an intracellular neuroprotective pathway. Our results suggest that TM0112 could be a new potential source for anti-AD drugs.
Asunto(s)
Alcaloides/farmacología , Enfermedad de Alzheimer/tratamiento farmacológico , Neuronas/efectos de los fármacos , Fármacos Neuroprotectores/farmacología , Quinolizidinas/farmacología , Receptores Nicotínicos/metabolismo , Enfermedad de Alzheimer/fisiopatología , Péptidos beta-Amiloides/toxicidad , Animales , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Corteza Cerebral/efectos de los fármacos , Corteza Cerebral/fisiopatología , Fabaceae , Células HEK293 , Humanos , Neuronas/fisiología , Células PC12 , Fragmentos de Péptidos/toxicidad , Fitoterapia , Extractos Vegetales/farmacología , Ratas , Ratas Sprague-Dawley , Semillas , Transmisión Sináptica/efectos de los fármacosRESUMEN
Four new biphenyl and biphenyl ether quinolizidine N-oxide alkaloids, 5- EPI-dihydrolyfoline N-oxide (1), decamine N-oxide (2), lagerstroemine N-oxide (3), and lagerine N-oxide (4), were isolated from the aerial parts of Lagerstroemia indica, and their structures were established by extensive spectroscopic studies. In addition, the inhibitory effects of isolated compounds on rat lens aldose reductase (RLAR) were examined.
Asunto(s)
Alcaloides/química , Compuestos de Bifenilo/química , Óxidos N-Cíclicos/química , Lagerstroemia/química , Quinolizidinas/química , Aldehído Reductasa/efectos de los fármacos , Alcaloides/aislamiento & purificación , Alcaloides/farmacología , Animales , Compuestos de Bifenilo/aislamiento & purificación , Compuestos de Bifenilo/farmacología , Fraccionamiento Químico , Óxidos N-Cíclicos/aislamiento & purificación , Óxidos N-Cíclicos/farmacología , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/aislamiento & purificación , Inhibidores Enzimáticos/farmacología , Éteres/química , Espectroscopía de Resonancia Magnética , Componentes Aéreos de las Plantas/química , Quinolizidinas/aislamiento & purificación , Quinolizidinas/farmacología , Ratas , Ratas Sprague-DawleyRESUMEN
The dry root of Sophora flavescens Ait. (SF) has long been used in a variety of Chinese herbal formulations to treat patients with cancer. Alkaloids are commonly known to present in SF as main active constituents. Here, we report that among the six characterized SF-derived quinolizidine alkaloids including sophoridine, aloperine, sophocarpine, matrine, oxymatrine and cytisine, aloperine exerted the most potent in vitro cytotoxic activity against the human cancer cell lines and oxymatrine exhibited selective anti-cancer activity against hepatocellular carcinoma HepG2 cells. Analysis of DNA fragmentation and PARP cleavage revealed that aloperine treatment for 48 hr induced apoptosis in HL-60 cells. In addition, autophagic formation of acidic vacuole was also observed in HL-60 cells exposed to aloperine. These results suggest that aloperine may be a novel contributor to the anti-cancer properties of SF.
Asunto(s)
Antineoplásicos/farmacología , Fitoterapia , Preparaciones de Plantas/farmacología , Quinolizidinas/farmacología , Sophora/química , Alcaloides/farmacología , Apoptosis/efectos de los fármacos , Azocinas/farmacología , Medicamentos Herbarios Chinos/farmacología , Electroforesis Capilar/métodos , Células HL-60 , Humanos , Concentración 50 Inhibidora , Células K562 , Piperidinas/farmacología , Raíces de Plantas/química , Quinolizinas/farmacología , MatrinasRESUMEN
OBJECTIVES: The reproductive activities of purified fractions from the root extract of Cnestis ferruginea was evaluated in rats. Phytochemical screening of the extract revealed the presence of alkaloids, tannins and anthraquinones. MATERIALS AND METHODS: Column chromatography produced 20 fractions which were reduced to 6 by thin layer chromatography. Nuclear magnetic resonance spectroscopy revealed quinolizidine alkaloids (fractions 3 and 4) as the active principles in the extract of Cnestis ferruginea. Each fraction of Cnestis ferruginea (0.1, 1 and 2 mgkg(-1)bw) was administered to rats by gavages for 60 days. RESULTS: All fractions caused significant reduction (p<0.05) in sperm counts, motility, viability, morphology and plasma levels of testosterone, luteinizing hormone and follicle stimulating hormone. Fractions 3 and 4 caused the highest reduction (p<0.001) in fertility, FSH and LH levels comparable to those of quinine sulphate. There was recovery after 60 days of withdrawal from the extracts. CONCLUSION: The results suggest that Cnestis ferruginea possesses reversible male antifertility effects. The active principles with these activities appear to be quinolizidine alkaloids.
Asunto(s)
Alcaloides/farmacología , Connaraceae/química , Fertilidad/efectos de los fármacos , Extractos Vegetales/farmacología , Quinolizidinas/farmacología , Animales , Cromatografía en Capa Delgada , Hormona Folículo Estimulante/sangre , Hormona Luteinizante/sangre , Espectroscopía de Resonancia Magnética , Masculino , Raíces de Plantas , Ratas , Recuento de Espermatozoides , Testículo/efectos de los fármacos , Testosterona/sangreRESUMEN
A series comprising hexane, ethyl acetate, ethanol and aqueous extracts from six common Chinese herbs (Carpesium abrotanoides, Melia toosendan, Cnidium monnieri, Vitex negundo, Stemona sp. and Sophora flavescens) was investigated for antifouling (AF) activity against cypris (cyprids) larvae of the barnacle Balanus albicostatus. All extracts tested except the aqueous extract from Stemona sp. significantly inhibited the settlement of cyprids, the most potent being the ethyl acetate extract of S. flavescens (EC(50) value 2.08 microg ml(-1)), from which an AF compound, identified as 2'-methoxykurarinone, was isolated using bioassay-guided procedures. Furthermore, the AF activity of this compound was found to be highly reversible and greater than that of the three other natural products from S. flavescens, namely matrine, oxymatrine and oxysophocarpine. These compounds have been used commercially in China for their pharmaceutical activities, but their AF activities have not previously been evaluated. Analysis of structure-activity relationships suggested that the N-1 nitrogen atom in matrine plays a crucial role in AF activity. Overall, the present findings indicate that herbal plants are a valuable source of novel AF agents.
Asunto(s)
Alcaloides , Medicamentos Herbarios Chinos/farmacología , Magnoliopsida/química , Quinolizidinas , Thoracica/efectos de los fármacos , Thoracica/crecimiento & desarrollo , Alcaloides/química , Alcaloides/farmacología , Animales , Asteraceae/química , Cnidium/química , Larva/efectos de los fármacos , Larva/crecimiento & desarrollo , Magnoliopsida/clasificación , Biología Marina , Melia/química , Quinolizidinas/química , Quinolizidinas/farmacología , Quinolizinas/química , Quinolizinas/farmacología , Sophora/química , Stemonaceae/química , Relación Estructura-Actividad , Vitex/química , MatrinasRESUMEN
Calia secundiflora (Ortega) Yakovlev (Fabaceae) is considered a medicinal plant in Mexico but has scarcely been used because of the toxicity of its quinolizidine alkaloids. Several quinolizidine alkaloids have shown bactericidal, nematicidal, and fungicidal activities. The purpose of this study was to identify the alkaloids in the seeds and evaluate the activity of the organic extract on several phytopathogenic fungi and bacteria. An in vitro bioassay was conducted with species of the following phytopathogenic fungi: Alternaria solani, Fusarium oxysporum and Monilia fructicola; and of the following bacteria Pseudomonas sp., Xanthomonas campestris and Erwinia carotovora. Cytisine, lupinine, anagyrine, sparteine, N-methylcytisine, 5,6-dehydrolupanine, and lupanine were identified by liquid chromatography-mass spectrometry in the extract of seeds; the most abundant compound of the extract was cytisine. It was observed that the crude extract of Calia secundiflora was moderately active on bacteria and more potent on phytopathogenic fungi. In contrast cytisine showed the opposite effects.
Asunto(s)
Antibacterianos/farmacología , Antifúngicos/farmacología , Fabaceae/química , Extractos Vegetales/química , Alcaloides/química , Alcaloides/aislamiento & purificación , Alcaloides/farmacología , Alternaria/efectos de los fármacos , Antibacterianos/aislamiento & purificación , Antifúngicos/aislamiento & purificación , Azocinas/farmacología , Candida/efectos de los fármacos , Cromatografía Liquida , Fusarium/efectos de los fármacos , Espectrometría de Masas , Pectobacterium carotovorum/efectos de los fármacos , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología , Pseudomonas/efectos de los fármacos , Quinolizidinas/aislamiento & purificación , Quinolizidinas/farmacología , Quinolizinas/farmacología , Semillas/química , Esparteína/farmacología , Xanthomonas campestris/efectos de los fármacosRESUMEN
Four new Daphniphyllum alkaloids, daphnioldhanines H-K ( 1- 4), along with 34 known alkaloids, were isolated from Daphniphyllum oldhami. The known alkaloid dehydrodaphnigraciline ( 5) is now reported as a natural product. Their structures were elucidated by spectroscopic methods, especially 2D NMR techniques. The effects against platelet aggregation of compounds 1, 3, and 5 were evaluated, and 3 showed stronger activity against platelet aggregation induced by PAF. This is the first report of quinolizidine alkaloids from the genus Daphniphyllum.
Asunto(s)
Alcaloides/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Plantas Medicinales/química , Inhibidores de Agregación Plaquetaria/aislamiento & purificación , Quinolizidinas/aislamiento & purificación , Saxifragaceae/química , Alcaloides/química , Alcaloides/farmacología , Cristalografía por Rayos X , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Conformación Molecular , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Inhibidores de Agregación Plaquetaria/química , Inhibidores de Agregación Plaquetaria/farmacología , Quinolizidinas/química , Quinolizidinas/farmacologíaRESUMEN
Two new phenanthroquinolizidine alkaloids, boehmeriasins A and B, were isolated from the aqueous ethanolic extract of Boehmeria siamensis Craib (Urticaceae) by bioassay-guided fractionation. Their structures were elucidated on the basis of spectral evidence. Boehmeriasin A possesses cytotoxic activity against 12 cell lines from 6 panels of cancer including lung cancer, colon cancer, breast cancer, prostate cancer, kidney cancer and leukemia with GI (50) between 0.2 and 100 ng/mL, whereas boehmeriasin B showed lower activity.